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Author Zhao, C.J.; Qin, Y.H.; Lee, X.H.; Wu, C.
Title Molecular and cytogenetic paternity testing of a male offspring of a hinny Type Journal Article
Year 2006 Publication Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie Abbreviated Journal J Anim Breed Genet
Volume 123 Issue 6 Pages 403-405
Keywords Animals; Cytogenetic Analysis; DNA, Mitochondrial/genetics; Equidae/*genetics; Female; Horses/genetics; Hybridization, Genetic; Male; Microsatellite Repeats; Pedigree; Protamines/genetics; Sexual Behavior, Animal
Abstract An alleged male foal of a female mule, whose sire and grandparents were unknown, was identified for its pedigree. Parentage testing was conducted by comparing polymorphism of 12 microsatellite DNA sites and mitochondrial D-loop sequences of the male foal and the female mule. Both the sequence analysis of species-specific DNA fragments and a cytogenetic analysis were performed to identify the species of the foal and its parents. The results showed that the alleged female mule is actually a hinny, and the male foal, which possesses 62 chromosomes, qualifies as an offspring of the female hinny and a jack donkey.
Address Equine Center, China Agricultural University, Beijing, China
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0931-2668 ISBN Medium
Area Expedition Conference
Notes (up) PMID:17177697 Approved no
Call Number Serial 1846
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Author Ishida, N.; Oyunsuren, T.; Mashima, S.; Mukoyama, H.; Saitou, N.
Title Mitochondrial DNA sequences of various species of the genus Equus with special reference to the phylogenetic relationship between Przewalskii's wild horse and domestic horse Type Journal Article
Year 1995 Publication Journal of Molecular Evolution Abbreviated Journal J Mol Evol
Volume 41 Issue 2 Pages 180-188
Keywords Animals; Base Sequence; Chromosomes; Conserved Sequence/genetics; DNA, Mitochondrial/*genetics; Evolution; Genetic Variation/*genetics; Horses/*genetics; Molecular Sequence Data; *Phylogeny; RNA, Transfer, Pro/genetics; Sequence Alignment; Sequence Analysis, DNA
Abstract The noncoding region between tRNAPro and the large conserved sequence block is the most variable region in the mammalian mitochondrial DNA D-loop region. This variable region (ca. 270 bp) of four species of Equus, including Mongolian and Japanese native domestic horses as well as Przewalskii's (or Mongolian) wild horse, were sequenced. These data were compared with our recently published Thoroughbred horse mitochondrial DNA sequences. The evolutionary rate of this region among the four species of Equus was estimated to be 2-4 x 10(-8) per site per year. Phylogenetic trees of Equus species demonstrate that Przewalskii's wild horse is within the genetic variation among the domestic horse. This suggests that the chromosome number change (probably increase) of the Przewalskii's wild horse occurred rather recently.
Address Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0022-2844 ISBN Medium
Area Expedition Conference
Notes (up) PMID:7666447 Approved no
Call Number Equine Behaviour @ team @ Serial 5042
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Author Breen, M.; Downs, P.; Irvin, Z.; Bell, K.
Title Intrageneric amplification of horse microsatellite markers with emphasis on the Przewalski's horse (E. przewalskii) Type Journal Article
Year 1994 Publication Animal Genetics Abbreviated Journal Anim Genet
Volume 25 Issue 6 Pages 401-405
Keywords Animals; DNA, Satellite/*genetics; *Gene Amplification; Gene Frequency; *Genetic Markers; Heterozygote; Horses/*genetics; Species Specificity
Abstract Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was calculated to be 0.9994. The results suggest that it should be possible to use E. caballus-derived microsatellite markers to provide parentage verification and additional valuable information to the captive management of E. przewalskii. The ability to amplify corresponding loci in the remaining five species of the genus was also confirmed, illustrating the general application of markers isolated from the domestic horse to the evaluation of polymorphism in the other six species of the genus.
Address Australian Equine Blood Typing Research Laboratory, University of Queensland, St Lucia
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0268-9146 ISBN Medium
Area Expedition Conference
Notes (up) PMID:7695120 Approved no
Call Number Equine Behaviour @ team @ Serial 2246
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Author Ishida, N.; Hirano, T.; Mukoyama, H.
Title Detection of aberrant alleles in the D-loop region of equine mitochondrial DNA by single-strand conformation polymorphism (SSCP) analysis Type Journal Article
Year 1994 Publication Animal Genetics Abbreviated Journal Anim Genet
Volume 25 Issue 4 Pages 287
Keywords *Alleles; Animals; Base Sequence; *DNA, Mitochondrial; DNA, Single-Stranded/genetics; Female; Gene Frequency; Genomic Imprinting; Horses/*genetics; Male; Molecular Sequence Data; Pedigree; *Polymorphism, Genetic
Abstract
Address Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0268-9146 ISBN Medium
Area Expedition Conference
Notes (up) PMID:7985852 Approved no
Call Number Equine Behaviour @ team @ Serial 2213
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Author Cilnis, M.J.; Kang, W.; Weaver, S.C.
Title Genetic conservation of Highlands J viruses Type Journal Article
Year 1996 Publication Virology Abbreviated Journal Virology
Volume 218 Issue 2 Pages 343-351
Keywords Alphavirus/*genetics; Alphavirus Infections/transmission/veterinary/virology; Amino Acid Sequence; Animals; Base Sequence; Conserved Sequence; Disease Outbreaks; Encephalitis, Viral/veterinary/virology; *Evolution, Molecular; Horses; Molecular Sequence Data; Phylogeny; RNA, Viral/genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Turkeys; Variation (Genetics)/*genetics
Abstract We studied molecular evolution of the mosquito-borne alphavirus Highlands J (HJ) virus by sequencing PCR products generated from 19 strains isolated between 1952 and 1994. Sequences of 1200 nucleotides including portions of the E1 gene and the 3' untranslated region revealed a relatively slow evolutionary rate estimated at 0.9-1.6 x 10(-4) substitutions per nucleotide per year. Phylogenetic trees indicated that all HJ viruses descended from a common ancestor and suggested the presence of one dominant lineage in North America. However, two or more minor lineages probably circulated simultaneously for periods of years to a few decades. Strains isolated from a horse suffering encephalitis, and implicated in a recent turkey outbreak, were not phylogenetically distinct from strains isolated in other locations during the same time periods. Our findings are remarkably similar to those we obtained previously for another North American alphavirus, eastern equine encephalomyelitis virus, with which Highlands J shares primary mosquito and avian hosts, geographical distribution, and ecology. These results support the hypotheses that the duration of the transmission season affects arboviral evolutionary rates and vertebrate host mobility influences genetic diversity.
Address Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0042-6822 ISBN Medium
Area Expedition Conference
Notes (up) PMID:8610461 Approved no
Call Number Equine Behaviour @ team @ Serial 2657
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Author Birch, H.L.; Bailey, A.J.; Goodship, A.E.
Title Macroscopic 'degeneration' of equine superficial digital flexor tendon is accompanied by a change in extracellular matrix composition Type Journal Article
Year 1998 Publication Equine Veterinary Journal Abbreviated Journal Equine Vet J
Volume 30 Issue 6 Pages 534-539
Keywords Animals; Collagen/analysis; DNA/analysis; Extracellular Matrix/*chemistry; Glycosaminoglycans/analysis; Horses/injuries/*physiology; Immunohistochemistry; Rupture/veterinary; Tendon Injuries/metabolism/pathology/veterinary; Tendons/chemistry/*pathology; Water/analysis
Abstract Injuries to the superficial digital flexor tendon are common in horses required to gallop and jump at speed. Partial rupture of this tendon usually occurs in the central core of the midmetacarpal region and may be preceded by localised degenerative changes. Post mortem examination of apparently normal equine flexor tendons has revealed an abnormal macroscopic appearance in the central core, characterised by a reddish discolouration. We have previously shown that there is also physical damage to the collagen fibres. In the present study we tested the hypothesis that the abnormal appearance is accompanied by changes in the composition of the extracellular matrix of the tendon. Biochemical analysis of the extracellular matrix demonstrated an increase in total sulphated glycosaminoglycan content, increase in the proportion of type III collagen and decrease in collagen linked fluorescence in the central core of 'degenerated' tendons relative to tissue from the peripheral region of the same tendon. Dry matter content and total collagen content were not significantly different between tendon zones or normal and 'degenerated' tendons. These changes suggest a change in cell metabolism and matrix turnover in the central core of the tendon and are likely to contribute to a decrease in mechanical properties in this part of the tendon, predisposing to the characteristic partial rupture of the tendon.
Address Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hatfield, UK
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0425-1644 ISBN Medium
Area Expedition Conference
Notes (up) PMID:9844973 Approved no
Call Number Equine Behaviour @ team @ Serial 3794
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Author Oakenfull, E.A.; Ryder, O.A.
Title Mitochondrial control region and 12S rRNA variation in Przewalski's horse (Equus przewalskii) Type Journal Article
Year 1998 Publication Animal Genetics Abbreviated Journal Anim Genet
Volume 29 Issue 6 Pages 456-459
Keywords Animals; DNA, Mitochondrial/*genetics; Female; *Genetic Variation; Horses/*genetics; Male; Pedigree; RNA, Ribosomal/*genetics
Abstract Variation in the control region and the 12S rRNA gene of all surviving mitochondrial lineages of Przewalski's horse was investigated. Variation is low despite the present day population being descended from 13 individuals probably representing animals from three different regions of its range. Phylogenetic comparison of these sequences, with sequences for the domestic horse, does not resolve the ancestral status of either horse.
Address Center for Reproduction of Endangered Species, Zoological Society of San Diego, CA 92112, USA
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0268-9146 ISBN Medium
Area Expedition Conference
Notes (up) PMID:9883508 Approved no
Call Number Equine Behaviour @ team @ Serial 5040
Permanent link to this record