Abstract: Chemical relaxation studies on the system horse liver alcohol dehydrogenase, nicotinamide adenine dinucleotide, and ethanol were conducted observing fluorescence changes between 400 and 500 nm. Temperature-jump experiments were performed at pH 6.5, 7.0, 8.0, and 9.0; concentration-jump experiments at pH 9.0. The reciprocal of the slowest relaxation time was found to be linearly dependent upon the enzyme concentration for relatively low enzyme concentrations, as predicted earlier. Use of the wide pH-range necessitated expression of the four apparent dissociation constants of the catalytic reaction cycle in terms of pH-independent constants. The system was described in terms of only one (or two) catalysis-linked protons not associated with the electron transfer. Protonic steps in a buffered system are in rapid equilibrium, too fast to be measured with the equipment available. Assuming only two of the four bimolecular reaction steps in the four-step cycle are fast compared to the remaining two, six cases may be considered with six expressions for the reciprocal of the slowest relaxation time. Comparison with the experimental data revealed that the bimolecular reaction steps governing the slowest relaxation time change with pH. Above the effective time resolution of the temperature-lump instrument with fluorescence detection (0.1 msec) only one other relaxation time was detectable and only at pH 9. This relaxation time, found to be independent of the concentration of all reactants within experimental error (r = 10 +/- 5 msec), is most likely due to an interconversion among ternary complexes.

Abstract: For red deer stags, fighting both has appreciable costs and yields considerable benefits. Up to 6% of rutting stags are permanently injured each year, while fighting success and reproductive success are closely related, within age groups as well as across them. Fighting behaviour is sensitive to changes in the potential benefits of fighting: stags fight most frequently and most intensely where potential benefits are high and tend to avoid fighting with individuals they are unlikely to beat. The relevance of these findings to theoretical models of fighting behaviour is discussed.

Abstract: Boer, Leobert E.M. de, and Jan & Inge Bouman, eds. Genetics and Hereditary Diseases of the Przewalski Horse. Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse 1979. 176p, several photos and charts. Reproduced from typescript, as issued. Very good paperbound (covers shelf-soiled). ** Publishes 18 papers given at the Arnhem Study-Conference of 1978, on many aspects of breeding Przewalski horses, their behavior, etc.