Abstract: Horses have been considered to rely on human gesticular cues (McKinley and Sambrook 2000, Anim Cogn 3:13-22; and recently Maros et al. 2008, Anim Cogn 11:457-466), however large variability among individuals tested in two-ways object choice tasks was found. Part of the horses in those studies (40 and 26 %, respectively) even failed to pass adequately through the training session which preceded the testing phase and served to learn a horse to carry out a task. Therefore, we alternated the experimental design designed by McKinley and Sambrook (reduced number of testing trials to 10 per horse to keep its attention, applied just one, a dynamic-sustained pointing cue with touching the bucket, etc.), and tested an effect of training method, sex, age, and learning on proportion of correct choices. We hypothesised, that horses trained by “traditional” method (TTM) will get lower score than those experienced with “horsemanship-based” methods (HTM), being characterized by closer and more frequent human-horse contact and also extended exercising “from the ground” with frequent using of arms cues. Despite simplification of the methods, only about 60 % of tested horses passed through the training phase (i.e., learned to come to and upturn the bucket with hidden treat). Successful completion of training phase was reached regardless of age or sex of a horse, but by the training method; HTM horses ran better compared to TTM ones. No significant effect of age, sex, or learning (i.e., trial order within all 10), and training method as well was found on proportion of correct trials in the testing phase. Horses made a correct choice in more than 70% of trials. Individual scores ranged from 50 to 100 %. In conclusion, horses showed high level of comprehension of human pointing gesture, regardless of their sex or age. No effects of training method or learning process within a test suggest low impact of handling and learning on the level of comprehension at least of the most vivid human pointing gesture. Horses trained by methods based on “natural human-horse communication” did enhance cooperation with people.

Abstract: According to scientists human-horse relationship is influenced by management conditions (Hausberger et al.; 2007) including handling methods (Henry et al.; 2005). Furthermore, Henry et al. (2005) show that mares foals" behaviour toward humans may be influenced even by the mares. Although the familiarity of the testperson is said to be an important variable measuring relationship (Waiblinger et al.; 2006), other studies show that horses generalize from the experimenter to unfamiliar humans (Henry et al.; 2005) or from the caretaker to other human beings (Hausberger et al.; 2002).
Methods: 51 horses were observed in a 20x20 sandy arena (familiar to the subjects) to evaluate their relationship with a testperson.
First an arena test was carried out for 5 minutes. The horse was put into the arena alone. After that the person test came in which the testperson interacted with the horse.
The person test consisted of 3 or 4 different phases according to the horse"s behaviour. The phases were: (1.) voluntary animal approach test in which the testperson stood still and the subject was allowed to move wherever it wanted (Appr-vol); (2.) the testperson calling the horse to himself (Appr-call); (3.) the testperson making the horse stay beside him (Stand-still); (4.) the testperson making the horse follow him (without any tools and food reward) (Follow).
First 51 horses were tested with testpersons who were familiar to the animals. After that 39 horses were retested with unfamiliar testpersons within more than one week.
To analyze the potential effects of different human-related factors on the horse behaviour in the person test, such as time spent with training the horse (grooming, riding, working etc.), training methods, number of trainers etc. a questionnare has been used.
Results: Arena test: Horse behaviour was affected neither by their age (2-24 years) nor by their gender (30 geldings and 1 stallion vs 20 mares), however, mares spent more time in the entrance of the arena.
In the test with unfamiliar testpersons (retest) horses stood longer and walked less, which could reflect some habituation to the situation.
Person test: Gender and age of horses or time (years) elapsing since the horse started to be trained did not have any effect on the reactions of horses.
There was a positive correlation between the time (hours) spent with the horse weekly and the time of Follow.
The number of trainers had a significant effect on the Appr-vol and Follow responses: the less the number of the trainers, the sooner the horse approached the familiar testperson and the longer the animal followed him.
In the person test, significant difference was observed only in Appr-vol, thus horses followed the familiar testpersons much longer than the unfamiliar ones. Horses trained to follow their trainers without a lead rope followed significantly longer than animals without this kind of trainings.
Conclusion: Followership seemed to be the most sensitive behavioural response to human familiarity and it may also indicate some other aspects of horse-human relationship, however, training has a clear effect on this human related reaction.

Abstract: BACKGROUND: Foraging in groups offers animals a number of advantages, such as increasing their likelihood of finding food or detecting and avoiding predators. In order for a group to remain together, there has to be some degree of coordination of behaviour and movement between its members (which may in some cases be initiated by a decision-making leader, and in other cases may emerge as an underlying property of the group). For example, behavioural synchronisation is a phenomenon where animals within a group initiate and then continue to conduct identical behaviours, and has been characterised for a wide range of species. We examine how a pair of animals should behave using a state-dependent approach, and ask what conditions are likely to lead to behavioural synchronisation occurring, and whether one of the individuals is more likely to act as a leader. RESULTS: The model we describe considers how the energetic gain, metabolic requirements and predation risks faced by the individuals affect measures of their energetic state and behaviour (such as the degree of behavioural synchronisation seen within the pair, and the value to an individual of knowing the energetic state of its colleague). We explore how predictable changes in these measures are in response to changes in physiological requirements and predation risk. We also consider how these measures should change when the members of the pair are not identical in their metabolic requirements or their susceptibility to predation. We find that many of the changes seen in these measures are complex, especially when asymmetries exist between the members of the pair. CONCLUSION: Analyses are presented that demonstrate that, although these general patterns are robust, care needs to be taken when considering the effects of individual differences, as the relationship between individual differences and the resulting qualitative changes in behaviour may be complex. We discuss how these results are related to experimental observations, and how the model and its predictions could be extended.

Abstract: We investigated 8 horses in five therapeutic riding centres situated in San Cataldo (Caltanissetta – I), Nicosia (Catania – II), Pellaro (Reggio Calabria – III), San Gregorio (Catania – IV), Niguarda Hospitals (Milan – V). The managements of the animals were of different typologies: Type 1, Type 2 and Type 3. In type 1 the horses were used for therapeutic riding only. Furthermore intra and interspecific social interactions were not allowed. In type 2 the horses played kinetic activities and made social interactions. In type 3 the horses were free in paddock, as intra and interspecific social interactions were allowed. The centre I, with a management of type 1, housed 1 horse (A1); the centre II, with a management of type 2, housed 1 horse (B2); the centre III, with a management of type 1, housed 1 horse (C1); the centre IV, with a management of type 2, housed 2 horses (D2 and E2); the centre V, with a management of type 3, housed 3 horses (F3, G3 and H3). Breeds of horses were: Anglo-Arab (n°1), Avelignese (n°3), Italian Selle (n°3), draught-horse crossbreed (n°1). They were 2 geldings and 6 females. Their ages ranged from 12 to 23 years. We observed a total of 64 patients affected by different pathologies: autism, motory handicap, blindness and deafness, children“s cerebral paralysis, relational problems, mental deficiency, Down”s syndrome.
The horses" behaviour was observed at rest and during therapeutic activities with these patients. The Heart Rate (HR) was used as physiological parameter for an ethological evaluation, measured by a telemetric heart rate monitor (Polar Horse Trainer). Horses were analysed with a reactivity test for emotional homeostasis evaluation, too. Heart rate values were studied with non parametrical statistical analysis methods.
Distinct management typologies provided statistically different basal mean values of heart rate (intergroup and intragroup): Type 1 vs Type 2 (P~0.05) and Type 1 vs Type 3 (P<0.05). The comparison of heart rate during therapeutic activities of diverse management showed the following results: A1 vs B2 (P<0.05), B2 vs C1 (P<0.05), Type 1 vs Type 2 (P<0.01). Different managements, both in the same or different typologies, gave significantly diverse results (A1 vs E2: P~0.05; C1 vs E2: P~0.05; B2 vs E2: P~0.05).
This study shows that the statistic differences obtained by therapies with autistic patients derive from management conditions of Type 1. In reactivity test there aren"t any significant differences among the three management typologies. However, we recorded strong variation between medium and maximum values of heart rate, especially in Type 1 and Type 2 of management.
These high variations of heart rate indicated fear reaction of the horse to new stimuli.
This reaction could lead to dangerous accidents for patients during therapeutic activities.
Horses used in therapeutic riding programs must be evaluated before this employment. Horse's behaviour can be assessed by an ethological observation and a reactivity test. Furthermore, the horses must be guaranteed welfare conditions and must live in an environment enriched with sensorial stimuli and respectful of their physiological and ethological needs.

Abstract: Cribbiting is a common stereotypy in domestic horses and is associated with stress and low fibre/high concentrate diets, which are known to cause gastric inflammation and ulceration. Recent studies indicate that antacid supplements can reduce both gastric inflammation and cribbiting. However, stereotypies are also associated with altered brain functions and these are thought to be responsible for emancipation. Emancipation refers to a process by which stereotypies become increasingly independent of the causal factors and may therefore persist with age, regardless of the conditions. Emancipation complicates therapy and points to the importance of prevention.
To further examine the relationship between gastric health and cribbiting and its relation to age at onset, we examined the effect of the proton-pump inhibitor omeprazole (GastroGard, Merial) on gastric inflammation/ulceration and on cribbiting in a double-blind placebo-controlled study. Thirty-one horses of different sex, breed and age were randomly allocated to treatment and placebo and divided into three groups depending on age: 0-5 years (n=11), 5-10 years (n=11) and >10 years (n=9), whereby age at onset of cribbiting was &#8804; 5 years for all horses. Treatment and placebo were administered orally by syringe once every day for 28 days by the horse owners. Treatment consisted of 4mg/kg omeprazole for the first four days and 1mg/kg for the following 24days.
Using scan sampling and behaviour sampling, general activity and the number of crib-bites were assessed in the home pens one day before and one day between day 21 and 28 of treatment. Observations were made for three hours each around morning and evening feeding times, respectively. Prior to pre-treatment observations and after post-treatment observations, the horses were transported to the clinic and endoscoped for signs of inflammation and ulceration in the oesophagus, the squamous area, the glandular area and the first part of the duodenum using a common scoring system (Andrews et al. 1999).
All 31 horses showed signs of gastric inflammation/ulceration which decreased significantly from a median score of 1,2 to 0,6 in the treatment group (Wilcoxon signed rank test, z=-3,186, p=0,001), while there was no change in the placebo group (z=-0,577, p=1.0). However, there was no significant difference in cribbiting before and after treatment, neither in the treatment group (z=-1,834, p=0,068), nor in the placebo group (z=-0,415, p=0,734). Moreover, there were no significant effects of age on gastric inflammation/ulceration (2-way ANOVA, F=1,112, p=0,345) or cribbiting (F=1,113, p=0,344).
These results contrast with the recent studies on the effects of antacids on gastric health and cribbiting. There are two possible explanations for this: First, cribbiting may become rapidly established and may thus have persisted even in the youngest age group. Second, omeprazole acts specifically by reducing gastric acid secretion into stomach, while antacids may also have an effect on the intestine. However, these results shed doubts on the hypothesis that cribbiting is caused by gastric inflammation/ulceration.