| |
Records |
Links |
|
Author |
McGreevy, P.D.; Nicol, C.J. |
|
| |
Title |
The effect of short-term prevention on the subsequent rate of crib-biting in thoroughbred horses |
Type |
Journal Article |
| |
Year |
1998 |
Publication |
Equine veterinary journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
|
| |
Volume |
|
Issue |
27 |
Pages |
30-34 |
|
| |
Keywords |
Analysis of Variance; Animals; *Behavior, Animal; Horse Diseases/*prevention & control/psychology; Horses; Male; Recurrence; *Stereotyped Behavior; Videotape Recording |
|
| |
Abstract |
The results of an experimental study of the motivational consequences of short-term prevention of crib-biting are reported here. Eight test horses wore a cribbing collar for 24 h. This was effective in preventing crib-biting in 6 subjects. Using analysis of co-variance that accounted for baseline differences in crib-biting rate, test horses showed significantly more crib-biting than control horses on the first day after prevention (P < 0.05). There was also a highly significant increase in the crib-biting rate of test horses on the first day after prevention in comparison with their baseline rate (P < 0.01). This defines the increase as a post inhibitory rebound. An increase in the novelty of the cribbing bar and an increase in feeding motivation during the period of prevention are rejected as explanations of the rebound in this study. Instead, it is suggested that the rebound reflected a rise in internal motivation to crib-bite during the period of prevention. Behaviours that exhibit this pattern of motivation are generally considered functional; and it has been argued that their prevention may compromise welfare. |
|
| |
Address |
Department of Clinical Veterinary Science, University of Bristol, UK |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN  |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:10485001 |
Approved |
no |
|
| |
Call Number |
refbase @ user @ |
Serial |
88 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Clayton, H.M. |
|
| |
Title |
Classification of collected trot, passage and piaffe based on temporal variables |
Type |
Journal Article |
| |
Year |
1997 |
Publication |
Equine Veterinary Journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
|
| |
Volume |
|
Issue |
23 |
Pages |
54-57 |
|
| |
Keywords |
Analysis of Variance; Animals; Discriminant Analysis; Gait/*physiology; Hindlimb/physiology; Horses/*physiology; Locomotion/*physiology; Male; Running/physiology; Time Factors; Videotape Recording; Walking/physiology |
|
| |
Abstract |
The objective was to determine whether collected trot, passage and piaffe could be distinguished as separate gaits on the basis of temporal variables. Sagittal plane, 60 Hz videotapes of 10 finalists in the dressage competitions at the 1992 Olympic Games were analysed to measure the temporal variables in absolute terms and as percentages of stride duration. Classification was based on analysis of variance, a graphical method and discriminant analysis. Stride duration was sufficient to distinguish collected trot from passage and piaffe in all horses. The analysis of variance showed that the mean values of most variables differed significantly between passage and piaffe. When hindlimb stance percentage was plotted against diagonal advanced placement percentage, some overlap was found between all 3 movements indicating that individual horses could not be classified reliably in this manner. Using hindlimb stance percentage and diagonal advanced placement percentage as input in a discriminant analysis, 80% of the cases were classified correctly, but at least one horse was misclassified in each movement. When the absolute, rather than percentage, values of the 2 variables were used as input in the discriminant analysis, 90% of the cases were correctly classified and the only misclassifications were between passage and piaffe. However, the 2 horses in which piaffe was misclassified as passage were the gold and silver medallists. In general, higher placed horses tended toward longer diagonal advanced placements, especially in collected trot and passage, and shorter hindlimb stance percentages in passage and piaffe. |
|
| |
Address |
Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:9354290 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
3738 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Barrey, E.; Galloux, P. |
|
| |
Title |
Analysis of the equine jumping technique by accelerometry |
Type |
Journal Article |
| |
Year |
1997 |
Publication |
Equine Veterinary Journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
|
| |
Volume |
|
Issue |
23 |
Pages |
45-49 |
|
| |
Keywords |
*Acceleration; Analysis of Variance; Animals; Forelimb/physiology; Hindlimb/physiology; Horses/*physiology; Locomotion/*physiology; Movement/physiology; Time Factors |
|
| |
Abstract |
The purpose of this study was to demonstrate the relationships between jumping technique and dorsoventral acceleration measured at the sternum. Eight saddle horses of various jumping abilities competed on a selective experimental show jumping course including 14 obstacles. An accelerometric belt fastened onto the thorax continuously measured the dorsoventral acceleration during the course. At each jump, 11 locomotor parameters (acceleration peaks, durations and stride frequency) were obtained from the dorsoventral acceleration-time curves. The type of obstacle significantly influenced the hindlimb acceleration peak at take-off and the landing acceleration peak (P<0.01). The poor jumpers exhibited a higher mean forelimb acceleration peak at take-off, a higher forelimb/hindlimb ratio between peaks of acceleration (F/H), and a lower approach stride frequency than good jumpers. Knocking over an obstacle was significantly associated with a low hindlimb acceleration peak at take-off and a high F/H ratio (P<0.01). In order to observe the continuous changes in the frequency domain of the dorsoventral acceleration during the approach and take-off phase, a Morlet's wavelet analysis was computed for each horse jumping over a series of 3 vertical obstacles. Different patterns of time-frequency images obtained by wavelet analysis were found when the horse either knocked over a vertical obstacle or cleared it. In the latter case, the image pattern showed an instantaneous increase in stride frequency at the end of the approach phase, and a marked energy content in the middle frequency range at take-off. |
|
| |
Address |
INRA Station de Genetique Quantitative et Appliquee, Groupe cheval, Jouy-en-Josas, France |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:9354288 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
3796 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Murray, J.K.; Senior, J.M.; Singer, E.R. |
|
| |
Title |
A comparison of cross-country recovery rates at CCI 2* with and without steeplechase competitions |
Type |
Journal Article |
| |
Year |
2006 |
Publication |
Equine Veterinary Journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
|
| |
Volume |
|
Issue |
36 |
Pages |
133-138 |
|
| |
Keywords |
Analysis of Variance; Animals; Area Under Curve; Blood Chemical Analysis/veterinary; Blood Gas Analysis/veterinary; Calcium/blood; Heart Rate/physiology; Hematocrit/veterinary; Horses/*blood/*physiology; Lactates/blood; Physical Conditioning, Animal/*methods/*physiology; Time Factors |
|
| |
Abstract |
REASONS FOR PERFORMING STUDY: Short format 3-day events were introduced in 2004. Anecdotal reports suggested that horses were more tired on completion of the cross-country phase of short format events when compared with horses completing the cross-country phase of long format competitions, despite the absence of Phases A, B and C. OBJECTIVES: To compare the physiological parameters and haematological parameters of horses that had completed the cross-country phase of a short format (SF) and a long format (LF) CCI 2* competition. METHODS: During a CCI 2* competition 69 competitors took part in the short format and 74 in the long format competition. Long format competitors completed Phases A, B, C and D and short format competitors completed Phase D only. Phase D (the cross-country course) was identical for both competitions. Two-way ANOVA for repeated measures and post hoc tests were used to compare temperature, pulse and respiration rates of horses competing in both types of competition. T tests were used to compare mean lactate and electrolyte concentrations, while U-Mann Whitney tests were used to compare CK and AST levels measured in horses competing in the short and long formats of the event. RESULTS: Training schedules, age and previous competition experience were not significantly different between horses competing in the SF and LF competitions. On completion of Phase D, SF horses had significantly higher PCV and significantly lower ionised calcium concentrations when compared with LF horses. LF horses had significantly higher heart rates than SF horses 10 min prior to starting Phase D and immediately after completing Phase D; however, no other significant differences were found between the 2 groups of horses. CONCLUSIONS: Only weak evidence was found to support the hypothesis that the workload for the horse in a SF CCI 2* competition is significantly different when compared to the LF CCI 2* competition. POTENTIAL RELEVANCE: There is no beneficial or detrimental effect on horses that complete short format CCI 2* competitions as compared to those that complete long format CCI 2* competitions but further research is required into the physiological response of horses at CCI 3* and CCI 4* short format competitions. |
|
| |
Address |
Department of Veterinary Clinical Science, University of Bristol, Langford House, Langford, Bristol, UK |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:17402408 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
4011 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Kingston, J.K.; Soppet, G.M.; Rogers, C.W.; Firth, E.C. |
|
| |
Title |
Use of a global positioning and heart rate monitoring system to assess training load in a group of thoroughbred racehorses |
Type |
Journal Article |
| |
Year |
2006 |
Publication |
Equine Veterinary Journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
|
| |
Volume |
|
Issue |
36 |
Pages |
106-109 |
|
| |
Keywords |
Analysis of Variance; Animals; Female; *Geographic Information Systems; Heart Rate/*physiology; Horses/*physiology; Male; Monitoring, Physiologic/veterinary; Physical Conditioning, Animal/*physiology; Satellite Communications; Telemetry/methods/*veterinary |
|
| |
Abstract |
REASONS FOR PERFORMING STUDY: Training is an important variable for determining athletic success. Nonetheless, there has been minimal scientific evaluation of racehorse training programmes. Training of racehorses focuses on running the horses at certain speeds using a combination of a stopwatch and rider's 'feel' for a horse's work intensity. Consequently, actual work intensity for individual horses is not clearly defined. OBJECTIVES: To 1) utilise a combined global positioning system (GPS) and heart rate monitor system to quantify training intensity and physiological responses of a group of racehorses undergoing training and racing; and 2) compare the workload measured by the GPS to that timed and recorded daily by a racehorse trainer. METHODS: Nineteen racehorses age 3 years were followed through a traditional training and racing programme over a 4 month period. Daily GPS and heart rate data together with the trainer's timing and distance data were collected while the horses were trained. Data were analysed using an ANOVA for repeated measures. RESULTS: The combined GPS/heart rate monitoring system detected different heart rate responses in individual horses subjected to the same training workouts. The average speeds detected with the GPS system were in agreement with average speeds timed by the trainer. However, peak speeds reached during training were significantly greater (P<0.05) than those estimated with stopwatch timing. The horses average training speeds increased significantly over the duration of the training period. CONCLUSIONS AND POTENTIAL RELEVANCE: The results from this study show that a GPS/heart rate monitor system provides a reliable measure of daily workload in horses during training. This technology provides a detailed picture of horses' training sessions and has the potential to provide a greater insight into the types of training that may predispose horses to injury. |
|
| |
Address |
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:17402402 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
4014 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Clayton, H.M. |
|
| |
Title |
Comparison of the stride kinematics of the collected, medium, and extended walks in horses |
Type |
Journal Article |
| |
Year |
1995 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
|
| |
Volume |
56 |
Issue |
7 |
Pages |
849-852 |
|
| |
Keywords |
Analysis of Variance; Animals; *Gait; Horses/*physiology; *Locomotion; Motion Pictures; Physical Conditioning, Animal; Walking |
|
| |
Abstract |
Six horses, highly trained for dressage competition, were used to study the stride kinematics of the walk, and to compare the kinematics of the collected, medium, and extended walks. Horses were filmed in a sagittal plane at a rate of 150 frames/s; temporal, linear, and angular data were extracted from the films. Results of ANOVA and Duncan's multiple range test indicated that the speed of the collected walk (1.37 m/s) was significantly (P < 0.01) slower than that of the medium (1.73 m/s) and extended (1.82 m/s) walks, values for which were not significantly different from each other. The increase in speed was associated with a significant increase in stride length, from 157 cm in the collected walk to 193 cm in the extended walk. This was a result of an increase in the over-tracking distance, whereas there was no significant difference in the distance between lateral placements of the limbs. Stride duration decreased (P < 0.01) from the collected walk (1,159 ms) to the extended walk (1,064 ms). Angles of the metacarpal and metatarsal segments, measured on the palmar/ plantar aspect, were higher at impact and lower at lift off in the collected than in the extended walk (P < 0.01). This indicated greater range of angular motion of this segment during the stance phase in the extended walk. Only 1 of the 6 horses had a regular 4-beat rhythm of the footfalls, with equal time elapsing between the lateral and diagonal footfalls. |
|
| |
Address |
Department of Veterinary Anatomy, Western College of Veterinary Medicine, Canada |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0002-9645 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:7574149 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
3743 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Santamaria, S.; Bobbert, M.E.; Back, W.; Barneveld, A.; van Weeren, P.R. |
|
| |
Title |
Variation in free jumping technique within and among horses with little experience in show jumping |
Type |
Journal Article |
| |
Year |
2004 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
|
| |
Volume |
65 |
Issue |
7 |
Pages |
938-944 |
|
| |
Keywords |
*Acceleration; Analysis of Variance; Animals; Biomechanics; Forelimb/physiology; Gait/*physiology; Hindlimb/physiology; Horses/*physiology; Locomotion/*physiology; Models, Biological; Video Recording |
|
| |
Abstract |
OBJECTIVE: To quantify variation in the jumping technique within and among young horses with little jumping experience, establish relationships between kinetic and kinematic variables, and identify a limited set of variables characteristic for detecting differences in jumping performance among horses. ANIMALS: Fifteen 4-year-old Dutch Warmblood horses. PROCEDURE: The horses were raised under standardized conditions and trained in accordance with a fixed protocol for a short period. Subsequently, horses were analyzed kinematically during free jumping over a fence with a height of 1.05 m. RESULTS: Within-horse variation in all variables that quantified jumping technique was smaller than variation among horses. However, some horses had less variation than others. Height of the center of gravity (CG) at the apex of the jump ranged from 1.80 to 2.01 m among horses; this variation could be explained by the variation in vertical velocity of the CG at takeoff (r, 0.78). Horses that had higher vertical velocity at takeoff left the ground and landed again farther from the fence, had shorter push-off phases for the forelimbs and hind limbs, and generated greater vertical acceleration of the CG primarily during the hind limb push-off. However, all horses cleared the fence successfully, independent of jumping technique. CONCLUSIONS AND CLINICAL RELEVANCE: Each horse had its own jumping technique. Differences among techniques were characterized by variations in the vertical velocity of the CG at takeoff. It must be determined whether jumping performance later in life can be predicted from observing free jumps of young horses. |
|
| |
Address |
Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 12, NL-3584 CM Utrecht, The Netherlands |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0002-9645 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:15281652 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
3772 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Santamaria, S.; Bobbert, M.F.; Back, W.; Barneveld, A.; van Weeren, P.R. |
|
| |
Title |
Effect of early training on the jumping technique of horses |
Type |
Journal Article |
| |
Year |
2005 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
|
| |
Volume |
66 |
Issue |
3 |
Pages |
418-424 |
|
| |
Keywords |
Age Factors; Analysis of Variance; Animals; Biomechanics; Forelimb/physiology; Gait/*physiology; Hindlimb/physiology; Horses/growth & development/*physiology; Locomotion/*physiology; Models, Biological; Physical Conditioning, Animal/*methods |
|
| |
Abstract |
OBJECTIVE: To investigate the effects of early training for jumping by comparing the jumping technique of horses that had received early training with that of horses raised conventionally. ANIMALS: 40 Dutch Warmblood horses. PROCEDURE: The horses were analyzed kinematically during free jumping at 6 months of age. Subsequently, they were allocated into a control group that was raised conventionally and an experimental group that received 30 months of early training starting at 6 months of age. At 4 years of age, after a period of rest in pasture and a short period of training with a rider, both groups were analyzed kinematically during free jumping. Subsequently, both groups started a 1-year intensive training for jumping, and at 5 years of age, they were again analyzed kinematically during free jumping. In addition, the horses competed in a puissance competition to test maximal performance. RESULTS: Whereas there were no differences in jumping technique between experimental and control horses at 6 months of age, at 4 years, the experimental horses jumped in a more effective manner than the control horses; they raised their center of gravity less yet cleared more fences successfully than the control horses. However, at 5 years of age, these differences were not detected. Furthermore, the experimental horses did not perform better than the control horses in the puissance competition. CONCLUSIONS AND CLINICAL RELEVANCE: Specific training for jumping of horses at an early age is unnecessary because the effects on jumping technique and jumping capacity are not permanent. |
|
| |
Address |
Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, NL-3584 CM Utrecht, The Netherlands |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0002-9645 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:15822585 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
4037 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Nicol, C.J.; Potzsch, C.; Lewis, K.; Green, L.E. |
|
| |
Title |
Matched concurrent case-control study of risk factors for feather pecking in hens on free-range commercial farms in the UK |
Type |
Journal Article |
| |
Year |
2003 |
Publication |
British poultry science |
Abbreviated Journal |
Br Poult Sci |
|
| |
Volume |
44 |
Issue |
4 |
Pages |
515-523 |
|
| |
Keywords |
*Aggression; Analysis of Variance; Animal Husbandry/methods; Animals; Case-Control Studies; Chickens/*physiology; Feathers; Female; Multivariate Analysis; Odds Ratio; Regression Analysis; Species Specificity |
|
| |
Abstract |
1. The aim of the study was to compare the management and husbandry of free-range flocks in the UK where feather pecking was either present (case) or absent (control). 2. One hundred flocks were enrolled into a concurrent case-control study: 50 where birds had recently started feather pecking, and 50 matched control flocks where birds of the same age had not started feather pecking. 3. Information was obtained from a detailed interview with the flock manager, and by direct inspection of the flock, house and range. 4. Initial univariate analyses revealed that case flocks were more likely to comprise ISA Brown than Lohmann, were more likely to be restricted from litter areas to prevent floor eggs, and were less likely to use the outside range. 5. Cluster analysis indicated that feather pecking was not associated with any particular husbandry system. 6. The only influential risk factor significant in the multivariable conditional logistic regression analysis was use of the outdoor range. The risk of feather pecking was reduced 9-fold in flocks where more than 20% of birds used the range on sunny days (odds ratio = 0.12). Use of the range was positively associated with the presence of trees and/or hedges on the range. |
|
| |
Address |
Department of Clinical Veterinary Science, University of Bristol, England. c.j.nicol@bris.ac.uk |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0007-1668 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:14584840 |
Approved |
no |
|
| |
Call Number |
refbase @ user @ |
Serial |
79 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Touma, C.; Palme, R.; Sachser, N. |
|
| |
Title |
Analyzing corticosterone metabolites in fecal samples of mice: a noninvasive technique to monitor stress hormones |
Type |
Journal Article |
| |
Year |
2004 |
Publication |
Hormones and Behavior |
Abbreviated Journal |
Horm Behav |
|
| |
Volume |
45 |
Issue |
1 |
Pages |
10-22 |
|
| |
Keywords |
Adrenal Cortex/drug effects; Adrenal Cortex Function Tests; Adrenocorticotropic Hormone/pharmacology; Analysis of Variance; Animals; Circadian Rhythm; Corticosterone/*analysis/metabolism; Dexamethasone/pharmacology; Feces/*chemistry; Female; Immunoenzyme Techniques/*methods; Male; Mice; Mice, Inbred C57BL; Models, Animal; Reproducibility of Results; Stress, Psychological/*metabolism |
|
| |
Abstract |
In small animals like mice, the monitoring of endocrine functions over time is constrained seriously by the adverse effects of blood sampling. Therefore, noninvasive techniques to monitor, for example, stress hormones in these animals are highly demanded in laboratory as well as in field research. The aim of our study was to evaluate the biological relevance of a recently developed technique to monitor stress hormone metabolites in fecal samples of laboratory mice. In total, six experiments were performed using six male and six female mice each. Two adrenocorticotropic hormone (ACTH) challenge tests, two dexamethasone (Dex) suppression tests and two control experiments [investigating effects of the injection procedure itself and the diurnal variation (DV) of glucocorticoids (GCs), respectively] were conducted. The experiments clearly demonstrated that pharmacological stimulation and suppression of adrenocortical activity was reflected accurately by means of corticosterone metabolite (CM) measurements in the feces of males and females. Furthermore, the technique proved sensitive enough to detect dosage-dependent effects of the ACTH/Dex treatment and facilitated to reveal profound effects of the injection procedure itself. Even the naturally occurring DV of GCs could be monitored reliably. Thus, our results confirm that measurement of fecal CM with the recently established 5alpha-pregnane-3beta,11beta,21-triol-20-one enzyme immunoassay is a very powerful tool to monitor adrenocortical activity in laboratory mice. Since mice represent the vast majority of all rodents used for research worldwide and the number of transgenic and knockout mice utilized as animal models is still increasing, this noninvasive technique can open new perspectives in biomedical and behavioral science. |
|
| |
Address |
Department of Behavioural Biology, University of Muenster, D-48149 Muenster, Germany. touma@uni-muenster.de |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0018-506X |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:14733887 |
Approved |
no |
|
| |
Call Number |
Equine Behaviour @ team @ |
Serial |
4084 |
|
| Permanent link to this record |
