| |
Records |
Links |
|
Author |
Tiefenbacher, S.; Lee, B.; Meyer, J.S.; Spealman, R.D. |
|
| |
Title |
Noninvasive technique for the repeated sampling of salivary free cortisol in awake, unrestrained squirrel monkeys |
Type |
Journal Article |
| |
Year |
2003 |
Publication |
American Journal of Primatology |
Abbreviated Journal |
Am. J. Primatol. |
|
| |
Volume |
60 |
Issue  |
2 |
Pages |
69-75 |
|
| |
Keywords |
saliva; cortisol; squirrel monkey; sampling technique; Hpa |
|
| |
Abstract |
The use of noninvasive measures of hypothalamic-pituitary-adrenal (HPA) axis function is of growing interest among preclinical and clinical investigators. This report describes a method for the repeated assessment of salivary free cortisol in awake, unrestrained squirrel monkeys (Saimiri sciureus) based on a saliva sampling technique previously developed for rhesus monkeys. Individually housed adult male squirrel monkeys were trained to chew on dental rope attached to a pole, from which saliva was extracted by centrifugation and analyzed for cortisol by radioimmunoassay (RIA). Eight of nine monkeys readily acquired the task, reliably providing adequate saliva samples for the assay. Salivary free cortisol levels were examined in these subjects under basal conditions and in response to two types of neuroendocrine challenge. Levels of salivary free cortisol showed relatively low intra- and interindividual variability, with mean individual morning levels ranging between 17.1 and 37.9 µg/dl. Squirrel monkeys demonstrated a consistent daily rhythm in salivary free cortisol ranging from a high of 27.4 ± 5.2 µg/dl (mean ± SEM) at 12 P.M. to a low of 7.5 ± 1.6 µg/dl at 6 P.M.. Intravenous (IV) challenges with 1 µg/kg ACTH, or 10 and 50 µg/kg CRF resulted in significant increases in salivary free cortisol. The described sampling technique provides a reliable and sensitive means for repeated measurement of HPA activity in unrestrained, awake squirrel monkeys. In addition, our findings illustrate several features of HPA system rhythmicity and reactivity using salivary cortisol instead of blood plasma or serum. Am. J. Primatol. 60:69–75, 2003. © 2003 Wiley-Liss, Inc. |
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Wiley Subscription Services, Inc., A Wiley Company |
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
1098-2345 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
|
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
5573 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Alexander, F. |
|
| |
Title |
A study of parotid salivation in the horse |
Type |
Journal Article |
| |
Year |
1966 |
Publication |
The Journal of physiology |
Abbreviated Journal |
J Physiol |
|
| |
Volume |
184 |
Issue |
3 |
Pages |
646-656 |
|
| |
Keywords |
Animals; Atropine/*pharmacology; Bicarbonates/metabolism; Calcium/metabolism; Chlorides/metabolism; Horses; Mastication/*physiology; Parotid Gland/*physiology; Pilocarpine/*pharmacology; Potassium/metabolism; Salivation/*drug effects; Sodium/metabolism; Tetracaine/*pharmacology |
|
| |
Abstract |
|
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0022-3751 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:5963737 |
Approved |
no |
|
| |
Call Number |
refbase @ user @ |
Serial |
119 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Shanahan, S. |
|
| |
Title |
Trailer loading stress in horses: behavioral and physiological effects of nonaversive training (TTEAM) |
Type |
Journal Article |
| |
Year |
2003 |
Publication |
Journal of Applied Animal Welfare Science : JAAWS |
Abbreviated Journal |
J Appl Anim Welf Sci |
|
| |
Volume |
6 |
Issue |
4 |
Pages |
263-274 |
|
| |
Keywords |
Animals; *Conditioning, Operant; *Escape Reaction; Female; Heart Rate; Horses/*psychology; Hydrocortisone/metabolism; Male; Saliva/metabolism; Stress/metabolism/prevention & control/*veterinary; *Transportation |
|
| |
Abstract |
Resistance in the horse to trailer loading is a common source of stress and injury to horses and their handlers. The objective of this study was to determine whether nonaversive training based on the Tellington-Touch Equine Awareness Method (TTEAM; Tellington-Jones &Bruns, 1988) would decrease loading time and reduce stress during loading for horses with a history of reluctance to load. Ten horses described by their owners as “problem loaders” were subjected to pretraining and posttraining assessments of loading. Each assessment involved two 7-min loading attempts during which heart rate and saliva cortisol were measured. The training consisted of six 30-min sessions over a 2-week period during which the horse and owner participated in basic leading exercises with obstacles simulating aspects of trailering. Assessment showed heart rate and saliva cortisol increased significantly during loading as compared to baseline (p <.001 and p <.05, respectively). Reassessment after training showed a decrease in loading time (p <.02), reduced heart rate during loading (p <.002), and reduced saliva cortisol as compared to pretraining assessments. Seven “good loaders” also were subject to loading assessment for physiological comparison. Increases in heart rate during loading were significantly higher in the good loaders (p <.001). Nonaversive training simulating aspects of loading may effectively reduce loading time and stress during loading for horses with a history of resistance to trailer loading. |
|
| |
Address |
shanahandvm@yahoo.ca |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
1088-8705 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:14965781 |
Approved |
no |
|
| |
Call Number |
|
Serial |
1903 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Peeters, M.; Sulon, J.; Beckers, J.-F.; Ledoux, D.; Vandenheede, M. |
|
| |
Title |
Comparison between blood serum and salivary cortisol concentrations in horses using an adrenocorticotropic hormone challenge |
Type |
Journal Article |
| |
Year |
2011 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
|
|
| |
Volume |
43 |
Issue |
4 |
Pages |
487-493 |
|
| |
Keywords |
horse; cortisol; ACTH challenge; saliva; stress |
|
| |
Abstract |
Reasons for performing study: In horses, serum cortisol concentration is considered to provide an indirect measurement of stress. However, it includes both free and bound fractions. The sampling method is also invasive and often stressful. This is not the case for salivary cortisol, which is collected using a more welfare-friendly method and represents a part of the free cortisol fraction, which is the biologically active form. Objectives: To compare salivary and serum cortisol assays in horses, in a wide range of concentrations, using an adrenocorticotropic hormone (ACTH) stimulation test, in order to validate salivary cortisol for stress assessment in horse. Methods: In 5 horses, blood samples were drawn using an i.v. catheter. Saliva samples were taken using swabs. Cortisol was assayed by radioimmunoassay. All data were treated with a regression method, which pools and analyses data from multiple subjects for linear analysis. Results: Mean ± s.d. cortisol concentrations measured at rest were 188.81 ± 51.46 nmol/l in serum and 1.19 ± 0.54 nmol/l in saliva. They started increasing immediately after ACTH injection and peaks were reached after 96 ± 16.7 min in serum (356.98 ± 55.29 nmol/l) and after 124 ± 8.9 min in saliva (21.79 ± 7.74 nmol/l, P<0.05). Discharge percentages were also different (225% in serum and 2150% in saliva, P<0.05). Correlation between serum and salivary cortisol concentrations showed an adjusted r2= 0.80 (P<0.001). The strong link between serum and salivary cortisol concentrations was also estimated by a regression analysis. Conclusions: The reliability of both RIAs and regression found between serum and salivary cortisol concentrations permits the validation of saliva-sampling as a noninvasive technique for cortisol level assessment in horses. |
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Blackwell Publishing Ltd |
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
2042-3306 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
|
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
5428 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Skandakumar, S.; Stodulski, G.; Hau, J. |
|
| |
Title |
Salivary IgA: a Possible Stress Marker In Dogs |
Type |
Abstract |
| |
Year |
1995 |
Publication |
Animal Welfare |
Abbreviated Journal |
|
|
| |
Volume |
4 |
Issue |
4 |
Pages |
339-350 |
|
| |
Keywords |
Animal Welfare; Behaviour; Cortisol; Dog; Salivary Iga (S-Iga); Stress; Well-Being |
|
| |
Abstract |
Stress in humans has been reported to be associated with a decrease in the salivary immunoglobulin A (s-IgA) levels enabling the possible use of s-IgA to assess stress. Prolonged stress, if reliably assessed in a non-invasive manner, may be used to assess animal welfare. This study analysed groups of dogs undergoing physical and temperamental training and s-IgA levels were measured by rocket immunoelectrophoresis in prospective samples. Behavioural assessment was carried out and cortisol levels in saliva were measured by ELISA. A significant negative correlation (P < 0.007) between the logarithmic cortisol concentrations and s-IgA levels in saliva was recorded. The behavioural assessment of the dogs agreed well with the biochemical markers. It is concluded that IgA levels in saliva may be a useful marker of dog well-being and that stress results in decreased s-IgA levels. |
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
|
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
5964 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Alexander, F.; Horner, M.W.; Moss, M.S. |
|
| |
Title |
The salivary secretion and clearance in the horse of chloral hydrate and its metabolites |
Type |
Journal Article |
| |
Year |
1967 |
Publication |
Biochemical pharmacology |
Abbreviated Journal |
Biochem Pharmacol |
|
| |
Volume |
16 |
Issue |
7 |
Pages |
1305-1311 |
|
| |
Keywords |
Animals; Chloral Hydrate/blood/*metabolism/urine; Chromatography, Gas; Ethanol/blood/urine; Horses; Male; Parotid Gland/metabolism; Saliva/*analysis; Trichloroacetic Acid/blood/urine |
|
| |
Abstract |
|
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0006-2952 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:6053598 |
Approved |
no |
|
| |
Call Number |
refbase @ user @ |
Serial |
118 |
|
| Permanent link to this record |
