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Author |
SCHOEN AMS et al, |
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Title |
Behavior of young shetland and welsh ponies |
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Journal Article |
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Year |
1976 |
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Abbreviated Journal |
Biol Behav |
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Volume |
1 |
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192-216 |
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Notes |
from Professor Hans Klingels Equine Reference List |
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no |
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1577 |
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Author |
Schaller, G.B: |
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Title |
The Serengeti Lion: A Study of Predator-Prey Relations (Wildlife Behavior and Ecology series) |
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Book Whole |
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Year |
1976 |
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University Of Chicago Press |
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Chicago |
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978-0226736402 |
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no |
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Call Number |
Equine Behaviour @ team @ |
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5159 |
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Author |
Rodier, F. |
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Title |
[Spectral properties of porcine plasminogen: study of the acidic transition (author's transl)] |
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Journal Article |
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Year |
1976 |
Publication |
European journal of biochemistry / FEBS |
Abbreviated Journal |
Eur J Biochem |
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Volume |
63 |
Issue |
2 |
Pages |
553-562 |
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Keywords |
Animals; Binding Sites; Guanidines; Hydrogen-Ion Concentration; *Plasminogen; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Swine; Temperature |
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Abstract |
The acidic transition of porcine plasminogen, prepared by affinity chromatography, was studied by non-destructive methods. These methods are based on the analysis of the behaviour of the tryptophyls under various conditions. The perturbation of the absorption and emission spectra by pH or temperature and the dynamic quenching of the intrinsic fluorescence are used to obtain information on structural changes which affect the environment of these residues. It is shown that by decreasing pH the fluorescence emission spectra are shifted toward the long wavelengths, with a broadening of the fluorescence band. The same effect can be obtained at constant pH by heating the protein solution. In order to analyze these phenomena, it is assumed that the fluorescence intensities at 355 nm and 328 nm reflect the proportion of the tryptophans which are exposed to the solvent, and buried, respectively. The plot of the ratio of the fluorescence intensities at these wavelengths versus pH or temperature leads to a titration curve showing an unmasking of tryptophans. The proportion of exposed tryptophans is measured by the dynamic fluorescence quenching technique and the data analyzed according to Lehrer. The plot of the fraction of exposed tryptophyls versus pH also shows the unmasking of these chromophores. Thermal perturbation of a solution of plaminogen at neutral pH induces a difference absorption spectrum whose amplitudes at the maxima are proportional to the number of exposed aromatic residues. The comparison with a solution of fully denatured plasminogen in 6 M guanidium chloride, where all the tryptophyls are exposed, shows that the percentage of exposure is equal to 59%. This number is significantly higher than the percentage found by the fluorescence quenching technique (20%), indicating that some tryptophyls are located in crevices, exposed to the solvent but not to the iodide. At acidic pH the absorption difference spectra induced by thermal perturbation are not classical, since they show an inversion and a new band between 300 nm and 305 nm. This band is mentioned in the literature as a minor band of tryptophan which appears when this chromophore is located in an asymmetric environment. On plotting the maximum amplitude of these spectra obtained at acidic pH versus temperature, we obtain a curve indicating that two types of antagonistic interactions are involved in the perturbation of the chromophores spectra. The spectrophotometric titration of plasminogen gives classical absorption difference spectra. By plotting the maximum amplitude at 292 nm versus pH, we obtain a titration curve with an apparent pK of 2.9 units. This pK is acidic which respect to the pK value of a normal carboxyl. This low value can be due to a positively charged group in the neighbourhood of a carboxyl, which interacts with one or more chromophores. When the carboxyl becomes protonated, this positively charged group is free and available to perturb the environment of some chromophores... |
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French |
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Original Title |
Proprietes spectrales du plasminogene porcin. Etude de la transition acide |
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ISSN |
0014-2956 |
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Notes |
PMID:4326 |
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no |
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Call Number |
Admin @ knut @ |
Serial |
22 |
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Author |
Rashek Va, |
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Title |
Details of feeding and feeding behaviour in young wild asses on Barsa – Kelmes Island (Aralsea) |
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Journal Article |
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Year |
1976 |
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Abbreviated Journal |
Zool Zh |
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Volume |
55 |
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Pages |
784-787 |
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Notes |
from Professor Hans Klingels Equine Reference List |
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no |
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Call Number |
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Serial |
1495 |
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Author |
Ngethe Jc, |
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Title |
Preference and daily intake of five east african grasses by zebras |
Type |
Journal Article |
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Year |
1976 |
Publication |
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Abbreviated Journal |
J Range Mgmt |
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Volume |
29 |
Issue |
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Pages |
510 |
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Notes |
from Professor Hans Klingels Equine Reference List |
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no |
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Serial |
1416 |
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Author |
Mcknight T, |
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Title |
Friendly Vermin – A survey of feral livestock in Australia. Chapter III: Feral donkeys |
Type |
Journal Article |
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Year |
1976 |
Publication |
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Abbreviated Journal |
Univ Cal Press |
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Volume |
21 |
Issue |
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Pages |
22-29 |
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Notes |
from Professor Hans Klingels Equine Reference List |
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no |
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Serial |
1380 |
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Author |
McGregor, P.K.; Dabelsteen, T. |
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Title |
Communication Networks |
Type |
Book Chapter |
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Year |
1976 |
Publication |
Ecology and evolution of acoustic communication in birds |
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Volume |
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Issue |
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Pages |
409-425 |
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Cornell University Press |
Place of Publication |
Ithaca |
Editor |
Kroodsma, D. E.; Miller, E. H. |
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Language |
Englisch |
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ISBN |
978-0801482212 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2167 |
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Author |
Macfadden Bj, |
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Title |
Cladistic analysis of primitive equids, with notes on other perissodactyls |
Type |
Journal Article |
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Year |
1976 |
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Abbreviated Journal |
Syst Zool |
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Volume |
25 |
Issue |
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Pages |
1.14 |
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Notes |
from Professor Hans Klingels Equine Reference List |
Approved |
no |
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Serial |
1359 |
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Author |
Lutta, A.S. |
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Title |
[Distribution and biology of Heptatoma pellucens in the Karelian ASSR (fam. Tabanidae)] |
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Journal Article |
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Year |
1976 |
Publication |
Parazitologiia |
Abbreviated Journal |
Parazitologiia |
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Volume |
10 |
Issue |
1 |
Pages |
53-55 |
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Keywords |
Animals; Biology; *Diptera; Ecology; Female; Horses/parasitology; Larva; Russia |
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Abstract |
The analysis is given of the peculiarities of the distribution of the widely spread forest subspecies Heptatoma pellucens pellucens Fabr. in the northern part of its distribution area in Karelia. Some data on the biology of the larva of this subspecies are presented. |
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Russian |
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Original Title |
K rasprostraneniiu i biologii Heptatoma pellucens v Karel'skoi ASSR (sem. Tabanidae) |
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ISSN |
0031-1847 |
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Notes |
PMID:133320 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2701 |
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Author |
Kirkpatrick, J.F.; Vail, R.; Devous, S.; Schwend, S.; Baker, C.B.; Wiesner, L. |
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Title |
Diurnal variation of plasma testosterone in wild stallions |
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Journal Article |
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Year |
1976 |
Publication |
Biology of reproduction |
Abbreviated Journal |
Biol Reprod |
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Volume |
15 |
Issue |
1 |
Pages |
98-101 |
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Keywords |
Animals; *Circadian Rhythm; Horses/*blood; Male; Montana; Sexual Behavior, Animal; Species Specificity; Testosterone/*blood |
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Language |
English |
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ISSN |
0006-3363 |
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Notes |
PMID:986195 |
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no |
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Call Number |
refbase @ user @ |
Serial |
149 |
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