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Young, T.; Creighton, E.; Smith, T.; Hosie, C. |
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Title |
A novel scale of behavioural indicators of stress for use with domestic horses |
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Journal Article |
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Year |
2012 |
Publication |
Applied Animal Behaviour Science |
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140 |
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1–2 |
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33-43 |
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Horse; Behaviour scores; Cortisol; Saliva; Welfare assessment; Non-invasive |
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Abstract |
Behaviour scores (BS) offer non-invasive, objective and easy to use ways of assessing welfare in animals. Their development has, however, largely focused on behavioural reactions to stressful events (often induced), and little use of physiological measures has been made to underpin and validate the behavioural measures. This study aimed to develop a physiologically validated scale of behavioural indicators of stress for the purpose of welfare assessment in stabled domestic horses. To achieve this, behavioural and physiological data were collected from 32 horses that underwent routine husbandry procedures. Principal component analysis (PCA) of the behavioural and physiological data revealed three meaningful components that were used as the basis of the scale. Analysis of video clips of the horses’ responses to the husbandry procedures was undertaken by a panel of equestrian industry professionals using a free choice profiling (FCP) methodology. These results were added to the scale along with key definitions from relevant literature. Salivary cortisol levels were significantly correlated with the BS confirming the scale was meaningful and reflected physiological stress. The scale offers an easy to use ‘tool’ for rapid, reliable non-invasive welfare assessment in horses, and reduces the need for potentially invasive physiological measures. |
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0168-1591 |
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Equine Behaviour @ team @ |
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5637 |
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Author |
Skandakumar, S.; Stodulski, G.; Hau, J. |
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Title |
Salivary IgA: a Possible Stress Marker In Dogs |
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Abstract |
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Year |
1995 |
Publication |
Animal Welfare |
Abbreviated Journal |
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Volume |
4 |
Issue |
4 |
Pages |
339-350 |
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Animal Welfare; Behaviour; Cortisol; Dog; Salivary Iga (S-Iga); Stress; Well-Being |
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Abstract |
Stress in humans has been reported to be associated with a decrease in the salivary immunoglobulin A (s-IgA) levels enabling the possible use of s-IgA to assess stress. Prolonged stress, if reliably assessed in a non-invasive manner, may be used to assess animal welfare. This study analysed groups of dogs undergoing physical and temperamental training and s-IgA levels were measured by rocket immunoelectrophoresis in prospective samples. Behavioural assessment was carried out and cortisol levels in saliva were measured by ELISA. A significant negative correlation (P < 0.007) between the logarithmic cortisol concentrations and s-IgA levels in saliva was recorded. The behavioural assessment of the dogs agreed well with the biochemical markers. It is concluded that IgA levels in saliva may be a useful marker of dog well-being and that stress results in decreased s-IgA levels. |
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Equine Behaviour @ team @ |
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5964 |
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Vetvik, H.; Grewal, H.M.S.; Haugen, I.L.; Åhrén, C.; Haneberg, B. |
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Title |
Mucosal antibodies can be measured in air-dried samples of saliva and feces |
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Journal Article |
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Year |
1998 |
Publication |
Journal of Immunological Methods |
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215 |
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1–2 |
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163-172 |
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Saliva; Feces; IgA; IgG; Air-drying |
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IgA antibodies reflecting airways or intestinal mucosal immune responses can be found in saliva and feces, respectively, and IgG antibodies reflecting serum antibodies can be found in saliva. In this study, antibodies were detected in samples of saliva and feces which had been air-dried at room temperature (+20°C) or +37°C, and stored at these temperatures for up to 6 months. In saliva the antibody levels increased, while the antibodies in feces decreased upon storage. The individual IgA antibody concentrations which were adjusted by using the ratios of specific IgA/total IgA were relatively stable in both saliva and feces, and correlated with corresponding antibody levels in samples which had been stored at -20°C. The results indicate that air-dried saliva and feces can be used for semiquantitative measurements of mucosal antibodies, even after prolonged storage at high temperatures and lack of refrigeration. |
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0022-1759 |
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Equine Behaviour @ team @ |
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5996 |
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Author |
Jafarzadeh A.; Sadeghi M.; Karam G.A.; Vazirinejad R. |
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Title |
Salivary IgA and IgE levels in healthy subjects: relation to age and gender |
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Journal Article |
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Year |
2010 |
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Braz. oral res. |
Abbreviated Journal |
Braz. Oral Res. |
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24 |
Issue |
1 |
Pages |
21-27 |
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Saliva; Immunoglobulin A; Immunoglobulin E; Adult; Child |
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It has been reported that the immune system undergoes age and gender changes. The aim of this study was to investigate the age- and gender-dependent changes of salivary IgA and IgE levels among healthy subjects. A total of 203 healthy individuals (aged 1-70 years) were enrolled in the study. Two milliliters of saliva were collected from all participants, and salivary IgA and IgE levels were measured by the ELISA technique. Mean salivary IgA levels were significantly higher in subjects aged 11-20 years as compared to subjects aged 1-10 years (P < 0.01). Mean salivary IgA levels increased with age up to the age of 60 years, and then slightly decreased in subjects aged 61-70 years. The frequency of subjects with detectable levels of salivary IgE and mean salivary IgE levels gradually increased with age, with maximum levels being observed in the 31-40 years age group and not changing significantly thereafter. The mean levels of salivary IgA and IgE in adults were significantly higher than those observed in children (P < 0.00001 and P < 0.05, respectively). No significant differences were observed between men and women regarding both salivary immunoglobulins. These results showed age-dependent changes of the salivary IgA and IgE levels. Gender had no effect on the salivary levels of IgA and IgE. |
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Equine Behaviour @ team @ |
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6126 |
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Author |
Palm, A.-K.E.; Wattle, O.; Lundström, T.; Wattrang, E. |
![goto web page (via DOI) doi](img/doi.gif)
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Title |
Secretory immunoglobulin A and immunoglobulin G in horse saliva |
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Journal Article |
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Year |
2016 |
Publication |
Veterinary Immunology and Immunopathology |
Abbreviated Journal |
Vet. Immunol. Immunolpathol. |
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180 |
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59-65 |
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Keywords |
Equine; Secretory IgA; IgG; Saliva; Mucosal immunity |
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This study aimed to increase the knowledge on salivary antibodies in the horse since these constitute an important part of the immune defence of the oral cavity. For that purpose assays to detect horse immunoglobulin A (IgA) including secretory IgA (SIgA) were set up and the molecular weights of different components of the horse IgA system were estimated. Moreover, samples from 51 clinically healthy horses were tested for total SIgA and IgG amounts in saliva and relative IgG3/5 (IgG(T)) and IgG4/7 (IgGb) content were tested in serum and saliva. Results showed a mean concentration of 74μg SIgA/ml horse saliva and that there was a large inter-individual variation in salivary SIgA concentration. For total IgG the mean concentration was approx. 5 times lower than that of SIgA, i.e. 20μg IgG/ml saliva and the inter-individual variation was lower than that observed for SIgA. The saliva-serum ratio for IgG isotypes IgG3/5 and IgG4/7 was also assessed in the sampled horses and this analysis showed that the saliva-serum ratio of IgG4/7 was in general approximately 4 times higher than that of IgG3/5. The large inter-individual variation in salivary SIgA levels observed for the normal healthy horses in the present study emphasises the need for a large number of observations when studying this parameter especially in a clinical setting. Moreover, our results also indicated that some of the salivary IgG does not originate from serum but may be produced locally. Thus, these results provide novel insight, and a base for further research, into salivary antibody responses of horses. |
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0165-2427 |
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Equine Behaviour @ team @ |
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6514 |
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Author |
Elsaesser, F.; Klobasa, F.; Ellendorff, F. |
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Title |
ACTH stimulation test for the determination of salivary cortisol and of cortisol responses as markers of the training status/fitness of warm-blooded sports horses] |
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Journal Article |
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Year |
2001 |
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DTW. Deutsche Tierarztliche Wochenschrift |
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Dtsch Tierarztl Wochenschr |
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108 |
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1 |
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31-36 |
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Adrenocorticotropic Hormone/*diagnostic use; Animals; Health; Horses/*physiology; Hydrocortisone/*analysis/*secretion; Male; Orchiectomy; *Physical Conditioning, Animal; Running; Saliva/*chemistry; Walking |
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Previous work (Marc et al., 2000) suggested that plasma cortisol responses to treadmill exercise or ACTH injection are a reliable marker for performance evaluation in warmblood horses. For practical purposes blood sample collections and treadmill exercise tests are somewhat troublesome and time consuming. The goal of this study was thus to evaluate the use of saliva for cortisol determination (by direct EIA) as a marker for performance and to investigate the reliability and repeatability of plasma cortisol responses to a single i.v. injection of ACTH (50 micrograms or 250 micrograms). Furthermore, the effect of training horses for 8 weeks 3 times per week covering the same distance (increasing from 3.5 km during the first week to 8 km during the last week) either by trotting (approximately 240 m/min) or by cantering (375 m/min) was investigated. For this purpose initially ten four-year-old Hannovarian geldings, all reared in the same State stud, were used. Mean overall correlation between salivary cortisol and plasma cortisol concentrations was 0.64 when samples of various points of time were used. However, in spite of attempts to standardize saliva sample collection, correlation between salivary cortisol levels and plasma cortisol levels at distinct points of time in different tests were low and significant (r = 0.85, p < 0.02) only in one test. Thus, salivary cortisol measurements for diagnostic purposes are not reliable or useful. The repeatability of plasma cortisol responses to ACTH for untrained and trained horses were r = 0.86 and r = 0.8 respectively (p < or = 0.01 and p < or = 0.05 respectively). Training horses either by trotting or cantering did not affect the cortisol response either to treadmill exercise or to stimulation by ACTH. It is concluded that the relationship between salivary cortisol levels and plasma cortisol levels is not close enough to allow the use of salivary cortisol determination as marker of the training status/fitness of horses. The repeatability of the cortisol response to ACTH is similar to the cortisol response to treadmill exercise. Based on plasma cortisol responses to ACTH or treadmill exercise training horses by cantering at low speed is not superior to training by trotting for the fitness of horses. |
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Institut fur Tierzucht und Tierverhalten Mariensee (FAL), Holtystrasse 10, 31535 Neustadt. elsaesser@tzv.fal.de |
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German |
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ACTH Stimulationstest und Bestimmung von Cortisol im Blut und Speichel zur Bewertung des Trainingszustands/der Kondition beim Warmblutpferd |
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0341-6593 |
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PMID:11232423 |
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Call Number |
Equine Behaviour @ team @ |
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4053 |
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