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Author |
Nicol, C.J.; Yoon, M.; Ward, J.M.; Yamashita, M.; Fukamachi, K.; Peters, J.M.; Gonzalez, F.J. |
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Title |
PPARgamma influences susceptibility to DMBA-induced mammary, ovarian and skin carcinogenesis |
Type |
Journal Article |
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Year |
2004 |
Publication |
Carcinogenesis |
Abbreviated Journal |
Carcinogenesis |
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Volume |
25 |
Issue |
9 |
Pages |
1747-1755 |
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Keywords |
9,10-Dimethyl-1,2-benzanthracene/*toxicity; Animals; DNA Primers/chemistry; Disease Susceptibility; Female; Heterozygote; Humans; Mammary Neoplasms, Experimental/chemically induced/*pathology; Mice; Ovarian Neoplasms/chemically induced/*pathology; RNA, Messenger/genetics/metabolism; Receptors, Cytoplasmic and Nuclear/genetics/*physiology; Reverse Transcriptase Polymerase Chain Reaction; Skin Neoplasms/chemically induced/*pathology; Survival Rate; Transcription Factors/genetics/*physiology; Zinc Fingers |
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Abstract |
Peroxisome proliferator-activated receptor gamma (PPARgamma), a member of the nuclear receptor superfamily, plays a role in adipocyte differentiation, type II diabetes, macrophage response to inflammation and is suggested to influence carcinogen-induced colon cancer. Studies done in vitro and in vivo also revealed that PPARgamma ligands might promote differentiation and/or regression of mammary tumors. To directly evaluate the role of PPARgamma in mammary carcinogenesis, PPARgamma wild-type (+/+) or heterozygous (+/-) mice were administered 1 mg 7,12-dimethylbenz[a]anthracene (DMBA) by gavage once a week for 6 weeks and followed for a total of 25 weeks. Compared with congenic PPARgamma(+/+) littermate controls, PPARgamma(+/-) mice had early evidence for increased susceptibility to DMBA-mediated carcinogenesis based on a 1.6-fold increase in the percentage of mice with skin papillomas, as well as a 1.7-fold increase in the numbers of skin papillomas per mouse (P < 0.05). Similarly, PPARgamma(+/-) mice also had a 1.5-fold decreased survival rate (P = 0.059), and a 1.7-fold increased incidence of total tumors per mouse (P < 0.01). Moreover, PPARgamma(+/-) mice had an almost 3-fold increase in mammary adenocarcinomas (P < 0.05), an over 3-fold increase in ovarian granulosa cell carcinomas (P < 0.05), an over 3-fold increase in malignant tumors (P < 0.02) and a 4.6-fold increase in metastatic incidence. These results are the first to demonstrate an increased susceptibility in vivo of PPARgamma haploinsufficiency to DMBA-mediated carcinogenesis and suggest that PPARgamma may act as a tumor modifier of skin, ovarian and breast cancers. The data also support evidence suggesting a beneficial role for PPARgamma-specific ligands in the chemoprevention of mammary, ovarian and skin carcinogenesis. |
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Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA |
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0143-3334 |
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PMID:15073042 |
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no |
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Call Number |
refbase @ user @ |
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76 |
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Author |
Nosek, J. |
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Title |
The ecology and public health importance of Dermacentor marginatus and D. reticulatus ticks in Central Europe |
Type |
Journal Article |
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Year |
1972 |
Publication |
Folia Parasitologica |
Abbreviated Journal |
Folia Parasitol (Praha) |
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Volume |
19 |
Issue |
1 |
Pages |
93-102 |
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Keywords |
Animals; Arthropod Vectors; Birds; Cattle; Czechoslovakia; Deer; Dermacentor/physiology; Dogs; Ecology; Encephalitis, Tick-Borne; Europe; Female; Goats; Horses; Insectivora; Male; Mice; Rodentia; Sheep; Swine; *Ticks |
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0015-5683 |
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PMID:4670812 |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2720 |
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Author |
Reynhout, I.C.; Cornelissen, J.J.L.M.; Nolte, R.J.M. |
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Title |
Self-assembled architectures from biohybrid triblock copolymers |
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Journal Article |
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Year |
2007 |
Publication |
Journal of the American Chemical Society |
Abbreviated Journal |
J Am Chem Soc |
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Volume |
129 |
Issue |
8 |
Pages |
2327-2332 |
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Keywords |
Horseradish Peroxidase/*chemistry; Micelles; Molecular Structure; Myoglobin/*chemistry; Particle Size; Polyethylene Glycols/*chemistry; Polymers/*chemical synthesis/chemistry; Polystyrenes/*chemistry; Surface-Active Agents/chemical synthesis/chemistry |
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Abstract |
The synthesis and self-assembly behavior of biohybrid ABC triblock copolymers consisting of a synthetic diblock, polystyrene-b-polyethylene glycol (PSm-b-PEG113), where m is varied, and a hemeprotein, myoglobin (Mb) or horse radish peroxidase (HRP), is described. The synthetic diblock copolymer is first functionalized with the heme cofactor and subsequently reconstituted with the apoprotein or the apoenzyme to yield the protein-containing ABC triblock copolymer. The obtained amphiphilic block copolymers self-assemble in aqueous solution into a large variety of aggregate structures. Depending on the protein and the polystyrene block length, micellar rods, vesicles, toroids, figure eight structures, octopus structures, and spheres with a lamellar surface are formed. |
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Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands |
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0002-7863 |
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Notes |
PMID:17274615 |
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no |
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Call Number |
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Serial |
1832 |
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Author |
Rumiantsev, S.N. |
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Title |
[Biological function of Clostridium tetani toxin (ecological and evolutionary aspects)] |
Type |
Journal Article |
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Year |
1973 |
Publication |
Zhurnal Evoliutsionnoi Biokhimii i Fiziologii |
Abbreviated Journal |
Zh Evol Biokhim Fiziol |
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Volume |
9 |
Issue |
5 |
Pages |
474-480 |
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Keywords |
Animals; Cats; Chickens; Dogs; Ecology; Evolution; Goats; Guinea Pigs; Haplorhini; Horses; Insectivora; Mice; Perissodactyla; Rabbits; Rats; Sheep; *Tetanus Toxin |
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Russian |
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Original Title |
K voprosu biologicheskoi funktsii toksina Clostridium tetani (ekologicheskie i evolutsionnye aspekty |
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0044-4529 |
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PMID:4203684 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2713 |
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Author |
Scherer, W.F.; Dickerman, R.W. |
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Title |
Ecologic studies of Venezuelan encephalitis virus in southeastern Mexico. 8. Correlations and conclusions |
Type |
Journal Article |
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Year |
1972 |
Publication |
The American Journal of Tropical Medicine and Hygiene |
Abbreviated Journal |
Am J Trop Med Hyg |
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Volume |
21 |
Issue |
2 |
Pages |
86-89 |
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Keywords |
Animals; Birds; Cattle; Chiroptera; Cricetinae; Culex; Culicidae; *Disease Reservoirs; Ecology; Encephalitis Virus, Venezuelan Equine/immunology; Encephalitis Viruses/*isolation & purification; Encephalomyelitis, Equine/epidemiology/*etiology; Horses; Humans; *Insect Vectors; Mammals; Mexico; Mice; Opossums; Rats; Swine |
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0002-9637 |
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Notes |
PMID:4399844 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2721 |
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Author |
Tempelis, C.H.; Nelson, R.L. |
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Title |
Blood-feeding patterns of midges of the Culicoides variipennis complex in Kern County, California |
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Journal Article |
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Year |
1971 |
Publication |
Journal of Medical Entomology |
Abbreviated Journal |
J Med Entomol |
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Volume |
8 |
Issue |
5 |
Pages |
532-534 |
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Keywords |
Animals; Behavior, Animal; Cattle; Ceratopogonidae/*immunology; Chickens; Dogs; Ecology; Feeding Behavior; Female; Horses; Humans; Immune Sera; Mice; Precipitin Tests; Rabbits; Rats; Sciuridae; Sheep |
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0022-2585 |
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Notes |
PMID:5160258 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2723 |
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Author |
Touma, C.; Palme, R.; Sachser, N. |
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Title |
Analyzing corticosterone metabolites in fecal samples of mice: a noninvasive technique to monitor stress hormones |
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Journal Article |
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Year |
2004 |
Publication |
Hormones and Behavior |
Abbreviated Journal |
Horm Behav |
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Volume |
45 |
Issue |
1 |
Pages |
10-22 |
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Keywords |
Adrenal Cortex/drug effects; Adrenal Cortex Function Tests; Adrenocorticotropic Hormone/pharmacology; Analysis of Variance; Animals; Circadian Rhythm; Corticosterone/*analysis/metabolism; Dexamethasone/pharmacology; Feces/*chemistry; Female; Immunoenzyme Techniques/*methods; Male; Mice; Mice, Inbred C57BL; Models, Animal; Reproducibility of Results; Stress, Psychological/*metabolism |
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Abstract |
In small animals like mice, the monitoring of endocrine functions over time is constrained seriously by the adverse effects of blood sampling. Therefore, noninvasive techniques to monitor, for example, stress hormones in these animals are highly demanded in laboratory as well as in field research. The aim of our study was to evaluate the biological relevance of a recently developed technique to monitor stress hormone metabolites in fecal samples of laboratory mice. In total, six experiments were performed using six male and six female mice each. Two adrenocorticotropic hormone (ACTH) challenge tests, two dexamethasone (Dex) suppression tests and two control experiments [investigating effects of the injection procedure itself and the diurnal variation (DV) of glucocorticoids (GCs), respectively] were conducted. The experiments clearly demonstrated that pharmacological stimulation and suppression of adrenocortical activity was reflected accurately by means of corticosterone metabolite (CM) measurements in the feces of males and females. Furthermore, the technique proved sensitive enough to detect dosage-dependent effects of the ACTH/Dex treatment and facilitated to reveal profound effects of the injection procedure itself. Even the naturally occurring DV of GCs could be monitored reliably. Thus, our results confirm that measurement of fecal CM with the recently established 5alpha-pregnane-3beta,11beta,21-triol-20-one enzyme immunoassay is a very powerful tool to monitor adrenocortical activity in laboratory mice. Since mice represent the vast majority of all rodents used for research worldwide and the number of transgenic and knockout mice utilized as animal models is still increasing, this noninvasive technique can open new perspectives in biomedical and behavioral science. |
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Address |
Department of Behavioural Biology, University of Muenster, D-48149 Muenster, Germany. touma@uni-muenster.de |
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English |
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0018-506X |
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Notes |
PMID:14733887 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
4084 |
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Author |
Touma, C.; Sachser, N.; Mostl, E.; Palme, R. |
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Title |
Effects of sex and time of day on metabolism and excretion of corticosterone in urine and feces of mice |
Type |
Journal Article |
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Year |
2003 |
Publication |
General and Comparative Endocrinology |
Abbreviated Journal |
Gen Comp Endocrinol |
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Volume |
130 |
Issue |
3 |
Pages |
267-278 |
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Keywords |
Animals; Chromatography, High Pressure Liquid; Circadian Rhythm/*physiology; Corticosterone/*metabolism/urine; Feces/*chemistry; Female; Immunoenzyme Techniques; Kinetics; Male; Mice; Mice, Inbred C57BL; Reference Values; Sex Factors; Stress/metabolism; Time Factors; Tritium |
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Abstract |
Non-invasive techniques to monitor stress hormones in small animals like mice offer several advantages and are highly demanded in laboratory as well as in field research. Since knowledge about the species-specific metabolism and excretion of glucocorticoids is essential to develop such a technique, we conducted radiometabolism studies in mice (Mus musculus f. domesticus, strain C57BL/6J). Each mouse was injected intraperitoneally with 740 kBq of 3H-labelled corticosterone and all voided urine and fecal samples were collected for five days. In a first experiment 16 animals (eight of each sex) received the injection at 9 a.m., while eight mice (four of each sex) were injected at 9 p.m. in a second experiment. In both experiments radioactive metabolites were recovered predominantly in the feces, although males excreted significantly higher proportions via the feces (about 73%) than females (about 53%). Peak radioactivity in the urine was detected within about 2h after injection, while in the feces peak concentrations were observed later (depending on the time of injection: about 10h postinjection in experiment 1 and about 4h postinjection in experiment 2, thus proving an effect of the time of day). The number and relative abundance of fecal [3H]corticosterone metabolites was determined by high performance liquid chromatography (HPLC). The HPLC separations revealed that corticosterone was extensively metabolized mainly to more polar substances. Regarding the types of metabolites formed, significant differences were found between males and females, but not between the experiments. Additionally, the immunoreactivity of these metabolites was assessed by screening the HPLC fractions with four enzyme immunoassays (EIA). However, only a newly established EIA for 5alpha-pregnane-3beta,11beta,21-triol-20-one (measuring corticosterone metabolites with a 5alpha-3beta,11beta-diol structure) detected several peaks of radioactive metabolites with high intensity in both sexes, while the other EIAs showed only minor immunoreactivity. Thus, our study for the first time provides substantial information about metabolism and excretion of corticosterone in urine and feces of mice and is the first demonstrating a significant impact of the animals' sex and the time of day. Based on these data it should be possible to monitor adrenocortical activity non-invasively in this species by measuring fecal corticosterone metabolites with the newly developed EIA. Since mice are extensively used in research world-wide, this could open new perspectives in various fields from ecology to behavioral endocrinology. |
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Address |
Department of Behavioral Biology, Institute of Neuro and Behavioral Biology, University of Muenster, Badestrasse 9, D-48149 Muenster, Germany. touma@uni-muenster.de |
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0016-6480 |
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Notes |
PMID:12606269 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
4086 |
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Author |
Valova, G.P.; Mefod'ev, V.V. |
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Title |
[Specific features of an epidemic process in leptospiroses in northern conditions in Western Siberia] |
Type |
Journal Article |
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Year |
1972 |
Publication |
Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii |
Abbreviated Journal |
Zh Mikrobiol Epidemiol Immunobiol |
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Volume |
49 |
Issue |
11 |
Pages |
138-145 |
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Keywords |
Animals; Bird Diseases/epidemiology; Birds; Carnivora; Cattle; Cattle Diseases/epidemiology; Dog Diseases/epidemiology; Dogs; Ecology; Foxes; Horse Diseases/epidemiology; Horses; Humans; Insectivora; Leptospirosis/*epidemiology/veterinary; Mice; Rats; Reindeer; Rodent Diseases/epidemiology; Rodentia; Sheep; Sheep Diseases/epidemiology; Siberia |
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Russian |
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Original Title |
Nekotorye spetsificheskie cherty epidemicheskogo protsessa pri leptospirozakh v usloviiakh Severa v Zapadnoi Sibiri |
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0372-9311 |
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Notes |
PMID:4645851 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2718 |
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Author |
Yamada, T.; Rojanasuphot, S.; Takagi, M.; Wungkobkiat, S.; Hirota, T. |
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Title |
Studies on an epidemic of Japanese encephalitis in the northern region of Thailand in 1969 and 1970 |
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Journal Article |
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Year |
1971 |
Publication |
Biken Journal |
Abbreviated Journal |
Biken J |
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Volume |
14 |
Issue |
3 |
Pages |
267-296 |
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Keywords |
Adolescent; Adult; Animals; Arboviruses/immunology; Buffaloes; Cattle; Chickens; Child; Child, Preschool; Cross Reactions; Culicidae; Dengue Virus/immunology; Disease Outbreaks; Ducks; Ecology; Encephalitis Virus, Japanese/immunology/isolation & purification; Encephalitis, Japanese/cerebrospinal fluid/*epidemiology/immunology/microbiology/mortality; Female; Hemagglutination Inhibition Tests; Hemorrhagic Fevers, Viral/epidemiology; Horses; Humans; Infant; Male; Mice; Neutralization Tests; Swine; Thailand |
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English |
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Abbreviated Series Title |
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0006-2324 |
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Notes |
PMID:4400462 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2728 |
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