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	Author	Breen, M.; Downs, P.; Irvin, Z.; Bell, K.
	Title	Intrageneric amplification of horse microsatellite markers with emphasis on the Przewalski's horse (E. przewalskii)			Type	Journal Article
	Year	1994	Publication	Animal Genetics	Abbreviated Journal	Anim Genet
	Volume	25	Issue	6	Pages	401-405
	Keywords	Animals; DNA, Satellite/genetics; Gene Amplification; Gene Frequency; Genetic Markers; Heterozygote; Horses/genetics; Species Specificity
	Abstract	Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was calculated to be 0.9994. The results suggest that it should be possible to use E. caballus-derived microsatellite markers to provide parentage verification and additional valuable information to the captive management of E. przewalskii. The ability to amplify corresponding loci in the remaining five species of the genus was also confirmed, illustrating the general application of markers isolated from the domestic horse to the evaluation of polymorphism in the other six species of the genus.
	Address	Australian Equine Blood Typing Research Laboratory, University of Queensland, St Lucia
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0268-9146	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:7695120			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	2246
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