Abstract: The design of a captive environment should facilitate the expression of the natural behavioural repertoire of the species concerned. The domestic horse (Equus caballus) is a social, herd dwelling species that is often housed in isolation from con-specifics or kept in groups in paddock enclosures. Although the latter allows for social interaction and does not restrict movement per se, it may not promote natural activity patterns or group cohesion. The aim of the current study was to assess the impact of two different paddock designs on the behaviour of a stable group of horses (n=6: 2 mares, 4 geldings).
The paddock designs tested were adjacent and grassed similarly, but configured differently. A central paddock (75 x 75m) designated NT, was surrounded by a track 1.6-5.5m wide, designated T. The horses were turned out in their group into T or NT for 3 consecutive days. Their behaviour was recorded for one hour three times each day (10.00, 13.00, 16.00 hrs). Within each observation period of one hour focal sampling was used, each individual horse being observed for a 10 minute period. They were then moved to the other enclosure type for a further 3 consecutive days, followed by a repeat of each condition. When turned out the horses were fitted with a global positioning system device (Garmin Forerunner 305) to monitor distance travelled and speed for the period 10.00-17.00 hrs. The mean percentage of time spent in each behavioural state (standing alert, standing resting, walk/trot, grazing, lying, social interaction) during the periods observed was calculated. Behaviour during social interactions was classified as either affiliative (approach, follow, friendly contacts, mutual grooming) or agonistic (approach and retreat, bite, chase, head threat).
The horses travelled significantly further in T than in NT (paired samples t-test: t (5) = 11.74, p<0.001) and moved significantly faster (Wilcoxon signed rank test: z = -2.21, p=0.03). See Table 1. When the percentage of time spent in each behavioural state in T and NT was compared some significant differences were found. A significantly higher percentage of time was spent active (walking /trotting) in T than in NT (paired samples t-test: t (5) = 5.74, p=0.002). Standing alert was only recorded in T (paired samples t-test: t (5) = 3.48, p=0.02). A significantly higher percentage of time was spent grazing in NT than in T (paired samples t-test: t (5) = -3.58, p=0.016). Significantly more social interaction occurred in T than in NT (paired samples t-test: t (5) = 5.93, p=0.002). See Figure 1. In T, 91% of social interactions were affiliative and 9% agonistic, whereas in NT 29% were affiliative and 71% agonistic. No difference was found in the percentage of time spent standing resting or lying down in T and NT.
The benefits of housing horses in groups as opposed to individually have been demonstrated in previous studies. In addition to better satisfying the behavioural needs of the horse it has been found that group housed horses adapt more easily to training and display less undesirable behaviour than those housed individually (Rivera et al. 2002; Søndergaard and Ladewig 2004; Visser et al. 2008). However, individual housing is frequently selected by horse owners in preference to group housing to avoid the risk of injury during agonistic encounters. Fureix et al. (2012) suggest that management practices may well contribute to aggressiveness in horses and that the conditions under which we keep horses should be reviewed. The findings of the present study indicate that the design of the enclosure in which groups of horses are kept affects the nature of social interactions. The T paddock design resulted in reduced intra-group aggression. However, this paddock design also reduced the time spent grazing and increased vigilant behaviour. Although the results demonstrate that a paddock system including tracks may facilitate group cohesion and more natural movement patterns, the long-term impact on behaviour and welfare requires further investigation.
References:
Fureix C, Bourjade M, Henry S, Sankey C, Hausberger M. (2012). Exploring aggression regulation in managed groups of horses (Equus caballus). Applied Animal Behaviour Science 138: 216-228.
Rivera E, Benjamin S, Nielsen B, Shelle J, Zanella AJ. (2002). Behavioural and physiological response of horses to initial training: the comparison between pastured versus stalled horses. Applied Animal Behaviour Science 78: 235–252.
Søndergaard E, Ladewig J. (2004). Group housing exerts a positive effect on the behaviour of young horses during training. Applied Animal Behaviour Science 87: 105-118.

Abstract: For human athletes, any physical performance classically begins with a warm-up including a phase of cardio-respiratory activation as well as a phase of neuro-muscular mobilization. For the equine athletes similar routines are widely followed despite limited scientific studies addressing its effectiveness. Although physiological bases are of a similar nature in humans and horses, the latter are showing a different “telos” related to their status of prey. Indeed, their survival depends on their ability to detect any predator or potential danger in their environment and to estimate the need to run away. Therefore warm-up techniques should be adapted to this specificity and we added a walk phase preceding the usual warm-up during which the horse was allowed to visually assess its environment without constraint imposed by its rider, reins being long. We assumed that this walk phase would allow a more relaxed mental state and then a decrease in cognitive resources involved in environmental monitoring. Consequently this would release cognitive resources then available for the communication between the horse and the rider.
The autonomous nervous system (ANS) regulates the heart rate via the sympathetic and the parasympathetic nervous systems. The sympathovagal balance and the heart rate variability are considered as good indicators of an acute stress state as well as emotional states. These measures are frequently used to estimate well-being in animals. During the walk phase, we observed a significant decrease of the heart rate (HR), a significant increase of its variability and a significant modification of the sympathovagal balance in favour of a higher contribution of the parasympathetic control. We also measured the non-linear correlation dimension which reflects the degree of freedom of a system. To the best of our knowledge, this method was used until now only in humans to whom it was shown that its decrease is related to stressful events and is associated with a bad prognosis for survival. During the walk phase in horses, the dimension of correlation of cardiac activity was increased.
From a behavioural point of view, we observed a significant decrease of side movements related to head orientation as well as significant changes in ears position, the latter being preferentially directed forwards at the beginning of the walk phase and laterally at the end. As previous research suggests that ears position and head orientation can be involved in attentional mechanisms in horses, our results suggest a decrease of the attention focused on the environment. Furthermore, a significant lowering of the neck was observed as well as a significant decrease of behaviours related to stress.
To conclude our results suggest that a walk phase preceding the usual warm-up of horses would contribute to improve their mental state in favour of more relaxation and of a decrease of the attentional resources invested in environmental monitoring.

Abstract: This study replicated an unreported finding observed in a color perception experiment (Pick, Lovell, Brown, & Dail, 1994) where, after using the method of successive approximations to train a blue-gray discrimination, red-gray trials were initiated without further training. Although a gray choice had never been reinforced, the subject chose gray on the first 20 trials (p < .000001). In the study reported here, a horse was trained to approach a red feed bucket and not a green feed bucket. After the subject mastered the discrimination, a blue bucket was substituted for the previously reinforced red bucket. With double-blind controls in place, the subject chose the unreinforced green bucket on 15 out of the first 20 blue-green trials yielding a binomial p = 0.0148 that this outcome could be due to chance alone. These results are contrary to all behavioristic psychological learning theories, but consistent with prospect theory (Kahneman & Tversky, 1979). Prospect theory predicts that given a choice between two previously unreinforced stimuli, one familiar and the other novel, humans will choose the familiar. It is argued that the bias toward the familiar is the basis to a heuristic that has a genetic origin and should exist in other animals on the phylogenetic scale. The results of this study indicate that the heuristic is available at least as far down the scale as the horse. Conceptual replications using shape stimuli and sound stimuli are in progress.

Abstract: To assess horse welfare and develop a system for feedback to horse owners is the aim of the present study. A protocol developed in line with the Welfare Quality® project was used to assess 26 stables and 497 horses. Questionnaires were used to investigate what kind of feedback horse owners wanted and where they currently got their questions about horse welfare answered. The questionnaire was sent to participating stables and made available to the public via websites and social media.
Questionnaires revealed that 38% retrieved information from popular science articles, 77% from discussions with peers while 8% generally perceived their peers to lack knowledge on horse welfare. Factors affecting decision making were horse health (85%) and economy (38%). 85% wanted exhaustive information and advice on improvements, 69% preferred to get feedback as a digital document and 92% were interested in benchmarking.
Answers from participating stables lead to the development of a feedback consisting of results, scientific background of used measures, copies of assessment protocols for each horse, supportive telephone calls regarding decision making and benchmarking from all participating stables.
Questionnaires to the public had 688 respondents of which 54% were amateur riders/drivers. Main questions respondents had regarding horse welfare were within feeding regimes (62%), housing (57%) and field size (54%). Main motivational factors in decision making was horse health (83%) and behavioural problems (71%). 81% got information about horse welfare and support for changes from discussions with peers and 63% based decisions on their own personal opinion. 91% were interested in benchmarking scores to compare themselves with other stables.
The results highlight the need for independent assessment and feedback with a scientific base to horse owners. This will enable horse owners to make informed decisions with a scientific background that will result in increased horse welfare.
Lay persons message
A developed protocol was used to assess horse welfare and horse owners were questioned regarding how they wanted the results presented. This resulted in the development of a feedback system that will aid horse owners to make informed decisions about horse welfare.

Abstract: <p>Although previous studies of horses have investigated their facial expressions in specific contexts, e.g. pain, until now there has been no methodology available that documents all the possible facial movements of the horse and provides a way to record all potential facial configurations. This is essential for an objective description of horse facial expressions across a range of contexts that reflect different emotional states. Facial Action Coding Systems (FACS) provide a systematic methodology of identifying and coding facial expressions on the basis of underlying facial musculature and muscle movement. FACS are anatomically based and document all possible facial movements rather than a configuration of movements associated with a particular situation. Consequently, FACS can be applied as a tool for a wide range of research questions. We developed FACS for the domestic horse (<italic>Equus caballus</italic>) through anatomical investigation of the underlying musculature and subsequent analysis of naturally occurring behaviour captured on high quality video. Discrete facial movements were identified and described in terms of the underlying muscle contractions, in correspondence with previous FACS systems. The reliability of others to be able to learn this system (EquiFACS) and consistently code behavioural sequences was high?and this included people with no previous experience of horses. A wide range of facial movements were identified, including many that are also seen in primates and other domestic animals (dogs and cats). EquiFACS provides a method that can now be used to document the facial movements associated with different social contexts and thus to address questions relevant to understanding social cognition and comparative psychology, as well as informing current veterinary and animal welfare practices.</p>