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Author  |
Kuckelkorn, B. |
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Title |
Assessment of pregnancy in Kiang mares (Equus hemionus holdereri ) using estrogen determination in feces |
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Journal Article |
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Year |
1994 |
Publication |
Theriogenology |
Abbreviated Journal |
Theriogenology |
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Volume |
42 |
Issue |
1 |
Pages |
37-42 |
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Abstract |
Analysis of fecal estrogens was used to diagnose pregnancy in 6 Kiang mares (Equus hemionus holdereri ) that were kept at Tierpark Berlin. Three extraction methods were compared and were followed by an established RIA for total estrogen. Extraction of desiccated feces with chloroform/n-hexane and KOH, with and without enzyme hydrolysis showed better results than extraction with diethylether without hydrolysis. Pregnancy was confirmed by observation of foaling in 2 mares that showed estrogen concentrations between 800 and 1800 ng/g and in 1 mare that showed widely fluctuating values between 500 and 1300 ng/g of feces. Two mares with estrogen concentrations below 500 ng/g were not seen to foal. The method using chloroform/n-hexane and KOH without enzyme hydrolysis seems practical for non-invasive evaluation of the endocrine status in this endangered Equidae species. |
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Address |
Institute for Zoo Biology and Wildlife Research Alfred-Kowalke-Str. 17, 10315 Berlin, Germany |
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Language |
English |
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Original Title |
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Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0093-691X |
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Notes |
PMID:16727510 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2335 |
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