Abstract: Objective  The aims of this work were to (1) develop a low-cost equine movement tracking collar based on readily available components, (2) conduct preliminary studies assessing the effects of both paddock size and internal fence design on the movements of domestic horses, with and without foals at foot, and (3) describe distances moved by mares and their foals. Additional monitoring of free-ranging feral horses was conducted to allow preliminary comparisons with the movement of confined domestic horses. Procedures  A lightweight global positioning system (GPS) data logger modified from a personal/vehicle tracker and mounted on a collar was used to monitor the movement of domestic horses in a range of paddock sizes and internal fence designs for 6.5-day periods. Results  In the paddocks used (0.8-16 ha), groups of domestic horses exhibited a logarithmic response in mean daily distance travelled as a function of increasing paddock size, tending asymptotically towards approximately 7.5 km/day. The distance moved by newborn foals was similar to their dams, with total distance travelled also dependent on paddock size. Without altering available paddock area, paddock design, with the exception of a spiral design, did not significantly affect mean daily distance travelled. Feral horses (17.9 km/day) travelled substantially greater mean daily distances than domestic horses (7.2 km/day in 16-ha paddock), even when allowing for larger paddock size. Conclusions  Horses kept in stables or small yards and paddocks are quite sedentary in comparison with their feral relatives. For a given paddock area, most designs did not significantly affect mean daily distance travelled.

Abstract: Animals with eyes placed laterally on their head would respond differently to stimuli detected on their different sides. Visual cues from the left eye are processed in the right hemisphere which controls the intensive emotion expression and fast response to the stimuli, while the left hemisphere receiving the information from the contralateral eye is responsible for the routine behaviour and concentration. Different use of left/right eye for exploring environment and evaluation of different stimuli was recently described in domestic horses. They use left eye predominantly for watching novel object or frightening stimulus which indicates specialization of right hemisphere (RH) for appraisal of these types of stimuli. We presumed such lateralisation also in foals during suckling when only monocular vision can be employed for observing surroundings (foals suck mostly in antiparallel body position). Two mutually exclusive hypotheses about the origin of probable foal’s preference for particular suckling side were postulated: 1) visual lateralization, then the foals should suck more often from the mother’s right side (‘from right’), so as potential danger would be detected by the better adapted right hemisphere (i.e. left eye); 2) motor lateralization (the analogous concept as human “handness”), then a foal should suck predominantly from one side, either left or right, more often. Within two seasons, suckling behaviour of 59 Kladruby mares giving birth to 79 foals was observed from deliveries to abrupt weaning (4-7 months of age). From 10 607 recorded suckling solicitations, 50.2% were performed from right, thus there was no preference of the suckling side on a population level. However, we found large variability in the probability of suckling from right among individual foals (p<0.0001); the probability ranged from 0.22-0.98. One third of the foals (35.4%) showed strong, either right (N=13) or left (N=15) side preference which further strengthened with age (interaction suckling side preference*foal’s age: p<0.0001). Other tested variables, i.e. sex of the foal, age of the mother and identity of the herd were not significant. The probability that the mother rejected foal’s suckling solicitation (N=108) did not differ according to the suckling side (right/left) or suckling side preference (right/left/no preference). Suckling bout duration was not significantly influenced neither by the suckling side nor suckling side preference regardless the mother or the foal terminated the suckling bout. In conclusion, only 16 % of the foals revealed significant right side preference for suckling while 19 % left side and 65 % no preference. Thus, we can reject the visual hypothesis because a general trait to suck with left eye open for better danger detection and recognition is unlikely to occur, at least in up to 7 months old foals. Such a young foal is probably fully focused on suckling and relies on mother’s vigilance. Observed individual side preferences indicated more likely a kind of motor lateralization in part of the foals. Further research is needed to clear up the effects of age and individual experience of the foal. Supported by AWIN, EU FP7 project No. 266213.

Abstract: In a preliminary study, we evaluated the effect of precocious handling (at birth, 10, 30 and 60 days) and enriched environment on foal. Two experimental groups were created:
- 3 foals not handled and stabled in semi-enriched environment (Group A).
- 6 foals handled and stabled in an enriched environment (Group B).
All nine foals were tested with a novel object test (umbrella) at three months of age, to assess the Heart Rate (HR) values measured by a telemetric heart rate monitor (Polar Horse Trainer), during opening, touching and closing of the umbrella.
The statistical analysis carried out by means of the Mann-Whitney test showed lower HR values of the foals of group B (P<0.05).
Furthermore we have investigated 10 foals born between 2006 and 2008 to study the influence of enriched environment and precocious handling on behavioural responsiveness. The foals were housed on a farm which ensured an enriched environment and made social intra and interspecific interactions easier, alias autoshaping. They were submitted to:
- Handling within 24 hours from birth.
- Handling and reactivity test (arena test) executed at 10, 30 and 60 days.
The temporal correlation was studied among ages and baseline HR values, using the
Spearman"s test, to assess the beginning of neurovegetative HR control.
In fact statistically significant differences were obtained (r = -0.431; P<0,02). Temporal values of obtained HR baseline, using the Wilcoxon test, showed lower values at 60 days. The comparison was significant among mean and max baseline HR values of 30 vs 60 and 10 vs 60 (P<0.05), while no differences were observed during the reactivity test. This shows that adequate conditions of management associated to a precocious and continuous handling allow the foals to maintain their natural investigative behaviour disappearing fear and flight reactions.

Abstract: An early training procedure commonly termed “foal imprint training” is widely promoted in the horse industry. However, there have been no published scientific investigations of its efficacy. This study determined the effects of a training procedure on foals and their reaction to stimuli used in the early training procedure, and to a novel stimulus, at 1, 2 and 3 months of age. Twenty-five foals received a standard training procedure at 2, 12, 24, and 48 h after birth. After the training procedure, the foals received minimal additional handling that included veterinary treatments and occasional relocation. Twenty-two foals born over the same time period served as controls. All 47 (25 trained, 22 control) foals were tested at 1 month of age. Only 20 were available for testing at 2 months of age, and nine were available at 3 months. Percentage change from baseline heart rate, time required to complete exposure to each stimulus (foals that were more reactive took longer) and the behavior of each foal during the introduction of each stimulus were recorded. Overall, the control foals tended to receive lower (better) behavioral scores at 1 and 2 months of age. Foals that underwent the training procedure tended to require less time to complete exposure to the stimulus and had lower heart rates during exposure to the stimuli at 1 and 2 months of age. By 3 months of age, there were no significant differences between trained and control foals for any measures. Early training was not efficacious in this study.

Abstract: Some Thoroughbred stallion farms separate the mare from the foal during breeding, leaving the foal alone in its stall. Such separation often leads to distress in the foal, as evidenced by behavioural indicators such as vocalisations, pacing or moving about, pawing and striking the body against solid objects. This study examined whether the foal's distress is reduced if a handler accompanies it during the period of separation. A total of 57 foals on an Irish stud farm were observed: 27 foals were accompanied during separation and 30 were left in isolation but discretely observed from outside the stall. On average, unaccompanied foals vocalised at significantly (P<0.001) shorter intervals (14.8 s versus 26.8 s), and spent significantly (P<0.05) more time pawing (29.6 s versus 6.8 s) than accompanied ones. In addition, 17 of the unaccompanied foals hurled themselves against a solid object on at least one occasion, while not one of the accompanied foals did this (P<0.001). Age, gender and number of times the foal had been previously separated from the mare while she was being teased or covered were not significantly related to any of the behavioural indicators of distress. Overall, the data provide clear evidence that accompanying a foal reduces the distress that it exhibits when separated from the mare. This procedure has obvious welfare benefits in the short term, in that the foal both experiences less distress and has a reduced likelihood of injuring itself, and it may have longer-term benefits by reducing the chance of the animal later developing a locomotory stereotypical behaviour.