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Author Touma, C.; Sachser, N.; Mostl, E.; Palme, R. openurl 
  Title Effects of sex and time of day on metabolism and excretion of corticosterone in urine and feces of mice Type Journal Article
  Year 2003 Publication General and Comparative Endocrinology Abbreviated Journal Gen Comp Endocrinol  
  Volume 130 Issue 3 Pages 267-278  
  Keywords Animals; Chromatography, High Pressure Liquid; Circadian Rhythm/*physiology; Corticosterone/*metabolism/urine; Feces/*chemistry; Female; Immunoenzyme Techniques; Kinetics; Male; Mice; Mice, Inbred C57BL; Reference Values; Sex Factors; Stress/metabolism; Time Factors; Tritium  
  Abstract Non-invasive techniques to monitor stress hormones in small animals like mice offer several advantages and are highly demanded in laboratory as well as in field research. Since knowledge about the species-specific metabolism and excretion of glucocorticoids is essential to develop such a technique, we conducted radiometabolism studies in mice (Mus musculus f. domesticus, strain C57BL/6J). Each mouse was injected intraperitoneally with 740 kBq of 3H-labelled corticosterone and all voided urine and fecal samples were collected for five days. In a first experiment 16 animals (eight of each sex) received the injection at 9 a.m., while eight mice (four of each sex) were injected at 9 p.m. in a second experiment. In both experiments radioactive metabolites were recovered predominantly in the feces, although males excreted significantly higher proportions via the feces (about 73%) than females (about 53%). Peak radioactivity in the urine was detected within about 2h after injection, while in the feces peak concentrations were observed later (depending on the time of injection: about 10h postinjection in experiment 1 and about 4h postinjection in experiment 2, thus proving an effect of the time of day). The number and relative abundance of fecal [3H]corticosterone metabolites was determined by high performance liquid chromatography (HPLC). The HPLC separations revealed that corticosterone was extensively metabolized mainly to more polar substances. Regarding the types of metabolites formed, significant differences were found between males and females, but not between the experiments. Additionally, the immunoreactivity of these metabolites was assessed by screening the HPLC fractions with four enzyme immunoassays (EIA). However, only a newly established EIA for 5alpha-pregnane-3beta,11beta,21-triol-20-one (measuring corticosterone metabolites with a 5alpha-3beta,11beta-diol structure) detected several peaks of radioactive metabolites with high intensity in both sexes, while the other EIAs showed only minor immunoreactivity. Thus, our study for the first time provides substantial information about metabolism and excretion of corticosterone in urine and feces of mice and is the first demonstrating a significant impact of the animals' sex and the time of day. Based on these data it should be possible to monitor adrenocortical activity non-invasively in this species by measuring fecal corticosterone metabolites with the newly developed EIA. Since mice are extensively used in research world-wide, this could open new perspectives in various fields from ecology to behavioral endocrinology.  
  Address Department of Behavioral Biology, Institute of Neuro and Behavioral Biology, University of Muenster, Badestrasse 9, D-48149 Muenster, Germany. touma@uni-muenster.de  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
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  ISSN (up) 0016-6480 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:12606269 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4086  
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Author Schmidt, R.; Amrhein, V.; Kunc, H.P.; Naguib, M. doi  openurl
  Title The day after: effects of vocal interactions on territory defence in nightingales Type Journal Article
  Year 2007 Publication The Journal of Animal Ecology Abbreviated Journal T. J. Anim. Ecol.  
  Volume 76 Issue 1 Pages 168-173  
  Keywords Aggression; Animals; Male; Songbirds/*physiology; *Territoriality; Time Factors; Vocalization, Animal/*physiology  
  Abstract 1. Models on territory acquisition and tenure predict that territorial animals benefit by adjusting territorial defence behaviour to previous challenges they had experienced within the socially complex environment of communication networks. 2. Here, we addressed such issues of social cognition by investigating persisting effects of vocal contests on territory defence behaviour in nightingales Luscinia megarhynchos (Brehm). 3. Using interactive playback during nocturnal song of subjects, a rival was simulated to countersing either aggressively (by song overlapping) or moderately (by song alternating) from outside the subjects' territory. Thereby, the time-specific singing strategy provided an experimentally controlled source of information on the motivation of an unfamiliar rival. 4. Expecting that nightingales integrate information with time, the same rival was simulated to return as a moderately singing intruder on the following morning. 5. The results show that the vigour with which male nightingales responded to the simulated intrusion of an opponent during the day depended on the nature of the nocturnal vocal interaction experienced several hours before. 6. Males that had received the song overlapping playback the preceding night approached the simulated intruder more quickly and closer and sang more songs near the loudspeaker than did males that had received a song alternating playback. 7. This adjustment of territory defence strategies depending on information from prior signalling experience suggests that integrating information with time plays an important part in territory defence by affecting a male's decision making in a communication network.  
  Address Department of Animal Behaviour, Bielefeld University, PO Box 100 131, D-33501 Bielefeld, Germany. rouven.schmidt@uni-bielefeld.de  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
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  ISSN (up) 0021-8790 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:17184365 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2749  
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Author Crowell-Davis, S.L.; Houpt, K.A.; Carnevale, J. openurl 
  Title Feeding and drinking behavior of mares and foals with free access to pasture and water Type Journal Article
  Year 1985 Publication Journal of animal science Abbreviated Journal J. Anim Sci.  
  Volume 60 Issue 4 Pages 883-889  
  Keywords Animals; *Drinking Behavior; *Feeding Behavior; Female; Horses/*physiology; Male; Poaceae; Seasons; Temperature; Time Factors  
  Abstract The feeding and drinking behavior of 11 mares and 15 foals living on pasture with free access to water was recorded during 2,340 15-min focal samples taken over 2 yr. Lactating mares on pasture spent about 70% of the day feeding. Foals began feeding on their first day of life. As they grew older, they spent progressively more time feeding, but still spent only 47 +/- 6% of the time feeding by 21 wk of age. Foals fed primarily during the early morning and evening. While grass formed the major proportion of the diet of both foals and mares, they also ate clay, humus, feces, bark, leaves and twigs. Almost all feeding by foals was done while their mothers were feeding. Movement to water sources was frequently, but not invariably, carried out by an entire herd. Frequency (P = .005) but not duration (P greater than .05) of drinking bouts by mares increased as the temperature increased. Frequency was greatest at 30 to 35 C, at which temperature mares drank once every 1.8 h. Frequency of drinking varied with the time of day (P less than .01), being rarest during the early morning (0500 to 0900 h eastern daylight time) and most frequent during the afternoon (1300 to 1700 h). Drinking by foals was very rare. The youngest age at which a foal was observed to drink was 3 wk, and 8 of 15 foals were never observed to drink before weaning.  
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  ISSN (up) 0021-8812 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:3988655 Approved no  
  Call Number refbase @ user @ Serial 54  
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Author Gavrilova, O.; Haluzik, M.; Matsusue, K.; Cutson, J.J.; Johnson, L.; Dietz, K.R.; Nicol, C.J.; Vinson, C.; Gonzalez, F.J.; Reitman, M.L. doi  openurl
  Title Liver peroxisome proliferator-activated receptor gamma contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass Type Journal Article
  Year 2003 Publication The Journal of biological chemistry Abbreviated Journal J Biol Chem  
  Volume 278 Issue 36 Pages 34268-34276  
  Keywords Adipose Tissue/*metabolism; Animals; Blotting, Southern; Blotting, Western; Female; Hypoglycemia/genetics; Insulin Resistance/genetics; Lipid Metabolism; Liver/*metabolism; Liver Diseases/genetics/*metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; RNA/metabolism; Receptors, Cytoplasmic and Nuclear/*genetics/*physiology; Recombination, Genetic; Thiazoles/pharmacology; *Thiazolidinediones; Time Factors; Transcription Factors/*genetics/*physiology; Triglycerides/*metabolism  
  Abstract Peroxisome proliferator-activated receptor gamma (PPAR gamma) is a nuclear receptor that mediates the antidiabetic effects of thiazolidinediones. PPAR gamma is present in adipose tissue and becomes elevated in fatty livers, but the roles of specific tissues in thiazolidinedione actions are unclear. We studied the function of liver PPAR gamma in both lipoatrophic A-ZIP/F-1 (AZIP) and wild type mice. In AZIP mice, ablation of liver PPAR gamma reduced the hepatic steatosis but worsened the hyperlipidemia, triglyceride clearance, and muscle insulin resistance. Inactivation of AZIP liver PPAR gamma also abolished the hypoglycemic and hypolipidemic effects of rosiglitazone, demonstrating that, in the absence of adipose tissue, the liver is a primary and major site of thiazolidinedione action. In contrast, rosiglitazone remained effective in non-lipoatrophic mice lacking liver PPAR gamma, suggesting that adipose tissue is the major site of thiazolidinedione action in typical mice with adipose tissue. Interestingly, mice without liver PPAR gamma, but with adipose tissue, developed relative fat intolerance, increased adiposity, hyperlipidemia, and insulin resistance. Thus, liver PPAR gamma regulates triglyceride homeostasis, contributing to hepatic steatosis, but protecting other tissues from triglyceride accumulation and insulin resistance.  
  Address Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. oksanag@bdg10.niddk.nih.gov  
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  ISSN (up) 0021-9258 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:12805374 Approved no  
  Call Number refbase @ user @ Serial 81  
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Author Steiner, M. openurl 
  Title Biomechanics of tendon healing Type Journal Article
  Year 1982 Publication Journal of Biomechanics Abbreviated Journal J Biomech  
  Volume 15 Issue 12 Pages 951-958  
  Keywords Achilles Tendon/injuries; Animals; Biomechanics; Rats; Tendon Injuries/pathology/*physiopathology; Tensile Strength; Time Factors; *Wound Healing  
  Abstract The biomechanics of tendon healing was investigated with unsutured rat achilles tendons. After two, three, and four weeks of healing tensile parameters were assayed with a bone-muscle-tendon-bone preparation elongated to failure at a controlled physiological strain rate. In the third week of healing, stiffness, strength, and energy absorbing capacity all increased approximately 50%. These changes correlated with early fibroplasia. In the fourth week of healing, strength, energy absorbing capacity and elongation to failure all increased relatively more than stiffness. Histologically, larger fibers with better longitudinal alignment developed during this period. At the end of four weeks the tendon's strength was approximately 25% of normal. To summarize, the return of stiffness in a healing tendon preparation correlated with the presence of fibroplasia and the return of other tensile parameters was a function of the amount and organization of the fibroplasia.  
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  ISSN (up) 0021-9290 ISBN Medium  
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  Notes PMID:7166555 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4448  
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Author Bobbert, M.F.; Santamaria, S. doi  openurl
  Title Contribution of the forelimbs and hindlimbs of the horse to mechanical energy changes in jumping Type Journal Article
  Year 2005 Publication The Journal of Experimental Biology Abbreviated Journal J Exp Biol  
  Volume 208 Issue 2 Pages 249-260  
  Keywords Animals; Biomechanics; Forelimb/*physiology; Hindlimb/*physiology; Horses/*physiology; Locomotion/*physiology; Muscle, Skeletal/*physiology; Time Factors  
  Abstract The purpose of the present study was to gain more insight into the contribution of the forelimbs and hindlimbs of the horse to energy changes during the push-off for a jump. For this purpose, we collected kinematic data at 240 Hz from 23 5-year-old Warmbloods (average mass: 595 kg) performing free jumps over a 1.15 m high fence. From these data, we calculated the changes in mechanical energy and the changes in limb length and joint angles. The force carried by the forelimbs and the amount of energy stored was estimated from the distance between elbow and hoof, assuming that this part of the leg behaved as a linear spring. During the forelimb push, the total energy first decreased by 3.2 J kg(-1) and then increased again by 4.2 J kg(-1) to the end of the forelimb push. At the end of the forelimb push, the kinetic energy due to horizontal velocity of the centre of mass was 1.6 J kg(-1) less than at the start, while the effective energy (energy contributing to jump height) was 2.3 J kg(-1) greater. It was investigated to what extent these changes could involve passive spring-like behaviour of the forelimbs. The amount of energy stored and re-utilized in the distal tendons during the forelimb push was estimated to be on average 0.4 J kg(-1) in the trailing forelimb and 0.23 J kg(-1) in the leading forelimb. This means that a considerable amount of energy was first dissipated and subsequently regenerated by muscles, with triceps brachii probably being the most important contributor. During the hindlimb push, the muscles of the leg were primarily producing energy. The total increase in energy was 2.5 J kg(-1) and the peak power output amounted to 71 W kg(-1).  
  Address Institute for Fundamental and Clinical Human Movement Sciences, Vrije Universiteit, van der Boechorstraat 9, NL-1081 BT Amsterdam, The Netherlands. MFBobbert@fbw.vu.nl  
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  ISSN (up) 0022-0949 ISBN Medium  
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  Notes PMID:15634844 Approved no  
  Call Number Serial 1895  
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Author Witte, T.H.; Knill, K.; Wilson, A.M. doi  openurl
  Title Determination of peak vertical ground reaction force from duty factor in the horse (Equus caballus) Type Journal Article
  Year 2004 Publication The Journal of Experimental Biology Abbreviated Journal J Exp Biol  
  Volume 207 Issue Pt 21 Pages 3639-3648  
  Keywords *Acceleration; Animals; Biomechanics; Forelimb/physiology; *Gait; Hindlimb/physiology; Horses/*physiology; Locomotion/*physiology; Telemetry; Time Factors  
  Abstract Measurement of peak vertical ground reaction force (GRFz) from multiple limbs simultaneously during high-speed, over-ground locomotion would enhance our understanding of the locomotor mechanics of cursorial animals. Here, we evaluate the accuracy of predicting peak GRFz from duty factor (the proportion of the stride for which the limb is in contact with the ground). Foot-mounted uniaxial accelerometers, combined with UHF FM telemetry, are shown to be practical and accurate for the field measurement of stride timing variables, including duty factor. Direct comparison with the force plate produces a mean error of 2.3 ms and 3.5 ms for the timing of foot on and foot off, respectively, across all gaits. Predictions of peak GRFz from duty factor show mean errors (with positive values indicating an overestimate) of 0.8+/-0.04 N kg(-1) (13%; N=42; mean +/- S.E.M.) at walk, -0.3+/-0.06 N kg(-1) (3%; N=75) at trot, -2.3+/-0.27 N kg(-1) (16%; N=18) for the non-lead limb at canter and +2.1+/-0.7 N kg(-1) (19%; N=9) for the lead limb at canter. The substantial over- and underestimate seen at canter, in the lead and non-lead limbs, respectively, is attributed to the different functions performed by the two limbs in the asymmetrical gaits. The difference in load experienced by the lead and non-lead limbs decreased with increasing speed.  
  Address Structure and Motion Lab, The Royal Veterinary College, Hawkshead Lane, Hatfield, Hertfordshire, AL9 7TA, UK  
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  ISSN (up) 0022-0949 ISBN Medium  
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  Notes PMID:15371472 Approved no  
  Call Number Equine Behaviour @ team @ Serial 3658  
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Author Lagarde, J.; Kelso, J.A.S.; Peham, C.; Licka, T. openurl 
  Title Coordination dynamics of the horse-rider system Type Journal Article
  Year 2005 Publication Journal of Motor Behavior Abbreviated Journal J Mot Behav  
  Volume 37 Issue 6 Pages 418-424  
  Keywords Animals; Biomechanics; *Horses; Humans; Professional Competence; Psychomotor Performance/*physiology; *Sports; Time Factors  
  Abstract The authors studied the interaction between rider and horse by measuring their ensemble motions in a trot sequence, comparing 1 expert and 1 novice rider. Whereas the novice's movements displayed transient departures from phase synchrony, the expert's motions were continuously phase-matched with those of the horse. The tight ensemble synchrony between the expert and the horse was accompanied by an increase in the temporal regularity of the oscillations of the trunk of the horse. Observed differences between expert and novice riders indicated that phase synchronization is by no means perfect but requires extended practice. Points of contact between horse and rider may haptically convey effective communication between them.  
  Address Center for Complex Systems and Brain Sciences, Florida Atlantic University, Boca Raton, FL 33431-771, USA. lagarde@ccs.fau.edu  
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  Series Volume Series Issue Edition  
  ISSN (up) 0022-2895 ISBN Medium  
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  Notes PMID:16280312 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4034  
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Author Kirkpatrick, J.F.; Liu, I.M.; Turner, J.W.J.; Naugle, R.; Keiper, R. openurl 
  Title Long-term effects of porcine zonae pellucidae immunocontraception on ovarian function in feral horses (Equus caballus) Type Journal Article
  Year 1992 Publication Journal of reproduction and fertility Abbreviated Journal J Reprod Fertil  
  Volume 94 Issue 2 Pages 437-444  
  Keywords Animals; Contraception, Immunologic/*veterinary; *Egg Proteins; Estrogens, Conjugated (USP)/urine; Female; Glycoproteins/*pharmacology; Horses/immunology/*physiology; *Membrane Glycoproteins; Ovary/drug effects/*physiology; Progesterone/metabolism; *Receptors, Cell Surface; Swine/immunology; Time Factors; Zona Pellucida/*immunology  
  Abstract Ten feral mares free-roaming in Maryland, USA, were inoculated with porcine zonae pellucidae (PZP) protein before the breeding season for three consecutive years (1988-90). Ovarian function was monitored for 51 days during the peak of the breeding season after the third annual PZP inoculation, in seven of these mares and in four untreated control mares, by means of urinary oestrone conjugates and nonspecific progesterone metabolites. None of the ten inoculated mares became pregnant in 1990, compared with 55% of 20 control mares, which included two of the four monitored for ovarian function. Three of the untreated mares demonstrated apparent normal ovarian activity, characterized by preovulatory oestrogen peaks, concurrent progesterone nadirs at ovulation, breeding activity, and luteal-phase progesterone increases after ovulation. Two of the seven monitored PZP-treated mares demonstrated ovulatory cycles that did not result in conception. One was pregnant as a result of conception in 1989 and demonstrated a normal, late-gestation, endocrine profile. The remaining four PZP-treated mares revealed no evidence of ovulation, and urinary oestrogen concentrations were significantly depressed. The experiments indicated that (i) a third consecutive annual PZP booster inoculation is greater than 90% effective in preventing pregnancies in mares and (ii) three consecutive years of PZP treatment may interfere with normal ovarian function as shown by markedly depressed oestrogen secretion.  
  Address Deaconess Research Institute, Billings, MT 59102  
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  ISSN (up) 0022-4251 ISBN Medium  
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  Notes PMID:1317449 Approved no  
  Call Number refbase @ user @ Serial 145  
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Author Zentall, T.R. doi  openurl
  Title Support for a theory of memory for event duration must distinguish between test-trial ambiguity and actual memory loss Type Journal Article
  Year 1999 Publication Journal of the experimental analysis of behavior Abbreviated Journal J Exp Anal Behav  
  Volume 72 Issue 3 Pages 467-472  
  Keywords Animals; Behavior, Animal/physiology; Columbidae; Conditioning, Operant/physiology; Discrimination Learning/physiology; Memory/*physiology; *Psychological Theory; Time Factors; Time Perception/physiology  
  Abstract Staddon and Higa's (1999) trace-strength theory of timing and memory for event duration can account for pigeons' bias to “choose short” when retention intervals are introduced and to “choose long” when, following training with a fixed retention interval, retention intervals are shortened. However, it does not account for the failure of pigeons to choose short when the intertrial interval is distinct from the retention interval. That finding suggests that stimulus generalization (or ambiguity) between the intertrial interval and the retention interval may result in an effect that has been attributed to memory loss. Such artifacts must be eliminated before a theory of memory for event duration can be adequately tested.  
  Address Department of Psychology, University of Kentucky, Lexington 40506, USA. zentall@pop.uky.edu  
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  ISSN (up) 0022-5002 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:10605105 Approved no  
  Call Number refbase @ user @ Serial 251  
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