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Author |
Steinhoff, H.J.; Lieutenant, K.; Redhardt, A. |
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Title |
Conformational transition of aquomethemoglobin: intramolecular histidine E7 binding reaction to the heme iron in the temperature range between 220 K and 295 K as seen by EPR and temperature-jump measurements |
Type |
Journal Article |
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Year |
1989 |
Publication |
Biochimica et Biophysica Acta |
Abbreviated Journal |
Biochim Biophys Acta |
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Volume |
996 |
Issue |
1-2 |
Pages |
49-56 |
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Keywords |
Animals; Electron Spin Resonance Spectroscopy; Heme; Histidine; Horses; Humans; Hydrogen-Ion Concentration; Methemoglobin/*ultrastructure; Motion; Protein Conformation; Temperature; Thermodynamics; Water |
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Abstract |
Temperature-dependent EPR and temperature-jump measurements have been carried out, in order to examine the high-spin to low-spin transition of aquomethemogobin (pH 6.0). Relaxation rates and equilibrium constants could be determined as a function of temperature. As a reaction mechanism for the high-spin to low-spin transition, the binding of N epsilon of His E7 to the heme iron had been proposed; the same mechanism had been suggested for the ms-effect, found in temperature-jump experiments on aquomethemoglobin. A comparison of the thermodynamic quantities, deduced form the measurements in this paper, gives evidence that indeed the same reaction is investigated in both cases. Our results and most of the findings of earlier studies on the spin-state transitions of aquomethemoglobin, using susceptibility, optical, or EPR measurements, can be explained by the transition of methemoglobin with H2O as ligand (with high-spin state at all temperatures) and methemoglobin with ligand N epsilon of His E7 (with a low-spin ground state). Thermal fluctuations of large amplitude have to be postulated for the reaction to take place, so this reaction may be understood as a probe for the study of protein dynamics. |
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Institut fur Biophysik, Ruhr-Universitat Bochum, F.R.G |
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0006-3002 |
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PMID:2544230 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3803 |
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Author |
Houpt, K.A.; Zahorik, D.M.; Swartzman-Andert, J.A. |
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Title |
Taste aversion learning in horses |
Type |
Journal Article |
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Year |
1990 |
Publication |
Journal of animal science |
Abbreviated Journal |
J. Anim Sci. |
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Volume |
68 |
Issue |
8 |
Pages |
2340-2344 |
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Keywords |
Animal Feed; Animals; *Avoidance Learning; Feeding Behavior/*psychology; *Food Preferences; Horses/physiology/*psychology; *Taste |
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Abstract |
The ability of ponies to learn to avoid a relatively novel food associated with illness was tested in three situations: when illness occurred immediately after consuming a feed; when illness occurred 30 min after consuming a feed; and when illness was contingent upon eating one of three feeds offered simultaneously. Apomorphine was used to produce illness. The feeds associated with illness were corn, alfalfa pellets, sweet feed and a complete pelleted feed. The ponies learned to avoid all the fees except the complete feed when apomorphine injection immediately followed consumption of the feed. However, the ponies did not learn to avoid a feed if apomorphine was delayed 30 min after feed consumption. They could learn to avoid alfalfa pellets, but not corn, when these feeds were presented with the familiar “safe foods,” oats and soybean meal. Ponies apparently are able to learn a taste aversion, but there were constraints on this learning ability. Under the conditions of this study, they did not learn to avoid a food that made them sick long after consumption of the food, and they had more difficulty learning to avoid highly palatable feeds. |
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Cornell University, Ithaca, NY 14853 |
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0021-8812 |
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PMID:2401656 |
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no |
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Call Number |
refbase @ user @ |
Serial |
41 |
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Author |
Houpt, K.A. |
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Title |
Ingestive behavior |
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Journal Article |
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Year |
1990 |
Publication |
The Veterinary clinics of North America. Equine practice |
Abbreviated Journal |
Vet Clin North Am Equine Pract |
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Volume |
6 |
Issue |
2 |
Pages |
319-337 |
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Keywords |
Animals; Eating/*physiology; Feeding Behavior/*physiology; Horses/*physiology |
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Abstract |
In summary, horses spend 60% or more of their time eating when grazing or when feed is available free choice. Grasses are their preferred food, but they supplement the grass with herbs and woody plants. Sweetened mixtures of oats and corn are the most preferred concentrate. Horses can increase or decrease the time spent eating and amount eaten to maintain caloric intake. Their intake is stimulated by drugs such as diazepam and by the presence of other horses. Horses stop eating when gastric osmolality increases; increases in plasma osmolality, protein, and glucose accompany digestion. Foals eat several times an hour and begin sampling solid food at the same time that their dam is eating. Several areas of particular importance to the equine industry have not been investigated. These areas include the effect of exercise on short- and long-term food intake and the influence of reproductive state on the feeding of mares. |
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Department of Physiology, New York State College of Veterinary Medicine, Cornell University, Ithaca |
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ISSN |
0749-0739 |
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Notes |
PMID:2202495 |
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no |
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Call Number |
refbase @ user @ |
Serial |
42 |
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Author |
Cancedda, M. |
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Title |
[Social and behavioral organization of horses on the Giara (Sardinia): distribution and aggregation] |
Type |
Journal Article |
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Year |
1990 |
Publication |
Bollettino della Societa italiana di biologia sperimentale |
Abbreviated Journal |
Boll Soc Ital Biol Sper |
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Volume |
66 |
Issue |
11 |
Pages |
1089-1096 |
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Keywords |
Animals; *Animals, Wild/physiology/psychology; Environment; Female; *Horses/physiology/psychology; Italy; Male; Population Density; Sexual Behavior, Animal; *Social Behavior; Social Dominance; Water |
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Abstract |
In this paper some considerations on the environment of the 42 Kmq of the volcanic-basaltic Giara tableland are discussed. Conditioning by the environment and its effect on the distribution of a population of 712 horses is illustrated in view of their social and behavioural organization. |
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Istituto di Fisiologia Generale e Speciale, Universita di Sassari |
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Italian |
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Introduzione all'organizzazione sociale e comportamentale dei cavallini sulla Giara (Sardegna): distribuzione ed aggregazione |
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0037-8771 |
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Notes |
PMID:2095819 |
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no |
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Call Number |
refbase @ user @ |
Serial |
673 |
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Author |
Loyola, E.G.; Rodriguez, M.H.; Gonzalez, L.; Arredondo, J.I.; Bown, D.N.; Vaca, M.A. |
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Title |
Effect of indoor residual spraying of DDT and bendiocarb on the feeding patterns of Anopheles pseudopunctipennis in Mexico |
Type |
Journal Article |
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Year |
1990 |
Publication |
Journal of the American Mosquito Control Association |
Abbreviated Journal |
J Am Mosq Control Assoc |
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Volume |
6 |
Issue |
4 |
Pages |
635-640 |
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Keywords |
Animals; Anopheles/*physiology; *Carbamates; Cattle; *Ddt; Ecology; Enzyme-Linked Immunosorbent Assay; Feeding Behavior/*drug effects; Horses; Humans; Insect Vectors; Insecticide Resistance; *Insecticides; Mexico; *Phenylcarbamates; Seasons |
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Abstract |
Intense and persistent use of DDT for malaria control has increased resistance and induced exophilic behavior of Anopheles pseudopunctipennis. An evaluation of bendiocarb and DDT to control this species in Sinaloa, Mexico, showed that, in spite of DDT-resistance, both insecticides produced similar effects. Feeding patterns were analyzed to explain these results. Resting mosquitoes were collected over the dry and wet seasons. Anophelines were tested in an ELISA to determine the source of the meals. The human blood index (HBI) ranged from 3.3 to 6.8% in DDT- and from 12.7 to 26.9% in bendiocarb-sprayed houses. Irritability and repellency in DDT-sprayed houses could explain the reduced HBI. In contrast, bendiocarb produced higher mortality. These effects could have affected different components of the vectorial capacity and similarly reduced malaria. |
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Center for Malaria Research, Chiapas, Mexico |
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English |
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8756-971X |
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Notes |
PMID:2098469 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2671 |
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Author |
Holmstrom, M.; Magnusson, L.E.; Philipsson, J. |
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Title |
Variation in conformation of Swedish warmblood horses and conformational characteristics of elite sport horses |
Type |
Journal Article |
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Year |
1990 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
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Volume |
22 |
Issue |
3 |
Pages |
186-193 |
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Keywords |
Age Factors; Analysis of Variance; Animals; *Breeding; Female; Forelimb/anatomy & histology; Hindlimb/anatomy & histology; Horses/*anatomy & histology/physiology; Least-Squares Analysis; Male; Physical Conditioning, Animal; Regression Analysis; Sex Characteristics |
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Abstract |
The variation in conformation of 356 Swedish Warmblood horses is described, using a quantitative method of measuring horses. Thirty-three of the horses were elite dressage horses, 28 were elite showjumpers, 100 were riding school horses and 195 were unselected four-year-olds. Most horses had a long body form. The average height at the withers was 163.4 cm. Sixty per cent of the horses had a bench knee conformation, 50 per cent had a toe-in conformation of the forelimbs and 80 per cent had outwardly rotated hind limbs. The majority of these deviations were mild or moderate. Conformation was influenced by sex and age. Mares were smaller and had longer bodies and shorter limbs. The elite dressage horses and showjumpers had larger hock angles and more sloping scapulas than other horses. The showjumpers also had smaller fetlock angles in the front limbs. It is suggested that the larger hock angles among the elite horses may be because hocks with small angles are more prone to injury, and because small hock angles may negatively influence the ability to attain the degree of collection necessary for good performance in advanced classes. |
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Department of Animal Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Skara |
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0425-1644 |
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Notes |
PMID:2361507 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3756 |
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Author |
Hinrichs, K.; Watson, E.D.; Kenney, R.M. |
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Title |
Granulosa cell tumor in a mare with a functional contralateral ovary |
Type |
Journal Article |
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Year |
1990 |
Publication |
Journal of the American Veterinary Medical Association |
Abbreviated Journal |
J Am Vet Med Assoc |
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Volume |
197 |
Issue |
8 |
Pages |
1037-1038 |
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Keywords |
Animals; Corpus Luteum/*physiopathology; Female; Granulosa Cell Tumor/pathology/physiopathology/*veterinary; Horse Diseases/*pathology/physiopathology; Horses; Ovarian Neoplasms/pathology/*veterinary; Ovary/*pathology/physiopathology |
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Abstract |
A functional corpus luteum was found in the ovary contralateral to the ovary with a granulosa cell tumor in a 24-year-old Standardbred mare. The mare was ovariectomized because she was to be used as a jump mare for collection of semen from stallions. The blood concentration of progesterone was 2.2 ng/ml, and the luteal tissue progesterone concentration was 6.3 micrograms/mg. Atrophy of the contralateral ovary is one of the major signs used in diagnosis of granulosa cell tumor; however, our findings indicate that the ovary contralateral to a granulosa cell tumor is not invariably nonfunctional. |
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Department of Medicine, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536 |
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0003-1488 |
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Notes |
PMID:2243036 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3802 |
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Author |
Huizinga, H.A.; Boukamp, M.; Smolders, G. |
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Title |
Estimated parameters of field performance testing of mares from the Dutch Warmblood riding horse population |
Type |
Journal Article |
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Year |
1990 |
Publication |
Livestock Production Science |
Abbreviated Journal |
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Volume |
26 |
Issue |
4 |
Pages |
291-299 |
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Keywords |
competition; genetic parameters; horse breeding; mares; performance test |
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Abstract |
The field performance testing (FPT) of mares of the Dutch Warmblood riding horse population is evaluated. Phenotypic and genetic parameters of scored traits are estimated and the genetic relationship with performance of half-sibs in dressage and jumping competition are estimated. Data from 1984 to 1987 are used, covering scores from 2023 at least 3-year-old mares. Seven subjectively scored traits are considered, walk, trot, canter, riding ability, character, jumping ability and total score. Analysis of data is according to a sire model. Variance and covariance components are estimated by Restricted Maximum Likelihood (REML) procedures. Estimates of heritability are moderately low for gaits (average 0.19), jumping ability (0.15) and total score (0.17) and extremely low for riding ability (0.03) and character (0.06). Dressage in competition is most correlated with riding ability (0.83) and is moderately correlated with total score (0.41) from FPT of mares. Jumping competition is most correlated with jumping ability (0.48) and not correlated with total score (0.05) from field test of mares. Some possible bias owing to previous knowledge and preselection is discussed. It is concluded that efficiency of present FPT of mares is limited for selection of broodmares for dressage and jumping ability in competition. |
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Call Number |
refbase @ user @ |
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3964 |
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Permanent link to this record |
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Author |
Houpt, K.A.; Northrup, N.; Wheatley, T.; Houpt, T.R. |
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Title |
Thirst and salt appetite in horses treated with furosemide |
Type |
Journal Article |
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Year |
1991 |
Publication |
Journal of applied physiology (Bethesda, Md. : 1985) |
Abbreviated Journal |
J Appl Physiol |
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Volume |
71 |
Issue |
6 |
Pages |
2380-2386 |
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Keywords |
Animals; Appetite/*drug effects; Blood Volume; Diuresis; Drinking/drug effects; Female; Furosemide/*pharmacology; Horses; Natriuresis; Sodium, Dietary/*administration & dosage; Thirst/*drug effects |
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When a preliminary experiment in sodium-replete ponies revealed an increase, but not a significant increase, in salt consumption after furosemide treatment, the experiment was repeated using sodium-deficient horses in which aldosterone levels might be expected to be elevated to test the hypothesis that a background of aldosterone is necessary for salt appetite. Ten Standardbred mares were injected intravenously with furosemide or an equivalent volume of 0.9% sodium chloride as a control to test the effect of furosemide on their salt appetite and blood constituents. Sodium intake and sodium loss in urine, as well as water intake and urine output, were measured and compared to determine accuracy of compensation for natriuresis and diuresis. Plasma protein and packed cell volume showed significant increases in response to furosemide treatment (F = 29.31, P less than 0.001 and F = 11.20, P less than 0.001, respectively). There were no significant changes in plasma sodium concentration or osmolality in response to the treatment (P greater than 0.05). The furosemide-treated horses consumed 126 +/- 14.8 g salt, significantly more than when they were given the control injection (94.5 +/- 9.8 g; t = 2.22, P = 0.05). In response to furosemide, horses lost 962 +/- 79.7 and consumed 2,170 +/- 5 meq sodium; however, compared with control, they lost 955 meq more sodium and ingested only 570 meq more sodium, so they were undercompensating for natriuresis. The furosemide-treated horses drank 9.6 +/- 0.8 kg of water, significantly more than when they received the control injection (6.4 +/- 0.8 kg; t = 6.9, P less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Department of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401 |
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8750-7587 |
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Notes |
PMID:1778936 |
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no |
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Call Number |
refbase @ user @ |
Serial |
38 |
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Permanent link to this record |
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Author |
Houpt, K.A. |
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Title |
Animal behavior and animal welfare |
Type |
Journal Article |
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Year |
1991 |
Publication |
Journal of the American Veterinary Medical Association |
Abbreviated Journal |
J Am Vet Med Assoc |
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Volume |
198 |
Issue |
8 |
Pages |
1355-1360 |
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Keywords |
*Animal Welfare; Animals; *Behavior, Animal; Cats/*psychology; Hoof and Claw/surgery; Horses/*psychology; Housing, Animal |
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Abstract |
The value of behavioral techniques in assessing animal welfare, and in particular assessing the psychological well being of animals, is reviewed. Using cats and horses as examples, 3 behavioral methods are presented: (1) comparison of behavior patterns and time budgets; (2) choice tests; and (3) operant conditioning. The behaviors of intact and declawed cats were compared in order to determine if declawing led to behavioral problems or to a change in personality. Apparently it did not. The behavior of free ranging horses was compared with that of stabled horses. Using two-choice preference tests, the preference of horses for visual contact with other horses and the preference for bedding were determined. Horses show no significant preference for locations from which they can make visual contact with other horses, but they do prefer bedding, especially when lying down. Horses will perform an operant response in order to obtain light in a darkened barn or heat in an outside shed. These same techniques can be used to answer a variety of questions about an animal's motivation for a particular attribute of its environment. |
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New York State College of Veterinary Medicine, Cornell University, Ithaca 14853 |
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0003-1488 |
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Notes |
PMID:2061151 |
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no |
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Call Number |
refbase @ user @ |
Serial |
40 |
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Permanent link to this record |