Abstract: REASONS FOR PERFORMING STUDY: While imprint training procedures have been promoted in popular magazines, they have received limited scientific investigation. OBJECTIVES: To determine the effects of a neonatal imprint training procedure on 6-month-old foals and to determine if any one session had a greater effect than others. METHODS: Foals (n = 131) were divided into the following treatments: no imprint training, imprint training at birth, 12, 24 and 48 h after birth or imprint training only at birth, 12, 24, 48, or 72 h after birth. Foals then received minimal human handling until they were tested at 6 months. RESULTS: During training, time to complete exposure to the stimulus was significant for only 2 of 6 stimuli. Percentage change in baseline heart rate was significant for only 2 of 10 stimuli. These 4 effects were randomly spread across treatments. CONCLUSIONS: Neither the number of imprint training sessions (0, 1, or 4) nor the timing of imprint training sessions (none, birth, 12, 24, 48, or 72 h after birth) influenced the foal's behaviour at 6 months of age. POTENTIAL CLINICAL RELEVANCE: In this study, imprint training did not result in better behaved, less reactive foals.