| Records |
| Author |
Gulotta, M.; Gilmanshin, R.; Buscher, T.C.; Callender, R.H.; Dyer, R.B. |
| Title |
Core formation in apomyoglobin: probing the upper reaches of the folding energy landscape |
Type |
Journal Article |
| Year |
2001 |
Publication |
Biochemistry |
Abbreviated Journal |
Biochemistry |
Volume  |
40 |
Issue |
17 |
Pages |
5137-5143 |
| Keywords |
Animals; Apoproteins/*chemistry; Computer Simulation; Horses; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Myoglobin/*chemistry; *Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence/instrumentation/methods; Thermodynamics; Tryptophan/chemistry |
| Abstract |
An acid-destabilized form of apomyoglobin, the so-called E state, consists of a set of heterogeneous structures that are all characterized by a stable hydrophobic core composed of 30-40 residues at the intersection of the A, G, and H helices of the protein, with little other secondary structure and no other tertiary structure. Relaxation kinetics studies were carried out to characterize the dynamics of core melting and formation in this protein. The unfolding and/or refolding response is induced by a laser-induced temperature jump between the folded and unfolded forms of E, and structural changes are monitored using the infrared amide I' absorbance at 1648-1651 cm(-1) that reports on the formation of solvent-protected, native-like helix in the core and by fluorescence emission changes from apomyoglobin's Trp14, a measure of burial of the indole group of this residue. The fluorescence kinetics data are monoexponential with a relaxation time of 14 micros. However, infrared kinetics data are best fit to a biexponential function with relaxation times of 14 and 59 micros. These relaxation times are very fast, close to the limits placed on folding reactions by diffusion. The 14 micros relaxation time is weakly temperature dependent and thus represents a pathway that is energetically downhill. The appearance of this relaxation time in both the fluorescence and infrared measurements indicates that this folding event proceeds by a concomitant formation of compact secondary and tertiary structures. The 59 micros relaxation time is much more strongly temperature dependent and has no fluorescence counterpart, indicating an activated process with a large energy barrier wherein nonspecific hydrophobic interactions between helix A and the G and H helices cause some helix burial but Trp14 remains solvent exposed. These results are best fit by a multiple-pathway kinetic model when U collapses to form the various folded core structures of E. Thus, the results suggest very robust dynamics for core formation involving multiple folding pathways and provide significant insight into the primary processes of protein folding. |
| Address |
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA |
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English |
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Series Issue |
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Edition |
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| ISSN |
0006-2960 |
ISBN |
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| Notes |
PMID:11318635 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3789 |
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| Author |
Hedberg, Y.; Dalin, A.-M.; Ohagen, P.; Holm, K.R.; Kindahl, H. |
| Title |
Effect of oestrous-cycle stage on the response of mares in a novel object test and isolation test |
Type |
Journal Article |
| Year |
2005 |
Publication |
Reproduction in Domestic Animals = Zuchthygiene |
Abbreviated Journal |
Reprod Domest Anim |
| Volume |
40 |
Issue |
5 |
Pages |
480-488 |
| Keywords |
Animals; Behavior, Animal/*physiology; Cross-Over Studies; Diestrus/*physiology; Estrus/*physiology; Female; Heart Rate/*physiology; Horses/*physiology; Questionnaires |
| Abstract |
In various species, sex, hormonal treatments and oestrous-cycle stage have been shown to affect the animal's response in behavioural tests. Few such studies have been performed in the horse. The main aim of the present study was to investigate whether oestrous-cycle stage affects mares' response to a novel object test and isolation test and, in part, to study whether mares, assumed to suffer from oestrous-related behavioural problems, respond differently in these tests when compared with controls. Twelve mares were tested twice, in oestrus and dioestrus, in a crossover design. Seven behavioural and two heart rate variables were measured for the novel object test and two heart rate variables for the isolation test. Oestrous-cycle stage and whether a mare was classified as a 'problem' mare did not affect the mare's response. However, test order, i.e. the cycle stage a mare was tested in first, affected its reaction. This effect could partly be explained by significant differences between test occasions 1 and 2 in three behavioural variables and one heart rate variable (p < 0.05) in the novel object test. The mares explored the novel object more and had a higher mean heart rate in the first test. Exploring the novel object more could largely be attributed to those mares tested in dioestrus first, perhaps indicating that the mares in oestrus were less receptive to the novel object. The reason for the differences between test occasions could be an effect of learning or habituation. |
| Address |
Division of Comparative Reproduction, Obstetrics and Udder Health, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden. ylva.hedberg@kv.slu.se |
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English |
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Edition |
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| ISSN |
0936-6768 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:16149956 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
5170 |
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| Author |
Lees, P.; Tavernor, W.D. |
| Title |
Influence of halothane and catecholamines on heart rate and rhythm in the horse |
Type |
Journal Article |
| Year |
1970 |
Publication |
British journal of pharmacology |
Abbreviated Journal |
Br J Pharmacol |
| Volume |
39 |
Issue |
1 |
Pages |
149-159 |
| Keywords |
Anesthesia, Inhalation; Animals; Arrhythmia/*chemically induced; Atropine/pharmacology; Catecholamines/*pharmacology; Consciousness; Epinephrine/administration & dosage; Ethers; Female; Halothane/*pharmacology; Heart Rate/*drug effects; Horses; Hypercapnia/physiopathology; Isoproterenol/pharmacology; Male; Norepinephrine/pharmacology; Propranolol/pharmacology |
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English |
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Series Issue |
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Edition |
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| ISSN |
0007-1188 |
ISBN |
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Medium |
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Conference |
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| Notes |
PMID:5420092 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
103 |
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| Author |
Mills, D.S. |
| Title |
Comments about the importance of behaviour to equine clinicians |
Type |
Journal Article |
| Year |
2007 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
39 |
Issue |
1 |
Pages |
95 |
| Keywords |
Animals; *Behavior, Animal/physiology; *Clinical Competence; Horses/*physiology; Humans; Veterinarians/*psychology; Veterinary Medicine/*standards |
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English |
Summary Language |
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Series Issue |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:17228604 |
Approved |
no |
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Serial |
1839 |
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| Author |
Eisgruber, H.; Stolle, F.A. |
| Title |
[Clostridia in carcasses and fresh meat--a literature review] |
Type |
Journal Article |
| Year |
1992 |
Publication |
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of Veterinary Medicine. Series B |
Abbreviated Journal |
Zentralbl Veterinarmed B |
| Volume |
39 |
Issue |
10 |
Pages |
746-754 |
| Keywords |
Abattoirs; Animals; Cattle; Clostridium/*growth & development; *Food Microbiology; Horses; Meat/*microbiology; Muscles/*microbiology; Sheep; Swine |
| Abstract |
Clostridia are of large clinical importance as well as in the field of food hygiene, where they are responsible for spoilage but they also have a certain significance as food poisoning organisms. Information on the ecology of Clostridia in samples of deep muscle tissue of slaughtered animals is insufficient. This article is intended to increase the knowledge on the occurrence of different Clostridia species in slaughtered animals. The main emphasis is put on the significance of clostridia in meat hygiene. The theoretical basis of the so called original content of microorganisms (intrinsic bacteria), the factors and pathways of Clostridia spreading in muscles and organs are demonstrated. |
| Address |
Institut fur Hygiene und Technologie, Ludwig-Maximilians-Universitat Munchen |
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Editor |
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| Language |
German |
Summary Language |
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Original Title |
Clostridien in Schlachttierkorpern und frischem Fleisch--Eine Literaturubersicht |
| Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0514-7166 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:1492516 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2668 |
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| Author |
Marinier, S.L.; Alexander, A.J. |
| Title |
The use of a maze in testing learning and memory in horses |
Type |
Journal Article |
| Year |
1994 |
Publication |
Applied Animal Behaviour Science |
Abbreviated Journal |
Appl. Anim. Behav. Sci. |
| Volume |
39 |
Issue |
2 |
Pages |
177-182 |
| Keywords |
Horses; Learning; Memory; Maze; Exercise |
| Abstract |
Two mazes were used to test the learning ability and memory of horses, and changes in these abilities. Testing was done on four occasions. On Occasion 1, the horses were run through Maze A until they had reached the criterion of three consecutive correct runs. A week later (Occasion 2), they were retested in Maze A to the same criterion as a measure of memory. On Occasion 3,2 months later, the horses were run through Mazes A and B until they reached the criterion. Occasion 4 took place 1 week later when they were run through Mazes A and B. An estimation of changes in ability to learn came from a comparison of results from Occasions 1 and 3. Similarly, changes in ability to remember came from a comparison of results from Occasions 2 and 4. Nine horses with a variable amount of riding training were the subjects. All horses were able to learn the maze, but the ability varied among horses. There was no obvious correlation between quality of handling of the horses and learning ability. Once the horses had learned the maze, they remembered it perfectly on subsequent occasions. There were changes in the memory and learning ability of the horses, but no clear explanation for this could be found. |
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Approved |
no |
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Equine Behaviour @ team @ |
Serial |
3573 |
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| Author |
Timney, B.; Keil, K. |
| Title |
Local and global stereopsis in the horse |
Type |
Journal Article |
| Year |
1999 |
Publication |
Vision Research |
Abbreviated Journal |
Vision Res |
| Volume |
39 |
Issue |
10 |
Pages |
1861-1867 |
| Keywords |
Animals; Depth Perception/*physiology; Female; Horses/*physiology; Pattern Recognition, Visual/physiology; Psychophysics; Sensory Thresholds/physiology; Vision, Binocular/physiology; Vision, Monocular/physiology |
| Abstract |
Although horses have laterally-placed eyes, there is substantial binocular overlap, allowing for the possibility that these animals have stereopsis. In the first experiment of the present study we measured local stereopsis by obtaining monocular and binocular depth thresholds for renal depth stimuli. On all measures, the horses' binocular performance was superior to their monocular. When depth thresholds were obtained, binocular thresholds were several times superior to those obtained monocularly, suggesting that the animals could use stereoscopic information when it was available. The binocular thresholds averaged about 15 min arc. In the second experiment we obtained evidence for the presence of global stereopsis by testing the animals' ability to discriminate between random-dot stereograms with and without consistent disparity information. When presented with such stimuli they showed a strong preference for the cyclopean equivalent of the positive stimulus with the real depth. These results provide the first behavioral demonstration of a full range of stereoscopic skills in a lateral-eyed mammal. |
| Address |
Department of Psychology, Faculty of Social Science, University of Western Ontario, London, Canada. timney@julian.uwo.ca |
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English |
Summary Language |
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Series Issue |
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Edition |
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| ISSN |
0042-6989 |
ISBN |
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Medium |
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Conference |
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| Notes |
PMID:10343877 |
Approved |
yes |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3580 |
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| Author |
Kristiansen, K.K.; Kold, S.E. |
| Title |
Multivariable analysis of factors influencing outcome of 2 treatment protocols in 128 cases of horses responding positively to intra-articular analgesia of the distal interphalangeal joint |
Type |
Journal Article |
| Year |
2007 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
39 |
Issue |
2 |
Pages |
150-156 |
| Keywords |
Analgesia/methods/*veterinary; Anesthesia, Local/methods/veterinary; Animals; Anti-Inflammatory Agents/therapeutic use; Female; Foot Diseases/drug therapy/prevention & control/*veterinary; Glycosaminoglycans/therapeutic use; Horse Diseases/*drug therapy/prevention & control; Horses; Injections, Intra-Articular/*veterinary; Joint Diseases/drug therapy/prevention & control/veterinary; Lameness, Animal/drug therapy/prevention & control; Male; Methylprednisolone/analogs & derivatives/therapeutic use; Multivariate Analysis; Pain/prevention & control/veterinary; Retrospective Studies; Time Factors; *Toe Joint/drug effects/pathology; Treatment Outcome |
| Abstract |
REASONS FOR PERFORMING STUDY: There is limited knowledge available of factors influencing response to treatments of the DIP-joint in horses with lameness responding to diagnostic analgesia of the DIP-joint. For this reason a multivariable statistical analysis was performed. HYPOTHESIS: Horses with lameness reduced by > or = 75% 10 min after intra-articular analgesia of the DIP-joint, can be treated successfully by intra-articular medication of the joint. Multiple factors influence the response to treatment. METHODS: The study was performed retrospectively based on clinical records of horses treated with either polysulphated glycosaminoglycan (PSGAG) or methylprednisolone acetate (MPA) in the DIP-joint between January 1996 and January 2003. Information was collected from clinical records and from the owners of the horses via a detailed questionnaire, in which they described their perception of the outcome a minimum of one year after treatment. Allocation of the horses to the 2 treatment groups was done mainly because of a change in treatment policy. In Regime A all horses received 3 intra-articular injections of PSGAG approximately 8 days apart, whereas in Regime B all horses received a single intra-articular injection of MPA as a first treatment. If the horse did not improve sufficiently to return to work by 4 weeks, a series of 3 intra-articular PSGAG injections was administered. RESULTS: Of the horses receiving Regime A, 67% had a successful outcome, compared with 46% of the group receiving Regime B. A significantly better result was obtained in dressage horses than in jumping horses (eventing and showjumping). Other variables such as age, duration of lameness, distribution of lameness, degree of lameness, response to DIP-joint analgesia and radiographic observations were also associated with success of treatment. CONCLUSIONS AND POTENTIAL RELEVANCE: There is a rationale for using either PSGAG or MPA intra-articularly in the treatment of lameness, reduced > or = 75% within 10 min of analgesia of the DIP-joint. |
| Address |
Willesley Equine Clinic Ltd., Tetbury, Gloucestershire, GL8 8QU UK |
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English |
Summary Language |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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Conference |
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| Notes |
PMID:17378444 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3707 |
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| Author |
Verheyen, K.; Price, J.; Lanyon, L.; Wood, J. |
| Title |
Exercise distance and speed affect the risk of fracture in racehorses |
Type |
Journal Article |
| Year |
2006 |
Publication |
Bone |
Abbreviated Journal |
Bone |
| Volume |
39 |
Issue |
6 |
Pages |
1322-1330 |
| Keywords |
Animals; Case-Control Studies; Cohort Studies; England; Exertion; Female; Fractures, Bone/etiology/*veterinary; Horse Diseases/*etiology; Horses/*injuries; Male; Physical Conditioning, Animal/adverse effects/methods; Poisson Distribution; Prospective Studies; Regression Analysis; Risk Factors; Running/injuries/physiology |
| Abstract |
In order to gain insight into those training regimens that can minimise the risk of fracture in athletic populations, we conducted a large epidemiological study in racehorses. Thoroughbred racehorses provide a suitable model for studying fracture development and exercise-related risk factors in physically active populations. They represent a homogeneous population, undertaking intensive exercise programmes that are sufficiently heterogeneous to determine those factors that influence injury risk. Daily exercise information was recorded for a cohort of 1178 thoroughbreds that were monitored for up to 2 years. A total of 148 exercise-induced fractures occurred in the study population. Results from a nested case-control study showed a strong interactive effect of exercise distances at different speeds on fracture risk. Horses that exceeded 44 km at canter (< or =14 m/s) and 6 km at gallop (>14 m/s) in a 30-day period were at particularly increased risk of fracture. These distances equate to ca. 7700 bone loading cycles at canter and 880 loading cycles at gallop. Fifty-six fractures occurred in the subset of study horses that were followed since entering training as yearlings, when skeletally immature (n = 335). Cohort analysis of this data set showed that, in previously untrained bones, accumulation of canter exercise increased the risk of fracture (P < or = 0.01), whereas accumulation of high-speed gallop exercise had a protective effect (P < 0.01). However, increasing distances at canter and gallop in short time periods (up to one month) were associated with an increasing fracture risk. All training exercise involves a balance between the risk of fracture inherent in exposure to loading and the beneficial effect that loading has by stimulating bone cells to produce a more robust architecture. Results from our study provide important epidemiological evidence of the effects of physical exercise on bone adaptation and injury risk and can be used to inform the design of safer exercise regimens in physically active populations. |
| Address |
Epidemiology Unit, Animal Health Trust, Newmarket, United Kingdom. kverheyen@rvc.ac.uk |
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English |
Summary Language |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
8756-3282 |
ISBN |
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Conference |
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| Notes |
PMID:16926125 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4030 |
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| Author |
Houpt, K.A. |
| Title |
Why horse behaviour is important to the equine clinician |
Type |
Journal Article |
| Year |
2006 |
Publication |
Equine veterinary journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
38 |
Issue |
5 |
Pages |
386-387 |
| Keywords |
Accidents, Occupational/prevention & control; Aggression; Animals; *Behavior, Animal/physiology; Clinical Competence; Fear; Horses/*physiology; Humans; Veterinarians/psychology; Veterinary Medicine/*standards |
| Abstract |
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| Address |
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA |
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English |
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Series Issue |
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Edition |
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0425-1644 |
ISBN |
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Conference |
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| Notes |
PMID:16986596 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
30 |
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