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Author Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B.
Title Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces Type Journal Article
Year 2004 Publication Parasitology Abbreviated Journal Parasitology
Volume 129 Issue (down) Pt 6 Pages 733-739
Keywords Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics
Abstract Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae.
Address Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1820 ISBN Medium
Area Expedition Conference
Notes PMID:15648696 Approved no
Call Number Equine Behaviour @ team @ Serial 2631
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Author Stock, K.F.; Distl, O.
Title Evaluation of expected response to selection for orthopedic health and performance traits in Hanoverian Warmblood horses Type Journal Article
Year 2005 Publication American Journal of Veterinary Research Abbreviated Journal Am J Vet Res
Volume 66 Issue (down) 8 Pages 1371-1379
Keywords Animals; Bone Diseases/genetics/*veterinary; *Breeding; Genetic Predisposition to Disease; Horse Diseases/*genetics; Horses/*genetics; Joint Diseases/genetics/*veterinary; Selection (Genetics)
Abstract OBJECTIVE: To determine whether selection schemes accounting for orthopedic health traits were compatible with breeding progress in performance parameters in Hanoverian Warmblood horses. ANIMALS: 5,928 horses. PROCEDURE: Relative breeding values (RBVs) were predicted for osseous fragments in fetlock (metacarpo- and metatarsophalangeal) and tarsal joints, deforming arthropathy in tarsal joints, and pathologic changes in distal sesamoid bones. Selection schemes were developed on the basis of total indices for radiographic findings (TIR), dressage (TID), and jumping (TIJ). Response to selection was traced over 2 generations of horses for dressage and jumping ability and all-purpose breeding. Development of mean RBVs and mean total indices in sires and prevalences of orthopedic health traits in their offspring were used to assess response to selection. RESULTS: Giving equal weight toTIR andTID, TIJ, or a combined index of 60% TID and 40% TIJ, 43% to 53% of paternal grandsires and 70% to 82% of descending sires passed selection. In each case, RBVs and total indices increased by as much as 9% in selected sires, when compared with all sires, and prevalences of orthopedic health traits in offspring of selected sires decreased relatively by as much as 16%. When selection was exclusively based on TID, TIJ, or TID and TIJ, percentages of selected sires were 44% to 66% in the first and 73% to 84% in the second generation and TID and TIJ increased by 9% to 10% and 19% to 23%, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Compared with exclusively performance-based selection, percentages of selected sires changed slightly and breeding progress in TID, TIJ, or TID and TIJ was only slightly decreased; however, prevalences of orthopedic health traits decreased in offspring of TIR-selected sires.
Address Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover (Foundation), Bunteweg 17p, 30559 Hannover, Germany
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0002-9645 ISBN Medium
Area Expedition Conference
Notes PMID:16173480 Approved no
Call Number Equine Behaviour @ team @ Serial 3713
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Author Sebastiani, F.; Meiswinkel, R.; Gomulski, L.M.; Guglielmino, C.R.; Mellor, P.S.; Malacrida, A.R.; Gasperi, G.
Title Molecular differentiation of the Old World Culicoides imicola species complex (Diptera, Ceratopogonidae), inferred using random amplified polymorphic DNA markers Type Journal Article
Year 2001 Publication Molecular Ecology Abbreviated Journal Mol Ecol
Volume 10 Issue (down) 7 Pages 1773-1786
Keywords Africa; Animals; Ceratopogonidae/*classification/*genetics; Ecology; Evolution, Molecular; Female; *Genetic Markers; Madagascar; Phylogeny; *Polymorphism, Genetic; *Random Amplified Polymorphic DNA Technique; Variation (Genetics)
Abstract Samples of seven of the 10 morphological species of midges of the Culicoides imicola complex were considered. The importance of this species complex is connected to its vectorial capacity for African horse sickness virus (AHSV) and bluetongue virus (BTV). Consequently, the risk of transmission may vary dramatically, depending upon the particular cryptic species present in a given area. The species complex is confined to the Old World and our samples were collected in Southern Africa, Madagascar and the Ivory Coast. Genomic DNA of 350 randomly sampled individual midges from 19 populations was amplified using four 20-mer primers by the random amplified polymorphic DNA (RAPD) technique. One hundred and ninety-six interpretable polymorphic bands were obtained. Species-specific RAPD profiles were defined and for five species diagnostic RAPD fragments were identified. A high degree of polymorphism was detected in the species complex, most of which was observed within populations (from 64 to 76%). Principal coordinate analysis (PCO) and cluster analysis provided an estimate of the degree of variation between and within populations and species. There was substantial concordance between the taxonomies derived from morphological and molecular data. The amount and the different distributions of genetic (RAPD) variation among the taxa can be associated to their life histories, i.e. the abundance and distribution of the larval breeding sites and their seasonality.
Address Department of Animal Biology, Laboratory of Zoology, University of Pavia, Piazza Botta 9, I-27100 Pavia, Italy
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0962-1083 ISBN Medium
Area Expedition Conference
Notes PMID:11472544 Approved no
Call Number Equine Behaviour @ team @ Serial 2647
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Author Novacek, M.J.
Title Mammalian phylogeny: shaking the tree Type Journal Article
Year 1992 Publication Nature Abbreviated Journal Nature
Volume 356 Issue (down) 6365 Pages 121-125
Keywords Animals; Evolution; Fossils; Mammals/classification/*genetics; *Phylogeny
Abstract Recent palaeontological discoveries and the correspondence between molecular and morphological results provide fresh insight on the deep structure of mammalian phylogeny. This new wave of research, however, has yet to resolve some important issues.
Address American Museum of Natural History, New York 10024
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0028-0836 ISBN Medium
Area Expedition Conference
Notes PMID:1545862 Approved no
Call Number Equine Behaviour @ team @ Serial 3546
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Author de Waal, F.B.
Title The end of nature versus nurture Type Journal Article
Year 1999 Publication Scientific American Abbreviated Journal Sci Am
Volume 281 Issue (down) 6 Pages 94-99
Keywords Animals; *Behavior; Behavior, Animal; Ecology; *Environment; Ethology; Evolution; Female; *Genetics; Humans; Instinct; Learning; Male; Sex Characteristics; Twin Studies
Abstract
Address Living Links Center, Yerkes Regional Primate Research Center, Atlanta, USA
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0036-8733 ISBN Medium
Area Expedition Conference
Notes PMID:10614071 Approved no
Call Number refbase @ user @ Serial 192
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Author Zhao, C.J.; Qin, Y.H.; Lee, X.H.; Wu, C.
Title Molecular and cytogenetic paternity testing of a male offspring of a hinny Type Journal Article
Year 2006 Publication Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie Abbreviated Journal J Anim Breed Genet
Volume 123 Issue (down) 6 Pages 403-405
Keywords Animals; Cytogenetic Analysis; DNA, Mitochondrial/genetics; Equidae/*genetics; Female; Horses/genetics; Hybridization, Genetic; Male; Microsatellite Repeats; Pedigree; Protamines/genetics; Sexual Behavior, Animal
Abstract An alleged male foal of a female mule, whose sire and grandparents were unknown, was identified for its pedigree. Parentage testing was conducted by comparing polymorphism of 12 microsatellite DNA sites and mitochondrial D-loop sequences of the male foal and the female mule. Both the sequence analysis of species-specific DNA fragments and a cytogenetic analysis were performed to identify the species of the foal and its parents. The results showed that the alleged female mule is actually a hinny, and the male foal, which possesses 62 chromosomes, qualifies as an offspring of the female hinny and a jack donkey.
Address Equine Center, China Agricultural University, Beijing, China
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0931-2668 ISBN Medium
Area Expedition Conference
Notes PMID:17177697 Approved no
Call Number Serial 1846
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Author Breen, M.; Downs, P.; Irvin, Z.; Bell, K.
Title Intrageneric amplification of horse microsatellite markers with emphasis on the Przewalski's horse (E. przewalskii) Type Journal Article
Year 1994 Publication Animal Genetics Abbreviated Journal Anim Genet
Volume 25 Issue (down) 6 Pages 401-405
Keywords Animals; DNA, Satellite/*genetics; *Gene Amplification; Gene Frequency; *Genetic Markers; Heterozygote; Horses/*genetics; Species Specificity
Abstract Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was calculated to be 0.9994. The results suggest that it should be possible to use E. caballus-derived microsatellite markers to provide parentage verification and additional valuable information to the captive management of E. przewalskii. The ability to amplify corresponding loci in the remaining five species of the genus was also confirmed, illustrating the general application of markers isolated from the domestic horse to the evaluation of polymorphism in the other six species of the genus.
Address Australian Equine Blood Typing Research Laboratory, University of Queensland, St Lucia
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0268-9146 ISBN Medium
Area Expedition Conference
Notes PMID:7695120 Approved no
Call Number Equine Behaviour @ team @ Serial 2246
Permanent link to this record
 

 
Author Nettle, D.
Title The evolution of personality variation in humans and other animals Type Journal Article
Year 2006 Publication The American Psychologist Abbreviated Journal Am Psychol
Volume 61 Issue (down) 6 Pages 622-631
Keywords Animals; Birds; *Evolution; Female; Fishes; Humans; Insects; Male; Personality/*genetics/*physiology
Abstract A comprehensive evolutionary framework for understanding the maintenance of heritable behavioral variation in humans is yet to be developed. Some evolutionary psychologists have argued that heritable variation will not be found in important, fitness-relevant characteristics because of the winnowing effect of natural selection. This article propounds the opposite view. Heritable variation is ubiquitous in all species, and there are a number of frameworks for understanding its persistence. The author argues that each of the Big Five dimensions of human personality can be seen as the result of a trade-off between different fitness costs and benefits. As there is no unconditionally optimal value of these trade-offs, it is to be expected that genetic diversity will be retained in the population.
Address University of Newcastle, Newcastle, United Kingdom. daniel.nettle@ncl.ac.uk
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0003-066X ISBN Medium
Area Expedition Conference
Notes PMID:16953749 Approved no
Call Number Equine Behaviour @ team @ Serial 4105
Permanent link to this record
 

 
Author Morley, K.I.; Montgomery, G.W.
Title The genetics of cognitive processes: candidate genes in humans and animals Type Journal Article
Year 2001 Publication Behavior Genetics Abbreviated Journal Behav Genet
Volume 31 Issue (down) 6 Pages 511-531
Keywords Animals; *Chromosome Mapping; Drosophila melanogaster; Genetic Markers/*genetics; Humans; Intelligence/*genetics; Mental Retardation/genetics; Mice; Phenotype; Quantitative Trait, Heritable
Abstract It has been hypothesized that numerous genes contribute to individual variation in human cognition. An extensive search of the scientific literature was undertaken to identify candidate genes which might contribute to this complex trait. A list of over 150 candidate genes that may influence some aspect of cognition was compiled. Some genes are particularly strong candidates based on evidence for involvement in cognitive processes in humans, mice, and Drosophila melanogaster. This survey confirms that many genes are associated with cognitive variation and highlights the potential importance of animal models in the study of human cognition.
Address Genetic Epidemiology Laboratory, Queensland Institute of Medical Research, Brisbane, Australia
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0001-8244 ISBN Medium
Area Expedition Conference
Notes PMID:11838530 Approved no
Call Number Equine Behaviour @ team @ Serial 4141
Permanent link to this record
 

 
Author Wallner, B.; Brem, G.; Muller, M.; Achmann, R.
Title Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalskii and Equus caballus Type Journal Article
Year 2003 Publication Animal Genetics Abbreviated Journal Anim Genet
Volume 34 Issue (down) 6 Pages 453-456
Keywords Animals; Base Sequence; DNA, Mitochondrial/genetics; Genetic Variation/*genetics; Horses/classification/*genetics; Male; Molecular Sequence Data; Phylogeny; Probability; Species Specificity; Y Chromosome/*genetics
Abstract The phylogenetic relationship between Equus przewalskii and E. caballus is often a matter of debate. Although these taxa have different chromosome numbers, they do not form monophyletic clades in a phylogenetic tree based on mtDNA sequences. Here we report sequence variation from five newly identified Y chromosome regions of the horse. Two fixed nucleotide differences on the Y chromosome clearly display Przewalski's horse and domestic horse as sister taxa. At both positions the Przewalski's horse haplotype shows the ancestral state, in common with the members of the zebra/ass lineage. We discuss the factors that may have led to the differences in mtDNA and Y-chromosomal observations.
Address Institut fur Tierzucht und Genetik, Veterinarmedizinische Universitat Wien, Veterinarplatz, Wien, Austria. wallner@i122server.vu-wien.ac.at
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0268-9146 ISBN Medium
Area Expedition Conference
Notes PMID:14687077 Approved no
Call Number Equine Behaviour @ team @ Serial 5038
Permanent link to this record