Abstract: The reaction of Euglena gracilis cytochrome c-552 (cytochrome f) with the nonphysiological reactants potassium ferrocyanide, potassium ferricyanide, sodium ascorbate, sodium dithionite, and Chromatium vinosum high potential nonheme iron protein was studied by stopped-flow and temperature-jump kinetic methods. The reaction of the purified, water-soluble protein with the reactants was investigated as a function of ionic strength, pH, and temperature. The results demonstrated that reduction and oxidation takes place at a negatively charged site on the cytochrome c-552 surface. Participation of specific amino acid residues in electron transfer is implicated from the pH results. The results obtained for the nonphysiological reactions of cytochrome c-552 are compared with available data for horse heart cytochrome c and Rhodospirillum rubrum cytochrome c2. The results strongly suggest that Euglena gracilis cytochrome c-552 undergoes nonphysiological oxidation and reduction by a mechanism different from that found for cytochrome c or cytochrome c2.

Abstract: Reproductive performance was studied in 137 yearling mares run with stallions in small groups for 3 months between June and August in 1968 to 1971 (four breeding seasons). Pregnancy diagnosis by repeated rectal palpation and qualitative tests for PMSG, showed that ninety-five mares conceived of which forty-four aborted spontaneously between days 30 and 160 of gestation. Laboratory examination of twenty-one aborted fetuses failed to show any infectious agents. Serial quantitative and qualitative tests for PMSG in aborting animals gave results similar to those observed in mares with normal pregnancies. Plasma progestagen assays showed marked individual variations although the loss of the conceptus was always associated with a drop in progestagen level. The high incidence of early pregnancy loss in these pubertal mares may also be related to immaturity, inadequate nutrition and physical stress.

Abstract: In Experiment I, keylight was paired with inaccessible grain delivery (under two conditions of keylight intensity) to determine if autoshaping would occur in the absence of primary reinforcement. In Experiment II, the procedure was repeated with accessible grain, for comparison. In Experiment III, the procedures were repeated with explicitly unpaired presentations of keylight and either inaccessible or accessible grain. The results indicated that key pecking occurred as quickly in the presence of keylight pairings with inaccessible grain as with accessible grain, though (except for one bird) key pecking was not maintained with inaccessible grain. Furthermore, compared to the dim keylight, the bright keylight greatly suppressed key pecking when paired with inaccessible grain, and reduced the rate of key pecking when paired with accessible grain. Little key pecking occurred in groups exposed to explicitly unpaired presentations of keylight (whether bright or dim) and grain (whether accessible or inaccessible). When the birds in Experiment III were retested with explicitly paired presentations of keylight and grain, little key pecking was observed, suggesting suppressive effects of prior explicitly unpaired presentations. It is suggested that the effects of key-brightness manipulation were produced by the association of grain with cues other than the response key, or by distraction produced by partial illumination of the grain hopper.

Abstract: There are two distinct types of social organization and, accordingly, two types of mating systems in equids. In the horse, Plains zebra and Mountain zebra, the adults live in non-territorial and cohesive one-male groups and in stallion groups. The family stallions have exclusive mating rights which are respected by all others. In Grevy's zebra and in the African and Asiatic wild asses, the stallions are permanently territorial and have exclusive mating rights within their territories. Ecological and evolutionary aspects are discussed.