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Author |
Muscatello, G.; Anderson, G.A.; Gilkerson, J.R.; Browning, G.F. |
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Title  |
Associations between the ecology of virulent Rhodococcus equi and the epidemiology of R. equi pneumonia on Australian thoroughbred farms |
Type |
Journal Article |
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Year |
2006 |
Publication |
Applied and Environmental Microbiology |
Abbreviated Journal |
Appl Environ Microbiol |
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Volume |
72 |
Issue |
9 |
Pages |
6152-6160 |
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Keywords |
Actinomycetales Infections/epidemiology/microbiology/*veterinary; Air Microbiology; Animal Husbandry; Animals; Animals, Newborn; Australia/epidemiology; Colony Count, Microbial; DNA, Bacterial/genetics; Ecosystem; Horse Diseases/epidemiology/*microbiology; Horses; Pneumonia, Bacterial/epidemiology/microbiology/*veterinary; Rhodococcus equi/genetics/isolation & purification/*pathogenicity; Soil Microbiology; Virulence |
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Abstract |
The ecology of virulent strains of Rhodococcus equi on horse farms is likely to influence the prevalence and severity of R. equi pneumonia in foals. This study examined the association between the ecology of virulent R. equi and the epidemiology of R. equi pneumonia by collecting air and soil samples over two breeding seasons (28 farm-year combinations) on Thoroughbred breeding farms with different reported prevalences of R. equi pneumonia. Colony blotting and DNA hybridization were used to detect and measure concentrations of virulent R. equi. The prevalence of R. equi pneumonia was associated with the airborne burden of virulent R. equi (both the concentration and the proportion of R. equi bacteria that were virulent) but was not associated with the burden of virulent R. equi in the soil. Univariable screening and multivariable model building were used to evaluate the effect of environmental and management factors on virulent R. equi burdens. Lower soil moisture concentrations and lower pasture heights were significantly associated with elevated airborne concentrations of virulent R. equi, as were the holding pens and lanes, which typically were sandy, dry, and devoid of pasture cover. Few variables appeared to influence concentrations of virulent R. equi in soil. Acidic soil conditions may have contributed to an elevated proportion of virulent strains within the R. equi population. Environmental management strategies that aim to reduce the level of exposure of susceptible foals to airborne virulent R. equi are most likely to reduce the impact of R. equi pneumonia on endemically affected farms. |
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School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia. mug@unimelb.edu.au |
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0099-2240 |
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PMID:16957241 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2622 |
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Author |
Milinovich, G.J.; Trott, D.J.; Burrell, P.C.; van Eps, A.W.; Thoefner, M.B.; Blackall, L.L.; Al Jassim, R.A.M.; Morton, J.M.; Pollitt, C.C. |
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Title |
Changes in equine hindgut bacterial populations during oligofructose-induced laminitis |
Type |
Journal Article |
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Year |
2006 |
Publication |
Environmental Microbiology |
Abbreviated Journal |
Environ Microbiol |
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Volume |
8 |
Issue |
5 |
Pages |
885-898 |
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Keywords |
Animal Feed; Animals; Bacteria/classification/*isolation & purification; DNA, Bacterial/analysis; Disease Models, Animal; Feces/microbiology; Foot Diseases/etiology/microbiology/*veterinary; Horse Diseases/*etiology/metabolism/microbiology; Horses; In Situ Hybridization, Fluorescence; Intestines/*microbiology; Oligosaccharides/*administration & dosage/*metabolism; Phylogeny; Polymerase Chain Reaction; RNA, Bacterial/analysis; RNA, Ribosomal, 16S/analysis |
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Abstract |
In the horse, carbohydrate overload is thought to play an integral role in the onset of laminitis by drastically altering the profile of bacterial populations in the hindgut. The objectives of this study were to develop and validate microbial ecology methods to monitor changes in bacterial populations throughout the course of experimentally induced laminitis and to identify the predominant oligofructose-utilizing organisms. Laminitis was induced in five horses by administration of oligofructose. Faecal specimens were collected at 8 h intervals from 72 h before to 72 h after the administration of oligofructose. Hindgut microbiota able to utilize oligofructose were enumerated throughout the course of the experiment using habitat-simulating medium. Isolates were collected and representatives identified by 16S rRNA gene sequencing. The majority of these isolates collected belonged to the genus Streptococcus, 91% of which were identified as being most closely related to Streptococcus infantarius ssp. coli. Furthermore, S. infantarius ssp. coli was the predominant oligofructose-utilizing organism isolated before the onset of lameness. Fluorescence in situ hybridization probes developed to specifically target the isolated Streptococcus spp. demonstrated marked population increases between 8 and 16 h post oligofructose administration. This was followed by a rapid population decline which corresponded with a sharp decline in faecal pH and subsequently lameness at 24-32 h post oligofructose administration. This research suggests that streptococci within the Streptococcus bovis/equinus complex may be involved in the series of events which precede the onset of laminitis in the horse. |
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Address |
Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Queensland, Australia. g.milinovich@uq.edu.au |
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ISSN |
1462-2912 |
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Notes |
PMID:16623745 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2625 |
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Author |
Muscatello, G.; Gilkerson, J.R.; Browning, G.F. |
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Title |
Comparison of two selective media for the recovery, isolation, enumeration and differentiation of Rhodococcus equi |
Type |
Journal Article |
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Year |
2007 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
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Volume |
119 |
Issue |
2-4 |
Pages |
324-329 |
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Abstract |
The use of selective media to facilitate the isolation of Rhodococcus equi from environmental and clinical samples has aided studies of the ecology of R. equi and the epidemiology of disease caused by R. equi. Here, we compared the efficacy of two selective media (NANAT and modified CAZ-NB) for the recovery of six defined strains of R. equi and for the isolation and enumeration of both avirulent and virulent R. equi from 60 paired soil samples from horse farms using colony blotting and DNA hybridisation. No difference was found between the two media in the recoverability of defined strains of R. equi or the proportion of soil cultures positive for R. equi or virulent R. equi. NANAT medium was significantly less inhibitory of bacterial growth from soil culture compared to mCAZ-NB (P = 0.001), but there was no difference between the media in the number of R. equi colonies recovered. Soil cultured on mCAZ-NB medium yielded a significantly greater number of virulent R. equi colonies than NANAT (P = 0.03). The proportion of R. equi that were virulent in soil cultures on mCAZ-NB (32%) was more than three times that seen in cultures on NANAT (9%). Thus modified CAZ-NB appeared to be a better selective media for studies where the optimal recovery of virulent R. equi is required, such as in studies of the gastrointestinal carriage of virulent R. equi and of subclinically infected foals. |
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Equine Infectious Disease Laboratory, School of Veterinary Science, The University of Melbourne, Victoria 3010, Australia. mug@unimelb.edu.au |
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ISSN |
0378-1135 |
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Notes |
PMID:17084043 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2621 |
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Author |
Kulikova, E.E; Isaeva, A.S.; Rotkina, A.S.; Manykin, A.A.; Letarov, A.V. |
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Title |
Diversity and dynamics of bacteriophages in horse feces |
Type |
Journal Article |
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Year |
2007 |
Publication |
Microbiology |
Abbreviated Journal |
Microbiology |
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Volume |
76 |
Issue |
2 |
Pages |
271-278 |
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Abstract |
The complex cellulolytic microbial community of the horse intestines is a convenient model for studying the ecology of bacteriophages in natural habitats. Unlike the rumen of the ruminants, this community of the equine large intestine is not subjected to digestion. The inner conditions of the horse gut are much more stable in comparison to other mammals, due to the fact that the horse diet remains almost unchanged and the intervals between food consumption and defecation are much shorter than the whole digestive cycle. The results of preliminary analysis of the structure and dynamics of the viral community of horse feces, which combines direct and culture methods, are presented. In horse fecal samples, we detected more than 60 morphologically distinct phage types, the majority of which were present as a single phage particle. This indicates that the community includes no less than several hundreds of phage types. Some phage types dominated and constituted 5-11% of the total particle count each. The most numerous phage type had an unusual morphology: the tails of its members were extremely long (about 700 nm), flexible, and irretractable, while their heads were 100 nm in diameter. Several other phage types with similar but not identical properties were detected. The total coliphage plaque count of the samples taken from three animals revealed significant fluctuations in the phage titers. During the observation time, the maximum titer ranged within four orders of magnitude (10(3)-10(7) plaque forming units (PFU)/g); the minimum titer ranged within two orders of magnitude. The samples contained two to five morphologically distinct and potentially competitive coliphage types, specific to a single Escherichia coli strain. |
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Russian |
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Series Volume |
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ISSN |
0026-3656 |
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Notes |
PMID:17583225 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2618 |
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Author |
Takai, S.; Narita, K.; Ando, K.; Tsubaki, S. |
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Title |
Ecology of Rhodococcus (Corynebacterium) equi in soil on a horse-breeding farm |
Type |
Journal Article |
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Year |
1986 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
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Volume |
12 |
Issue |
2 |
Pages |
169-177 |
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Keywords |
Actinomycetales/classification/*growth & development; Animals; Corynebacterium/classification/*growth & development; Feces/microbiology; Female; Horses; Serotyping; *Soil Microbiology |
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Abstract |
The ecology of Rhodococcus (Corynebacterium) equi in soil was studied on a horse-breeding farm. R. equi was cultured from soil at a depth of 0, 10, and 20 cm on the six sites of the farm at monthly intervals for 10 months from March to December of 1983. The highest numbers of R. equi were found in the surface soil. The mean number of bacteria in soil samples at every depth increased remarkably from 0 or 10(2) to 10(4) colony-forming units (CFU) g-1 of soil in the middle of April, and later decreased gradually. R. equi inoculated into six soil exudate broths prepared from surface soils at separate sites yielded suspensions with different optical densities, indicating differences in growth. The distribution of serotypes in the soil was similar to that in the horses on the farm. These findings indicated that R. equi could multiply in the soil and flourish in the cycle existing between horses and their soil environment. |
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English |
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Abbreviated Series Title |
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Series Volume |
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Edition |
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ISSN |
0378-1135 |
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Notes |
PMID:3750818 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2683 |
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Author |
Barton, M.D.; Hughes, K.L. |
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Title |
Ecology of Rhodococcus equi |
Type |
Journal Article |
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Year |
1984 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
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Volume |
9 |
Issue |
1 |
Pages |
65-76 |
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Keywords |
Actinomycetales/growth & development/immunology/*isolation & purification; Animal Husbandry; Animals; Antigens, Bacterial/immunology; Artiodactyla/*microbiology; Australia; Digestive System/microbiology; Ecology; Feces/*microbiology; Horses/*microbiology; Hypersensitivity, Delayed; Rabbits/microbiology; *Soil Microbiology |
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Abstract |
A selective broth enrichment technique was used to study the distribution of Rhodococcus equi in soil and grazing animals. Rhodococcus equi was isolated from 54% of soils examined and from the gut contents, rectal faeces and dung of all grazing herbivorous species examined. Rhodococcus equi was not isolated from the faeces or dung of penned animals which did not have access to grazing. The isolation rate from dung was much higher than from other samples and this was found to be due to the ability of R. equi to multiply more readily in dung. Delayed hypersensitivity tests were carried out on horses, sheep and cattle, but only horses reacted significantly. The physiological characteristics of R. equi and the nature of its distribution in the environment suggested that R. equi is a soil organism. |
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0378-1135 |
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Notes |
PMID:6719819 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2688 |
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Author |
Takai, S.; Fujimori, T.; Katsuzaki, K.; Tsubaki, S. |
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Title |
Ecology of Rhodococcus equi in horses and their environment on horse-breeding farms |
Type |
Journal Article |
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Year |
1987 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
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Volume |
14 |
Issue |
3 |
Pages |
233-239 |
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Keywords |
Actinomycetales Infections/*veterinary; Animals; Animals, Newborn/*microbiology; *Environmental Microbiology; Feces/microbiology; Female; Horse Diseases/*microbiology; Horses/*microbiology; Rhodococcus/*isolation & purification |
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Abstract |
Quantitative culture of R. equi in the feces of dams and foals, in the air of the stalls and in the soil of the paddocks was carried out on three horse-breeding farms during the foaling season. The isolation rates of R. equi from the feces of dams from the 3 farms suddenly increased to approximately 80% at the end of March, when the snow in the paddocks finished melting, and remained at that level during April and May. The mean number of R. equi and the isolation rate of R. equi from the feces of dams on the farms were investigated for 5 weeks before and 5 weeks after delivery. During the 10 weeks, there were no differences in the isolation rate or in the mean number of R. equi from the feces of dams. R. equi was first isolated from the feces of the foals born in February and the middle of March at 3-4 weeks of age, on the other hand, it was first isolated from the feces of foals born in the end of March and April at 1-2 weeks of age. The number of R. equi in the soil collected from the paddocks used by dams during the winter was approximately 10(2)-10(4) g-1 of soil during the experiment. R. equi was isolated from the air in the stalls at the end of March and the number of R. equi in the air increased particularly on dry and windy days.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Address |
Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Aomori, Japan |
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0378-1135 |
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Notes |
PMID:3672865 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2679 |
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Author |
Tumova, B. |
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Title |
Equine influenza--a segment in influenza virus ecology |
Type |
Journal Article |
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Year |
1980 |
Publication |
Comparative Immunology, Microbiology and Infectious Diseases |
Abbreviated Journal |
Comp Immunol Microbiol Infect Dis |
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Volume |
3 |
Issue |
1-2 |
Pages |
45-59 |
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Keywords |
Animals; Antigens, Viral; Genes, Viral; Horse Diseases/*microbiology; Horses; Influenza A virus/immunology/pathogenicity/*physiology; Orthomyxoviridae Infections/microbiology/*veterinary; Viral Proteins/analysis |
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English |
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Abbreviated Series Title |
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ISSN |
0147-9571 |
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Notes |
PMID:6258849 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2691 |
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Author |
Wang, L.Y. |
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Title |
Host preference of mosquito vectors of Japanese encephalitis |
Type |
Journal Article |
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Year |
1975 |
Publication |
Zhonghua Minguo wei Sheng wu xue za zhi = Chinese Journal of Microbiology |
Abbreviated Journal |
Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi |
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Volume |
8 |
Issue |
4 |
Pages |
274-279 |
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Keywords |
Animals; *Behavior, Animal; Birds/blood; *Culex; Ecology; Encephalitis, Japanese/*transmission; *Feeding Behavior; Female; Humans; *Insect Vectors; Mammals/blood; Species Specificity; Taiwan |
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Abstract |
The host preference of 4 Culex mosquito species collected in Miaoli and Pingtung counties, Taiwan was studied by capillary precipitin method. Antisera to alum-precipitated sera of man, bovine, swine, rabbit, horse, dog, cat, mouse, chicken, duck, and pigeon were produced in rabbits and reacted with 758 mosquito blood meals among which reactions to one or more antisera. Culex annulus and Culex tritaeniorhynchus summorosus showed a great avidity for pig, and Culex fuscocephala for bovine. Culex pipiens fatigans was ornithophilic. None of 110 C. t. summorosus and 2.4% of 223 C. annulus had fed on man. Among 66 samples of C.p. fatigans tested 10.3% had fed on man, while none of 359 C. fuscocephala did. It seems that the latter does not act as a primary vector of Japanese encephalitis. |
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0009-4587 |
ISBN |
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Notes |
PMID:181218 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2702 |
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Author |
Endy, T.P.; Nisalak, A. |
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Title |
Japanese encephalitis virus: ecology and epidemiology |
Type |
Journal Article |
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Year |
2002 |
Publication |
Current Topics in Microbiology and Immunology |
Abbreviated Journal |
Curr Top Microbiol Immunol |
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Volume |
267 |
Issue |
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Pages |
11-48 |
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Keywords |
Animals; Birds/virology; Climate; Culicidae/virology; Disease Outbreaks/history; Ecosystem; Encephalitis Virus, Japanese/*pathogenicity; Encephalitis, Japanese/*epidemiology/*etiology/history/transmission; History, 20th Century; Horses/virology; Humans; Insect Vectors; Japan/epidemiology; Risk Factors; Swine/virology; Thailand/epidemiology; Viral Vaccines/pharmacology |
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Address |
Department of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences (USAMC-AFRIMS), 315/6 Rajvithi Road, Bangkok 10400, Thailand |
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English |
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0070-217X |
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Notes |
PMID:12082986 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2643 |
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