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Author Dalmau, A.; Ferret, A.; Chacon, G.; Manteca, X.
Title Seasonal Changes in Fecal Cortisol Metabolites in Pyrenean Chamois Type Journal Article
Year 2007 Publication Journal of Wildlife Management Abbreviated Journal J Wildl Manag
Volume 71 Issue 1 Pages 190-194
Keywords Cadí-Moixeró, Nature Reserve, chamois, cortisol metabolites, feces, hunting reserve, Pyrenees, Rupicapra pyrenaica pyrenaica, seasonal rhythm, stress
Abstract (down) We studied seasonal changes in fecal cortisol metabolites (FCM), which have been widely used as indicators of stress, in a population of Pyrenean chamois (Rupicapra pyrenaica pyrenaica) in the Cadí Range of northeastern Spain. We collected fecal samples from 2001 to 2003 in 3 particular locations with different altitudes and male or female presence, and we analyzed them for FCM and fecal nitrogen as an indicator of diet quality. We observed a clear seasonal pattern, with the highest FCM in winter, and we obtained correlations between FCM and monthly mean minimum temperatures and fecal nitrogen. We observed no effects of tourism presence, trophy hunting, or rut season on FCM. Analysis of cortisol metabolites in feces can be a good measure of winter stress in Pyrenean chamois.
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Call Number Equine Behaviour @ team @ Serial 4254
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Author Li, C.; Jiang, Z.; Tang, S.; Zeng, Y.
Title Influence of enclosure size and animal density on fecal cortisol concentration and aggression in Pere David's deer stags Type Journal Article
Year 2007 Publication General and Comparative Endocrinology Abbreviated Journal Gen Comp Endocrinol
Volume 151 Issue 2 Pages 202-209
Keywords *Aggression; Animals; *Deer; *Environment; Feces/*chemistry; Handling (Psychology); Housing, Animal; Hydrocortisone/*analysis; Male; Population Density
Abstract (down) We investigated the impact of enclosure size and animal density on behavior and adrenocortical secretion in Pere David's deer in Dafeng Nature Reserve, China. From February 15 to April 16 in 2004, we conducted two experiments. First, we studied maintenance behavior and conflict behavior of Pere David's deer stags in a large enclosure (200 ha) with low animal density (0.66 deer/ha) and a small display pen (0.75 ha) with high animal density (25.33 deer/ha). The maintenance behavior we recorded included standing, locomotion, foraging and rest. During the behavioral observations, we collected fresh voided fecal samples from the stags periodically, and analyzed the fecal cortisol concentrations in those samples using radioimmunoassay technique. Second, we monitored the fecal cortisol concentrations of one group of stags (12 deer lived in an enclosure of 100 ha) before and after transferred into a small pen (0.5 ha). We found that in the first experiment: (1) there were significant differences in standing and rest whereas no significant differences of locomotion and foraging between the free-ranging group and the display group; (2) frequency of conflict behavior in the display group was significantly higher than those in the free-ranging group; and (3) fecal cortisol concentration of the display group (326.17+/-16.98 ng/g dry feces) was significantly higher than that of the free-ranging group (268.98+/-15.21 ng/g dry feces). In the second experiment, there was no significant difference of the fecal cortisol concentrations among sampling days, but the mean fecal cortisol concentration of the day after transferring (337.46+/-17.88 ng/g dry feces) was significantly higher than that of the day before transferring (248.44+/-7.99 ng/g dry feces). Comparison with published findings, our results indicated that enclosure size and animal density affect not only behaviors, but also adrenocortical secretion in Pere David's deer. Small living space with high animal density may impose physiological stress to captive Pere David's deer. Moreover, long-term physiological stress and increase of conflict behavior may subsequently affect survival and reproduction of the deer.
Address Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
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ISSN 0016-6480 ISBN Medium
Area Expedition Conference
Notes PMID:17324429 Approved no
Call Number Equine Behaviour @ team @ Serial 5475
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Author Stahlbaum, C.C.; Houpt, K.A.
Title The role of the Flehmen response in the behavioral repertoire of the stallion Type Journal Article
Year 1989 Publication Physiology & behavior Abbreviated Journal Physiol. Behav.
Volume 45 Issue 6 Pages 1207-1214
Keywords Animals; Behavior, Animal/*physiology; Circadian Rhythm; Discrimination (Psychology)/physiology; Estrus; Feces; Female; Horses/*physiology; Male; Sexual Behavior, Animal/*physiology; Smell/*physiology; Taste/physiology; Urine
Abstract (down) The role of the Flehmen response in equine behavior was investigated under field and laboratory conditions. In Experiment 1, a field study made of five stallions on pasture with between three and eighteen mares each during the season indicated the following: 1) The Flehmen response was most frequently preceded by nasal, rather than oral, investigation of substances; 2) The stallions' rate of Flehmen varied with the estrous cycles of the mares; 3) The rate of Flehmen response did not show a variation with time of day; and 4) The Flehmen response was most frequently followed by marking behaviors rather than courtship behaviors. The results suggest that the Flehmen response is not an immediate component of sexual behavior, e.g., courtship of the stallion but may be involved in the overall monitoring of the mare's estrous cycle. Therefore the Flehmen response may contribute to the chemosensory priming of the stallion for reproduction. In Experiment 2 stallions were presented with urine or feces of mares in various stages of the reproductive cycle as well as with their own or other males' urine or feces. The occurrence of sniffing and Flehmen was used to determine the discriminatory ability of the stallions. Stallions can differentiate the sex of a horse on the basis of its feces alone, but cannot differentiate on the basis of urine. This ability may explain the function of fecal marking behavior of stallions.
Address New York State College of Veterinary Medicine, Cornell University, Ithaca 14853
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ISSN 0031-9384 ISBN Medium
Area Expedition Conference
Notes PMID:2813545 Approved no
Call Number refbase @ user @ Serial 44
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Author Griffin, B.
Title The use of fecal markers to facilitate sample collection in group-housed cats Type Journal Article
Year 2002 Publication Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science Abbreviated Journal Contemp Top Lab Anim Sci
Volume 41 Issue 2 Pages 51-56
Keywords Animals; Behavior, Animal; Biological Markers/*analysis; Cats/*physiology/psychology; Diet/veterinary; Feces/*chemistry; Food Coloring Agents/analysis; Housing, Animal; Individuality; Plastics/analysis; Specimen Handling/methods/*veterinary
Abstract (down) The provision of proper social housing is a priority when designing an experiment using domestic cats as laboratory animals. When animals are group-housed, studies requiring analysis of stool samples from individual subjects pose difficulty in sample collection and identification. In this study, commercially available concentrated food colorings (known as bakers pastes) were used as fecal markers in group-housed cats. Cats readily consumed 0.5 ml of bakers paste food coloring once daily in canned cat food. Colorings served as fecal markers by imparting a distinct color to each cat s feces, allowing identification in the litter box. In addition, colored glitter (1/8 teaspoon in canned food) was fed to cats and found to be a reliable fecal marker. Long-term feeding of colorings and glitter was found to be safe and effective at yielding readily identifiable stools.
Address Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Alabama 36841, USA
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Series Volume Series Issue Edition
ISSN 1060-0558 ISBN Medium
Area Expedition Conference
Notes PMID:11958604 Approved no
Call Number Equine Behaviour @ team @ Serial 4165
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Author Nowlan, S.S.; Deibel, R.H.
Title Group Q streptococci. I. Ecology, serology, physiology, and relationship to established enterococci Type Journal Article
Year 1967 Publication Journal of Bacteriology Abbreviated Journal J Bacteriol
Volume 94 Issue 2 Pages 291-296
Keywords Animals; Antigens/analysis; Cattle; Dogs; Enterococcus faecalis/metabolism; Feces/microbiology; Horses; Humans; Poultry; Precipitin Tests; Rabbits; Sheep; *Streptococcus/immunology/isolation & purification/metabolism; Swine
Abstract (down) The group Q streptococci possess unique serological and physiological characteristics which differentiate them from established enterococci. The group Q antigen was not demonstrable in all strains; however, all possessed the group D antigen. All group Q strains were physiologically similar regardless of whether or not they possessed the group Q antigen. These strains differed from the established enterococcal species, as they neither hydrolyzed arginine nor initiated growth in 1.0% methylene blue-milk. They also differed radically in the fermentation of various carbohydrates, especially the polyhydric sugar alcohols. The results indicate that the group Q streptococci constitute a unique taxonomic entity; the species designation Streptococcus avium sp. n. is suggested, owing to their characteristic occurrence in chicken fecal specimens.
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ISSN 0021-9193 ISBN Medium
Area Expedition Conference
Notes PMID:4962699 Approved no
Call Number Equine Behaviour @ team @ Serial 2746
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Author Alexander, F.
Title The effect of some anti-diarrhoeal drugs on intestinal transit and faecal excretion of water and electrolytes in the horse Type Journal Article
Year 1978 Publication Equine veterinary journal Abbreviated Journal Equine Vet J
Volume 10 Issue 4 Pages 229-234
Keywords Animals; Antidiarrheals/*pharmacology; Atropine/pharmacology; Electrolytes/*analysis/urine; Feces/*analysis; Gastrointestinal Motility/*drug effects; Horses/*metabolism/physiology; Loperamide/pharmacology; Male; Meperidine/pharmacology; Morphine/pharmacology; Opium/pharmacology; Water/*analysis
Abstract (down) The effect of morphine, Tinct. opii, loperamide, pethidine and atropine on intestinal transit and the faecal and urinary excretion of water and electrolytes was studied in ponies. The rate of passage of a particulate marker was slowed by morphine, hastened then slowed by loperamide and Tinct. opii, and hastened by atropine. The liquid marker was slowed by Tinct. opii and hastened then slowed by the other drugs. Only loperamide decreased the faecal sodium excretion. This drug also decreased faecal water and weight; it appeared worthy of clinical trial in diarrhoea. Tinct. opii decreased by morphine, pethidine and atropine increased faecal water.
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ISSN 0425-1644 ISBN Medium
Area Expedition Conference
Notes PMID:738263 Approved no
Call Number refbase @ user @ Serial 110
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Author Takai, S.; Narita, K.; Ando, K.; Tsubaki, S.
Title Ecology of Rhodococcus (Corynebacterium) equi in soil on a horse-breeding farm Type Journal Article
Year 1986 Publication Veterinary Microbiology Abbreviated Journal Vet Microbiol
Volume 12 Issue 2 Pages 169-177
Keywords Actinomycetales/classification/*growth & development; Animals; Corynebacterium/classification/*growth & development; Feces/microbiology; Female; Horses; Serotyping; *Soil Microbiology
Abstract (down) The ecology of Rhodococcus (Corynebacterium) equi in soil was studied on a horse-breeding farm. R. equi was cultured from soil at a depth of 0, 10, and 20 cm on the six sites of the farm at monthly intervals for 10 months from March to December of 1983. The highest numbers of R. equi were found in the surface soil. The mean number of bacteria in soil samples at every depth increased remarkably from 0 or 10(2) to 10(4) colony-forming units (CFU) g-1 of soil in the middle of April, and later decreased gradually. R. equi inoculated into six soil exudate broths prepared from surface soils at separate sites yielded suspensions with different optical densities, indicating differences in growth. The distribution of serotypes in the soil was similar to that in the horses on the farm. These findings indicated that R. equi could multiply in the soil and flourish in the cycle existing between horses and their soil environment.
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ISSN 0378-1135 ISBN Medium
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Notes PMID:3750818 Approved no
Call Number Equine Behaviour @ team @ Serial 2683
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Author Baltic, M.; Jenni-Eiermann, S.; Arlettaz, R.; Palme, R.
Title A noninvasive technique to evaluate human-generated stress in the black grouse Type Journal Article
Year 2005 Publication Annals of the New York Academy of Sciences Abbreviated Journal Ann N Y Acad Sci
Volume 1046 Issue Pages 81-95
Keywords Adrenocorticotropic Hormone/metabolism; Animals; Bird Diseases/*metabolism; Conservation of Natural Resources; Corticosterone/*metabolism; Ecosystem; Feces/*chemistry; Female; Galliformes/*metabolism; Immunoenzyme Techniques/methods/veterinary; Male; Reproducibility of Results; Stress/metabolism/*veterinary; Tritium/diagnostic use
Abstract (down) The continuous development of tourism and related leisure activities is exerting an increasingly intense pressure on wildlife. In this study, a novel noninvasive method for measuring stress in the black grouse, an endangered, emblematic species of European ecosystems that is currently declining in several parts of its European range, is tested and physiologically validated. A radiometabolism study and an ACTH challenge test were performed on four captive black grouse (two of each sex) in order to get basic information about the metabolism and excretion of corticosterone and to find an appropriate enzyme-immunoassay (EIA) to measure its metabolites in the feces. Peak radioactivity in the droppings was detected within 1 to 2 hours. Injected (3)H-corticosterone was excreted as polar metabolites and by itself was almost absent. A cortisone-EIA was chosen from among seven tested EIAs for different groups of glucocorticoid metabolites, because it cross-reacted with some of the formed metabolites and best reflected the increase of excreted corticosterone metabolites, after the ACTH challenge test. Concentrations of the metabolites from fecal samples collected from snow burrows of free-ranging black grouse were within the same range as in captive birds. The noninvasive method described may be appropriate for evaluating the stress faced by free-living black grouse populations in the wild, particularly in mountain ecosystems where human disturbance, especially by winter sports, is of increasing conservation concern.
Address Zoological Institute, Division of Conservation Biology, Baltzerstrasse 6, CH-3012 Bern, Switzerland
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Language English Summary Language Original Title
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ISSN 0077-8923 ISBN Medium
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Notes PMID:16055845 Approved no
Call Number Equine Behaviour @ team @ Serial 4080
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Author Kralj-Fiser, S.; Scheiber, I.B.R.; Blejec, A.; Moestl, E.; Kotrschal, K.
Title Individualities in a flock of free-roaming greylag geese: behavioral and physiological consistency over time and across situations Type Journal Article
Year 2007 Publication Hormones and Behavior Abbreviated Journal Horm Behav
Volume 51 Issue 2 Pages 239-248
Keywords Animals; Behavior, Animal/*physiology; Corticosterone/metabolism; Feces; Female; Geese/*physiology; Individuality; Male; Personality/*physiology; Population Density; Reproducibility of Results; Seasons; *Social Behavior; Social Environment; Testosterone/*metabolism
Abstract (down) The concept of personality implies individual differences in behavior and physiology that show some degree of repeatability/consistency over time and across contexts. Most studies of animal personality, particularly studies of individuals' variation in physiological mechanisms, have been conducted on selected individuals in controlled conditions. We attempted to detect consistent behaviors as well as physiological patterns in greylag ganders (Anser anser) from a free-roaming flock living in semi-natural conditions. We tested 10 individuals repeatedly, in a handling trial, resembling tests for characterization of “temperaments” in captive animals. We recorded the behavior of the same 10 individuals during four situations in the socially intact flock: (1) a “low density feeding condition”, (2) a “high density feeding condition”, (3) a “low density post-feeding situation” and (4) while the geese rested. We collected fecal samples for determination of excreted immuno-reactive corticosterone (BM) and testosterone metabolites (TM) after handling trials, as well as the “low density feeding” and the “high density feeding” conditions. BM levels were very highly consistent over the repeats of handling trials, and the “low density feeding condition” and tended to be consistent over the first two repeats of the “high density feeding condition”. Also, BM responses tended to be consistent across contexts. Despite seasonal variation, there tended to be inter-test consistency of TM, which pointed to some individual differences in TM as well. Aggressiveness turned out to be a highly repeatable trait, which was consistent across social situations, and tended to correlate with an individual's resistance during handling trials. Also, “proximity to the female partner” and “sociability” – the average number of neighboring geese in a close distance while resting – were consistent. We conclude that aggressiveness, “affiliative tendencies” and levels of excreted corticosterone and testosterone metabolites may be crucial factors of personality in geese.
Address University of Ljubljana, Biotechnical faculty, Slovenia. simona.kralj@guest.arnes.si
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ISSN 0018-506X ISBN Medium
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Notes PMID:17196200 Approved no
Call Number Equine Behaviour @ team @ Serial 4189
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Author Thiel, D.; Jenni-Eiermann, S.; Palme, R.
Title Measuring corticosterone metabolites in droppings of capercaillies (Tetrao urogallus) Type Journal Article
Year 2005 Publication Annals of the New York Academy of Sciences Abbreviated Journal Ann N Y Acad Sci
Volume 1046 Issue Pages 96-108
Keywords Adrenocorticotropic Hormone/administration & dosage/analysis/metabolism; Animals; Circadian Rhythm; Corticosterone/administration & dosage/*analysis/*metabolism; Feces/*chemistry; Female; Freezing; Galliformes/*metabolism; Male; Reproducibility of Results; Sex Factors; Temperature; Time Factors; Tritium/diagnostic use
Abstract (down) The capercaillie (Tetrao urogallus), the largest grouse species in the world, is decreasing in numbers in major parts of its distribution range. Disturbances by human outdoor activities are discussed as a possible reason for this population decline. An indicator for disturbances is the increase of the glucocorticoid corticosterone, a stress hormone, which helps to cope with life-threatening situations. However, repeated disturbances might result in a long-term increase of the basal corticosterone concentration, which can result in detrimental effects like reduced fitness and survival of an animal. To measure corticosterone metabolites (CMs) noninvasively in the droppings of free-living capercaillies, first an enzyme immunoassay (EIA) in captive birds had to be selected and validated. Therefore, the excretion pattern of intravenously injected radiolabeled corticosterone was determined and 3H metabolites were characterized. High-performance liquid chromatography (HPLC) separations of the samples containing peak concentrations revealed that corticosterone was extensively metabolized. The HPLC fractions were tested in several EIAs for glucocorticoid metabolites. The physiological relevance of this method was proved after pharmacological stimulation of the adrenocortical activity. Only the recently established cortisone assay, measuring CMs with a 3,11-dione structure, detected an expressed increase of concentrations following ACTH stimulation. To set up a sampling protocol suited for the field, we examined the influence of various storage conditions and time of day on concentrations of CMs.
Address Swiss Ornithological Institute, 6204 Sempach, Switzerland. dominik.thiel@vogelwarte.ch
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Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0077-8923 ISBN Medium
Area Expedition Conference
Notes PMID:16055846 Approved no
Call Number Equine Behaviour @ team @ Serial 4079
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