Records |
Author |
Birch, H.L.; Bailey, A.J.; Goodship, A.E. |
Title |
Macroscopic 'degeneration' of equine superficial digital flexor tendon is accompanied by a change in extracellular matrix composition |
Type |
Journal Article |
Year |
1998 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
Volume |
30 |
Issue |
6 |
Pages |
534-539 |
Keywords |
Animals; Collagen/analysis; DNA/analysis; Extracellular Matrix/*chemistry; Glycosaminoglycans/analysis; Horses/injuries/*physiology; Immunohistochemistry; Rupture/veterinary; Tendon Injuries/metabolism/pathology/veterinary; Tendons/chemistry/*pathology; Water/analysis |
Abstract |
Injuries to the superficial digital flexor tendon are common in horses required to gallop and jump at speed. Partial rupture of this tendon usually occurs in the central core of the midmetacarpal region and may be preceded by localised degenerative changes. Post mortem examination of apparently normal equine flexor tendons has revealed an abnormal macroscopic appearance in the central core, characterised by a reddish discolouration. We have previously shown that there is also physical damage to the collagen fibres. In the present study we tested the hypothesis that the abnormal appearance is accompanied by changes in the composition of the extracellular matrix of the tendon. Biochemical analysis of the extracellular matrix demonstrated an increase in total sulphated glycosaminoglycan content, increase in the proportion of type III collagen and decrease in collagen linked fluorescence in the central core of 'degenerated' tendons relative to tissue from the peripheral region of the same tendon. Dry matter content and total collagen content were not significantly different between tendon zones or normal and 'degenerated' tendons. These changes suggest a change in cell metabolism and matrix turnover in the central core of the tendon and are likely to contribute to a decrease in mechanical properties in this part of the tendon, predisposing to the characteristic partial rupture of the tendon. |
Address |
Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hatfield, UK |
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English |
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Edition |
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ISSN |
0425-1644 |
ISBN |
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Notes |
PMID:9844973 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
3794 |
Permanent link to this record |
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Author |
Oakenfull, E.A.; Ryder, O.A. |
Title |
Mitochondrial control region and 12S rRNA variation in Przewalski's horse (Equus przewalskii) |
Type |
Journal Article |
Year |
1998 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
29 |
Issue |
6 |
Pages |
456-459 |
Keywords |
Animals; DNA, Mitochondrial/*genetics; Female; *Genetic Variation; Horses/*genetics; Male; Pedigree; RNA, Ribosomal/*genetics |
Abstract |
Variation in the control region and the 12S rRNA gene of all surviving mitochondrial lineages of Przewalski's horse was investigated. Variation is low despite the present day population being descended from 13 individuals probably representing animals from three different regions of its range. Phylogenetic comparison of these sequences, with sequences for the domestic horse, does not resolve the ancestral status of either horse. |
Address |
Center for Reproduction of Endangered Species, Zoological Society of San Diego, CA 92112, USA |
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English |
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Series Editor |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0268-9146 |
ISBN |
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Notes |
PMID:9883508 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
5040 |
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Author |
Wallner, B.; Brem, G.; Muller, M.; Achmann, R. |
Title |
Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalskii and Equus caballus |
Type |
Journal Article |
Year |
2003 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
34 |
Issue |
6 |
Pages |
453-456 |
Keywords |
Animals; Base Sequence; DNA, Mitochondrial/genetics; Genetic Variation/*genetics; Horses/classification/*genetics; Male; Molecular Sequence Data; Phylogeny; Probability; Species Specificity; Y Chromosome/*genetics |
Abstract |
The phylogenetic relationship between Equus przewalskii and E. caballus is often a matter of debate. Although these taxa have different chromosome numbers, they do not form monophyletic clades in a phylogenetic tree based on mtDNA sequences. Here we report sequence variation from five newly identified Y chromosome regions of the horse. Two fixed nucleotide differences on the Y chromosome clearly display Przewalski's horse and domestic horse as sister taxa. At both positions the Przewalski's horse haplotype shows the ancestral state, in common with the members of the zebra/ass lineage. We discuss the factors that may have led to the differences in mtDNA and Y-chromosomal observations. |
Address |
Institut fur Tierzucht und Genetik, Veterinarmedizinische Universitat Wien, Veterinarplatz, Wien, Austria. wallner@i122server.vu-wien.ac.at |
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English |
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Series Issue |
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Edition |
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ISSN |
0268-9146 |
ISBN |
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Conference |
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Notes |
PMID:14687077 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
5038 |
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Author |
Zhao, C.J.; Qin, Y.H.; Lee, X.H.; Wu, C. |
Title |
Molecular and cytogenetic paternity testing of a male offspring of a hinny |
Type |
Journal Article |
Year |
2006 |
Publication |
Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie |
Abbreviated Journal |
J Anim Breed Genet |
Volume |
123 |
Issue |
6 |
Pages |
403-405 |
Keywords |
Animals; Cytogenetic Analysis; DNA, Mitochondrial/genetics; Equidae/*genetics; Female; Horses/genetics; Hybridization, Genetic; Male; Microsatellite Repeats; Pedigree; Protamines/genetics; Sexual Behavior, Animal |
Abstract |
An alleged male foal of a female mule, whose sire and grandparents were unknown, was identified for its pedigree. Parentage testing was conducted by comparing polymorphism of 12 microsatellite DNA sites and mitochondrial D-loop sequences of the male foal and the female mule. Both the sequence analysis of species-specific DNA fragments and a cytogenetic analysis were performed to identify the species of the foal and its parents. The results showed that the alleged female mule is actually a hinny, and the male foal, which possesses 62 chromosomes, qualifies as an offspring of the female hinny and a jack donkey. |
Address |
Equine Center, China Agricultural University, Beijing, China |
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English |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0931-2668 |
ISBN |
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Medium |
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Conference |
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Notes |
PMID:17177697 |
Approved |
no |
Call Number |
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Serial |
1846 |
Permanent link to this record |
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Author |
Breen, M.; Downs, P.; Irvin, Z.; Bell, K. |
Title |
Intrageneric amplification of horse microsatellite markers with emphasis on the Przewalski's horse (E. przewalskii) |
Type |
Journal Article |
Year |
1994 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
25 |
Issue |
6 |
Pages |
401-405 |
Keywords |
Animals; DNA, Satellite/*genetics; *Gene Amplification; Gene Frequency; *Genetic Markers; Heterozygote; Horses/*genetics; Species Specificity |
Abstract |
Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was calculated to be 0.9994. The results suggest that it should be possible to use E. caballus-derived microsatellite markers to provide parentage verification and additional valuable information to the captive management of E. przewalskii. The ability to amplify corresponding loci in the remaining five species of the genus was also confirmed, illustrating the general application of markers isolated from the domestic horse to the evaluation of polymorphism in the other six species of the genus. |
Address |
Australian Equine Blood Typing Research Laboratory, University of Queensland, St Lucia |
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English |
Summary Language |
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Series Editor |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0268-9146 |
ISBN |
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Medium |
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Conference |
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Notes |
PMID:7695120 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2246 |
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Author |
Cilnis, M.J.; Kang, W.; Weaver, S.C. |
Title |
Genetic conservation of Highlands J viruses |
Type |
Journal Article |
Year |
1996 |
Publication |
Virology |
Abbreviated Journal |
Virology |
Volume |
218 |
Issue |
2 |
Pages |
343-351 |
Keywords |
Alphavirus/*genetics; Alphavirus Infections/transmission/veterinary/virology; Amino Acid Sequence; Animals; Base Sequence; Conserved Sequence; Disease Outbreaks; Encephalitis, Viral/veterinary/virology; *Evolution, Molecular; Horses; Molecular Sequence Data; Phylogeny; RNA, Viral/genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Turkeys; Variation (Genetics)/*genetics |
Abstract |
We studied molecular evolution of the mosquito-borne alphavirus Highlands J (HJ) virus by sequencing PCR products generated from 19 strains isolated between 1952 and 1994. Sequences of 1200 nucleotides including portions of the E1 gene and the 3' untranslated region revealed a relatively slow evolutionary rate estimated at 0.9-1.6 x 10(-4) substitutions per nucleotide per year. Phylogenetic trees indicated that all HJ viruses descended from a common ancestor and suggested the presence of one dominant lineage in North America. However, two or more minor lineages probably circulated simultaneously for periods of years to a few decades. Strains isolated from a horse suffering encephalitis, and implicated in a recent turkey outbreak, were not phylogenetically distinct from strains isolated in other locations during the same time periods. Our findings are remarkably similar to those we obtained previously for another North American alphavirus, eastern equine encephalomyelitis virus, with which Highlands J shares primary mosquito and avian hosts, geographical distribution, and ecology. These results support the hypotheses that the duration of the transmission season affects arboviral evolutionary rates and vertebrate host mobility influences genetic diversity. |
Address |
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA |
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English |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0042-6822 |
ISBN |
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Medium |
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Area |
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Conference |
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Notes |
PMID:8610461 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2657 |
Permanent link to this record |
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Author |
Feh, C.; Munkhtuya, B. |
Title |
Male infanticide and paternity analyses in a socially natural herd of Przewalski`s horses: Sexual selection? |
Type |
Journal Article |
Year |
2008 |
Publication |
Behavioural Processes |
Abbreviated Journal |
Behav. Process. |
Volume |
78 |
Issue |
3 |
Pages |
335-339 |
Keywords |
DNA paternity analysis; Human disturbance; Male infanticide; Przewalski's horse (Equus ferus przewalskii); Sexual selection |
Abstract |
The sexual selection hypothesis explains infanticide by males in many mammals. In our 11-year study, we investigated this hypothesis in a herd of Przewalski's horses where we had witnessed infanticidal attacks. Infanticide was highly conditional and not simply linked to takeovers. Attacks occurred in only five of 39 cases following a takeover, and DNA paternity revealed that, although infanticidal stallions were not the genetic fathers in four cases out of five, stallions present at birth did not significantly attempt to kill unrelated foals. Infanticide did not reduce birth intervals; only in one case out of five was the infanticidal stallion, the father of the next foal; mothers whose foals were attacked subsequently avoided associating with infanticidal stallions. Therefore, evidence for the sexual selection hypothesis was weak. The “human disturbance” hypothesis received some support, as only zoo bred stallions which grew up in unnatural social groups attacked foals of mares which were pregnant during takeovers. |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4632 |
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Author |
Ishida, N.; Hirano, T.; Mukoyama, H. |
Title |
Detection of aberrant alleles in the D-loop region of equine mitochondrial DNA by single-strand conformation polymorphism (SSCP) analysis |
Type |
Journal Article |
Year |
1994 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
25 |
Issue |
4 |
Pages |
287 |
Keywords |
*Alleles; Animals; Base Sequence; *DNA, Mitochondrial; DNA, Single-Stranded/genetics; Female; Gene Frequency; Genomic Imprinting; Horses/*genetics; Male; Molecular Sequence Data; Pedigree; *Polymorphism, Genetic |
Abstract |
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Address |
Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo |
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English |
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ISSN |
0268-9146 |
ISBN |
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Notes |
PMID:7985852 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2213 |
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Author |
Passler, S.; Pfeffer, M. |
Title |
Detection of antibodies to alphaviruses and discrimination between antibodies to eastern and western equine encephalitis viruses in rabbit sera using a recombinant antigen and virus-specific monoclonal antibodies |
Type |
Journal Article |
Year |
2003 |
Publication |
Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health |
Abbreviated Journal |
J Vet Med B Infect Dis Vet Public Health |
Volume |
50 |
Issue |
6 |
Pages |
265-269 |
Keywords |
Animals; Antibodies, Monoclonal/*immunology; Antibodies, Viral/*analysis/blood; DNA Primers; Encephalitis Virus, Eastern Equine/genetics/*immunology; Encephalitis Virus, Western Equine/genetics/*immunology; Encephalomyelitis, Equine/*diagnosis/*virology; Epitopes; Fluorescent Antibody Technique/*veterinary; Horses; Rabbits; Recombination, Genetic; Reverse Transcriptase Polymerase Chain Reaction/veterinary |
Abstract |
Three arthropod-borne alphaviruses, western equine encephalitis viruses (WEEV), eastern equine encephalitis viruses (EEEV) and Venezuelan equine encephalitis viruses are the aetiological agents of a sometimes severe encephalomyelitis in equines and humans in the New World. With regard to the different ecology and epidemiology of these viruses, a method applied in serological screening should be able to distinguish between them as well as other related members of the genus Alphavirus in the American continent. However, this has been hampered in the past by (a) the close antigenic relationship between alphaviruses in traditional serological assays, especially in the routinely used haemagglutination-inhibition, and (b) the need of biosafety level 3 facilities to grow the viral antigens. An epitope blocking assay using an EEEV glycoprotein E1-expressing recombinant Sindbis virus and virus-specific monoclonal antibodies (mAbs) binding to the E1 of EEEV (strain NJ/60) and the E1 of Sindbis virus was established using automated flow cytometry. The test was evaluated using sera of infected and vaccinated rabbits. A cut-off value of 30% inhibition for antigenic complex-specific seroconversion was found to be sufficient for the detection of the respective infection. By using three different mAbs in parallel, we were able to detect alphavirus genus-, EEEV- and WEEV-complex-specific serum antibodies. As this test is based on the inhibition of binding of virus-specific mAbs, sera of every origin other than mouse can be tested. Thus, this assay may prove useful in the serological screening of a variety of animal species during an outbreak investigation. |
Address |
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany |
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English |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0931-1793 |
ISBN |
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Notes |
PMID:14628996 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2639 |
Permanent link to this record |
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Author |
Boucher, J.M.; Hanosset, R.; Augot, D.; Bart, J.M.; Morand, M.; Piarroux, R.; Pozet-Bouhier, F.; Losson, B.; Cliquet, F. |
Title |
Detection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form |
Type |
Journal Article |
Year |
2005 |
Publication |
Veterinary Parasitology |
Abbreviated Journal |
Vet Parasitol |
Volume |
129 |
Issue |
3-4 |
Pages |
259-266 |
Keywords |
Animals; Base Sequence; DNA, Helminth/chemistry/genetics; Echinococcosis/parasitology/pathology/*veterinary; Echinococcus multilocularis/*isolation & purification; Electron Transport Complex IV/chemistry/genetics; France; Histocytochemistry/veterinary; Liver/parasitology/pathology; Male; Molecular Sequence Data; Polymerase Chain Reaction/veterinary; Sequence Alignment; Sus scrofa/*parasitology; Swine Diseases/*parasitology/pathology |
Abstract |
Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are known to host incomplete development of larval E. multilocularis. We report a case of E. multilocularis portage in two wild boars from a high endemic area in France (Department of Jura). Histological examination was performed and the DNA was isolated from hepatic lesions then amplified by using three PCR methods in two distinct institutes. Molecular characterisation of PCR products revealed 99% nucleotide sequence homology with the specific sequence of the U1 sn RNA gene of E. multilocularis, 99 and 99.9% nucleotide sequence homology with the specific sequence of the cytochrome oxydase gene of Echinococcus genus and 99.9% nucleotide sequence homology with a genomic DNA sequence of Echinococcus genus for the first and the second wild boar, respectively. |
Address |
AFSSA Nancy, Laboratoire d'Etudes et de Recherches sur la Rage et la Pathologie des Animaux Sauvages, Domaine de Pixerecourt-B.P. 9, Malzeville F 54220, France |
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English |
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Edition |
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ISSN |
0304-4017 |
ISBN |
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Notes |
PMID:15845281 |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2629 |
Permanent link to this record |