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Author Suagee-Bedore, J.K.; Linden, D.R.; Bennett-Wimbush, K. url  doi
openurl 
  Title Effect of Pen Size on Stress Responses of Stall-Housed Horses Receiving One Hour of Daily Turnout Type Journal Article
  Year 2021 Publication Journal of Equine Veterinary Science Abbreviated Journal J. Equine Vet. Sci.  
  Volume 98 Issue Pages (down) 103366  
  Keywords Agonistic behaviors; Cortisol; Group turnout; Paddock sizes  
  Abstract Group turnout provides important socializing opportunities for horses, particularly those that are primarily stalled. A high percentage of equine injuries occur during group turnout, which could partly be due to the physical constraints of fencing. To investigate appropriate paddock sizes for group turnouts, horses (n = 12) from a single herd were divided into groups of 4, stalled for 24 hours, and then turned out for 1 hour into one of three differently sized pens: 342, 263, and 184 m2 per horse. Groups rotated through pens across 3 days, receiving one treatment per day. Blood was sampled for cortisol concentrations at 08:00 hours each morning, and then at 15 and 60 minutes into the turn out sessions, and 60 minutes after return to individual stalls. Groups rotated through three turnout times: 09:00, 12:00, 14:00 hours. Counts of agonistic behaviors (chasing, contact biting, and kicking) and low-level threats (pinned ears, tail swishing, bite and kick threats) were recorded. When turned out in pens that provided 342 m2 per horse, horses exhibited reduced plasma cortisol concentrations by 15 minutes after turnout and at 1 hour after return to their stalls (P < .05). Horses in pens providing 184 m2 per horse exhibited greater agonistic (P < .001) and low-level threat (P < .01) behaviors than horses in larger pens. These data provide insight into appropriate pen sizes for horses from established herds. Providing at least 342 m2 per horse may reduce the chance of injury in horses accustomed to group turnout.  
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  ISSN 0737-0806 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6694  
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Author Tsong, T.Y. openurl 
  Title Conformational relaxations of urea- and guanidine hydrochloride-unfolded ferricytochrome c Type Journal Article
  Year 1977 Publication The Journal of Biological Chemistry Abbreviated Journal J Biol Chem  
  Volume 252 Issue 24 Pages (down) 8778-8780  
  Keywords *Cytochrome c Group; Guanidines/*pharmacology; Protein Conformation/drug effects; Spectrometry, Fluorescence; Urea/*pharmacology  
  Abstract Several recent studies of protein the unfolded proteins. In urea- and guanidine HCl-unfolded ferricytochrome c (horse heart), an acid-induced spin state transformation of the heme group has been detected by the heme absorptions, Trp-59 fluorescence, and the intrinsic viscosity of protein. Kinetics of this second conformational transition, by the temperature jump and stopped flow methods, are complex. One rapid reaction (tau1), pH-independent, occurs in a 50-mus range; the second reaction (tau2), in a 1-ms range, depends linearly upon pH and is faster at the alkaline side; a third reaction (tau3), in a 1-s range, shows a sigmoidal transition at pH 5.1 and is faster at the acidic side. The results are consistent with a kinetic scheme which involves protein conformational changes in the transformation of the heme coordination state. The kinetics, along with previous equilibrium studies, indicate that ligand or charge interactions within a protein molecule are not completely prohibited even in strongly denaturing conditions, such as in high concentrations of urea and guanidine HCl. Thus, local structures of peptide chain associated with these interactions can exist in the unfolded protein.  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0021-9258 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:200618 Approved no  
  Call Number refbase @ user @ Serial 3882  
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Author Abbruzzetti, S.; Viappiani, C.; Small, J.R.; Libertini, L.J.; Small, E.W. openurl 
  Title Kinetics of histidine deligation from the heme in GuHCl-unfolded Fe(III) cytochrome C studied by a laser-induced pH-jump technique Type Journal Article
  Year 2001 Publication Journal of the American Chemical Society Abbreviated Journal J Am Chem Soc  
  Volume 123 Issue 27 Pages (down) 6649-6653  
  Keywords Animals; *Bacterial Proteins; Cytochrome c Group/*chemistry; Guanidine/*chemistry; Heme/*chemistry; Histidine/*chemistry; Horses; Hydrogen-Ion Concentration; Kinetics; *Lasers; Ligands; Protein Folding  
  Abstract We have developed an instrumental setup that uses transient absorption to monitor protein folding/unfolding processes following a laser-induced, ultrafast release of protons from o-nitrobenzaldehyde. The resulting increase in [H(+)], which can be more than 100 microM, is complete within a few nanoseconds. The increase in [H(+)] lowers the pH of the solution from neutrality to approximately 4 at the highest laser pulse energy used. Protein structural rearrangements can be followed by transient absorption, with kinetic monitoring over a broad time range (approximately 10 ns to 500 ms). Using this pH-jump/transient absorption technique, we have examined the dissociation kinetics of non-native axial heme ligands (either histidine His26 or His33) in GuHCl-unfolded Fe(III) cytochrome c (cyt c). Deligation of the non-native ligands following the acidic pH-jump occurs as a biexponential process with different pre-exponential factors. The pre-exponential factors markedly depend on the extent of the pH-jump, as expected from differences in the pK(a) values of His26 and His33. The two lifetimes were found to depend on temperature but were not functions of either the magnitude of the pH-jump or the pre-pulse pH of the solution. The activation energies of the deligation processes support the suggestion that GuHCl-unfolded cyt c structures with non-native histidine axial ligands represent kinetic traps in unfolding.  
  Address Dipartimento di Fisica, Universita di Parma, Istituto Nazionale per la Fisica della Materia, 43100 Parma, Italy  
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  Series Volume Series Issue Edition  
  ISSN 0002-7863 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:11439052 Approved no  
  Call Number Equine Behaviour @ team @ Serial 3788  
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Author Curtis, S.E.; Stricklin, W.R. openurl 
  Title The importance of animal cognition in agricultural animal production systems: an overview Type Journal Article
  Year 1991 Publication Journal of Animal Science Abbreviated Journal J. Anim Sci.  
  Volume 69 Issue 12 Pages (down) 5001-5007  
  Keywords *Agriculture; Animal Population Groups/*psychology; *Animal Welfare; Animals; *Behavior, Animal; *Cognition; Heat; Helplessness, Learned; Housing, Animal/standards; Immobilization; Nesting Behavior; Pain/psychology/veterinary  
  Abstract To describe and then fulfill agricultural animals' needs, we must learn more about their fundamental psychological and behavioral processes. How does this animal feel? Is that animal suffering? Will we ever be able to know these things? Scientists specializing in animal cognition say that there are numerous problems but that they can be overcome. Recognition by scientists of the notion of animal awareness has been increasing in recent years, because of the work of Griffin and others. Feeling, thinking, remembering, and imagining are cognitive processes that are factors in the economic and humane production of agricultural animals. It has been observed that the animal welfare debate depends on two controversial questions: Do animals have subjective feelings? If they do, can we find indicators that reveal them? Here, indirect behavioral analysis approaches must be taken. Moreover, the linear additivity of several stressor effects on a variety of animal traits suggests that some single phenomenon is acting as a “clearinghouse” for many or all of the stresses acting on an animal at any given time, and this phenomenon might be psychological stress. Specific situations animals may encounter in agricultural production settings are discussed with respect to the animals' subjective feelings.  
  Address University of Illinois, Urbana 61801  
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  Language English Summary Language Original Title  
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  Series Volume Series Issue Edition  
  ISSN 0021-8812 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:1808193 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2754  
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Author Dyson, H.J.; Beattie, J.K. openurl 
  Title Spin state and unfolding equilibria of ferricytochrome c in acidic solutions Type Journal Article
  Year 1982 Publication The Journal of Biological Chemistry Abbreviated Journal J Biol Chem  
  Volume 257 Issue 5 Pages (down) 2267-2273  
  Keywords Animals; *Cytochrome c Group; Electron Spin Resonance Spectroscopy; Heme; Horses; Hydrogen-Ion Concentration; Kinetics; Ligands; Myocardium; Protein Binding; Protein Conformation; Spectrophotometry; Temperature  
  Abstract Equilibrium, stopped flow, and temperature-jump spectrophotometry have been used to identify processes in the unfolding of ferricytochrome c in acidic aqueous solutions. A relaxation occurring in approximately 100 microseconds involves perturbation of a spin-equilibrium between two folded conformers of the protein with methionine-80 coordinated or dissociated from the heme iron. The protein unfolds more slowly, in milliseconds, with dissociation and protonation of histidine-18. These two transitions appear cooperative in equilibrium measurements at low (0.01 M) ionic strength, but are separated at higher (0.10 M) ionic strength. They are resolved under both conditions in the dynamic measurements. The spin-equilibrium description permits a unified explanation of a number of properties of ferricytochrome c in acidic aqueous solutions.  
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  Series Volume Series Issue Edition  
  ISSN 0021-9258 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:6277891 Approved no  
  Call Number Equine Behaviour @ team @ Serial 3807  
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Author Saigo, S. openurl 
  Title A transient spin-state change during alkaline isomerization of ferricytochrome c Type Journal Article
  Year 1981 Publication Journal of Biochemistry Abbreviated Journal J Biochem (Tokyo)  
  Volume 89 Issue 6 Pages (down) 1977-1980  
  Keywords Animals; *Cytochrome c Group; Horses; Hydrogen-Ion Concentration; Isomerism; Kinetics; Myocardium/enzymology; Oxidation-Reduction; Spectrophotometry  
  Abstract Kinetic difference spectra during the alkaline isomerization of ferricytochrome c were obtained by the pH-jump method in the range of 540 to 655 nm. The spectrum of the transient intermediate, which appears during the course of the isomerization, was reproduced from the spectra. The intermediate showed an intense absorption band at 600 nm, indicating that it is a high spin or mixed spin species. This is in contrast to the stable neutral and alkaline forms which are low spin species. The transient spin-state change during the isomerization was also observed upon rapid oxidation of ferrocytochrome c at alkaline pH.  
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  ISSN 0021-924X ISBN Medium  
  Area Expedition Conference  
  Notes PMID:6270075 Approved no  
  Call Number Equine Behaviour @ team @ Serial 3808  
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Author King, A.J.; Douglas, C.M.S.; Huchard, E.; Isaac, N.J.B.; Cowlishaw, G. url  doi
openurl 
  Title Dominance and affiliation mediate despotism in a social primate Type Journal Article
  Year 2008 Publication Current Biology : CB Abbreviated Journal Curr Biol  
  Volume 18 Issue 23 Pages (down) 1833-1838  
  Keywords Animals; *Authoritarianism; Behavior, Animal/*physiology; Cooperative Behavior; *Decision Making; Feeding Behavior; Female; *Group Processes; Male; Papio ursinus/*psychology; *Social Dominance  
  Abstract Group-living animals routinely have to reach a consensus decision and choose between mutually exclusive actions in order to coordinate their activities and benefit from sociality. Theoretical models predict “democratic” rather than “despotic” decisions to be widespread in social vertebrates, because they result in lower “consensus costs”-the costs of an individual foregoing its optimal action to comply with the decision-for the group as a whole. Yet, quantification of consensus costs is entirely lacking, and empirical observations provide strong support for the occurrence of both democratic and despotic decisions in nature. We conducted a foraging experiment on a wild social primate (chacma baboons, Papio ursinus) in order to gain new insights into despotic group decision making. The results show that group foraging decisions were consistently led by the individual who acquired the greatest benefits from those decisions, namely the dominant male. Subordinate group members followed the leader despite considerable consensus costs. Follower behavior was mediated by social ties to the leader, and where these ties were weaker, group fission was more likely to occur. Our findings highlight the importance of leader incentives and social relationships in group decision-making processes and the emergence of despotism.  
  Address Institute of Zoology, Zoological Society of London, Regent's Park, London, NW1 4RY, UK. andrew.king@ioz.ac.uk  
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  ISSN 0960-9822 ISBN Medium  
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  Notes PMID:19026539 Approved no  
  Call Number Equine Behaviour @ team @ Serial 5124  
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Author Ridge, J.A.; Baldwin, R.L.; Labhardt, A.M. openurl 
  Title Nature of the fast and slow refolding reactions of iron(III) cytochrome c Type Journal Article
  Year 1981 Publication Biochemistry Abbreviated Journal Biochemistry  
  Volume 20 Issue 6 Pages (down) 1622-1630  
  Keywords Animals; Ascorbic Acid; *Cytochrome c Group; Guanidines; Horses; Kinetics; Oxidation-Reduction; Protein Conformation; Spectrum Analysis  
  Abstract The fast and slow refolding reactions of iron(III) cytochrome c (Fe(III) cyt c), previously studied by Ikai et al. (Ikai, A., Fish, W. W., & Tanford, C. (1973) J. Mol. Biol. 73, 165--184), have been reinvestigated. The fast reaction has the major amplitude (78%) and is 100-fold faster than the slow reaction in these conditions (pH 7.2, 25 degrees C, 1.75 M guanidine hydrochloride). We show here that native cyt c is the product formed in the fast reaction as well as in the slow reaction. Two probes have been used to test for formation of native cyt c. absorbance in the 695-nm band and rate of reduction of by L-ascorbate. Different unfolded species (UF, US) give rise to the fast and slow refolding reactions, as shown both by refolding assays at different times after unfolding (“double-jump” experiments) and by the formation of native cyt c in each of the fast and slow refolding reactions. Thus the fast refolding reaction is UF leads to N and the slow refolding reaction is Us leads to N, where N is native cyt c, and there is a US in equilibrium UF equilibrium in unfolded cyt c. The results are consistent with the UF in equilibrium US reaction being proline isomerization, but this has not yet been tested in detail. Folding intermediates have been detected in both reactions. In the UF leads to N reaction, the Soret absorbance change precedes the recovery of the native 695-nm band spectrum, showing that Soret absorbance monitors the formation of a folding intermediate. In the US leads to N reaction an ascorbate-reducible intermediate has been found at an early stage in folding and the Soret absorbance change occurs together with the change at 695 nm as N is formed in the final stage of folding.  
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  ISSN 0006-2960 ISBN Medium  
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  Notes PMID:6261802 Approved no  
  Call Number Equine Behaviour @ team @ Serial 3809  
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Author Meunier, H.; Leca, J.B.; Deneubourg, J.L.; Petit, O. doi  openurl
  Title Group movement decisions in capuchin monkeys: the utility of an experimental study and a mathematical model to explore the relationship between individual and collective behaviours Type Journal Article
  Year 2006 Publication Behaviour Abbreviated Journal Behaviour  
  Volume 143 Issue Pages (down) 1511-1527  
  Keywords animal society – collective decision-making – primates – group movement – mathematical modeling  
  Abstract In primate groups, collective movements are typically described as processes dependent on leadership mechanisms. However, in some species, decision-making includes negotiations and distributed leadership. These facts suggest that simple underlying processes may explain certain decision mechanisms during collective movements. To study such processes, we have designed experiments on white-faced capuchin monkeys (Cebus capucinus) during which we provoked collective movements involving a binary choice. These experiments enabled us to analyse the spatial decisions of individuals in the group. We found that the underlying process includes anonymous mimetism, which means that each individual may influence all members of the group. To support this result, we created a mathematical model issued from our experimental data. A totally anonymous model does not fit perfectly with our experimental distribution. A more individualised model, which takes into account the specific behaviour of social peripheral individuals, revealed the validity of the mimetism hypothesis. Even though white-faced capuchins have complex cognitive abilities, a coexistence of anonymous and social mechanisms appears to influence their choice of direction during collective movements. The present approach may offer vital insights into the relationships between individual behaviours and their emergent collective acts.  
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  Notes Approved no  
  Call Number Serial 2066  
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Author Boogert, N.J.; Reader, S.M.; Hoppitt, W.; Laland, K.N. url  doi
openurl 
  Title The origin and spread of innovations in starlings Type Journal Article
  Year 2008 Publication Animal Behaviour Abbreviated Journal Anim. Behav.  
  Volume 75 Issue 4 Pages (down) 1509-1518  
  Keywords diffusion dynamics; dominance; foraging; group; innovation; neophobia; social learning; social network; starling; Sturnus vulgaris  
  Abstract There are numerous reports of novel learned behaviour patterns in animal populations, yet the factors influencing the invention and spread of these innovations remain poorly understood. Here we investigated to what extent the pattern of spread of innovations in captive groups of starlings, Sturnus vulgaris, could be predicted by knowledge of individual and social group variables, including association patterns, social rank orders, measures of neophobia and asocial learning performance. We presented small groups of starlings with a series of novel extractive foraging tasks and recorded the latency for each bird to contact and solve each task, as well as the orders of contacting and solving. We then explored which variables best predicted the observed diffusion patterns. Object neophobia and social rank measures characterized who was the first of the group to contact the novel foraging tasks, and the subsequent spread of contacting tasks was associated with latency to feed in a novel environment. Asocial learning performance, measured in isolation, predicted who was the first solver of the novel foraging tasks in each group. Association patterns did not predict the spread of solving. Contact latency and solving duration were negatively correlated, consistent with social learning underlying the spread of solving. Our findings indicate that we can improve our understanding of the diffusion dynamics of innovations in animal groups by investigating group-dependent and individual variables in combination. We introduce novel methods for exploring predictors of the origin and spread of behavioural innovations that could be widely applied.  
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  ISSN 0003-3472 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6036  
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