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Author Zeitler-Feicht, M. H.; Streit, S.; Dempfle, L. openurl 
  Title (up) Automatic feeding systems for horses in group housing systems with regard to animal welfare. Part 2: Comparison of different automatic feeding systems Type Journal Article
  Year 2011 Publication Tierärztliche Praxis Großtiere Abbreviated Journal Tierärztl Prax  
  Volume 39 Issue G Pages 33-40  
  Keywords Pferd, Fütterungssystem, Bauvarianten, soziale Interaktionen, Besuchshäufigkeit, Aufenthaltsdauer, Herzfrequenz, Verletzungsrisiko [Horse, feeding system, different types of construction, social interactions, visiting frequency, duration of stay, heart rate, risk of injury]  
  Abstract Ziel: Überprüfung der Tiergerechtheit von in der Bauweise unterschiedlichen

Futterabrufstationen für Pferde in Gruppenhaltung anhand ethologischer

und physiologischer Parameter. Material und Methoden: In 32

Offenlaufställen (452 Pferde) mit computergesteuerten Abrufstationen

für Kraftfutter und Heu erfolgten kontinuierliche Direktbeobachtungen

für je einen 24-Stunden-Tag (Tortenstückverfahren). Erfasst wurden: Aufenthaltsdauer,

Besuchshäufigkeit, Droh- und Meideverhalten im Fütterungsbereich,

Blockaden sowie Herzfrequenz und Integumentverletzungen.

Ergebnisse: Eine Durchlaufstation reduzierte die Zahl der Auseinandersetzungen

im Fütterungsbereich signifikant. Ansonsten erhöhten die

für das fressende Pferd tiergerechten Varianten (Fressstand mit Eingangssperre,

ohne Austreibehilfe) Besuchshäufigkeit und Aufenthaltsdauer und

steigerten somit auch die Anzahl an Drohgesten je Tier und Tag. Insgesamt

betrachtet kann jedoch die Anzahl an sozionegativen Interaktionen

im Fütterungsbereich der Abrufstationen als relativ gering eingestuft

werden. Die Herzfrequenz lag im Warteareal bei den meisten Pferden im

physiologischen Bereich (45,1 ± 12,42 Schläge/min), erhöhte sich jedoch

in der Abrufstation um ca. 20 Schläge/min. Einige Tiere zeigten möglicherweise

stressbedingt kurzfristig auffallend hohe Werte (≥ 100 Schläge/

min). Integumentverletzungen im Zusammenhang mit dem Fütterungssystem

traten nicht auf. Der wichtigste Einflussfaktor auf die Untersuchungskriterien

war der Betrieb (Fläche, Konzeption, Management).

Schlussfolgerung: Durchlaufstationen sind pferdegerechter als Rücklaufstationen.

Nicht tiergerecht sind Stationen mit stromführender Austreibehilfe.

Weitere bauliche Unterschiede der derzeitigen Futterabrufstationen

dürften eher von untergeordneter Bedeutung sein, vor allem da

Flächengebot und Konzeption der Offenstallanlage sowie das Management

die überprüften Kriterien zur Tiergerechtheit maßgeblich beeinflussen.

Untersuchungen zur Abklärung der Ursache für die vereinzelt aufgetretenen

sehr hohen Herzfrequenzwerte sollten durchgeführt werden.

Objective: Comparison with regard to animal welfare of different automatic

feeding systems for hay and concentrate in group housing systems

for horses using parameters of ethology and physiology. Material and

methods: Parameters of research comprised: duration of stay, frequency

of visit, threatening behaviour with and without risk of injury, and avoiding

behaviour as well as heart rate and injuries of the integument. 452

horses were observed at the feeding area of 32 run-out-sheds. Every group

of horses was continuously observed following the pie chart system for

24 hours. Results: The “walk-through” station significantly reduced the

number of conflicts in the feeding area, whereas those systems which are

appropriate for the feeding horses (feeding station with access barrier and

without stimulation device by electric shock) led to a higher frequency of

visits and a longer duration of stay resulting in more threatening gestures.

However, the number of negative interactions in the feeding area of the

feeding systems can all together be classified as relatively insignificant.

The heart rate was within the physiological range (45.1 ± 12.42 beats/min)

in the waiting area, but increased by approximately 20 beats/min on average

within the feeding station. Some horses showed a very high heart rate

(≥ 100 beats/min) while entering the feeding station, possibly stress-related.

There were no injuries of the integument associated with the feeding

systems. The most important factor of the observation criteria was the

individual group housing system with its different dimensions, conception

and management. Conclusion: “Walk-through” stations are better than

”walk-back” stations with regard to animal welfare. Likewise, automatic

feeding stations with a current-carrying stimulation device are not supportive

of good welfare. The other differences between the constructions

of feeding stations of present systems are probably of less importance, particularly

as it was shown that the stable (management, stable area, conception)

had a significant influence on the surveyed parameters. Investigations

to obtain information on the cause for the sporadic occurrence of

very high heart rate values should be undertaken.]
 
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 5766  
Permanent link to this record
 

 
Author Turner, K.K.; Nielsen, B.D.; O'Connor, C.I.; Burton, J.L. doi  openurl
  Title (up) Bee pollen product supplementation to horses in training seems to improve feed intake: A pilot study Type Journal Article
  Year 2006 Publication Journal of Animal Physiology and Animal Nutrition Abbreviated Journal J Anim Physiol Anim Nutr (Berl)  
  Volume 90 Issue 9-10 Pages 414-420  
  Keywords *Animal Nutrition Physiology; Animals; Antibody Formation; Bees; Detergents; Dietary Fiber/metabolism; Dietary Supplements; *Digestion; Eating/*drug effects; Exercise Test/veterinary; Female; Heart Rate/drug effects/physiology; Horses/blood/immunology/*physiology; Leukocyte Count/*veterinary; Male; Oxygen Consumption/drug effects/physiology; Physical Conditioning, Animal/*physiology; Pilot Projects; *Pollen; Random Allocation  
  Abstract The objective of this study was to determine the efficacy of supplementation of Dynamic Trio 50/50, a bee pollen-based product, to improve physical fitness, blood leukocyte profiles, and nutritional variables in exercised horses. Ten Arabian horses underwent a standardised exercise test (SET), then were pair-matched by sex and fitness and randomly assigned to BP (receiving 118 g of Dynamic Trio 50/50 daily) or CO (receiving 73 g of a placebo) for a period of 42 days. A total collection was conducted from days 18 to 21 on six geldings to determine nutrient retention and neutral detergent fibre (NDF) and acid detergent fibre (ADF) digestibility. Horses were exercise conditioned and completed another SET on day 42. V160 and V200 were calculated from SET heart rates (HR). Lactate, glucose, haematocrit (HT) and haemoglobin (HB) concentrations were determined from SET blood samples. Total leukocyte count, and circulating numbers of various leukocytes and IgG, IgM and IgA concentrations were determined in rest and recovery blood samples from both SETs. Geldings on BP (n = 3) ate more feed than CO. BP had less phosphorus excretion, and tended to retain more nitrogen. BP tended to digest more NDF and ADF while having lower NDF digestibility and tending to have lower ADF digestibility. No treatment differences existed for V160 and V200, HR, lactate, HT and HB. There was a trend for lymphocyte counts to be lower in BP than CO on day 42. Dynamic Trio 50/50 supplementation may have a positive effect on performance by helping horses in training meet their potentially increased nutrient demands by increasing feed intake and thus nutrient retention.  
  Address Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA. kturner@uga.edu  
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  Series Volume Series Issue Edition  
  ISSN 0931-2439 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:16958799 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4237  
Permanent link to this record
 

 
Author Warren-Smith, A.K.; Greetham, L.; McGreevy, P.D. url  doi
openurl 
  Title (up) Behavioral and physiological responses of horses (Equus caballus) to head lowering Type Journal Article
  Year 2007 Publication Journal of Veterinary Behavior: Clinical Applications and Research Abbreviated Journal  
  Volume 2 Issue 3 Pages 59-67  
  Keywords behavior; head lowering; heart rate; horse; training  
  Abstract Horse trainers often report that lowering the height of a horse's head so the poll is below the height of the withers can induce a calming effect during training. Four groups of horses were used in a 2-part study to investigate the behavioral and physiological effects of head lowering in horses. In Part 1, Group A had no experimental stimuli applied and horses in Group B were trained to lower their heads when presented with a specific stimulus by the handler. The stimulus for head lowering was the application of downward pressure on the headcollar via the lead rope until the horse lowered its head such that its lips were approximately at mid-cannon (third metacarpal) height, whereupon the pressure was released. The stimulus was applied again if the horse raised its head during the 300-second test period. In Part 2, Groups C and D were aroused until their heart rates exceeded 100 beats per minute (bpm). Group C had no further experimental stimuli applied whereas Group D lowered their heads as a response to the above stimulus for a period of 300 seconds. Repeated measures analysis showed that there was no difference between the heart rate of Groups A and B or Groups C and D but that the heart rate of Groups A and B were lower than Groups C and D during the 300-second post-arousal (P < 0.001). The horses in Groups A and B were more likely to contact the handler (P < 0.001), exhibit licking and chewing (P < 0.001), rest a hindleg (P < 0.001), and sniff the ground (P < 0.001) than those in Groups C and D. The number of stimuli required to maintain the head in a lowered position was greatest during the first 30 seconds (P = 0.012 and P < 0.001, Parts 1 and 2, respectively). The current study has shown that head lowering in horses does not influence cardiac responses, even after the horses had been aroused to have their heart rates above 100 bpm. Therefore, it is not a method that will aid in calming an aroused horse in training. Contrary to popular belief, there was no association with licking-and-chewing and head lowering, nor with these behaviors and response acquisition.  
  Address  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 4201  
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Author Leliveld, L.M.C.; Düpjan, S.; Tuchscherer, A.; Puppe, B. url  doi
openurl 
  Title (up) Behavioural and physiological measures indicate subtle variations in the emotional valence of young pigs Type Journal Article
  Year 2016 Publication Physiology & Behavior Abbreviated Journal Physiol. Behav.  
  Volume 157 Issue Pages 116-124  
  Keywords Emotion; Heart rate; Vocalisation; Emotional valence; Animal welfare; Domestic pig  
  Abstract Abstract In the study of animal emotions, emotional valence has been found to be difficult to measure. Many studies of farm animals' emotions have therefore focussed on the identification of indicators of strong, mainly negative, emotions. However, subtle variations in emotional valence, such as those caused by rather moderate differences in husbandry conditions, may also affect animals' mood and welfare when such variations occur consistently. In this study, we investigated whether repeated moderate aversive or rewarding events could lead to measurable differences in emotional valence in young, weaned pigs. We conditioned 105 female pigs in a test arena to either a repeated startling procedure (sudden noises or appearances of objects) or a repeated rewarding procedure (applesauce, toy and straw) over 11 sessions. Control pigs were also regularly exposed to the same test arena but without conditioning. Before and after conditioning, we measured heart rate and its variability as well as the behavioural reactions of the subjects in the test arena, with a special focus on detailed acoustic analyses of their vocalisations. The behavioural and heart rate measures were analysed as changes compared to the baseline values before conditioning. A limited number of the putative indicators of emotional valence were affected by the conditioning. We found that the negatively conditioned pigs showed changes that were significantly different from those in control pigs, namely a decrease in locomotion and an increase in standing. The positively conditioned pigs, however, showed a stronger increase in heart rate and a smaller decrease in SDNN (a heart rate variability parameter indicating changes in autonomic regulation) compared to the controls. Compared to the negatively conditioned pigs, the positively conditioned pigs produced fewer vocalisations overall as well as fewer low-frequency grunts but more high-frequency grunts. The low-frequency grunts of the negatively conditioned pigs also showed lower frequency parameters (bandwidth, maximum frequency, 25% and 50% quartiles) compared to those of the positively conditioned pigs. In any of the statistically significant results, the conditioning accounted for 1.5–11.9% of variability in the outcome variable. Hence, we conclude that repeated moderate aversive and rewarding events have weak but measurable effects on some aspects of behaviour and physiology in young pigs, possibly indicating changes in emotional valence, which could ultimately affect their welfare. The combination of ethophysiological indicators, i.e., the concurrent examination of heart rate measures, behavioural responses and especially vocalisation patterns, as used in the current study, might be a useful way of examining subtle effects on emotional valence in further studies.  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0031-9384 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6017  
Permanent link to this record
 

 
Author Merkies, K.; McKechnie, M.J.; Zakrajsek, E. doi  openurl
  Title (up) Behavioural and physiological responses of therapy horses to mentally traumatized humans Type Journal Article
  Year 2018 Publication Applied Animal Behaviour Science Abbreviated Journal  
  Volume Issue Pages  
  Keywords Equine-assisted therapy; Ptsd; Horse; Behaviour; Cortisol; Heart rate  
  Abstract The benefits to humans of equine-assisted therapy (EAT) have been well-researched, however few studies have analyzed the effects on the horse. Understanding how differing mental states of humans affect the behaviour and response of the horse can assist in providing optimal outcomes for both horse and human. Four humans clinically diagnosed and under care of a psychotherapist for Post-Traumatic Stress Disorder (PTSD) were matched physically to four neurotypical control humans and individually subjected to each of 17 therapy horses loose in a round pen. A professional acting coach instructed the control humans in replicating the physical movements of their paired PTSD individual. Both horses and humans were equipped with a heart rate (HR) monitor recording HR every 5secs. Saliva samples were collected from each horse 30 min before and 30 min after each trial to analyze cortisol concentrations. Each trial consisted of 5 min of baseline observation of the horse alone in the round pen after which the human entered the round pen for 2 min, followed by an additional 5 min of the horse alone. Behavioural observations indicative of stress in the horse (gait, head height, ear orientation, body orientation, distance from the human, latency of approach to the human, vocalizations, and chewing) were retrospectively collected from video recordings of each trial and analyzed using a repeated measures GLIMMIX with Tukey's multiple comparisons for differences between treatments and time periods. Horses moved slower (p < 0.0001), carried their head lower (p < 0.0001), vocalized less (p < 0.0001), and chewed less (p < 0.0001) when any human was present with them in the round pen. Horse HR increased in the presence of the PTSD humans, even after the PTSD human left the pen (p < 0.0001). Since two of the PTSD/control human pairs were experienced with horses and two were not, a post-hoc analysis showed that horses approached quicker (p < 0.016) and stood closer (p < 0.0082) to humans who were experienced with horses. Horse HR was lower when with inexperienced humans (p < 0.0001) whereas inexperienced human HR was higher (p < 0.0001). Horse salivary cortisol did not differ between exposure to PTSD and control humans (p > 0.32). Overall, behavioural and physiological responses of horses to humans are more pronounced based on human experience with horses than whether the human is diagnosed with a mental disorder. This may be a reflection of a directness of movement associated with humans who are experienced with horses that makes the horse more attentive. It appears that horses respond more to physical cues from the human rather than emotional cues. This knowledge is important in tailoring therapy programs and justifying horse responses when interacting with a patient in a therapy setting.  
  Address  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0168-1591 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6385  
Permanent link to this record
 

 
Author Leleu, C.; Cotrel, C. openurl 
  Title (up) Body composition in young standardbreds in training: relationships to body condition score, physiological and locomotor variables during exercise Type Journal Article
  Year 2006 Publication Equine Veterinary Journal. Supplement Abbreviated Journal Equine Vet J Suppl  
  Volume Issue 36 Pages 98-101  
  Keywords Adipose Tissue/metabolism; Animals; Body Composition/*physiology; Body Constitution/*physiology; Body Weight/physiology; Exercise Test/veterinary; Female; Gait/physiology; Heart Rate/*physiology; Horses/*physiology; Lactates/blood; Male; Muscle, Skeletal/metabolism; Physical Conditioning, Animal/*physiology  
  Abstract REASONS FOR PERFORMING STUDY: Body composition is an essential factor in athletic performance of human sprinters and long distance runners. However, in horses, many questions remain concerning relationships between body composition and performance in the different equine activities. OBJECTIVES: To determine relationships between body composition, body score, physiological and locomotor variables in a population of young Standardbreds in training. METHODS: Twenty-four 2-year-old Standardbreds were studied, body condition on a scale 0-5 and bodyweight recorded, and height at withers measured. Percentage of fat (%F), fat mass (FM) and fat free mass (FFM) were estimated echographically. During a standardised exercise test on the track, velocity, heart rate, respiratory frequency and blood lactate concentrations were measured. V4 and V200 (velocity for a blood lactate concentration of 4 mmol/l and velocity of 200 beats/min) calculated. Basic gait variables were measured at 3 different speeds with an accelerometric device. RESULTS: Body composition variables: %F and FM were significantly related to body condition score and physiological variables. Body score was highly correlated to %F (r = 0.64) and FM (r = 0.71). V4 was negatively correlated to %F (r = -0.59) and FM (r = -0.60), P<0.05. V200 was also negatively related to %F and FM, (r = -0.39 and r = -0.37, respectively, P<0.1). No relationships were found between body composition and gait characteristics. CONCLUSIONS: Body composition was closely related to indirect measurements of aerobic capacity, which is a major factor of athletic performance in middle distance running horses. POTENTIAL RELEVANCE: As in human athletes, trainers should take special note to evaluate optimal bodyweight and body composition of race horses to optimise performance.  
  Address Pegase Mayenne, Departement de Medecine du Sport, Centre Hospitalier, 53 015 Laval, France  
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  Language English Summary Language Original Title  
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  Area Expedition Conference  
  Notes PMID:17402400 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4015  
Permanent link to this record
 

 
Author Hillidge, C.J.; Lees, P. openurl 
  Title (up) Cardiac output in the conscious and anaesthetised horse Type Journal Article
  Year 1975 Publication Equine veterinary journal Abbreviated Journal Equine Vet J  
  Volume 7 Issue 1 Pages 16-21  
  Keywords Anesthesia, Inhalation/*veterinary; Animals; Carbon Dioxide/blood; *Cardiac Output/veterinary; *Consciousness; Electrocardiography/veterinary; Ether, Ethyl; Female; Halothane; Heart Rate; Heart Ventricles/physiology; Horses/*physiology; Hydrogen-Ion Concentration; Male; Oxygen/blood; Posture  
  Abstract Cardiac output in the horse was measured before and at predetermined times during 2-hour periods of thiopentone-halothane and thiopentone-diethyl ether anaesthesia. Left ventricular stroke volume was decreased to a similar extent during anaesthesia with each volatile agent, but a greater reduction in cardiac output occurred during halothane anaesthesia. This finding reflected the differing effects of halothane and ether on heart rate, a slight bradycardia occurring with the former agent while ether produced a small degree of tachycardia. The latter effect was attributed to enhanced sympathoadrenal activity. Changes in cardiac output and stroke volume were considered in relation to other factors, including arterial blood pH and tensions of oxygen and carbon dioxide. Positive correlations between some of these variables and cardiac function were established. With both volatile agents the reductions in stroke volume and cardiac output were related to the duration of anaesthesia, being greatest during the early stages. Possible reasons for the tendency of stroke volume and cardiac output to return towards control levels are discussed.  
  Address  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0425-1644 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:234842 Approved no  
  Call Number refbase @ user @ Serial 102  
Permanent link to this record
 

 
Author Schmidt, A.; Aurich, J.; Möstl, E.; Müller, J.; Aurich, C. url  doi
openurl 
  Title (up) Changes in cortisol release and heart rate and heart rate variability during the initial training of 3-year-old sport horses Type Journal Article
  Year 2010 Publication Hormones and Behavior Abbreviated Journal Horm Behav  
  Volume 58 Issue 4 Pages 628-636  
  Keywords Horse; Initial training; Cortisol; Heart rate variability  
  Abstract Based on cortisol release, a variety of situations to which domestic horses are exposed have been classified as stressors but studies on the stress during equestrian training are limited. In the present study, Warmblood stallions (n = 9) and mares (n = 7) were followed through a 9 respective 12-week initial training program in order to determine potentially stressful training steps. Salivary cortisol concentrations, beat-to-beat (RR) interval and heart rate variability (HRV) were determined. The HRV variables standard deviation of the RR interval (SDRR), RMSSD (root mean square of successive RR differences) and the geometric means standard deviation 1 (SD1) and 2 (SD2) were calculated. Nearly each training unit was associated with an increase in salivary cortisol concentrations (p < 0.01). Cortisol release varied between training units and occasionally was more pronounced in mares than in stallions (p < 0.05). The RR interval decreased slightly in response to lunging before mounting of the rider. A pronounced decrease occurred when the rider was mounting, but before the horse showed physical activity (p < 0.001). The HRV variables SDRR, RMSSD and SD1 decreased in response to training and lowest values were reached during mounting of a rider (p < 0.001). Thereafter RR interval and HRV variables increased again. In contrast, SD2 increased with the beginning of lunging (p < 0.05) and no changes in response to mounting were detectable. In conclusion, initial training is a stressor for horses. The most pronounced reaction occurred in response to mounting by a rider, a situation resembling a potentially lethal threat under natural conditions.  
  Address  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0018-506x ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 5223  
Permanent link to this record
 

 
Author Schmidt, A.; Biau, S.; Möstl, E.; Becker-Birck, M.; Morillon, B.; Aurich, J.; Faure, J.-M.; Aurich, C. url  doi
openurl 
  Title (up) Changes in cortisol release and heart rate variability in sport horses during long-distance road transport Type Journal Article
  Year 2010 Publication Domestic Animal Endocrinology Abbreviated Journal Domest Anim Endocrinol  
  Volume 38 Issue 3 Pages 179-189  
  Keywords Horse; Transport; Cortisol; Heart rate variability  
  Abstract It is widely accepted that transport is stressful for horses, but only a few studies are available involving horses that are transported regularly and are accustomed to transport. We determined salivary cortisol immunoreactivity (IR), fecal cortisol metabolites, beat-to-beat (RR) interval, and heart rate variability (HRV) in transport-experienced horses (N = 7) in response to a 2-d outbound road transport over 1370 km and 2-d return transport 8 d later. Salivary cortisol IR was low until 60 min before transport but had increased (P < 0.05) 30 min before loading. Transport caused a further marked increase (P < 0.001), but the response tended to decrease with each day of transport. Concentrations of fecal cortisol metabolites increased on the second day of both outbound and return transports and reached a maximum the following day (P < 0.001). During the first 90 min on Day 1 of outbound transport, mean RR interval decreased (P < 0.001). Standard deviations of RR interval (SDRR) decreased transiently (P < 0.01). The root mean square of successive RR differences (RMSSD) decreased at the beginning of the outbound and return transports (P < 0.01), reflecting reduced parasympathetic tone. On the first day of both outbound and return transports, a transient rise in geometric HRV variable standard deviation 2 (SD2) occurred (P < 0.01), indicating increased sympathetic activity. In conclusion, transport of experienced horses leads to increased cortisol release and changes in heart rate and HRV, which is indicative of stress. The degree of these changes tended to be most pronounced on the first day of both outbound and return transport.  
  Address  
  Corporate Author Thesis  
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  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0739-7240 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 5386  
Permanent link to this record
 

 
Author Jezierski T., Gorecka A. openurl 
  Title (up) Changes in the horses heart rate during different levels of social isolation Type Journal Article
  Year 2000 Publication Animal Science Papers and Reports Abbreviated Journal Anim. Sci. Pap. Rep.  
  Volume 18 Issue 1 Pages 33-41  
  Keywords horse; heart rate; human-animal relation  
  Abstract Social isolation in horses may be regarded as a stress factor which implies welfare problems. The aim of the experiment was to examine the effect of different levels of transient social isolation and human presence on the heart rate (HR) in horses. Seven horses were used and the experiment was conducted in a tether-stable without boxes. The HR was recorded electronically, continuously for 40 min during the following test situations: all horses in the stable; experimenter approaches the tested horse, other horses being untied and leaving the stable; tested horse staying alone or in the company of one or two stable-mates; the experimenter attempts to calm the isolated horse; outdoor auditory stimuli from other horses. The HR was significantly higher during the whole period of isolation, and depended on how many horses were left as company for the one tested. The highest HR was observed while other horses were leaving the stable and during perception of outdoor auditory stimuli from others. When in the company of two stable mates, the HR was elevated only while other horses were leaving the stable and during auditory stimuli from outdoors. Human presence evoked a significant increase in HR, probably due to conditioning of horses (expecting to be untied and allowed to join the others), irrespectively whether the tested horse was left alone or with one or two stable-mates.  
  Address  
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  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 4816  
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