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Author Passler, S.; Pfeffer, M. openurl 
  Title (up) Detection of antibodies to alphaviruses and discrimination between antibodies to eastern and western equine encephalitis viruses in rabbit sera using a recombinant antigen and virus-specific monoclonal antibodies Type Journal Article
  Year 2003 Publication Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health Abbreviated Journal J Vet Med B Infect Dis Vet Public Health  
  Volume 50 Issue 6 Pages 265-269  
  Keywords Animals; Antibodies, Monoclonal/*immunology; Antibodies, Viral/*analysis/blood; DNA Primers; Encephalitis Virus, Eastern Equine/genetics/*immunology; Encephalitis Virus, Western Equine/genetics/*immunology; Encephalomyelitis, Equine/*diagnosis/*virology; Epitopes; Fluorescent Antibody Technique/*veterinary; Horses; Rabbits; Recombination, Genetic; Reverse Transcriptase Polymerase Chain Reaction/veterinary  
  Abstract Three arthropod-borne alphaviruses, western equine encephalitis viruses (WEEV), eastern equine encephalitis viruses (EEEV) and Venezuelan equine encephalitis viruses are the aetiological agents of a sometimes severe encephalomyelitis in equines and humans in the New World. With regard to the different ecology and epidemiology of these viruses, a method applied in serological screening should be able to distinguish between them as well as other related members of the genus Alphavirus in the American continent. However, this has been hampered in the past by (a) the close antigenic relationship between alphaviruses in traditional serological assays, especially in the routinely used haemagglutination-inhibition, and (b) the need of biosafety level 3 facilities to grow the viral antigens. An epitope blocking assay using an EEEV glycoprotein E1-expressing recombinant Sindbis virus and virus-specific monoclonal antibodies (mAbs) binding to the E1 of EEEV (strain NJ/60) and the E1 of Sindbis virus was established using automated flow cytometry. The test was evaluated using sera of infected and vaccinated rabbits. A cut-off value of 30% inhibition for antigenic complex-specific seroconversion was found to be sufficient for the detection of the respective infection. By using three different mAbs in parallel, we were able to detect alphavirus genus-, EEEV- and WEEV-complex-specific serum antibodies. As this test is based on the inhibition of binding of virus-specific mAbs, sera of every origin other than mouse can be tested. Thus, this assay may prove useful in the serological screening of a variety of animal species during an outbreak investigation.  
  Address Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0931-1793 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:14628996 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2639  
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Author Dyson, S.; Berger, J.; Ellis, A.D.; Mullard, J. url  doi
openurl 
  Title (up) Development of an ethogram for a pain scoring system in ridden horses and its application to determine the presence of musculoskeletal pain Type Journal Article
  Year 2018 Publication Journal of Veterinary Behavior Abbreviated Journal  
  Volume 23 Issue Pages 47-57  
  Keywords Lameness; Equine behavior; Pain grading; Headshaking; Bucking; Rearing  
  Abstract There is evidence that more than 47% of the sports horse population in normal work may be lame, but the lameness is not recognized by owners or trainers. An alternative means of detecting pain may be recognition of behavioral changes in ridden horses. It has been demonstrated that there are differences in facial expressions in nonlame and lame horses. The purpose of this study was to develop a whole horse ethogram for ridden horses and to determine whether it could be applied repeatedly by 1 observer (repeatability study, 9 horses) and if, by application of a related pain behavior score, lame horses (n = 24) and nonlame horses (n = 13) could be differentiated. It was hypothesized that there would be some overlap in pain behavior scores among nonlame and lame horses; and that overall, nonlame horses would have a lower pain behavior score than lame horses. The ethogram was developed with 117 behavioral markers, and the horses were graded twice in random order by a trained specialist using video footage. Overall, there was a good correlation between the 2 assessments (P < 0.001; R2 = 0.91). Behavioral markers that were not consistent across the 2 assessments were omitted, reducing the ethogram to 70 markers. The modified ethogram was applied to video recordings of the nonlame horses and lame horses (ethogram evaluation). There was a strong correlation between 20 behavioral markers and the presence of lameness. The ethogram was subsequently simplified to 24 behavioral markers, by the amalgamation of similar behaviors which scored similarly and by omission of markers which showed unreliable results in relation to lameness. Following this, the maximum individual occurrence score for lame horses was 14 (out of 24 possible markers), with a median and mean score of 9 (±2 standard deviation) compared with a maximum score of 6 for nonlame horses, with a median and mean score of 2 (±1.4). For lame horses, the following behaviors occurred significantly more (P < 0.05, chi-square): ears back, mouth opening, tongue out, change in eye posture and expression, going above the bit, head tossing, tilting the head, unwillingness to go, crookedness, hurrying, changing gait spontaneously, poor quality canter, resisting, and stumbling and toe dragging. Recognition of these features as potential indicators of musculoskeletal pain may enable earlier recognition of lameness and avoidance of punishment-based training. Further research is necessary to verify this new ethogram for assessment of pain in ridden horses.  
  Address  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1558-7878 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6706  
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Author Nagy, K.; Bodó, G.; Bárdos, G.; Bánszky, N.; Kabai, P. url  doi
openurl 
  Title (up) Differences in temperament traits between crib-biting and control horses Type Journal Article
  Year 2010 Publication Applied Animal Behaviour Science Abbreviated Journal Appl. Anim. Behav. Sci.  
  Volume 122 Issue 1 Pages 41-47  
  Keywords Crib-biting; Stereotypic behaviour; Equine temperament; Trainability; Coping strategies  
  Abstract Recent studies have suggested that crib-biting in horses is associated with diminished capacity of learning or coping with stress. Such findings raise the question whether trainability, which is fundamentally important in practice, could also be affected by stereotypic behaviour. Trainability of a horse is difficult to assess in simple tests, however, it is reliably estimated by experienced riders. To assess trainability and other characteristics related to that, a questionnaire survey was conducted with the owners of 50 crib-biting and 50 control horses. Where possible, control horses were selected from the same establishment as crib-biters. Groups did not differ significantly regarding age, breed, gender, training level or usage. Principal component analysis revealed three main factors which can be labelled as [`]Anxiety', [`]Affability' and [`]Trainability'. The [`]Anxiety' factor consisted of the items [`]Nervousness', [`]Excitability', [`]Panic', [`]Inconsistent emotionality', [`]Vigilance', [`]Skittishness', and [`]Timidity'. [`]Affability' consisted of [`]Friendliness toward people', [`]Cooperation', [`]Docility' and [`]Friendliness toward horses'. [`]Trainability' involved [`]Concentration', [`]Trainability', [`]Memory', and [`]Perseverance'. Temperament traits were not affected by age, gender, breed or training level, but the usage of the horse and the presence of crib-biting behaviour had significant effects. Competition horses had lower level of [`]Anxiety' (p = 0.032) and higher level of [`]Trainability' (p = 0.068) than leisure horses. Crib-biting horses had significantly lower level of [`]Anxiety' than control horses (p < 0.001), while [`]Trainability' and [`]Affability' did not differ between groups (p = 0.823 and p = 0.543, respectively). Competition horses are more often exposed to novel environment and to frightening stimuli (e.g. colourful obstacles) than leisure horses and therefore might have also become more habituated to these types of stimuli. Coping with novel situation may be enhanced by defusing nervous behaviour by the more experienced riders of competition. Previous studies indicated crib-biting horses to be less reactive when challenged as compared to control horses. We suggest that the virtual calmness and lower nervousness of the crib-biting horses might be due to the passive coping style of these animals. [`]Affability' of horses might be more related to housing and management conditions than to crib-biting. Contrary to expectations, scores on [`]Trainability' had not coincided with the impaired learning of crib-biting horses reported in laboratory tests. However, previous behavioural tests on equine learning rarely had a direct relevance to the training abilities of the horses. Our results do not support crib-biting stereotypy to affect performance in training, which is a complex learning process involving cooperation and docility in the social environment.  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0168-1591 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 5078  
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Author Scherer, W.F.; Dickerman, R.W.; Ordonez, J.V. openurl 
  Title (up) Discovery and geographic distribution of Venezuelan encephalitis virus in Guatemala, Honduras, and British Honduras during 1965-68, and its possible movement to Central America and Mexico Type Journal Article
  Year 1970 Publication The American Journal of Tropical Medicine and Hygiene Abbreviated Journal Am J Trop Med Hyg  
  Volume 19 Issue 4 Pages 703-711  
  Keywords Animals; Antibodies/analysis; Belize; Central America; Complement Fixation Tests; Cricetinae; Culicidae; *Disease Reservoirs; Ecology; Encephalitis Viruses/isolation & purification; Encephalomyelitis, Equine/*epidemiology; Guatemala; Hemagglutination Inhibition Tests; Honduras; Horses; Humans; Mexico; Neutralization Tests; Rats; Sampling Studies; Swine; Tropical Climate  
  Abstract  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9637 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:4393224 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2735  
Permanent link to this record
 

 
Author Yarnell, K.; Hall, C.; Royle, C.; Walker, S.L. url  doi
openurl 
  Title (up) Domesticated horses differ in their behavioural and physiological responses to isolated and group housing Type Journal Article
  Year 2015 Publication Physiology & Behavior Abbreviated Journal Physiol.Behav.  
  Volume 143 Issue Pages 51-57  
  Keywords Equine; Behaviour; Corticosterone; Housing  
  Abstract Abstract The predominant housing system used for domestic horses is individual stabling; however, housing that limits social interaction and requires the horse to live in semi-isolation has been reported to be a concern for equine welfare. The aim of the current study was to compare behavioural and physiological responses of domestic horses in different types of housing design that provided varying levels of social contact. Horses (n = 16) were divided equally into four groups and exposed to each of four housing treatments for a period of five days per treatment in a randomized block design. The four housing treatments used were single housed no physical contact (SHNC), single housed semi-contact (SHSC), paired housed full contact (PHFC) and group housed full contact (GHFC). During each housing treatment, adrenal activity was recorded using non-invasive faecal corticosterone metabolite analysis (fGC). Thermal images of the eye were captured and eye temperature was assessed as a non-invasive measure of the stress response. Behavioural analysis of time budget was carried out and an ease of handling score was assigned to each horse in each treatment using video footage. SHNC horses had significantly higher (p = 0.01) concentrations of fGC and were significantly (p = 0.003) more difficult to handle compared to the other housing types. GHFC horses, although not significantly different, had numerically lower concentrations of fGC and were more compliant to handling when compared to all other housing treatments. Eye temperature was significantly (p = 0.0001) lower in the group housed treatment when compared to all other treatments. These results indicate that based on physiological and behavioural measures incorporating social contact into the housing design of domestic horses could improve the standard of domestic equine welfare.  
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  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0031-9384 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 5920  
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Author Giles, S.L.; Nicol, C.J.; Harris, P.A.; Rands, S.A. url  doi
openurl 
  Title (up) Dominance rank is associated with body condition in outdoor-living domestic horses (Equus caballus) Type Journal Article
  Year 2015 Publication Applied Animal Behaviour Science Abbreviated Journal  
  Volume Issue Pages  
  Keywords Equine; fatness; obesity; social behaviour; displacement  
  Abstract Abstract The aim of our study was to explore the association between dominance rank and body condition in outdoor group-living domestic horses, Equus caballus. Social interactions were recorded using a video camera during a feeding test, applied to 203 horses in 42 herds. Dominance rank was assigned to 194 individuals. The outcome variable body condition score (BCS) was recorded using a 9-point scale. The variables age and height were recorded and considered as potential confounders or effect modifiers. Results were analysed using multivariable linear and logistic regression techniques, controlling for herd group as a random effect. More dominant (p = 0.001) individuals generally had a higher body condition score (p = 0.001) and this association was entirely independent of age and height. In addition, a greater proportion of dominant individuals fell into the obese category (BCS >= 7/9, p = 0.005). There were more displacement encounters and a greater level of interactivity in herds that had less variation in age and height, lending strength to the hypothesis that phenotypic variation may aid cohesion in group-living species. In addition there was a strong quadratic relationship between age and dominance rank (p < 0.001), where middle-aged individuals were most likely to be dominant. These results are the first to link behavioural predictors to body condition and obesity status in horses and should prompt the future consideration of behavioural and social factors when evaluating clinical disease risk in group-living animals.  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0168-1591 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Giles2015 Serial 5864  
Permanent link to this record
 

 
Author Gibbs, P.G.; Cohen, N.D. openurl 
  Title (up) Early management of race-bred weanlings and yearlings on farms Type Journal Article
  Year 2001 Publication Journal of Equine Veterinary Science Abbreviated Journal J. Equine Vet. Sci.  
  Volume 21 Issue 6 Pages 279-283  
  Keywords Equine, management, growth, nutrition, marketing  
  Abstract A total of 58 Thoroughbred and Quarter Horse farms

that managed 1,987 weanlings and yearlings responded to

a survey designed to better characterize early management

of racing prospects. Average age at weaning was 5.5 months

and over half of all farms kept almost three-fourths of all

weanlings to be placed in pre-race training. Variation in

feeding practices was evident and while well over half

of all farms provided balanced nutrient supply to young

horses, 20% to 40% likely fed unbalanced diets. An obvious

preference existed for semi-confinement in young horses

with plenty of free exercise. The majority of farms reported

that young prospects were fed and managed for a moderate

rate of growth. Forced exercise occurred to a much larger

extent with yearlings than weanlings and 40% of farms

described the footing as soft, but not deep. Response to the

prevalence of developmental orthopedic diseases appeared

somewhat guarded, and average injury rate was low on

farms that attributed much of injury to horses playing too

hard. Technological advancements such as photoperiod

manipulation in broodmares were widely used, while

valuable tools such as body condition scoring were utilized

to a lesser extent.
 
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  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 5758  
Permanent link to this record
 

 
Author Scherer, W.F.; Madalengoitia, J.; Flores, W.; Acosta, M. openurl 
  Title (up) Ecologic studies of Venezuelan encephalitis virus in Peru during 1970-1971 Type Journal Article
  Year 1975 Publication American Journal of Epidemiology Abbreviated Journal Am J Epidemiol  
  Volume 101 Issue 4 Pages 347-355  
  Keywords Animals; Antibodies, Viral; Cricetinae/immunology; Culicidae/microbiology; *Disease Vectors; Ecology; *Encephalitis Virus, Venezuelan Equine/immunology/isolation & purification; Encephalomyelitis, Equine/immunology/microbiology/transmission; Female; Hemagglutination Inhibition Tests; Horses/immunology; Humans; Neutralization Tests; Peru  
  Abstract Venezuelan encephalitis (VE) virus has intermittently produced epidemics and equine epizootics on the dry Pacific coastal plain of Peru since at least the 1930's. However, evidence that the virus exists in the Amazon region of Peru to the east of the Andes mountains was not obtained until antibodies were found in human sera collected in 1965, and 10 strains of the virus were isolated in a forest near the city of Iquitos, Peru during February and March 1971. Eight strains came from mosquitoes and two from dead sentinel hamsters. Three hamsters exposed in forests near Iquitos developed VE virus antibodies suggesting that hamster-benign strains also exist there. Antibody tests of equine sera revealed no evidence that VE virus was actively cycling during the late 1950's or 1960's in southern coastal Peru, where equine epizootics had occurred in the 1930's and 1940's. In northern coastal Peru bordering Ecuador, antibodies were present in equine sera, presumably residual from the 1969 outbreak caused by subtype I virus, since neutralizing antibody titers were higher to subtype I virus than to subtypes III or IV. No VE virus was detected in this northern region during the dry season of 1970 by use of sentinel hamsters. The possibility is considered that VE epidemics and equine epizootics on the Pacific coast of Peru are caused by movements of virus in infected vertebrates traversing Andean passes or in infected vertebrates or mosquitoes carried in airplanes from the Amazon region.  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9262 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:235838 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2705  
Permanent link to this record
 

 
Author Scherer, W.F.; Dickerman, R.W. openurl 
  Title (up) Ecologic studies of Venezuelan encephalitis virus in southeastern Mexico. 8. Correlations and conclusions Type Journal Article
  Year 1972 Publication The American Journal of Tropical Medicine and Hygiene Abbreviated Journal Am J Trop Med Hyg  
  Volume 21 Issue 2 Pages 86-89  
  Keywords Animals; Birds; Cattle; Chiroptera; Cricetinae; Culex; Culicidae; *Disease Reservoirs; Ecology; Encephalitis Virus, Venezuelan Equine/immunology; Encephalitis Viruses/*isolation & purification; Encephalomyelitis, Equine/epidemiology/*etiology; Horses; Humans; *Insect Vectors; Mammals; Mexico; Mice; Opossums; Rats; Swine  
  Abstract  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9637 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:4399844 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2721  
Permanent link to this record
 

 
Author Chaplin, S.J.; Gretgrix, L. url  doi
openurl 
  Title (up) Effect of housing conditions on activity and lying behaviour of horses Type Journal Article
  Year 2010 Publication Abbreviated Journal animal  
  Volume 4 Issue 5 Pages 792-795  
  Keywords lying, behaviour, activity, equine, housing  
  Abstract Housing conditions for horses impose various levels of confinement, which may compromise welfare. Lying behaviour and activity can be used as welfare indicators for domestic animals and rebound behaviour suggests a build-up of motivation resulting from deprivation. The objective of this study was to determine if activity and lying behaviour of horses are affected by housing conditions and to investigate the occurrence of rebound behaviour after release from confinement. Eight horses were subjected, in pairs, to each of four experimental treatments; paddock (P), fully stabled (FS), partly stabled (PS) and yard (Y). Each horse received 6 days acclimatisation prior to the 24 h recording period. Time spent in lying and activity were electronically recorded using a tilt switch and motion sensor connected to a data logger worn on the horse's left foreleg. Time spent active during the first 5 min of release from stable to paddock in the PS treatment (days 1 and 5) and at the same time of day in the P treatment was used as a measure of rebound behaviour. Effect of housing conditions on total time spent active was highly significant (FS = 123 s, PS = 158 s, Y = 377 s, P = 779 s, P < 0.001). Housing conditions did not significantly affect total time spent lying (P = 0.646). Horses were significantly more active, compared with baseline paddock behaviour, on release from stabling on both days 1 (P = 0.006) and 5 (P = 0.025) of PS treatment. These results suggest that activity patterns of horses, but not lying behaviour, are affected by the housing conditions tested and that rebound activity occurs in horses after a period of confinement.  
  Address  
  Corporate Author Thesis  
  Publisher Cambridge University Press Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition 2010/01/21  
  ISSN 1751-7311 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6603  
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