Records |
Author |
Gasser, R.B.; Hung, G.-C.; Chilton, N.B.; Beveridge, I. |
Title |
Advances in developing molecular-diagnostic tools for strongyloid nematodes of equids: fundamental and applied implications |
Type |
Journal Article |
Year |
2004 |
Publication |
Molecular and Cellular Probes |
Abbreviated Journal |
Mol Cell Probes |
Volume |
18 |
Issue |
1 |
Pages |
3-16 |
Keywords |
Animals; DNA, Helminth; DNA, Ribosomal/analysis; Equidae/*parasitology; Molecular Diagnostic Techniques/*methods; Parasitic Diseases, Animal/diagnosis; Strongylida/classification/genetics; Strongylida Infections/*diagnosis/epidemiology/etiology/veterinary |
Abstract |
Infections of equids with parasitic nematodes of the order Strongylida (subfamilies Strongylinae and Cyathostominae) are of major veterinary importance. In last decades, the widespread use of drugs against these parasites has led to problems of resistance within the Cyathostominae, and to an increase in their prevalence and intensity of infection. Novel control strategies, based on improved knowledge of parasite biology and epidemiology, have thus become important. However, there are substantial limitations in the understanding of fundamental biological and systematic aspects of these parasites, which have been due largely to limitations in their specific identification and diagnosis using traditional, morphological approaches. Recently, there has been progress in the development of DNA-based approaches for the specific identification of strongyloids of equids for systematic studies and disease diagnosis. The present article briefly reviews information on the classification, biology, pathogenesis, epidemiology of equine strongyloids and the diagnosis of infections, highlights knowledge gaps in these areas, describes recent advances in the use of molecular techniques for the genetic characterisation, specific identification and differentiation of strongyloids of equids as a basis for fundamental investigations of the systematics, population biology and ecology. |
Address |
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. robinbg@unimelb.edu.au |
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0890-8508 |
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PMID:15036364 |
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Equine Behaviour @ team @ |
Serial |
2636 |
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Author |
Piro, M.; Benjouad, A.; Karom, A.; Nabich, A.; Benbihi, N.; El Allali, K.; Machmoum, M.; Ouragh, L. |
Title |
Genetic Structure of Severe Combined Immunodeficiency Carrier Horses in Morocco Inferred by Microsatellite Data |
Type |
Journal Article |
Year |
2011 |
Publication |
Journal of Equine Veterinary Science |
Abbreviated Journal |
J. Equine Vet. Sci. |
Volume |
31 |
Issue |
11 |
Pages |
618-624 |
Keywords |
Scid; Arab horses; Arab-Barb horses; Microsatellite; Dna; Genetic structure |
Abstract |
A total of 17 microsatellite deoxyribonucleic acid loci used routinely for horse parentage control were used to evaluate genetic diversity among normal Arabian horses and severe combined immunodeficiency (SCID) carrier Arabian horses (ArS) and normal Arab-Barb horses and SCID carrier Arab-Barb horses (ArbeS). On the basis of the genotype of 186 horses, mean allelic diversity was estimated as 6.82, 5.53, and 6.7059 in normal Arabian horses, ArS, and for both groups of Arab-Barb horses, respectively. Five specific alleles were observed in ArS and ArbeS, with one common with ArS at HMS6, whereas five alleles common between ArS and ArbeS had a high frequency. Expected and observed heterozygosity showed great heterogeneity in the population studied and were similar or higher when compared with other studies on Arabian horses. Coefficient of gene differentiation Gst of Nei associated with Nei's genetic distance and multivariate correspondence analysis indicated a possible differentiation between the studied populations when analyzed separately according to breed. Probability of assignment of a horse to a specific group was assessed using a full and partial Bayesian approach. In all, 80.6% of Arab horses and 78.2% of Arab-Barb horses were assigned properly with a partial Bayesian test, which provided better results than the full one. These findings will be useful for identification of SCID carrier horses by using the microsatellite deoxyribonucleic acid loci used routinely for horse parentage control in our laboratory. |
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ISSN ![sorted by ISSN field, descending order (down)](img/sort_desc.gif) |
0737-0806 |
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Equine Behaviour @ team @ |
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6657 |
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Author |
Birch, H.L.; Bailey, A.J.; Goodship, A.E. |
Title |
Macroscopic 'degeneration' of equine superficial digital flexor tendon is accompanied by a change in extracellular matrix composition |
Type |
Journal Article |
Year |
1998 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
Volume |
30 |
Issue |
6 |
Pages |
534-539 |
Keywords |
Animals; Collagen/analysis; DNA/analysis; Extracellular Matrix/*chemistry; Glycosaminoglycans/analysis; Horses/injuries/*physiology; Immunohistochemistry; Rupture/veterinary; Tendon Injuries/metabolism/pathology/veterinary; Tendons/chemistry/*pathology; Water/analysis |
Abstract |
Injuries to the superficial digital flexor tendon are common in horses required to gallop and jump at speed. Partial rupture of this tendon usually occurs in the central core of the midmetacarpal region and may be preceded by localised degenerative changes. Post mortem examination of apparently normal equine flexor tendons has revealed an abnormal macroscopic appearance in the central core, characterised by a reddish discolouration. We have previously shown that there is also physical damage to the collagen fibres. In the present study we tested the hypothesis that the abnormal appearance is accompanied by changes in the composition of the extracellular matrix of the tendon. Biochemical analysis of the extracellular matrix demonstrated an increase in total sulphated glycosaminoglycan content, increase in the proportion of type III collagen and decrease in collagen linked fluorescence in the central core of 'degenerated' tendons relative to tissue from the peripheral region of the same tendon. Dry matter content and total collagen content were not significantly different between tendon zones or normal and 'degenerated' tendons. These changes suggest a change in cell metabolism and matrix turnover in the central core of the tendon and are likely to contribute to a decrease in mechanical properties in this part of the tendon, predisposing to the characteristic partial rupture of the tendon. |
Address |
Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hatfield, UK |
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English |
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ISSN ![sorted by ISSN field, descending order (down)](img/sort_desc.gif) |
0425-1644 |
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PMID:9844973 |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
3794 |
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Author |
Boucher, J.M.; Hanosset, R.; Augot, D.; Bart, J.M.; Morand, M.; Piarroux, R.; Pozet-Bouhier, F.; Losson, B.; Cliquet, F. |
Title |
Detection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form |
Type |
Journal Article |
Year |
2005 |
Publication |
Veterinary Parasitology |
Abbreviated Journal |
Vet Parasitol |
Volume |
129 |
Issue |
3-4 |
Pages |
259-266 |
Keywords |
Animals; Base Sequence; DNA, Helminth/chemistry/genetics; Echinococcosis/parasitology/pathology/*veterinary; Echinococcus multilocularis/*isolation & purification; Electron Transport Complex IV/chemistry/genetics; France; Histocytochemistry/veterinary; Liver/parasitology/pathology; Male; Molecular Sequence Data; Polymerase Chain Reaction/veterinary; Sequence Alignment; Sus scrofa/*parasitology; Swine Diseases/*parasitology/pathology |
Abstract |
Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are known to host incomplete development of larval E. multilocularis. We report a case of E. multilocularis portage in two wild boars from a high endemic area in France (Department of Jura). Histological examination was performed and the DNA was isolated from hepatic lesions then amplified by using three PCR methods in two distinct institutes. Molecular characterisation of PCR products revealed 99% nucleotide sequence homology with the specific sequence of the U1 sn RNA gene of E. multilocularis, 99 and 99.9% nucleotide sequence homology with the specific sequence of the cytochrome oxydase gene of Echinococcus genus and 99.9% nucleotide sequence homology with a genomic DNA sequence of Echinococcus genus for the first and the second wild boar, respectively. |
Address |
AFSSA Nancy, Laboratoire d'Etudes et de Recherches sur la Rage et la Pathologie des Animaux Sauvages, Domaine de Pixerecourt-B.P. 9, Malzeville F 54220, France |
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ISSN ![sorted by ISSN field, descending order (down)](img/sort_desc.gif) |
0304-4017 |
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Notes |
PMID:15845281 |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2629 |
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Author |
Ishida, N.; Hirano, T.; Mukoyama, H. |
Title |
Detection of aberrant alleles in the D-loop region of equine mitochondrial DNA by single-strand conformation polymorphism (SSCP) analysis |
Type |
Journal Article |
Year |
1994 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
25 |
Issue |
4 |
Pages |
287 |
Keywords |
*Alleles; Animals; Base Sequence; *DNA, Mitochondrial; DNA, Single-Stranded/genetics; Female; Gene Frequency; Genomic Imprinting; Horses/*genetics; Male; Molecular Sequence Data; Pedigree; *Polymorphism, Genetic |
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Address |
Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo |
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0268-9146 |
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PMID:7985852 |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2213 |
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Author |
Breen, M.; Downs, P.; Irvin, Z.; Bell, K. |
Title |
Intrageneric amplification of horse microsatellite markers with emphasis on the Przewalski's horse (E. przewalskii) |
Type |
Journal Article |
Year |
1994 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
25 |
Issue |
6 |
Pages |
401-405 |
Keywords |
Animals; DNA, Satellite/*genetics; *Gene Amplification; Gene Frequency; *Genetic Markers; Heterozygote; Horses/*genetics; Species Specificity |
Abstract |
Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was calculated to be 0.9994. The results suggest that it should be possible to use E. caballus-derived microsatellite markers to provide parentage verification and additional valuable information to the captive management of E. przewalskii. The ability to amplify corresponding loci in the remaining five species of the genus was also confirmed, illustrating the general application of markers isolated from the domestic horse to the evaluation of polymorphism in the other six species of the genus. |
Address |
Australian Equine Blood Typing Research Laboratory, University of Queensland, St Lucia |
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English |
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0268-9146 |
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PMID:7695120 |
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no |
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Equine Behaviour @ team @ |
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2246 |
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Author |
Wallner, B.; Brem, G.; Muller, M.; Achmann, R. |
Title |
Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalskii and Equus caballus |
Type |
Journal Article |
Year |
2003 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
34 |
Issue |
6 |
Pages |
453-456 |
Keywords |
Animals; Base Sequence; DNA, Mitochondrial/genetics; Genetic Variation/*genetics; Horses/classification/*genetics; Male; Molecular Sequence Data; Phylogeny; Probability; Species Specificity; Y Chromosome/*genetics |
Abstract |
The phylogenetic relationship between Equus przewalskii and E. caballus is often a matter of debate. Although these taxa have different chromosome numbers, they do not form monophyletic clades in a phylogenetic tree based on mtDNA sequences. Here we report sequence variation from five newly identified Y chromosome regions of the horse. Two fixed nucleotide differences on the Y chromosome clearly display Przewalski's horse and domestic horse as sister taxa. At both positions the Przewalski's horse haplotype shows the ancestral state, in common with the members of the zebra/ass lineage. We discuss the factors that may have led to the differences in mtDNA and Y-chromosomal observations. |
Address |
Institut fur Tierzucht und Genetik, Veterinarmedizinische Universitat Wien, Veterinarplatz, Wien, Austria. wallner@i122server.vu-wien.ac.at |
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0268-9146 |
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PMID:14687077 |
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no |
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Equine Behaviour @ team @ |
Serial |
5038 |
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Author |
Oakenfull, E.A.; Ryder, O.A. |
Title |
Mitochondrial control region and 12S rRNA variation in Przewalski's horse (Equus przewalskii) |
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Journal Article |
Year |
1998 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
29 |
Issue |
6 |
Pages |
456-459 |
Keywords |
Animals; DNA, Mitochondrial/*genetics; Female; *Genetic Variation; Horses/*genetics; Male; Pedigree; RNA, Ribosomal/*genetics |
Abstract |
Variation in the control region and the 12S rRNA gene of all surviving mitochondrial lineages of Przewalski's horse was investigated. Variation is low despite the present day population being descended from 13 individuals probably representing animals from three different regions of its range. Phylogenetic comparison of these sequences, with sequences for the domestic horse, does not resolve the ancestral status of either horse. |
Address |
Center for Reproduction of Endangered Species, Zoological Society of San Diego, CA 92112, USA |
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0268-9146 |
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PMID:9883508 |
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no |
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Equine Behaviour @ team @ |
Serial |
5040 |
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Author |
Nicol, C.J.; Yoon, M.; Ward, J.M.; Yamashita, M.; Fukamachi, K.; Peters, J.M.; Gonzalez, F.J. |
Title |
PPARgamma influences susceptibility to DMBA-induced mammary, ovarian and skin carcinogenesis |
Type |
Journal Article |
Year |
2004 |
Publication |
Carcinogenesis |
Abbreviated Journal |
Carcinogenesis |
Volume |
25 |
Issue |
9 |
Pages |
1747-1755 |
Keywords |
9,10-Dimethyl-1,2-benzanthracene/*toxicity; Animals; DNA Primers/chemistry; Disease Susceptibility; Female; Heterozygote; Humans; Mammary Neoplasms, Experimental/chemically induced/*pathology; Mice; Ovarian Neoplasms/chemically induced/*pathology; RNA, Messenger/genetics/metabolism; Receptors, Cytoplasmic and Nuclear/genetics/*physiology; Reverse Transcriptase Polymerase Chain Reaction; Skin Neoplasms/chemically induced/*pathology; Survival Rate; Transcription Factors/genetics/*physiology; Zinc Fingers |
Abstract |
Peroxisome proliferator-activated receptor gamma (PPARgamma), a member of the nuclear receptor superfamily, plays a role in adipocyte differentiation, type II diabetes, macrophage response to inflammation and is suggested to influence carcinogen-induced colon cancer. Studies done in vitro and in vivo also revealed that PPARgamma ligands might promote differentiation and/or regression of mammary tumors. To directly evaluate the role of PPARgamma in mammary carcinogenesis, PPARgamma wild-type (+/+) or heterozygous (+/-) mice were administered 1 mg 7,12-dimethylbenz[a]anthracene (DMBA) by gavage once a week for 6 weeks and followed for a total of 25 weeks. Compared with congenic PPARgamma(+/+) littermate controls, PPARgamma(+/-) mice had early evidence for increased susceptibility to DMBA-mediated carcinogenesis based on a 1.6-fold increase in the percentage of mice with skin papillomas, as well as a 1.7-fold increase in the numbers of skin papillomas per mouse (P < 0.05). Similarly, PPARgamma(+/-) mice also had a 1.5-fold decreased survival rate (P = 0.059), and a 1.7-fold increased incidence of total tumors per mouse (P < 0.01). Moreover, PPARgamma(+/-) mice had an almost 3-fold increase in mammary adenocarcinomas (P < 0.05), an over 3-fold increase in ovarian granulosa cell carcinomas (P < 0.05), an over 3-fold increase in malignant tumors (P < 0.02) and a 4.6-fold increase in metastatic incidence. These results are the first to demonstrate an increased susceptibility in vivo of PPARgamma haploinsufficiency to DMBA-mediated carcinogenesis and suggest that PPARgamma may act as a tumor modifier of skin, ovarian and breast cancers. The data also support evidence suggesting a beneficial role for PPARgamma-specific ligands in the chemoprevention of mammary, ovarian and skin carcinogenesis. |
Address |
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA |
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0143-3334 |
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PMID:15073042 |
Approved |
no |
Call Number |
refbase @ user @ |
Serial |
76 |
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Author |
Muscatello, G.; Anderson, G.A.; Gilkerson, J.R.; Browning, G.F. |
Title |
Associations between the ecology of virulent Rhodococcus equi and the epidemiology of R. equi pneumonia on Australian thoroughbred farms |
Type |
Journal Article |
Year |
2006 |
Publication |
Applied and Environmental Microbiology |
Abbreviated Journal |
Appl Environ Microbiol |
Volume |
72 |
Issue |
9 |
Pages |
6152-6160 |
Keywords |
Actinomycetales Infections/epidemiology/microbiology/*veterinary; Air Microbiology; Animal Husbandry; Animals; Animals, Newborn; Australia/epidemiology; Colony Count, Microbial; DNA, Bacterial/genetics; Ecosystem; Horse Diseases/epidemiology/*microbiology; Horses; Pneumonia, Bacterial/epidemiology/microbiology/*veterinary; Rhodococcus equi/genetics/isolation & purification/*pathogenicity; Soil Microbiology; Virulence |
Abstract |
The ecology of virulent strains of Rhodococcus equi on horse farms is likely to influence the prevalence and severity of R. equi pneumonia in foals. This study examined the association between the ecology of virulent R. equi and the epidemiology of R. equi pneumonia by collecting air and soil samples over two breeding seasons (28 farm-year combinations) on Thoroughbred breeding farms with different reported prevalences of R. equi pneumonia. Colony blotting and DNA hybridization were used to detect and measure concentrations of virulent R. equi. The prevalence of R. equi pneumonia was associated with the airborne burden of virulent R. equi (both the concentration and the proportion of R. equi bacteria that were virulent) but was not associated with the burden of virulent R. equi in the soil. Univariable screening and multivariable model building were used to evaluate the effect of environmental and management factors on virulent R. equi burdens. Lower soil moisture concentrations and lower pasture heights were significantly associated with elevated airborne concentrations of virulent R. equi, as were the holding pens and lanes, which typically were sandy, dry, and devoid of pasture cover. Few variables appeared to influence concentrations of virulent R. equi in soil. Acidic soil conditions may have contributed to an elevated proportion of virulent strains within the R. equi population. Environmental management strategies that aim to reduce the level of exposure of susceptible foals to airborne virulent R. equi are most likely to reduce the impact of R. equi pneumonia on endemically affected farms. |
Address |
School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia. mug@unimelb.edu.au |
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ISSN ![sorted by ISSN field, descending order (down)](img/sort_desc.gif) |
0099-2240 |
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PMID:16957241 |
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no |
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Equine Behaviour @ team @ |
Serial |
2622 |
Permanent link to this record |