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Title |
Horses' (Equus Caballus) Laterality, Stress Hormones, and Task Related Behavior in Innovative Problem-Solving |
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Equine Behaviour @ team @ ref3 |
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6572 |
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Author |
Griebenow, K.; Klibanov, A.M. |
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Title |
Lyophilization-induced reversible changes in the secondary structure of proteins |
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Journal Article |
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1995 |
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Proc Natl Acad Sci USA |
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92 |
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24 |
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10969-10976 |
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Changes in the secondary structure of some dozen different proteins upon lyophilization of their aqueous solutions have been investigated by means of Fourier-transform infrared spectroscopy in the amide III band region. Dehydration markedly (but reversibly) alters the secondary structure of all the proteins studied, as revealed by both the quantitative analysis of the second derivative spectra and the Gaussian curve fitting of the original infrared spectra. Lyophilization substantially increases the beta-sheet content and lowers the alpha-helix content of all proteins. In all but one case, proteins become more ordered upon lyophilization. |
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Equine Behaviour @ team @ |
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6519 |
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Author |
Palme, R.; Touma, C.; Arias, N., Dominchin, M.N.; Lepschy, M. |
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Title |
Steroid extraction: Get the best out of faecal samples |
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2013 |
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Wiener Tierärztliche Wochenschriften |
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Wien Tierärztl Monat – Vet Med Austria |
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100 |
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238-246. |
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Review, faeces, extraction, non-invasive hormone monitoring, stress, reproduction. |
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Faecal steroid hormone metabolites are becoming increasingly popular as parameters for reproductive functions and stress. The extraction of the steroids from the faecal matrix represents the initial step before quantification can be performed. The steroid metabolites present in the faecal matrix are of varying polarity and composition, so selection of a proper extraction procedure is essential. There have been some studies to address this complex but often neglected point. Radiolabelled steroids (e.g. cortisol or progesterone) have frequently been added to faecal samples to estimate the efficiency of the extraction procedures used. However, native, unmetabolized steroids are normally not present in the faeces and therefore the results are artifi- cial and do not accurately reflect the actual recoveries of the substances of interest. In this respect, recovery experiments based on faecal samples from radiometabolism studies are more informative. In these samples, the metabolite content accurately reflects the mixture of metabolites present in the given species. As a result, it is possible to evaluate different extraction methods for use with faecal samples. We present studies on sheep, horses, pigs, hares and dogs that utilized samples containing naturally metabolized, 14C-labelled steroids. We recommend extracting faecal steroids by simply suspending the faeces in a high percentage of a primary alcohol (for glucocorticoid metabolites 80% aqueous methanol proved best suited for virtually all mammalian species tested so far). Not only does the procedure significantly increase the total amount of recovered radioactivity, it also increases the percentage of unconjugated metabolites, which are more likely to be recognized by the antibodies used in various immunoassays. The advantages of this extraction procedure are clear: it is very easy to use (no evaporation step is needed), it yields high recoveries and variation based on the extraction procedure is reduced to a minimum. |
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Equine Behaviour @ team @ |
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6520 |
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Author |
Lee, P. |
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Title |
Adaptation to environmental change:an evolutionary perspective |
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Year |
1991 |
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Primate responses to environmental changes |
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39-56 |
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Chapmann & Hall |
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London |
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H. O. Box |
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Equine Behaviour @ team @ |
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6523 |
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Author |
Beck, B. B. |
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Title |
Animal tool behaviour: The use and manufacture of tools by animals |
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1980 |
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Garland |
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New York |
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Equine Behaviour @ team @ |
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6524 |
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Fisher, J.; Hinde, R. A. |
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Title |
The opening of milk bottles by birds |
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1994 |
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British Birds |
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British Birds |
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42 |
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347-357 |
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Equine Behaviour @ team @ |
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6525 |
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Kuczaj, S. A; Makecha, R.; Trone, M.; Paulos, R. D; Ramos, J. A. |
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Title |
Role of Peers in Cultural Innovation and Cultural Transmission: Evidence from the Play of Dolphin Calves |
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2006 |
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International Journal of Comparative Psychology |
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Int. J. Comp. Psychol |
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19 |
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2 |
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223-240 |
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Equine Behaviour @ team @ |
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6526 |
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Author |
Thorpe, W. H. |
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Title |
Learning and Instinct in Animals. |
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1963 |
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Methuen |
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London |
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Equine Behaviour @ team @ |
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6527 |
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Author |
Forrester, G.; Hudry, K.; Lindell, A.; Hopkins, W. D. |
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Title |
Cerebral Lateralization and Cognition: Evolutionary and Developmental Investigations of Behavioral Biases |
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2018 |
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238 |
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Academic Press |
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Cambridge |
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9780128146729 |
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Equine Behaviour @ team @ |
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6530 |
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Author |
Reader, S. M.; Laland, K.N. |
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Title |
Animal Innovation |
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2003 |
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Oxford University Press |
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Oxford |
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Equine Behaviour @ team @ |
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6531 |
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