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Author |
Cilnis, M.J.; Kang, W.; Weaver, S.C. |
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Title |
Genetic conservation of Highlands J viruses |
Type |
Journal Article |
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Year |
1996 |
Publication |
Virology |
Abbreviated Journal  |
Virology |
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Volume |
218 |
Issue |
2 |
Pages |
343-351 |
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Keywords |
Alphavirus/*genetics; Alphavirus Infections/transmission/veterinary/virology; Amino Acid Sequence; Animals; Base Sequence; Conserved Sequence; Disease Outbreaks; Encephalitis, Viral/veterinary/virology; *Evolution, Molecular; Horses; Molecular Sequence Data; Phylogeny; RNA, Viral/genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Turkeys; Variation (Genetics)/*genetics |
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Abstract |
We studied molecular evolution of the mosquito-borne alphavirus Highlands J (HJ) virus by sequencing PCR products generated from 19 strains isolated between 1952 and 1994. Sequences of 1200 nucleotides including portions of the E1 gene and the 3' untranslated region revealed a relatively slow evolutionary rate estimated at 0.9-1.6 x 10(-4) substitutions per nucleotide per year. Phylogenetic trees indicated that all HJ viruses descended from a common ancestor and suggested the presence of one dominant lineage in North America. However, two or more minor lineages probably circulated simultaneously for periods of years to a few decades. Strains isolated from a horse suffering encephalitis, and implicated in a recent turkey outbreak, were not phylogenetically distinct from strains isolated in other locations during the same time periods. Our findings are remarkably similar to those we obtained previously for another North American alphavirus, eastern equine encephalomyelitis virus, with which Highlands J shares primary mosquito and avian hosts, geographical distribution, and ecology. These results support the hypotheses that the duration of the transmission season affects arboviral evolutionary rates and vertebrate host mobility influences genetic diversity. |
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Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA |
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0042-6822 |
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PMID:8610461 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2657 |
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Author |
Bannasch, D.; Rinaldo, C.; Millon, L.; Latson, K.; Spangler, T.; Hubberty, S.; Galuppo, L.; Lowenstine, L. |
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Title |
SRY negative 64,XX intersex phenotype in an American saddlebred horse |
Type |
Journal Article |
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Year |
2007 |
Publication |
Veterinary Journal (London, England : 1997) |
Abbreviated Journal |
Vet J |
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Volume |
173 |
Issue |
2 |
Pages |
437-439 |
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Keywords |
Animals; Female; Genitalia/abnormalities; Hermaphroditism/*veterinary; Horse Diseases/*diagnosis/genetics; Horses/*genetics/*physiology; Karyotyping; Phenotype; Sex Differentiation; Sex Differentiation Disorders/diagnosis/veterinary; Sex-Determining Region Y Protein/genetics/*metabolism |
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Abstract |
A female American saddlebred horse was presented for surgical correction of a possible pseudohermaphrodite condition. The horse had abnormal external genitalia and exhibited stallion-like behaviour. No evidence of uterine or ovarian tissue was identified on laparoscopic examination, but hypoplastic testicular-like tissue was removed, although this was found to contain no spermatogonia upon histopathological examination. A karyotype was performed and showed the normal chromosomal complement for a female horse (64,XX). Polymerase chain reaction to detect the SRY gene was negative in peripheral blood as well as the testicular-like tissue. This case represents the first report of an SRY negative XX-male sex reversal intersex phenotype, which is a potentially inherited condition, in an American saddlebred horse. |
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Address |
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. dlbannasch@ucdavis.edu |
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1090-0233 |
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Notes |
PMID:16386440 |
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Serial |
1882 |
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Author |
de Waal, F.B. |
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Title |
The end of nature versus nurture |
Type |
Journal Article |
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Year |
1999 |
Publication |
Scientific American |
Abbreviated Journal |
Sci Am |
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Volume |
281 |
Issue |
6 |
Pages |
94-99 |
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Keywords |
Animals; *Behavior; Behavior, Animal; Ecology; *Environment; Ethology; Evolution; Female; *Genetics; Humans; Instinct; Learning; Male; Sex Characteristics; Twin Studies |
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Abstract |
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Address |
Living Links Center, Yerkes Regional Primate Research Center, Atlanta, USA |
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English |
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ISSN |
0036-8733 |
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Notes |
PMID:10614071 |
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no |
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Call Number |
refbase @ user @ |
Serial |
192 |
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Author |
Jansen, T.; Forster, P.; Levine, M.A.; Oelke, H.; Hurles, M.; Renfrew, C.; Weber, J.; Olek, K. |
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Title |
Mitochondrial DNA and the origins of the domestic horse |
Type |
Journal Article |
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Year |
2002 |
Publication |
Proceedings of the National Academy of Sciences of the United States of America |
Abbreviated Journal |
Proc. Natl. Acad. Sci. U.S.A. |
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Volume |
99 |
Issue |
16 |
Pages |
10905-10910 |
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Keywords |
Animals; Animals, Domestic/classification/*genetics; Base Sequence; DNA, Complementary; *DNA, Mitochondrial; *Evolution, Molecular; Horses/classification/*genetics; Molecular Sequence Data; Phylogeny |
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Abstract |
The place and date of the domestication of the horse has long been a matter for debate among archaeologists. To determine whether horses were domesticated from one or several ancestral horse populations, we sequenced the mitochondrial D-loop for 318 horses from 25 oriental and European breeds, including American mustangs. Adding these sequences to previously published data, the total comes to 652, the largest currently available database. From these sequences, a phylogenetic network was constructed that showed that most of the 93 different mitochondrial (mt)DNA types grouped into 17 distinct phylogenetic clusters. Several of the clusters correspond to breeds and/or geographic areas, notably cluster A2, which is specific to Przewalski's horses, cluster C1, which is distinctive for northern European ponies, and cluster D1, which is well represented in Iberian and northwest African breeds. A consideration of the horse mtDNA mutation rate together with the archaeological timeframe for domestication requires at least 77 successfully breeding mares recruited from the wild. The extensive genetic diversity of these 77 ancestral mares leads us to conclude that several distinct horse populations were involved in the domestication of the horse. |
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Address |
Biopsytec Analytik GmbH, Marie-Curie-Strasse 1, 53359 Rheinbach, Germany. jansen@biopsytec.com |
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English |
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ISSN |
0027-8424 |
ISBN |
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Notes |
PMID:12130666 |
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no |
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Call Number |
refbase @ user @ |
Serial |
772 |
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Author |
Hostikka, S.L.; Eddy, R.L.; Byers, M.G.; Hoyhtya, M.; Shows, T.B.; Tryggvason, K. |
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Title |
Identification of a distinct type IV collagen alpha chain with restricted kidney distribution and assignment of its gene to the locus of X chromosome-linked Alport syndrome |
Type |
Journal Article |
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Year |
1990 |
Publication |
Proceedings of the National Academy of Sciences of the United States of America |
Abbreviated Journal |
Proc. Natl. Acad. Sci. U.S.A. |
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Volume |
87 |
Issue |
4 |
Pages |
1606-1610 |
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Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cloning, Molecular; Collagen/*genetics; Epitopes/analysis; Female; Fluorescent Antibody Technique; Gene Library; *Genes; Humans; Immunoblotting; Kidney/cytology/*metabolism; Macromolecular Substances; Molecular Sequence Data; Nephritis, Hereditary/*genetics; Oligopeptides/chemical synthesis/immunology; Placenta/metabolism; Pregnancy; Restriction Mapping; Sequence Homology, Nucleic Acid; *X Chromosome |
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Abstract |
We have identified and extensively characterized a type IV collagen alpha chain, referred to as alpha 5(IV). Four overlapping cDNA clones isolated contain an open reading frame for 543 amino acid residues of the carboxyl-terminal end of a collagenous domain, a 229-residue carboxyl-terminal noncollagenous domain, and 1201 base pairs coding for a 3' untranslated region. The collagenous Gly-Xaa-Yaa repeat sequence has five imperfections that coincide with those in the corresponding region of the alpha 1(IV) chain. The noncollagenous domain has 12 conserved cysteine residues and 83% and 63% sequence identity with the noncollagenous domains of the alpha 1(IV) and alpha 2(IV) chains, respectively. The alpha 5(IV) chain has less sequence identity with the putative bovine alpha 3(IV) and alpha 4(IV) chains. Antiserum against an alpha 5(IV) synthetic peptide stained a polypeptide chain of about 185 kDa by immunoblot analysis and immunolocalization of the chain in human kidney was almost completely restricted to the glomerulus. The gene was assigned to the Xq22 locus by somatic cell hybrids and in situ hybridization. This may be identical or close to the locus of the X chromosome-linked Alport syndrome that is believed to be a type IV collagen disease. |
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Address |
Biocenter, University of Oulu, Finland |
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English |
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ISSN |
0027-8424 |
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Notes |
PMID:1689491 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
5291 |
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Author |
Drent, P.J.; van Oers, K.; van Noordwijk, A.J. |
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Title |
Realized heritability of personalities in the great tit (Parus major) |
Type |
Journal Article |
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Year |
2003 |
Publication |
Proceedings. Biological sciences / The Royal Society |
Abbreviated Journal |
Proc Biol Sci |
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Volume |
270 |
Issue |
1510 |
Pages |
45-51 |
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Keywords |
Aggression; Animals; Animals, Domestic; Animals, Wild; *Behavior, Animal; Breeding; Exploratory Behavior; Female; *Heredity; Male; Selection (Genetics); Songbirds/*genetics/*physiology; Variation (Genetics) |
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Abstract |
Behaviour under conditions of mild stress shows consistent patterns in all vertebrates: exploratory behaviour, boldness, aggressiveness covary in the same way. The existence of highly consistent individual variation in these behavioural strategies, also referred to as personalities or coping styles, allows us to measure the behaviour under standardized conditions on birds bred in captivity, link the standardized measurements to the behaviour under natural conditions and measure natural selection in the field. We have bred the great tit (Parus major), a classical model species for the study of behaviour under natural conditions, in captivity. Here, we report a realized heritability of 54 +/- 5% for early exploratory behaviour, based on four generations of bi-directional artificial selection. In addition to this, we measured hand-reared juveniles and their wild-caught parents in the laboratory. The heritability found in the mid-offspring-mid-parent regression was significantly different from zero. We have thus established the presence of considerable amounts of genetic variation for personality types in a wild bird. |
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Address |
Netherlands Institute of Ecology, PO Box 40, 6666 ZG Heteren, The Netherlands. drent@cto.nioo.knaw.nl |
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English |
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ISSN |
0962-8452 |
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Notes |
PMID:12590770 |
Approved |
no |
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Call Number |
refbase @ user @ |
Serial |
591 |
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Author |
Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B. |
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Title |
Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces |
Type |
Journal Article |
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Year |
2004 |
Publication |
Parasitology |
Abbreviated Journal |
Parasitology |
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Volume |
129 |
Issue |
Pt 6 |
Pages |
733-739 |
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Keywords |
Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics |
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Abstract |
Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae. |
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Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it |
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English |
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ISSN |
0031-1820 |
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Notes |
PMID:15648696 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2631 |
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Author |
Branchi, I.; Bichler, Z.; Berger-Sweeney, J.; Ricceri, L. |
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Title |
Animal models of mental retardation: from gene to cognitive function |
Type |
Journal Article |
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Year |
2003 |
Publication |
Neuroscience and Biobehavioral Reviews |
Abbreviated Journal |
Neurosci Biobehav Rev |
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Volume |
27 |
Issue |
1-2 |
Pages |
141-153 |
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Keywords |
Animals; Animals, Genetically Modified/growth & development; Behavior/physiology; Behavior, Animal; Brain/*growth & development; Cognition/*physiology; *Disease Models, Animal; Environment; Genes; Genetic Diseases, Inborn/physiopathology; Humans; Mental Retardation/classification/*genetics/*physiopathology |
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Abstract |
About 2-3% of all children are affected by mental retardation, and genetic conditions rank among the leading causes of mental retardation. Alterations in the information encoded by genes that regulate critical steps of brain development can disrupt the normal course of development, and have profound consequences on mental processes. Genetically modified mouse models have helped to elucidate the contribution of specific gene alterations and gene-environment interactions to the phenotype of several forms of mental retardation. Mouse models of several neurodevelopmental pathologies, such as Down and Rett syndromes and X-linked forms of mental retardation, have been developed. Because behavior is the ultimate output of brain, behavioral phenotyping of these models provides functional information that may not be detectable using molecular, cellular or histological evaluations. In particular, the study of ontogeny of behavior is recommended in mouse models of disorders having a developmental onset. Identifying the role of specific genes in neuropathologies provides a framework in which to understand key stages of human brain development, and provides a target for potential therapeutic intervention. |
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Address |
Section of Behavioural Pathophysiology, Laboratorio di Fisiopatologia di Organo e di Sistema, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy. branchi@iss.it |
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English |
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ISSN |
0149-7634 |
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Notes |
PMID:12732230 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2805 |
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Author |
Novacek, M.J. |
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Title |
Mammalian phylogeny: shaking the tree |
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Journal Article |
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Year |
1992 |
Publication |
Nature |
Abbreviated Journal |
Nature |
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Volume |
356 |
Issue |
6365 |
Pages |
121-125 |
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Keywords |
Animals; Evolution; Fossils; Mammals/classification/*genetics; *Phylogeny |
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Abstract |
Recent palaeontological discoveries and the correspondence between molecular and morphological results provide fresh insight on the deep structure of mammalian phylogeny. This new wave of research, however, has yet to resolve some important issues. |
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Address |
American Museum of Natural History, New York 10024 |
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English |
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ISSN |
0028-0836 |
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Notes |
PMID:1545862 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3546 |
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Author |
Sebastiani, F.; Meiswinkel, R.; Gomulski, L.M.; Guglielmino, C.R.; Mellor, P.S.; Malacrida, A.R.; Gasperi, G. |
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Title |
Molecular differentiation of the Old World Culicoides imicola species complex (Diptera, Ceratopogonidae), inferred using random amplified polymorphic DNA markers |
Type |
Journal Article |
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Year |
2001 |
Publication |
Molecular Ecology |
Abbreviated Journal |
Mol Ecol |
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Volume |
10 |
Issue |
7 |
Pages |
1773-1786 |
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Keywords |
Africa; Animals; Ceratopogonidae/*classification/*genetics; Ecology; Evolution, Molecular; Female; *Genetic Markers; Madagascar; Phylogeny; *Polymorphism, Genetic; *Random Amplified Polymorphic DNA Technique; Variation (Genetics) |
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Abstract |
Samples of seven of the 10 morphological species of midges of the Culicoides imicola complex were considered. The importance of this species complex is connected to its vectorial capacity for African horse sickness virus (AHSV) and bluetongue virus (BTV). Consequently, the risk of transmission may vary dramatically, depending upon the particular cryptic species present in a given area. The species complex is confined to the Old World and our samples were collected in Southern Africa, Madagascar and the Ivory Coast. Genomic DNA of 350 randomly sampled individual midges from 19 populations was amplified using four 20-mer primers by the random amplified polymorphic DNA (RAPD) technique. One hundred and ninety-six interpretable polymorphic bands were obtained. Species-specific RAPD profiles were defined and for five species diagnostic RAPD fragments were identified. A high degree of polymorphism was detected in the species complex, most of which was observed within populations (from 64 to 76%). Principal coordinate analysis (PCO) and cluster analysis provided an estimate of the degree of variation between and within populations and species. There was substantial concordance between the taxonomies derived from morphological and molecular data. The amount and the different distributions of genetic (RAPD) variation among the taxa can be associated to their life histories, i.e. the abundance and distribution of the larval breeding sites and their seasonality. |
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Address |
Department of Animal Biology, Laboratory of Zoology, University of Pavia, Piazza Botta 9, I-27100 Pavia, Italy |
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English |
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Edition |
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ISSN |
0962-1083 |
ISBN |
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Notes |
PMID:11472544 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2647 |
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