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Author |
Rodier, F. |
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Title |
[Spectral properties of porcine plasminogen: study of the acidic transition (author's transl)] |
Type |
Journal Article |
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Year |
1976 |
Publication |
European journal of biochemistry / FEBS |
Abbreviated Journal |
Eur J Biochem |
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Volume |
63 |
Issue |
2 |
Pages |
553-562 |
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Keywords |
Animals; Binding Sites; Guanidines; Hydrogen-Ion Concentration; *Plasminogen; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Swine; Temperature |
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Abstract |
The acidic transition of porcine plasminogen, prepared by affinity chromatography, was studied by non-destructive methods. These methods are based on the analysis of the behaviour of the tryptophyls under various conditions. The perturbation of the absorption and emission spectra by pH or temperature and the dynamic quenching of the intrinsic fluorescence are used to obtain information on structural changes which affect the environment of these residues. It is shown that by decreasing pH the fluorescence emission spectra are shifted toward the long wavelengths, with a broadening of the fluorescence band. The same effect can be obtained at constant pH by heating the protein solution. In order to analyze these phenomena, it is assumed that the fluorescence intensities at 355 nm and 328 nm reflect the proportion of the tryptophans which are exposed to the solvent, and buried, respectively. The plot of the ratio of the fluorescence intensities at these wavelengths versus pH or temperature leads to a titration curve showing an unmasking of tryptophans. The proportion of exposed tryptophans is measured by the dynamic fluorescence quenching technique and the data analyzed according to Lehrer. The plot of the fraction of exposed tryptophyls versus pH also shows the unmasking of these chromophores. Thermal perturbation of a solution of plaminogen at neutral pH induces a difference absorption spectrum whose amplitudes at the maxima are proportional to the number of exposed aromatic residues. The comparison with a solution of fully denatured plasminogen in 6 M guanidium chloride, where all the tryptophyls are exposed, shows that the percentage of exposure is equal to 59%. This number is significantly higher than the percentage found by the fluorescence quenching technique (20%), indicating that some tryptophyls are located in crevices, exposed to the solvent but not to the iodide. At acidic pH the absorption difference spectra induced by thermal perturbation are not classical, since they show an inversion and a new band between 300 nm and 305 nm. This band is mentioned in the literature as a minor band of tryptophan which appears when this chromophore is located in an asymmetric environment. On plotting the maximum amplitude of these spectra obtained at acidic pH versus temperature, we obtain a curve indicating that two types of antagonistic interactions are involved in the perturbation of the chromophores spectra. The spectrophotometric titration of plasminogen gives classical absorption difference spectra. By plotting the maximum amplitude at 292 nm versus pH, we obtain a titration curve with an apparent pK of 2.9 units. This pK is acidic which respect to the pK value of a normal carboxyl. This low value can be due to a positively charged group in the neighbourhood of a carboxyl, which interacts with one or more chromophores. When the carboxyl becomes protonated, this positively charged group is free and available to perturb the environment of some chromophores... |
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French |
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Proprietes spectrales du plasminogene porcin. Etude de la transition acide |
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0014-2956 |
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PMID:4326 |
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Call Number |
Admin @ knut @ |
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22 |
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Author |
Houpt, T.R. |
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Title |
The physiological determination of meal size in pigs |
Type |
Journal Article |
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Year |
1985 |
Publication |
The Proceedings of the Nutrition Society |
Abbreviated Journal |
Proc Nutr Soc |
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Volume |
44 |
Issue |
2 |
Pages |
323-330 |
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Keywords |
Animals; Appetite/physiology; Drinking; Duodenum/physiology; *Eating; Energy Intake; Food; Horses/physiology; Milk; Osmolar Concentration; Receptors, Cell Surface/physiology; Receptors, Cholecystokinin; Swine/*physiology; Time Factors |
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0029-6651 |
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PMID:2996010 |
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Call Number |
refbase @ user @ |
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53 |
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Author |
Houpt, K.A. |
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Title |
Animal behavior as a subject for veterinary students |
Type |
Journal Article |
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Year |
1976 |
Publication |
The Cornell veterinarian |
Abbreviated Journal |
Cornell Vet |
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Volume |
66 |
Issue |
1 |
Pages |
73-81 |
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Keywords |
Aggression; Animals; *Behavior, Animal; Cats; Chickens; Dogs; Education, Veterinary; Goats; Horses; Humans; Maternal Behavior; Mice; New York; Sexual Behavior, Animal; Sheep; Sleep; Social Behavior; Social Dominance; Swine |
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Abstract |
Knowledge of animal behavior is an important asset for the veterinarian; therefore a course in veterinary animal behavior is offered at the New York State College of Veterinary Medicine as an elective. The course emphasizes the behavior of those species of most interest to the practicing veterinarian: cats, dogs, horses, cows, pigs and sheep. Dominance heirarchies, animal communication, aggressive behavior, sexual behavior and maternal behavior are discussed. Play, learning, diurnal cycles of activity and sleep, and controls of ingestive behavior are also considered. Exotic and zoo animal behaviors are also presented by experts in these fields. The critical periods of canine development are related to the optimum management of puppies. The behavior of feral dogs and horses is described. The role of the veterinarian in preventing cruelty to animals and recognition of pain in animals is emphasized. Whenever possible behavior is observed in the laboratory or on film. |
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0010-8901 |
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Notes |
PMID:767053 |
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no |
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Call Number |
refbase @ user @ |
Serial |
61 |
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Author |
Craig, J.V. |
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Title |
Measuring social behavior: social dominance |
Type |
Journal Article |
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Year |
1986 |
Publication |
Journal of animal science |
Abbreviated Journal |
J. Anim Sci. |
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Volume |
62 |
Issue |
4 |
Pages |
1120-1129 |
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Keywords |
Aggression; Agonistic Behavior; Animals; *Behavior, Animal; Cattle; Chickens; Competitive Behavior; Female; Horses; Male; *Social Dominance; Swine |
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Abstract |
Social dominance develops more slowly when young animals are kept in intact peer groups where they need not compete for resources. Learned generalizations may cause smaller and weaker animals to accept subordinate status readily when confronted with strangers that would be formidable opponents. Sexual hormones and sensitivity to them can influence the onset of aggression and status attained. After dominance orders are established, they tend to be stable in female groups but are less so in male groups. Psychological influences can affect dominance relationships when strangers meet and social alliances within groups may affect relative status of individuals. Whether status associated with agonistic behavior is correlated with control of space and scarce resources needs to be determined for each species and each kind of resource. When such correlations exists, competitive tests and agonistic behavior associated with gaining access to scarce resources can be useful to the observer in learning about dominance relationships rapidly. Examples are given to illustrate how estimates of social dominance can be readily attained and some strengths and weaknesses of the various methods. |
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ISSN |
0021-8812 |
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Notes |
PMID:3519554 |
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no |
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Call Number |
refbase @ user @ |
Serial |
676 |
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Author |
Beaver, B.V. |
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Title |
Problems & values associated with dominance |
Type |
Journal Article |
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Year |
1981 |
Publication |
Veterinary medicine, small animal clinician : VM, SAC |
Abbreviated Journal |
Vet Med Small Anim Clin |
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Volume |
76 |
Issue |
8 |
Pages |
1129-1131 |
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Keywords |
Animals; *Animals, Domestic; *Behavior, Animal; Cats; Cattle; Dogs; Horses; *Social Dominance; Swine |
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0042-4889 |
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Notes |
PMID:6914851 |
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Call Number |
refbase @ user @ |
Serial |
678 |
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Author |
Kiley, M. |
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Title |
The vocalizations of ungulates, their causation and function |
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Journal Article |
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Year |
1972 |
Publication |
Zeitschrift für Tierpsychologie |
Abbreviated Journal |
Z. Tierpsychol. |
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Volume |
31 |
Issue |
2 |
Pages |
171-222 |
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Keywords |
Aggression; Animals; *Artiodactyla; Cattle; Fear; Female; Frustration; Horses; Humans; Male; Pain; *Perissodactyla; Sexual Behavior, Animal; Social Behavior; Social Dominance; Swine; *Vocalization, Animal |
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0044-3573 |
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PMID:4674022 |
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Call Number |
refbase @ user @ |
Serial |
681 |
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Author |
Beveridge, W.I. |
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Title |
Unravelling the ecology of influenza A virus |
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Journal Article |
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Year |
1993 |
Publication |
History and Philosophy of the Life Sciences |
Abbreviated Journal |
Hist Philos Life Sci |
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Volume |
15 |
Issue |
1 |
Pages |
23-32 |
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Keywords |
Animals; Bird Diseases/epidemiology/*history/microbiology; Birds; Ecology; History, 20th Century; Horse Diseases/epidemiology/*history/microbiology; Horses; Humans; Influenza A virus/*isolation & purification; Influenza, Human/epidemiology/*history/microbiology/*veterinary; Swine; Swine Diseases/epidemiology/*history/microbiology; Zoonoses/history |
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Abstract |
For 20 years after the influenza A virus was discovered in the early 1930s, it was believed to be almost exclusively a human virus. But in the 1950s closely related viruses were discovered in diseases of horses, pigs and birds. Subsequently influenza A viruses were found to occur frequently in many species of birds, particularly ducks, usually without causing disease. Researchers showed that human and animal strains can hybridise thus producing new strains. Such hybrids may be the cause of pandemics in man. Most pandemics have started in China or eastern Russia where many people are in intimate association with animals. This situation provides a breeding ground for new strains of influenza A virus. |
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0391-9714 |
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PMID:8310117 |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2667 |
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Author |
Alexander, D.J. |
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Title |
Ecological aspects of influenza A viruses in animals and their relationship to human influenza: a review |
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Journal Article |
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Year |
1982 |
Publication |
Journal of the Royal Society of Medicine |
Abbreviated Journal |
J R Soc Med |
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Volume |
75 |
Issue |
10 |
Pages |
799-811 |
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Keywords |
Animals; *Animals, Domestic; Bird Diseases/transmission; Birds; Disease Outbreaks; Ecology; Horse Diseases/transmission; Horses; Humans; Influenza A virus/genetics/isolation & purification; Influenza, Human/microbiology/*transmission/veterinary; Swine; Swine Diseases/transmission; Zoonoses/transmission |
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0141-0768 |
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PMID:6752410 |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2689 |
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Author |
Milouchine, V.N. |
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Title |
The role of WHO in international studies on the ecology of influenza in animals |
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Journal Article |
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Year |
1980 |
Publication |
Comparative Immunology, Microbiology and Infectious Diseases |
Abbreviated Journal |
Comp Immunol Microbiol Infect Dis |
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Volume |
3 |
Issue |
1-2 |
Pages |
25-31 |
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Keywords |
Animals; Birds/microbiology; Horses/microbiology; Humans; Influenza A virus/*isolation & purification/physiology; Orthomyxoviridae Infections/microbiology/*veterinary; Swine/microbiology; World Health Organization |
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0147-9571 |
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PMID:6258848 |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2692 |
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Author |
Iwuala, M.O.; Okpala, I. |
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Title |
Studies on the ectoparasitic fauna of Nigerian livestock II: Seasonal infestation rates |
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Journal Article |
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Year |
1978 |
Publication |
Bulletin of Animal Health and Production in Africa. Bulletin des Sante et Production Animales en Afrique |
Abbreviated Journal |
Bull Anim Health Prod Afr |
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Volume |
26 |
Issue |
4 |
Pages |
351-359 |
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Keywords |
Animal Diseases/*epidemiology; Animals; Cattle; Dogs; Ecology; Ectoparasitic Infestations/epidemiology/*veterinary; Goats; Horses; Nigeria; Seasons; Sheep; Swine |
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0378-9721 |
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PMID:756759 |
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Call Number |
Equine Behaviour @ team @ |
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2695 |
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