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Author  |
Hinchcliff, K.W.; Kohn, C.W.; Geor, R.; McCutcheon, L.J.; Foreman, J.; Andrews, F.M.; Allen, A.K.; White, S.L.; Williamson, L.H.; Maykuth, P.L. |
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Title |
Acid:base and serum biochemistry changes in horses competing at a modified 1 Star 3-day-event |
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Journal Article |
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Year |
1995 |
Publication |
Equine Veterinary Journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
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Volume |
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Issue |
20 |
Pages |
105-110 |
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Keywords |
*Acid-Base Equilibrium; Animals; Blood Proteins/analysis; Body Water/metabolism; Carbon Dioxide/blood; Electrolytes/*blood; Female; Hematocrit/veterinary; Homeostasis; Horses/*blood/physiology; Hydrogen-Ion Concentration; Male; Physical Conditioning, Animal/*physiology |
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Abstract |
We examined the effects of participation in each of 3 modifications of Day 2 of a 3-day-event on blood and serum variables indicative of hydration, acid:base status and electrolyte homeostasis of horses. Three groups of horses – 8 European (E) horses and 2 groups each of 9 North American horses performed identical Days 1 (dressage) and 3 (stadium jumping) of a 3-day-event. E horses and one group of the North American horses (TD) performed modifications of Day 2 of a 1 Star 3-day-event and the other group of North American horses (HT) performed a Horse Trial on Day 2. Jugular venous blood was collected from each horse on the morning of Day 2 before any warm-up activity, between 4 min 55 s and 5 min 15 s after Phase D and the following morning. Eight E horses, 5 TD horses and 8 HT horses completed the trials. There were few significant differences in acid:base or serum biochemistry variables detected among horses performing either 2 variations of the Speed and Endurance day of a 1 Star 3-day-event, or a conventional Horse Trial. Failure to detect differences among groups may have been related to the low statistical power associated with the small number of horses, especially in the TD group, variation in quality of horses among groups and the different times of the day at which the E horses competed. Differences detected among time points were usually common to all groups and demonstrated metabolic acidosis with a compensatory respiratory alkalosis, a reduction in total body water and cation content, and hypocalcaemia. Importantly, horses of all groups did not replenish cation, chloride, and calcium deficits after 14-18 h of recovery. |
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Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210-1089, USA |
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PMID:8933092 |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3740 |
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Author |
Holzapfel, W.H.; Botha, S.J. |
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Title |
Physiology of Sporolactobacillus strains isolated from different habitats and the indication of in vitro antagonism against Bacillus species |
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Journal Article |
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Year |
1988 |
Publication |
International Journal of Food Microbiology |
Abbreviated Journal |
Int J Food Microbiol |
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Volume |
7 |
Issue |
2 |
Pages |
161-168 |
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Keywords |
Animals; Bacillaceae/isolation & purification/*physiology; Bacillus/*physiology; Cattle; *Ecology; Feces/*microbiology; Food Microbiology; Horses; Sewage; Sheep; Water Microbiology |
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In an ecological study only low numbers of Sporolactobacillus were found in habitats such as the faeces of herbivores, the rumen of cattle and the final waste water of an abattoir. Their presence in the final waste water of an abattoir indicates their possible association with food, and, more specifically, with meat. Differences were found in some physiological characteristics. One isolate (L2404) differed from the authentic Sporolactobacillus ATCC 15538 by its inability to ferment inulin, its growth in presence of 6.5% NaCl and in 0.2% tellurite, by the isomer(s) of lactic acid produced and the mol% G + G in the DNA. One Sporolactobacillus isolate (L2407) showed antagonism against Bacillus cereus, Bacillus cereus var, mycoides, Bacillus megaterium and Bacillus subtilis. |
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Department of Microbiology and Plant Pathology, University of Pretoria, Republic of South Africa |
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0168-1605 |
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PMID:3275317 |
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Call Number |
Equine Behaviour @ team @ |
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2675 |
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Houpt, K.A.; Eggleston, A.; Kunkle, K.; Houpt, T.R. |
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Title |
Effect of water restriction on equine behaviour and physiology |
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Journal Article |
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Year |
2000 |
Publication |
Equine veterinary journal |
Abbreviated Journal |
Equine Vet J |
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32 |
Issue |
4 |
Pages |
341-344 |
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Keywords |
Animals; *Behavior, Animal; Blood Proteins/analysis; Energy Intake; Female; Horse Diseases/physiopathology; Horses/*physiology; Osmolar Concentration; Pregnancy; Stress/veterinary; Video Recording; Water Deprivation/*physiology |
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Six pregnant mares were used to determine what level of water restriction causes physiological and/or behavioural changes indicative of stress. Nonlegume hay was fed ad libitum. During the first week of restriction, 5 l water/100 kg bwt was available, during the second week 4 l/100 kg bwt and, during the third week, 3 l/100 kg bwt. Ad libitum water intake was 6.9 l/100 kg bwt; at 3 l/100 kg bwt water intake was 42% of this. Daily hay intake fell significantly with increasing water restriction from 12.9 +/- 0.75 kg to 8.3 +/- 0.54 kg; bodyweight fell significantly for a total loss of 48.5 +/- 8.3 kg in 3 weeks. Daily blood samples were analysed; osmolality rose significantly with increasing water restriction from 282 +/- 0.7 mosmols/kg to 293.3 +/- 0.8 mosmols/kg bwt, but plasma protein and PCV did not change significantly. Cortisol concentrations fell from 8.1 ng/ml to 6.4 ng/ml over the 3 week period. Aldosterone fell from 211.3 +/- 74.2 pg/ml to 92.5 +/- 27.5 pg/ml at the end of the first week. The behaviour of 4 of the 6 mares was recorded 24 h/day for the duration of the study. The only significant difference was in time spent eating, which decreased with increasing water restriction from 46 +/- 3% to 30 +/- 3%. It is concluded that water restriction to 4 l/100 kg bwt dehydrates pregnant mares and may diminish their welfare, but is not life- or pregnancy-threatening. |
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Department of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA |
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0425-1644 |
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PMID:10952384 |
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no |
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Call Number |
refbase @ user @ |
Serial |
32 |
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Author |
Houpt, K.A.; Perry, P.J.; Hintz, H.F.; Houpt, T.R. |
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Title |
Effect of meal frequency on fluid balance and behavior of ponies |
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Journal Article |
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Year |
1988 |
Publication |
Physiology & behavior |
Abbreviated Journal |
Physiol. Behav. |
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42 |
Issue |
5 |
Pages |
401-407 |
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Animals; Behavior, Animal/*physiology; Blood Proteins/analysis; *Eating; Female; Hematocrit; Horses/blood/*physiology; Osmolar Concentration; *Water-Electrolyte Balance |
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Abstract |
Twelve ponies were fed their total daily ration either as one large meal or divided into six small meals. Pre- and post-feeding behavior was recorded six times a day. Blood samples were taken for 30 min before and two hr after the meal. Plasma protein increased from 7.0 to a peak of 7.3 g/dl with small meals and from 7.3 to 8.1 g/dl with large meals, and returned to pre-feeding levels by 90 min post-feeding. Hematocrit rose from 33.3 to 34.1% with small meals and from 33.0 to 36.0% with large meals. These rapid and short-lived increases indicate a decrease in plasma volume. Plasma osmolality rose with feeding from 283 to 285 mosmoles/kg with small meals and from 281 to 288 mosmoles/kg with large meals. Water availability had no significant effect on blood changes. Digestibility and rate of passage were measured with chromic oxide, but there were no differences. Vocalizing (neighing) and walking occurred more often before than after feeding, while eating bedding and engaging in other oral behaviors were more frequent after feeding. |
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Department of Physiology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853 |
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0031-9384 |
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PMID:3393599 |
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refbase @ user @ |
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47 |
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Houpt, T.R.; Houpt, K.A. |
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Title |
Nitrogen conservation by ponies fed a low -protein ration |
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Journal Article |
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Year |
1971 |
Publication |
American journal of veterinary research |
Abbreviated Journal |
Am J Vet Res |
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32 |
Issue |
4 |
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579-588 |
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Administration, Oral; Amino Acids/biosynthesis; Animals; Anti-Infective Agents/pharmacology; Body Weight/drug effects; Dietary Proteins/*pharmacology; Horses/*metabolism; Nitrogen/*metabolism; Urea/administration & dosage/antagonists & inhibitors/metabolism; Water/metabolism |
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0002-9645 |
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PMID:5110116 |
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refbase @ user @ |
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62 |
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Author |
Maury, M.; Murphy, K.; Kumar, S.; Mauerer, A.; Lee, G. |
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Title |
Spray-drying of proteins: effects of sorbitol and trehalose on aggregation and FT-IR amide I spectrum of an immunoglobulin G |
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Journal Article |
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Year |
2005 |
Publication |
European Journal of Pharmaceutics and Biopharmaceutics |
Abbreviated Journal |
Eur. J. Pharm. Biopharm. |
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Volume |
59 |
Issue |
2 |
Pages |
251-261 |
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Keywords |
Immunoglobulin; Spray-drying; Stabilization; Sorbitol; Trehalose; Water replacement |
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Abstract |
An immunoglobulin G (IgG) was spray-dried on a Büchi 190 laboratory spray-dryer at inlet and outlet air temperatures of 130 and 190°C, respectively. The IgG solution contains initially 115mg/ml IgG plus 50mg/ml sorbitol. After dialysis, at least 80% of low molecular weight component was removed. After spray-drying the dialyzed IgG and immediate redissolution of the powder, an increase in aggregates from 1 to 17% occurred. A major shift towards increase β-sheet structure was detected in the spray-dried solid, which, however, reverted to native structure on redissolution of the powder. A correlation between aggregation determined by size exclusion chromatography and alterations in secondary structure determined by Fourier transformation infra-red spectroscopy could not therefore be established. On spray-drying a non-dialyzed, sorbitol-containing IgG only some 0.7% aggregates were formed. The sorbitol is therefore evidently able to stabilize partially the IgG during the process of spray-drying. Addition of trehalose to the liquid feed produced quantitatively the same stabilizing action on the IgG during spray-drying as did the sorbitol. This finding again points towards a water replacement stabilization mechanism. The IgG spray-dried powder prepared from the dialyzed liquid feed showed continued substantial aggregation on dry storage at 25°C. This was substantially less in the non-dialyzed, sorbitol-containing spray-dried powder. Addition of trehalose to both dialyzed and non-dialyzed system produced substantial improvement in storage stability and reduction in aggregate formation in storage. The quantitative stabilizing effect of the trehalose was only slightly higher than that of the sorbitol. Taken together, these results indicate that both the sorbitol and trehalose stabilize the IgG primarily by a water replacement mechanism rather than by glassy immobilization. The relevance of this work is its questioning of the importance of the usually considered dominance of glassy stabilization of protein in dried systems of high glass transition temperature, such as trehalose. The low glass transition temperature sorbitol produces almost equal process and storage stability in this case. |
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0939-6411 |
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Equine Behaviour @ team @ |
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6515 |
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Author |
McDonnell, S.M.; Freeman, D.A.; Cymbaluk, N.F.; Schott, H.C. 2nd; Hinchcliff, K.; Kyle, B. |
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Title |
Behavior of stabled horses provided continuous or intermittent access to drinking water |
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Journal Article |
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Year |
1999 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
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60 |
Issue |
11 |
Pages |
1451-1456 |
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Keywords |
Aggression; Animal Husbandry; Animals; Behavior, Animal/*physiology; Drinking Behavior; Feeding Behavior; Female; Horses/*physiology/*psychology; Housing, Animal; Pregnancy; Pregnancy, Animal/physiology/*psychology; Social Behavior; Videotape Recording; *Water Supply |
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OBJECTIVE: To compare quantitative measures and clinical assessments of behavior as an indication of psychologic well-being of stabled horses provided drinking water continuously or via 1 of 3 intermittent delivery systems. ANIMALS: 22 Quarter Horse (QH) or QH-crossbred mares and 17 Belgian or Belgian-crossbred mares (study 1) and 24 QH or QH-crossbred mares and 18 Belgian or Belgian-crossbred mares (study 2). PROCEDURE: Stabled horses were provided water continuously or via 1 of 3 intermittent water delivery systems in 2 study periods during a 2-year period. Continuous 24-hour videotaped samples were used to compare quantitative measures and clinical assessments of behavior among groups provided water by the various water delivery systems. RESULTS: All horses had clinically normal behavior. Significant differences in well being were not detected among groups provided water by the various delivery systems. CONCLUSIONS AND CLINICAL RELEVANCE: Various continuous and intermittent water delivery systems can provide adequately for the psychologic well-being of stabled horses. |
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New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348, USA |
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0002-9645 |
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PMID:10566826 |
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Call Number |
refbase @ user @ |
Serial |
1928 |
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Author |
Mejdell, C.M.; Simensen, E.; Boe, K.E. |
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Title |
Is snow a sufficient source of water for horses kept outdoors in winter? A case report |
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Journal Article |
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Year |
2005 |
Publication |
Acta Veterinaria Scandinavica |
Abbreviated Journal |
Acta Vet Scand |
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Volume |
46 |
Issue |
1-2 |
Pages |
19-22 |
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Keywords |
Animals; *Drinking Behavior; *Horses; Norway; Osmolar Concentration; Snow; *Thirst; *Water Supply |
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Due to extreme weather conditions, a flock of outwintered Icelandic horses had to manage for several days on snow as the source of free water. They were fed grass silage ad lib, and any change in feed consumption was not observed. After nine days, blood samples were taken and analysed for plasma osmolality, they were subjected to a simple clinical examination, and offered drinking water. Osmolality levels were within normal limits and mean value did not differ significantly from samples which previously were taken of the same individuals. The general condition of the horses was normal, with no signs of clinical dehydration or disease. The horses showed very little interest for the offered drinking water. This suggests that in cold winter weather, horses being fed grass silage and adjusted to eat snow, can manage for several days with snow substituting liquid water without their physiology and welfare being challenged. |
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National Centre for Veterinary Contract Research and Commercial Services, Ltd |
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0044-605X |
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PMID:16108209 |
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Call Number |
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Serial |
1886 |
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Author |
Mrosovsky, N.; Shettleworth, S.J. |
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Title |
Further studies of the sea-finding mechanism in green turtle hatchlings |
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Journal Article |
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Year |
1974 |
Publication |
Behaviour |
Abbreviated Journal |
Behaviour |
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51 |
Issue |
3-4 |
Pages |
195-208 |
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Keywords |
Animals; *Animals, Newborn/physiology; Contact Lenses; Locomotion; *Orientation; Retina/physiology; *Turtles/physiology; Visual Fields; *Visual Perception; Water |
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0005-7959 |
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PMID:4447586 |
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refbase @ user @ |
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389 |
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Mrosovsky, N.; Shettleworth, S.J. |
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Title |
Wavelength preferences and brightness cues in the water finding behaviour of sea turtles |
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Journal Article |
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Year |
1968 |
Publication |
Behaviour |
Abbreviated Journal |
Behaviour |
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32 |
Issue |
4 |
Pages |
211-257 |
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Keywords |
Animals; *Behavior, Animal; *Color Perception; Cues; Light; *Turtles; Water |
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0005-7959 |
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PMID:5717260 |
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Call Number |
refbase @ user @ |
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391 |
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