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	Author	Mejdell, C.M.; Simensen, E.; Boe, K.E.
	Title	Is snow a sufficient source of water for horses kept outdoors in winter? A case report			Type	Journal Article
	Year	2005	Publication	Acta Veterinaria Scandinavica	Abbreviated Journal	Acta Vet Scand
	Volume	46	Issue	1-2	Pages	19-22
	Keywords	Animals; Drinking Behavior; Horses; Norway; Osmolar Concentration; Snow; Thirst; Water Supply
	Abstract	Due to extreme weather conditions, a flock of outwintered Icelandic horses had to manage for several days on snow as the source of free water. They were fed grass silage ad lib, and any change in feed consumption was not observed. After nine days, blood samples were taken and analysed for plasma osmolality, they were subjected to a simple clinical examination, and offered drinking water. Osmolality levels were within normal limits and mean value did not differ significantly from samples which previously were taken of the same individuals. The general condition of the horses was normal, with no signs of clinical dehydration or disease. The horses showed very little interest for the offered drinking water. This suggests that in cold winter weather, horses being fed grass silage and adjusted to eat snow, can manage for several days with snow substituting liquid water without their physiology and welfare being challenged.
	Address	National Centre for Veterinary Contract Research and Commercial Services, Ltd
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0044-605X	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:16108209			Approved	no
	Call Number				Serial	1886
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	Author	Holzapfel, W.H.; Botha, S.J.
	Title	Physiology of Sporolactobacillus strains isolated from different habitats and the indication of in vitro antagonism against Bacillus species			Type	Journal Article
	Year	1988	Publication	International Journal of Food Microbiology	Abbreviated Journal	Int J Food Microbiol
	Volume	7	Issue	2	Pages	161-168
	Keywords	Animals; Bacillaceae/isolation & purification/physiology; Bacillus/physiology; Cattle; Ecology; Feces/microbiology; Food Microbiology; Horses; Sewage; Sheep; Water Microbiology
	Abstract	In an ecological study only low numbers of Sporolactobacillus were found in habitats such as the faeces of herbivores, the rumen of cattle and the final waste water of an abattoir. Their presence in the final waste water of an abattoir indicates their possible association with food, and, more specifically, with meat. Differences were found in some physiological characteristics. One isolate (L2404) differed from the authentic Sporolactobacillus ATCC 15538 by its inability to ferment inulin, its growth in presence of 6.5% NaCl and in 0.2% tellurite, by the isomer(s) of lactic acid produced and the mol% G + G in the DNA. One Sporolactobacillus isolate (L2407) showed antagonism against Bacillus cereus, Bacillus cereus var, mycoides, Bacillus megaterium and Bacillus subtilis.
	Address	Department of Microbiology and Plant Pathology, University of Pretoria, Republic of South Africa
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0168-1605	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:3275317			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	2675
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	Author	Beerwerth, W.; Schurmann, J.
	Title	[Contribution to the ecology of mycobacteria]			Type	Journal Article
	Year	1969	Publication	Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie, Virusforschung und Parasitologie. Originale	Abbreviated Journal	Zentralbl Bakteriol [Orig]
	Volume	211	Issue	1	Pages	58-69
	Keywords	Animal Feed; Animals; Cattle; Chickens; Ecology; Feces/microbiology; Food Microbiology; Germany, West; Horses; Hydroxides; Mycobacterium/classification/isolation & purification; Mycobacterium tuberculosis/isolation & purification; Oxalates; Sewage; Sheep; Sodium; Soil Microbiology; Swine; *Water Microbiology
	Abstract
	Address
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	German	Summary Language		Original Title	Zur Okologie der Mykobakterien
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0372-8110	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:4989344			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	2743
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	Author	Domjan, M.
	Title	Determinants of the enhancement of flavored-water intake by prior exposure			Type	Journal Article
	Year	1976	Publication	Journal of Experimental Psychology. Animal Behavior Processes	Abbreviated Journal	J Exp Psychol Anim Behav Process
	Volume	2	Issue	1	Pages	17-27
	Keywords	Animals; Arousal; Awareness; Behavior, Animal; Cognition; Drinking Behavior; Environment; Escape Reaction; Fear; Male; Rats; Saccharin/administration & dosage; Taste; Thirst; Time Factors; Water Deprivation
	Abstract	The intake of a 2.0% sodium saccharin solution in rats was observed to increase as a function of both the number (Experiment 1) and the duration (Experiment 3) of prior periods of access to the saccharin flavor, but did not increase when subjects were maintained on a fluid deprivation procedure in the absence of saccharin exposure (Experiment 2). The enhancement of intake was further influenced by the schedule of saccharin preexposures in the absence of variations in the amount of solution tasted (Experiment 4). The effect was not a function of the opportunity for subjects to determine their own pattern of contact with the saccharin flavor, the opportunity for association of the flavor with hunger and thirst reduction, or the amount of saccharin swallowed during preexposure (Experiment 5). These results suggest that mere exposure to a flavored solution is sufficient to increase subsequent intakes. The phenomenon is discussed in terms of the attenuation of neophobia elicited by the novelty of flavored solutions.
	Address
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0097-7403	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:1249524			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	2790
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	Author	Hinchcliff, K.W.; Kohn, C.W.; Geor, R.; McCutcheon, L.J.; Foreman, J.; Andrews, F.M.; Allen, A.K.; White, S.L.; Williamson, L.H.; Maykuth, P.L.
	Title	Acid:base and serum biochemistry changes in horses competing at a modified 1 Star 3-day-event			Type	Journal Article
	Year	1995	Publication	Equine Veterinary Journal. Supplement	Abbreviated Journal	Equine Vet J Suppl
	Volume		Issue	20	Pages	105-110
	Keywords	Acid-Base Equilibrium; Animals; Blood Proteins/analysis; Body Water/metabolism; Carbon Dioxide/blood; Electrolytes/blood; Female; Hematocrit/veterinary; Homeostasis; Horses/blood/physiology; Hydrogen-Ion Concentration; Male; Physical Conditioning, Animal/physiology
	Abstract	We examined the effects of participation in each of 3 modifications of Day 2 of a 3-day-event on blood and serum variables indicative of hydration, acid:base status and electrolyte homeostasis of horses. Three groups of horses – 8 European (E) horses and 2 groups each of 9 North American horses performed identical Days 1 (dressage) and 3 (stadium jumping) of a 3-day-event. E horses and one group of the North American horses (TD) performed modifications of Day 2 of a 1 Star 3-day-event and the other group of North American horses (HT) performed a Horse Trial on Day 2. Jugular venous blood was collected from each horse on the morning of Day 2 before any warm-up activity, between 4 min 55 s and 5 min 15 s after Phase D and the following morning. Eight E horses, 5 TD horses and 8 HT horses completed the trials. There were few significant differences in acid:base or serum biochemistry variables detected among horses performing either 2 variations of the Speed and Endurance day of a 1 Star 3-day-event, or a conventional Horse Trial. Failure to detect differences among groups may have been related to the low statistical power associated with the small number of horses, especially in the TD group, variation in quality of horses among groups and the different times of the day at which the E horses competed. Differences detected among time points were usually common to all groups and demonstrated metabolic acidosis with a compensatory respiratory alkalosis, a reduction in total body water and cation content, and hypocalcaemia. Importantly, horses of all groups did not replenish cation, chloride, and calcium deficits after 14-18 h of recovery.
	Address	Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210-1089, USA
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN		ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:8933092			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	3740
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	Author	Andrews, F.M.; Ralston, S.L.; Sommardahl, C.S.; Maykuth, P.L.; Green, E.M.; White, S.L.; Williamson, L.H.; Holmes, C.A.; Geiser, D.R.
	Title	Weight, water, and cation losses in horses competing in a three-day event			Type	Journal Article
	Year	1994	Publication	Journal of the American Veterinary Medical Association	Abbreviated Journal	J Am Vet Med Assoc
	Volume	205	Issue	5	Pages	721-724
	Keywords	Animals; Body Water/metabolism; Body Weight/physiology; Exertion/physiology; Female; Horses/blood/metabolism; Male; Physical Conditioning, Animal/physiology; Physical Endurance/physiology; Potassium/blood; Sodium/blood
	Abstract	Body weight of 48 horses competing in a 3-day event was measured the day before the event (baseline), following the dressage phase of the event (day 1), after the endurance phases of the event (day 2), and 18 to 24 hours after the endurance phases (day 3). Plasma sodium and potassium concentrations were measured the evening before, immediately after, and 10 minutes after the endurance phases. Total body water, water loss, and net exchangeable cation loss were then calculated. Body weight and total body water were significantly decreased, compared with baseline values, at all times during the event, and significant water loss was detected. The largest changes were recorded after the endurance phases of the event. Water deficits were still detected 18 to 24 hours after the endurance phases of the event. Mean plasma sodium concentration was significantly increased immediately after the endurance phases of the event, compared with concentration measured the evening before, and remained increased after the 10-minute recovery period, presumably because of dehydration. Mean plasma potassium concentration was significantly increased immediately after the endurance phases of the event, compared with concentration measured the evening before, but was not increased after the 10-minute recovery period.
	Address	Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0003-1488	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:7989242			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	3745
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	Author	Sloet van Oldruitenborgh-Oosterbaan, M.M.; Spierenburg, A.J.; van den Broek, E.T.W.
	Title	The workload of riding-school horses during jumping			Type
	Year	2006	Publication	Equine Veterinary Journal. Supplement	Abbreviated Journal	Equine Vet J Suppl
	Volume		Issue	36	Pages	93-97
	Keywords	Acid-Base Equilibrium/physiology; Animals; Blood Gas Analysis/veterinary; Blood Glucose/metabolism; Cross-Over Studies; Electrolytes/blood; Female; Heart Rate/physiology; Hematocrit/veterinary; Horses/blood/physiology; Lactates/blood; Male; Physical Conditioning, Animal/physiology; *Sports; Time Factors; Water-Electrolyte Balance/physiology
	Abstract	REASONS FOR PERFORMING THE STUDY: As there are no reports on the real workload of horses that jump fences, this study was undertaken in riding-school horses. OBJECTIVE: To compare the workload of horses jumping a course of fences with that of horses cantering over the same course at the same average speed without jumping fences. The workload variables included heart rate (HR), packed cell volume (PCV), acid-base balance (venous pH, pCO2, HCO3-) and blood lactate (LA), glucose, total protein and electrolyte concentrations. METHODS: Eight healthy riding-school horses performed test A (a course of approximately 700 m with 12 jumps from 0.8-1.0 m high at an average speed of approximately 350 m/min) and test B (same course at the same speed, but without the rails) in a crossover study with at least 4 h between the 2 tests. Before each test the horses were fitted with a heart rate meter (Polar Electro). Blood samples were taken from the jugular vein at rest prior to the test, after warm-up before starting the course, immediately after the course and after recovery. All samples were analysed immediately. RESULTS: The mean +/- s.d maximal HR (beats/min) during the course (184 +/- 17 and 156 +/- 21, respectively) and the mean HR after recovery (75 +/- 6 and 63 +/- 7, respectively) were significantly higher in test A compared to test B (P = 0.001 and P = 0.007 respectively). The mean LA concentrations after the course and after recovery (mmol/l) were significantly higher in test A (3.6 +/- 2.7 and 1.0 +/- 0.9, respectively) compared to test B (0.9 +/- 0.5 and 0.3 +/- 0.1, respectively), (P = 0.016 and P = 0.048 respectively). The mean PCV (I/l) after the course and after recovery was also significantly different between tests A (0.48 +/- 0.04 and 0.39 +/- 0.03, respectively) and B (0.42 +/- 0.04 and 0.36 +/- 0.03, respectively) (P<0.01). The mean pH and the mean HCO3- (mmol/l) after the course were significantly lower in test A (7.40 +/- 0.04 and 28.9 +/- 1.4, respectively) compared to test B (7.45 +/- 0.03 and 30.4 +/- 2.3, respectively) (P<0.05). CONCLUSIONS: This study indicates that in riding-school horses jumping fences, even at a low level competition, provokes a significant workload compared to cantering the same distance and speed without fences. POTENTIAL RELEVANCE: This study makes it clear that the extra workload of jumping fences should be taken into account in the training programmes of jumping horses. Further research with more experienced horses jumping higher fences will reveal the workload for top-level jumping horses.
	Address	Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 114, 3584 CM Utrecht, The Netherlands
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN		ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:17402399			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	3760
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	Author	Birch, H.L.; Bailey, A.J.; Goodship, A.E.
	Title	Macroscopic 'degeneration' of equine superficial digital flexor tendon is accompanied by a change in extracellular matrix composition			Type	Journal Article
	Year	1998	Publication	Equine Veterinary Journal	Abbreviated Journal	Equine Vet J
	Volume	30	Issue	6	Pages	534-539
	Keywords	Animals; Collagen/analysis; DNA/analysis; Extracellular Matrix/chemistry; Glycosaminoglycans/analysis; Horses/injuries/physiology; Immunohistochemistry; Rupture/veterinary; Tendon Injuries/metabolism/pathology/veterinary; Tendons/chemistry/*pathology; Water/analysis
	Abstract	Injuries to the superficial digital flexor tendon are common in horses required to gallop and jump at speed. Partial rupture of this tendon usually occurs in the central core of the midmetacarpal region and may be preceded by localised degenerative changes. Post mortem examination of apparently normal equine flexor tendons has revealed an abnormal macroscopic appearance in the central core, characterised by a reddish discolouration. We have previously shown that there is also physical damage to the collagen fibres. In the present study we tested the hypothesis that the abnormal appearance is accompanied by changes in the composition of the extracellular matrix of the tendon. Biochemical analysis of the extracellular matrix demonstrated an increase in total sulphated glycosaminoglycan content, increase in the proportion of type III collagen and decrease in collagen linked fluorescence in the central core of 'degenerated' tendons relative to tissue from the peripheral region of the same tendon. Dry matter content and total collagen content were not significantly different between tendon zones or normal and 'degenerated' tendons. These changes suggest a change in cell metabolism and matrix turnover in the central core of the tendon and are likely to contribute to a decrease in mechanical properties in this part of the tendon, predisposing to the characteristic partial rupture of the tendon.
	Address	Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hatfield, UK
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0425-1644	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:9844973			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	3794
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	Author	Steinhoff, H.J.; Lieutenant, K.; Redhardt, A.
	Title	Conformational transition of aquomethemoglobin: intramolecular histidine E7 binding reaction to the heme iron in the temperature range between 220 K and 295 K as seen by EPR and temperature-jump measurements			Type	Journal Article
	Year	1989	Publication	Biochimica et Biophysica Acta	Abbreviated Journal	Biochim Biophys Acta
	Volume	996	Issue	1-2	Pages	49-56
	Keywords	Animals; Electron Spin Resonance Spectroscopy; Heme; Histidine; Horses; Humans; Hydrogen-Ion Concentration; Methemoglobin/*ultrastructure; Motion; Protein Conformation; Temperature; Thermodynamics; Water
	Abstract	Temperature-dependent EPR and temperature-jump measurements have been carried out, in order to examine the high-spin to low-spin transition of aquomethemogobin (pH 6.0). Relaxation rates and equilibrium constants could be determined as a function of temperature. As a reaction mechanism for the high-spin to low-spin transition, the binding of N epsilon of His E7 to the heme iron had been proposed; the same mechanism had been suggested for the ms-effect, found in temperature-jump experiments on aquomethemoglobin. A comparison of the thermodynamic quantities, deduced form the measurements in this paper, gives evidence that indeed the same reaction is investigated in both cases. Our results and most of the findings of earlier studies on the spin-state transitions of aquomethemoglobin, using susceptibility, optical, or EPR measurements, can be explained by the transition of methemoglobin with H2O as ligand (with high-spin state at all temperatures) and methemoglobin with ligand N epsilon of His E7 (with a low-spin ground state). Thermal fluctuations of large amplitude have to be postulated for the reaction to take place, so this reaction may be understood as a probe for the study of protein dynamics.
	Address	Institut fur Biophysik, Ruhr-Universitat Bochum, F.R.G
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0006-3002	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:2544230			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	3803
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	Author	Andersson, P.; Kvassman, J.; Lindstrom, A.; Olden, B.; Pettersson, G.
	Title	Effect of NADH on the pKa of zinc-bound water in liver alcohol dehydrogenase			Type	Journal Article
	Year	1981	Publication	European Journal of Biochemistry / FEBS	Abbreviated Journal	Eur J Biochem
	Volume	113	Issue	3	Pages	425-433
	Keywords	Alcohol Oxidoreductases/metabolism; Aldehydes/metabolism; Animals; Binding Sites; Cinnamates/metabolism; Horses; Hydrogen-Ion Concentration; Kinetics; Ligands; Liver/metabolism; NAD/*metabolism; Water/metabolism; Zinc/metabolism
	Abstract	Equilibrium constants for coenzyme binding to liver alcohol dehydrogenase have been determined over the pH range 10--12 by pH-jump stop-flow techniques. The binding of NADH or NAD+ requires the protonated form of an ionizing group (distinct from zinc-bound water) with a pKa of 10.4. Complex formation with NADH exhibits an additional dependence on the protonation state of an ionizing group with a pKa of 11.2. The binding of trans-N,N-dimethylaminocinnamaldehyde to the enzyme . NADH complex is prevented by ionization of the latter group. It is concluded from these results that the pKa-11.2-dependence of NADH binding most likely derives from ionization of the water molecule bound at the catalytic zinc ion of the enzyme subunit. The pKa value of 11.2 thus assigned to zinc-bound water in the enzyme . NADH complex appears to be typical for an aquo ligand in the inner-sphere ligand field provided by the zinc-binding amino acid residues in liver alcohol dehydrogenase. This means that the pKa of metal-bound water in zinc-containing enzymes can be assumed to correlate primarily with the number of negatively charged protein ligands coordinated by the active-site zinc ion.
	Address
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0014-2956	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:7011796			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	3810
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