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Mizuguchi, M.; Arai, M.; Ke, Y.; Nitta, K.; Kuwajima, K. |
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Title |
Equilibrium and kinetics of the folding of equine lysozyme studied by circular dichroism spectroscopy |
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Journal Article |
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Year |
1998 |
Publication  |
Journal of Molecular Biology |
Abbreviated Journal |
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283 |
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1 |
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265-277 |
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Keywords |
equine lysozyme; protein folding; molten globule; stopped-flow; folding intermediate |
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Abstract |
The equilibrium unfolding and the kinetics of unfolding and refolding of equine lysozyme, a Ca2+-binding protein, were studied by means of circular dichroism spectra in the far and near-ultraviolet regions. The transition curves of the guanidine hydrochloride-induced unfolding measured at 230 nm and 292.5 nm, and for the apo and holo forms of the protein have shown that the unfolding is well represented by a three-state mechanism in which the molten globule state is populated as a stable intermediate. The molten globule state of this protein is more stable and more native-like than that of α-lactalbumin, a homologous protein of equine lysozyme. The kinetic unfolding and refolding of the protein were induced by concentration jumps of the denaturant and measured by stopped-flow circular dichroism. The observed unfolding and refolding curves both agreed well with a single-exponential function. However, in the kinetic refolding reactions below 3 M guanidine hydrochloride, a burst-phase change in the circular dichroism was present, and the burst-phase intermediate in the kinetic refolding is shown to be identical with the molten globule state observed in the equilibrium unfolding. Under a strongly native condition, virtually all the molecules of equine lysozyme transform the structure from the unfolded state into the molten globule, and the subsequent refolding takes place from the molten globule state. The transition state of folding, which may exist between the molten globule and the native states, was characterized by investigating the guanidine hydrochloride concentration-dependence of the rate constants of refolding and unfolding. More than 80% of the hydrophobic surface of the protein is buried in the transition state, so that it is much closer to the native state than to the molten globule in which only 36% of the surface is buried in the interior of the molecule. It is concluded that all the present results are best explained by a sequential model of protein folding, in which the molten globule state is an obligatory folding intermediate on the pathway of folding. |
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refbase @ user @ |
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3990 |
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Author |
Burgon, H.L. |
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Title |
‘Queen of the world’: experiences of ‘at-risk’ young people participating in equine-assisted learning/therapy |
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Journal Article |
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Year |
2011 |
Publication |
Journal of Social Work Practice |
Abbreviated Journal |
Journal of Social Work Practice |
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Volume |
25 |
Issue |
02 |
Pages |
165-183 |
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There is a significant body of research into the benefits of animal-assisted therapy (AAT) but less into the fields known as equine-assisted learning and therapy (EAL/EAT) where horses are incorporated in therapeutic and learning interventions. This paper explores the experiences of seven ?at-risk? young people who participated in a therapeutic horsemanship (TH) programme. The study followed a practice-near approach seeking to capture the young people's experiences within a participative ethnography. Themes related to the risk and resilience literature such as self-confidence, self-esteem, self-efficacy and a sense of mastery, empathy and the opening of positive opportunities are explored in this paper. |
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0265-0533 |
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doi: 10.1080/02650533.2011.561304 |
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Call Number |
Equine Behaviour @ team @ |
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6032 |
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Author |
Crans, W.J.; McNelly, J.; Schulze, T.L.; Main, A. |
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Title |
Isolation of eastern equine encephalitis virus from Aedes sollicitans during an epizootic in southern New Jersey |
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Journal Article |
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Year |
1986 |
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Journal of the American Mosquito Control Association |
Abbreviated Journal |
J Am Mosq Control Assoc |
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Volume |
2 |
Issue |
1 |
Pages |
68-72 |
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Keywords |
Aedes/*microbiology; Alphavirus/*isolation & purification; Animals; Disease Outbreaks/veterinary; Ecology; Encephalitis Virus, Eastern Equine/*isolation & purification; Encephalomyelitis, Equine/epidemiology/transmission/veterinary; Horse Diseases/epidemiology/transmission; Horses; Insect Vectors/microbiology; New Jersey |
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Abstract |
Eastern equine encephalitis virus (EEE) was isolated from the salt marsh mosquito, Aedes sollicitans, collected from coastal areas of New Jersey on 3 occasions during the late summer and fall of 1982. The isolations were made at a time when local Culiseta melanura were either undergoing a population increase or exhibiting high levels of EEE virus. Although no human cases were reported during the epizootic period, the data lend support to the hypothesis that Ae. sollicitans is capable of functioning as an epidemic vector in the coastal areas of New Jersey where human cases of EEE have been most common. |
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Mosquito Research and Control, Cook College, Rutgers University, New Brunswick, NJ 08903 |
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8756-971X |
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PMID:2853203 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2684 |
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Author |
Mitchell, C.J.; Darsie, R.F.J.; Monath, T.P.; Sabattini, M.S.; Daffner, J. |
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Title |
The use of an animal-baited net trap for collecting mosquitoes during western equine encephalitis investigations in Argentina |
Type |
Journal Article |
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Year |
1985 |
Publication |
Journal of the American Mosquito Control Association |
Abbreviated Journal |
J Am Mosq Control Assoc |
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Volume |
1 |
Issue |
1 |
Pages |
43-47 |
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Keywords |
Animals; Argentina; *Culicidae/classification; Disease Outbreaks/veterinary; Encephalitis Virus, Western Equine; Encephalomyelitis, Equine/transmission/*veterinary; Entomology/*instrumentation; Equipment Design; Horse Diseases/*transmission; Horses; *Insect Vectors; Population Surveillance |
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A large net trap was used to sample mosquito populations attracted to horses at three sites each in Santa Fe and Rio Negro Provinces, Argentina, during the austral summer of 1984. These provinces, as well as others in Argentina, were affected by a severe epizootic of western equine encephalitis (WEE) during 1982-83. Totals of 2,752 and 6,929 mosquitoes were collected in Santa Fe and Rio Negro Provinces during five and three trap nights, respectively. Culex mosquitoes of the subgenus Culex were predominant (45.8% of total) in the Santa Fe collections, although Aedes albifasciatus also was prevalent (21.7%). The latter species was predominant (95.7% of total) in the Rio Negro collections. The mosquito fauna was less complex (minimum of 6 species) in Rio Negro Province as compared to Santa Fe Province (minimum of 18 species). The advantages of the net trap indicate that this trap can become a useful tool in arbovirus ecology studies in other areas. |
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Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, CO 80522-2087 |
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8756-971X |
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Notes |
PMID:2906656 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2686 |
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Author |
Holbrook, A.A. |
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Title |
Biology of equine piroplasmosis |
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Journal Article |
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Year |
1969 |
Publication |
Journal of the American Veterinary Medical Association |
Abbreviated Journal |
J Am Vet Med Assoc |
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155 |
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2 |
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453-454 |
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Animals; Arthropod Vectors; Babesiosis/*epidemiology; Ecology; Epidemiology; Florida; Horse Diseases/*epidemiology; Horses; Ticks |
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0003-1488 |
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PMID:5816130 |
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no |
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Equine Behaviour @ team @ |
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2740 |
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Madigan, J.E.; Whittemore, J. |
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The role of the equine practitioner in disasters |
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Journal Article |
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Year |
2000 |
Publication |
Journal of the American Veterinary Medical Association |
Abbreviated Journal |
J Am Vet Med Assoc |
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216 |
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8 |
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1238-1239 |
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*Animal Husbandry/education; *Animal Welfare; Animals; Disaster Planning; *Horses; *Natural Disasters; United States; *Veterinary Medicine |
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Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis 95616, USA |
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0003-1488 |
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PMID:10767959 |
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no |
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Equine Behaviour @ team @ |
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4055 |
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Author |
Sinclair, M.; Buhrmann, G.; Gummow, B. |
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Title |
An epidemiological investigation of the African horsesickness outbreak in the Western Cape Province of South Africa in 2004 and its relevance to the current equine export protocol |
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Journal Article |
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Year |
2006 |
Publication |
Journal of the South African Veterinary Association |
Abbreviated Journal |
J S Afr Vet Assoc |
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77 |
Issue |
4 |
Pages |
191-196 |
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African Horse Sickness/diagnosis/*epidemiology; African horse sickness virus/*isolation & purification; Animals; Ceratopogonidae/virology; Diagnosis, Differential; Disease Outbreaks/*veterinary; Female; Horses; Insect Vectors/virology; Male; Prevalence; Retrospective Studies; Sentinel Surveillance; South Africa/epidemiology; Viral Vaccines/administration & dosage |
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African Horsesickness (AHS) is a controlled disease in South Africa. The country is divided into an infected area and a control area. An outbreak of AHS in the control area can result in a ban of exports for at least 2 years. A retrospective epidemiological study was carried out on data collected during the 2004 AHS outbreak in the surveillance zone of the AHS control area in the Western Cape Province. The objective of this study was to describe the 2004 outbreak and compare it with the 1999 AHS outbreak in the same area. As part of the investigation, a questionnaire survey was conducted in the 30 km radius surrounding the index case. Spatial, temporal and population patterns for the outbreak are described. The investigation found that the outbreak occurred before any significant rainfall and that the main AHS vector (Culicoides imicola) was present in abundance during the outbreak. Furthermore, 63% of cases occurred at temperatures < or = 15 degrees C, the Eerste River Valley was a high risk area, only 17% of owners used vector protection as a control measure and 70% of horses in the outbreak area were protected by means of vaccination at the start of the outbreak. The study revealed that the current AHS control measures do not function optimally because of the high percentage of vaccinated horses in the surveillance zone, which results in insufficient sentinel animals and the consequent failure of the early warning system. Alternative options for control that allow continued export are discussed in the paper. |
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State Veterinarian Epidemiology, Elsenburg, South Africa. marnas@elsenburg.com |
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1019-9128 |
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PMID:17458343 |
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Equine Behaviour @ team @ |
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2354 |
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Fenner, K.; Freire, R.; McLean, A.; McGreevy, P. |
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Behavioral, demographic and management influences on equine responses to negative reinforcement |
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Journal Article |
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2018 |
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Journal of Veterinary Behavior |
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Learning; horse management; training; temperament; negative reinforcement |
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Understanding the factors that influence horse learning is critical to ensure horse welfare and rider safety. In this study, data were obtained from horses (n=96) training to step backwards through a corridor in response to bit pressure. Following training, learning ability was determined by the latency to step backwards through the corridor when handled on the left and right reins. Additionally, horse owners were questioned about each horse's management, training, behavior and signalment (such as horse breed, age and sex). Factors from these four broad domains were examined using a multiple logistic regression (MLR) model, following an Information Theoretic approach, for associations between horses' behavioral attributes and their ability to learn the task. The MLR also included estimates of the rider's ability and experience as well as owner's perceptions of their horse's trainability and temperament. Results revealed several variables including explanatory variables that correlated significantly with rate of learning. Horses were faster at backing, a behavioral trait, when handled on the right (t = 3.65, df = 94, P < 0.001) than the left side. Thoroughbred horses were slower at completing the tests than other breeds of horses when handled on the left side (LM, F1,48=4.5, P=0.04) and right side (LM, F1,45=6.0, P=0.02). Those in regular work, a training factor, did not learn faster than their unworked counterparts on the right rein but completed the task faster on the left rein (F1,44=5.47, P=0.02). This may reflect differences in laterality and habituation effects. In contrast, more anxious horses were faster at completing the test when handled from the right (Spearman, r=-0.22, P=0.04). It is possible that these horses have an increased arousal level when interacting with handlers, resulting in more engagement with the lesson, accounting for the improved performance results. The findings of this study will help clarify how horse behavior, training and management may influence learning and how their application may optimize learning outcomes. Future equine behavior assessment and research questionnaires should include items that assess these qualities. |
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1558-7878 |
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Equine Behaviour @ team @ |
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6400 |
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Author |
Roels, S.; Tilmant, K.; Van Daele, A.; Van Marck, E.; Ducatelle, R. |
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Proliferation, DNA ploidy, p53 overexpression and nuclear DNA fragmentation in six equine melanocytic tumours |
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Journal Article |
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2000 |
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Journal of Veterinary Medicine, Series A |
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47 |
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439-438 |
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refbase @ user @ |
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2016 |
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Author |
Passler, S.; Pfeffer, M. |
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Title |
Detection of antibodies to alphaviruses and discrimination between antibodies to eastern and western equine encephalitis viruses in rabbit sera using a recombinant antigen and virus-specific monoclonal antibodies |
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Journal Article |
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Year |
2003 |
Publication |
Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health |
Abbreviated Journal |
J Vet Med B Infect Dis Vet Public Health |
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50 |
Issue |
6 |
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265-269 |
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Animals; Antibodies, Monoclonal/*immunology; Antibodies, Viral/*analysis/blood; DNA Primers; Encephalitis Virus, Eastern Equine/genetics/*immunology; Encephalitis Virus, Western Equine/genetics/*immunology; Encephalomyelitis, Equine/*diagnosis/*virology; Epitopes; Fluorescent Antibody Technique/*veterinary; Horses; Rabbits; Recombination, Genetic; Reverse Transcriptase Polymerase Chain Reaction/veterinary |
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Three arthropod-borne alphaviruses, western equine encephalitis viruses (WEEV), eastern equine encephalitis viruses (EEEV) and Venezuelan equine encephalitis viruses are the aetiological agents of a sometimes severe encephalomyelitis in equines and humans in the New World. With regard to the different ecology and epidemiology of these viruses, a method applied in serological screening should be able to distinguish between them as well as other related members of the genus Alphavirus in the American continent. However, this has been hampered in the past by (a) the close antigenic relationship between alphaviruses in traditional serological assays, especially in the routinely used haemagglutination-inhibition, and (b) the need of biosafety level 3 facilities to grow the viral antigens. An epitope blocking assay using an EEEV glycoprotein E1-expressing recombinant Sindbis virus and virus-specific monoclonal antibodies (mAbs) binding to the E1 of EEEV (strain NJ/60) and the E1 of Sindbis virus was established using automated flow cytometry. The test was evaluated using sera of infected and vaccinated rabbits. A cut-off value of 30% inhibition for antigenic complex-specific seroconversion was found to be sufficient for the detection of the respective infection. By using three different mAbs in parallel, we were able to detect alphavirus genus-, EEEV- and WEEV-complex-specific serum antibodies. As this test is based on the inhibition of binding of virus-specific mAbs, sera of every origin other than mouse can be tested. Thus, this assay may prove useful in the serological screening of a variety of animal species during an outbreak investigation. |
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Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany |
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0931-1793 |
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Notes |
PMID:14628996 |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2639 |
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