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Author (up) Kuckelkorn, B. doi  openurl
  Title Assessment of pregnancy in Kiang mares (Equus hemionus holdereri ) using estrogen determination in feces Type Journal Article
  Year 1994 Publication Theriogenology Abbreviated Journal Theriogenology  
  Volume 42 Issue 1 Pages 37-42  
  Keywords  
  Abstract Analysis of fecal estrogens was used to diagnose pregnancy in 6 Kiang mares (Equus hemionus holdereri ) that were kept at Tierpark Berlin. Three extraction methods were compared and were followed by an established RIA for total estrogen. Extraction of desiccated feces with chloroform/n-hexane and KOH, with and without enzyme hydrolysis showed better results than extraction with diethylether without hydrolysis. Pregnancy was confirmed by observation of foaling in 2 mares that showed estrogen concentrations between 800 and 1800 ng/g and in 1 mare that showed widely fluctuating values between 500 and 1300 ng/g of feces. Two mares with estrogen concentrations below 500 ng/g were not seen to foal. The method using chloroform/n-hexane and KOH without enzyme hydrolysis seems practical for non-invasive evaluation of the endocrine status in this endangered Equidae species.  
  Address Institute for Zoo Biology and Wildlife Research Alfred-Kowalke-Str. 17, 10315 Berlin, Germany  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0093-691X ISBN Medium  
  Area Expedition Conference  
  Notes PMID:16727510 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2335  
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