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Author |
Dabareiner, R.M.; Sullins, K.E.; White, N.A. 2nd |
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Title |
Progression of femoropatellar osteochondrosis in nine young horses. Clinical, radiographic and arthroscopic findings |
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Journal Article |
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Year |
1993 |
Publication |
Veterinary Surgery : VS : the Official Journal of the American College of Veterinary Surgeons |
Abbreviated Journal |
Vet Surg |
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Volume |
22 |
Issue |
6 |
Pages |
515-523 |
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Keywords |
Animals; Arthroscopy/veterinary; Debridement/veterinary; Exudates and Transudates; Female; Femur; Follow-Up Studies; Horse Diseases/*diagnosis/radiography/surgery; Horses; Lameness, Animal/*etiology; Male; Osteochondritis/diagnosis/radiography/surgery/*veterinary; Patella; Stifle; Treatment Outcome |
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Abstract |
The clinical and radiographic progression, and arthroscopic findings for nine young horses (< 1 year of age) with femoropatellar osteochondrosis (OCD) are presented. Horses had a 2 to 12 week history of bilateral (8 horses) or unilateral (1 horse) hindlimb lameness. The most consistent clinical signs included femoropatellar joint distention and bilateral hindlimb lameness. At the onset of clinical signs, radiographic lesions were not present (4 horses) or subtle (5 horses), but were easily identified on radiographs taken 4 to 24 weeks later. Arthroscopic surgery was delayed until radiographic changes became obvious. Surgical findings in 20 femoropatellar joints were most commonly osteochondral “flaps” located on the proximal lateral trochlear ridge of the femur and were larger than had been indicated by the radiographs. Eight horses were being used for their intended purpose, which was racing (3 horses were racing and 3 were in race training), dressage (1 horse) or pleasure riding (1 horse). One horse required a second surgery when similar lesions developed on the opposite stifle, and was euthanatized 2 months later because of persistent lameness. One clinical signs are observed, osteochondrosis lesions of the distal femur can progress in foals younger than 9 months of age and the full extent of the radiographic lesion may take several weeks to develop. |
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Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Leesburg, Virginia |
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English |
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0161-3499 |
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PMID:8116209 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3748 |
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Author |
Petter-Puchner, A.H.; Froetscher, W.; Krametter-Froetscher, R.; Lorinson, D.; Redl, H.; van Griensven, M. |
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Title |
The long-term neurocompatibility of human fibrin sealant and equine collagen as biomatrices in experimental spinal cord injury |
Type |
Journal Article |
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Year |
2007 |
Publication |
Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft fur Toxikologische Pathologie |
Abbreviated Journal |
Exp Toxicol Pathol |
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Volume |
58 |
Issue |
4 |
Pages |
237-245 |
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Keywords |
Animals; Axotomy; Biocompatible Materials/*therapeutic use; Collagen/*therapeutic use; Fibrin Tissue Adhesive/*therapeutic use; Horses; Humans; Immunohistochemistry; Male; Motor Activity/physiology; Nerve Regeneration/*physiology; Rats; Recovery of Function; Spinal Cord/pathology/physiology; Spinal Cord Injuries/pathology/*therapy; Thoracic Vertebrae |
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Abstract |
INTRODUCTION: While fibrin sealant (FS) and equine collagen (EC) have been used as scaffold materials in experimental spinal cord injury (SCI), questions concerning neurocompatibility still remain. In this study, we assessed potential adverse effects, as well as functional and histological impact of FS and EC in subtotal hemisection of the thoracic spinal cord (SC) in rats. METHODS: 124 male rats were randomly assigned to four main groups (n=31): Sham (SH), Lesion only (L), fibrin sealant (GFS) and equine collagen group (GEC). SH animals received laminectomy only; all other animals underwent subtotal lateral hemisection at T9. Treatment consisted of application of FS or EC into the lesion gap in GFS and GEC, which was left empty in L. GFS, GEC, L and SH were each further divided into 4 subgroups: One subgroup, consisting of 10 rats was subjected to behavioural and reflex testing before surgery and followed up on days 1,7, 14, 21, 28 post op and then sacrificed. Haemalaun or cresyl violet (CV) was used to identify neutrophils in parasagittal cord sections which were obtained on day 1 (n=7). Sections stained for quantification of microglia/macrophages using ED-1 on day 3 (n=7), day 7 (n=7) and day 28 (n=7 out of 10). Additionally, neural filament (NF) staining was chosen to detect axonal regeneration and the length of ingrowth into FS and EC, Luxol blue for myelination, Von Willebrand factor for vascularisation, and glial fibrillary acidic protein (GFAP) staining for detection of astrocytes in glial scars on day 28. RESULTS: No adverse effects were observed in the treatment groups. Compared to L, GFS and GEC performed significantly better in the Basso, Beattie, Bresnahan (BBB) score and hopping responses. Proprioceptive placing was markedly improved in FS and EC compared to L. Axonal regrowth was found in GFS and GEC--the regrowth in the GFS was accompanied by myelination and vascularisation. Glial scarring occurred in all groups. Discussion Both biomatrices improved functional recovery compared to L and no adverse effects were perceived. |
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Ludwig Boltzmann Institute of Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200-Vienna, Austria |
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0940-2993 |
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Notes |
PMID:17118635 |
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Serial |
1852 |
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Author |
Zeitler-Feicht, M.H. |
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Title |
[Critical consideration of the “Guideline for the Evaluation of Raising Horses” and keeping horses outside in the winter] |
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Journal Article |
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Year |
2004 |
Publication |
DTW. Deutsche Tierarztliche Wochenschrift |
Abbreviated Journal |
Dtsch Tierarztl Wochenschr |
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Volume |
111 |
Issue |
3 |
Pages |
120-123 |
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Keywords |
Animal Feed/standards; Animal Husbandry/legislation & jurisprudence/methods/*standards; *Animal Welfare/legislation & jurisprudence; Animals; Cold; Female; Germany; Guidelines/*standards; Horses/*physiology; Housing, Animal/legislation & jurisprudence/*standards; Legislation, Veterinary; Male; Seasons |
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The guidelines of the Federal Ministry of User Protection, Nutrition and Agriculture (BMVEL) regarding “horse keeping with respect to animal welfare” are from 1995 (BMELF, 1995). Therefore, they are not suitable for modern horse keeping. The Veterinary Association for Animal Welfare (TVT) held it to be necessary to rework the guide-lines in light of 1) many subsequent investigations concerning horse keeping, and 2) the species-specific needs of horses in practice. Each chapter of the BMELF (1995) guide-lines was revised such that the literature and practical experiences were updated. Several chapters (recumbency resting behaviour, fences, underground outdoor and in stables, litter) were added in the position paper of the TVT to reflect the increasing use of boxes with paddocks, loose housing systems with open yards, pasture and winter yards as housing conditions. Keeping horses outdoors permanently during winter is possible because horses have very good thermoregulatory capabilities so that they are able to adapt themselves to cold conditions. However, in light of animal welfare, the holding system must include adequate shelter (natural or artificial). Shelters should protect against wetness, heat, cold and wind, and must be sufficiently large and high, with a dry and clean underground. In keeping horses outdoors permanently, the paths to the feeding and watering areas and to the shelter must be dry. The food must also be protected against mould and soiling. Keeping horses permanently without adequate shelter or in deep marsh without any dry places is against the Animal Protection Act. |
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Address |
Lehrgebiet fur Tierhaltung und Verhaltenskunde, Wissenschaftszentrum Weihenstephan fur Ernahrung, Landnutzung und Umwelt, Technische Universitat Munchen. Zeitler-Feicht@tz.agrar.tu-muenchen.de |
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German |
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Kritische Betrachtung der “Leitlinien zur Beurteilung von Pferdehaltungen” und Winteraussenhaltung von Pferden |
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0341-6593 |
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Notes |
PMID:15195962 |
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no |
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Serial |
1900 |
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Author |
Momozawa, Y.; Takeuchi, Y.; Tozaki, T.; Kikusui, T.; Hasegawa, T.; Raudsepp, T.; Chowdhary, B.P.; Kusunose, R.; Mori, Y. |
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Title |
SNP detection and radiation hybrid mapping in horses of nine candidate genes for temperament |
Type |
Journal Article |
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Year |
2007 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
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Volume |
38 |
Issue |
1 |
Pages |
81-83 |
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Keywords |
Animals; *Behavior, Animal; Breeding; Horses/*genetics/physiology; *Polymorphism, Single Nucleotide; Radiation Hybrid Mapping; *Temperament |
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Laboratory of Veterinary Ethology, The University of Tokyo, Tokyo 113-8657, Japan |
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ISSN |
0268-9146 |
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Notes |
PMID:17257195 |
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Call Number |
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Serial |
1834 |
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Author |
Ishida, N.; Hirano, T.; Mukoyama, H. |
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Title |
Detection of aberrant alleles in the D-loop region of equine mitochondrial DNA by single-strand conformation polymorphism (SSCP) analysis |
Type |
Journal Article |
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Year |
1994 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
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25 |
Issue |
4 |
Pages |
287 |
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Keywords |
*Alleles; Animals; Base Sequence; *DNA, Mitochondrial; DNA, Single-Stranded/genetics; Female; Gene Frequency; Genomic Imprinting; Horses/*genetics; Male; Molecular Sequence Data; Pedigree; *Polymorphism, Genetic |
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Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo |
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0268-9146 |
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Notes |
PMID:7985852 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2213 |
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Permanent link to this record |
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Author |
Ishida, N.; Oyunsuren, T.; Mashima, S.; Mukoyama, H.; Saitou, N. |
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Title |
Mitochondrial DNA sequences of various species of the genus Equus with special reference to the phylogenetic relationship between Przewalskii's wild horse and domestic horse |
Type |
Journal Article |
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Year |
1995 |
Publication |
Journal of Molecular Evolution |
Abbreviated Journal |
J Mol Evol |
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Volume |
41 |
Issue |
2 |
Pages |
180-188 |
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Keywords |
Animals; Base Sequence; Chromosomes; Conserved Sequence/genetics; DNA, Mitochondrial/*genetics; Evolution; Genetic Variation/*genetics; Horses/*genetics; Molecular Sequence Data; *Phylogeny; RNA, Transfer, Pro/genetics; Sequence Alignment; Sequence Analysis, DNA |
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Abstract |
The noncoding region between tRNAPro and the large conserved sequence block is the most variable region in the mammalian mitochondrial DNA D-loop region. This variable region (ca. 270 bp) of four species of Equus, including Mongolian and Japanese native domestic horses as well as Przewalskii's (or Mongolian) wild horse, were sequenced. These data were compared with our recently published Thoroughbred horse mitochondrial DNA sequences. The evolutionary rate of this region among the four species of Equus was estimated to be 2-4 x 10(-8) per site per year. Phylogenetic trees of Equus species demonstrate that Przewalskii's wild horse is within the genetic variation among the domestic horse. This suggests that the chromosome number change (probably increase) of the Przewalskii's wild horse occurred rather recently. |
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Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo |
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0022-2844 |
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Notes |
PMID:7666447 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
5042 |
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Author |
Cottin, F.; Barrey, E.; Lopes, P.; Billat, V. |
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Title |
Effect of repeated exercise and recovery on heart rate variability in elite trotting horses during high intensity interval training |
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Journal Article |
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Year |
2006 |
Publication |
Equine Veterinary Journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
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Volume |
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Issue |
36 |
Pages |
204-209 |
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Keywords |
Animals; Electrocardiography/veterinary; Fatigue/diagnosis/*veterinary; Fourier Analysis; Heart Rate/*physiology; Horses/*physiology; Physical Conditioning, Animal/*methods/*physiology; Respiration; Time Factors |
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Abstract |
REASONS FOR PERFORMING STUDY: Interval training is a commonly used training method for trotting horses. In addition, trainers are provided with efficient and inexpensive heart rate monitor devices for the management of training. HYPOTHESIS: Since the high frequency (HF) frequency peak (fHF) of heart rate variability (HRV) corresponds to the breathing frequency in combination with stride frequency during trotting, it is hypothesised that modifications of breathing and stride frequencies induced by repeated exercise could be detected from fHF. METHODS: RR interval time series of 7 trotting horses were recorded during an interval training session. Interval training was made up of 5 successive 800 m high-velocity trotting runs (H1, H2...H5) separated by 1 min recovery bouts at low speed (R1, R2...R5). Fast Fourier transform (FFT) and Poincare plot analysis techniques were applied to RR series. RESULTS: Repeated exercise had significant effects on HRV components during interval training. Despite constant trotting velocities during high-speed and recovery, repetition induced a decrease in mean RR interval (H1: 295 +/- 19 vs. H5: 283 +/- 15 msec, P<0.05) and in the root mean square of successive differences in RR series (RMSSD; H1: 6.31 +/- 1.28 vs. H5: 5.31 +/- 1.31 msec, P<0.05). Furthermore, high-speed and recovery repetitions induced an increase in fHF (H1: 1.37 +/- 0.35 vs. H5: 1.62 +/- 0.40 Hz and R1: 0.22 +/- 0.02 vs. R4: 0.64 +/- 0.38 Hz, P<0.05). Hence, recovery induced a decrease in the s.d. of the successive RR series (SDRR; R3: 10.5 +/- 3.96 vs. R5: 6.17 +/- 2.65 msecs, P>0.05) and in the long term index of Poincare plot (SD2; R1: 43.29 +/- 28.90 vs. R5: 18.19 +/- 9.35 msecs, P<0.05). CONCLUSIONS: The observed increase in fHF during the interval training could be induced by alterations of the coupling between breathing and stride frequency linked to the emergence of fatigue. The decrease in SD2 and SDRR during successive recovery bouts could be linked with a deterioration of the recovery pattern. POTENTIAL RELEVANCE: HRV can provide breathing frequency data of Standardbreds during training without any respiratory device. Furthermore, HRV could provide useful makers of the emergence of fatigue states during training. |
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Laboratory of Exercise Physiology (LEPHE), University of Evry, E.A. 3872, Genopole, Boulevard F Mitterrand, F-91025 Evry cedex, France |
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Notes |
PMID:17402419 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
4009 |
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Permanent link to this record |
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Author |
Hagen, S.J.; Eaton, W.A. |
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Title |
Two-state expansion and collapse of a polypeptide |
Type |
Journal Article |
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Year |
2000 |
Publication |
Journal of Molecular Biology |
Abbreviated Journal |
J Mol Biol |
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Volume |
301 |
Issue |
4 |
Pages |
1019-1027 |
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Keywords |
Animals; Computer Simulation; Cytochrome c Group/*chemistry/*metabolism; Horses; Kinetics; Lasers; Models, Chemical; Peptides/*chemistry/*metabolism; Protein Conformation; Protein Denaturation; *Protein Folding; Spectrometry, Fluorescence; Temperature; Thermodynamics |
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Abstract |
The initial phase of folding for many proteins is presumed to be the collapse of the polypeptide chain from expanded to compact, but still denatured, conformations. Theory and simulations suggest that this collapse may be a two-state transition, characterized by barrier-crossing kinetics, while the collapse of homopolymers is continuous and multi-phasic. We have used a laser temperature-jump with fluorescence spectroscopy to measure the complete time-course of the collapse of denatured cytochrome c with nanosecond time resolution. We find the process to be exponential in time and thermally activated, with an apparent activation energy approximately 9 k(B)T (after correction for solvent viscosity). These results indicate that polypeptide collapse is kinetically a two-state transition. Because of the observed free energy barrier, the time scale of polypeptide collapse is dramatically slower than is predicted by Langevin models for homopolymer collapse. |
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Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Building 5, Bethesda, MD, 20892-0520, USA |
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0022-2836 |
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Notes |
PMID:10966803 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3790 |
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Permanent link to this record |
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Author |
Chiba, K.; Ikai, A.; Kawamura-Konishi, Y.; Kihara, H. |
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Title |
Kinetic study on myoglobin refolding monitored by five optical probe stopped-flow methods |
Type |
Journal Article |
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Year |
1994 |
Publication |
Proteins |
Abbreviated Journal |
Proteins |
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Volume |
19 |
Issue |
2 |
Pages |
110-119 |
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Keywords |
Animals; Chromatography, Gel; Circular Dichroism; Horses; Kinetics; Metmyoglobin/analogs & derivatives/chemistry; Myoglobin/*chemistry; *Protein Folding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Urea |
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Abstract |
The refolding kinetics of horse cyanometmyoglobin induced by concentration jump of urea was investigated by five optical probe stopped-flow methods: absorption at 422 nm, tryptophyl fluorescence at around 340 nm, circular dichroism (CD) at 222 nm, CD at 260 nm, and CD at 422 nm. In the refolding process, we detected three phases with rate constants of > 1 x 10(2) s-1, (4.5-9.3) s-1, and (2-5) x 10(-3) s-1. In the fastest phase, a substantial amount of secondary structure (approximately 40%) is formed within the dead time of the CD stopped-flow apparatus (10.7 ms). The kinetic intermediate populated in the fastest phase is shown to capture a hemindicyanide, suggesting that a “heme pocket precursor” recognized by hemindicyanide must be constructed within the dead time. In the middle phase, most of secondary and tertiary structures, especially around the captured hemindicyanide, have been constructed. In the slowest phase, we detected a minor structural rearrangement accompanying the ligand-exchange reaction in the fifth coordination of ferric iron. We present a possible model for the refolding process of myoglobin in the presence of the heme group. |
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Laboratory of Biodynamics, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa, Japan |
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ISSN |
0887-3585 |
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Notes |
PMID:8090705 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3799 |
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Permanent link to this record |
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Author |
Khalil, A.M.; Murakami, N.; Kaseda, Y. |
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Title |
Relationship between plasma testosterone concentrations and age, breeding season and harem size in Misaki feral horses |
Type |
Journal Article |
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Year |
1998 |
Publication |
The Journal of veterinary medical science / the Japanese Society of Veterinary Science |
Abbreviated Journal |
J Vet Med Sci |
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Volume |
60 |
Issue |
5 |
Pages |
643-645 |
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Keywords |
Age Factors; Animals; Animals, Wild; *Horses; Japan; Male; Reproduction; Seasons; Sexual Behavior, Animal; Sexual Maturation; *Social Behavior; Testosterone/*blood |
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Abstract |
Jugular vein blood samples were collected from 23 young and sexual mature feral stallions to examine the relationship between plasma testosterone concentration and age, breeding season or harem size. Testosterone concentration increased with the age of the stallions until they formed their own harems, at about 4 to 6 years old. Seasonal variations in testosterone concentrations were observed, and found to be significantly higher (P<0.001) throughout the breeding season than non-breeding season, from 3 years of age. Testosterone levels were correlated with harem size for individual stallions. It can be inferred from these results that there is a relationship between plasma testosterone concentration and age, breeding season and harem size. |
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Laboratory of Animal Behavioral Science, Faculty of Agriculture, Miyazaki University, Japan |
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ISSN |
0916-7250 |
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Notes |
PMID:9637303 |
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no |
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Call Number |
refbase @ user @ |
Serial |
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