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	Author	Steinhoff, H.J.; Schrader, J.; Schlitter, J.
	Title	Temperature-jump studies and polarized absorption spectroscopy of methemoglobin-thiocyanate single crystals			Type	Journal Article
	Year	1992	Publication	Biochimica et Biophysica Acta	Abbreviated Journal	Biochim Biophys Acta
	Volume	1121	Issue	3	Pages	269-278
	Keywords	Animals; Crystallization; Horses; Kinetics; Methemoglobin/chemistry; Solutions; Spectrum Analysis; Temperature; Thiocyanates/chemistry
	Abstract	Association equilibria and association kinetics of the thiocyanate binding reaction to methemoglobin in single crystals and solution are studied using temperature-jump technique and polarized absorption spectroscopy. Different kinetic constants are found for the reaction in solution and crystal phase for the alpha- and beta-subunits of the methemoglobin tetramer. The reduction of the reactivity of the alpha- and beta-subunits in crystalline phase is 6-fold and 2.4-fold, respectively, compared to the values found in solution. The intramolecular binding reaction of the N epsilon of the distal histidine E7 which is observed in methemoglobin in solution cannot be detected in single crystals. Our results suggest that crystallization of hemoglobin has little influence on small-scale structural fluctuations which are necessary for ligands to get to the binding sites and large-scale structural motions are suppressed.
	Address	Institut fur Biophysik, Ruhr-Universitat Bochum, Germany
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0006-3002	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:1627604			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	3800
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