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Author Takaoka, A.; Maeda, T.; Hori, Y.; Fujita, K.
Title Do dogs follow behavioral cues from an unreliable human? Type Journal Article
Year 2015 Publication Animal Cognition Abbreviated Journal Anim.Cogn.
Volume 18 Issue 2 Pages 475-483
Keywords Dog; Dog–human interaction; Selective trust; Pointing; Object choice task
Abstract (up) Dogs are known to consistently follow human pointing gestures. In this study, we asked whether dogs “automatically” do this or whether they flexibly adjust their behavior depending upon the reliability of the pointer, demonstrated in an immediately preceding event. We tested pet dogs in a version of the object choice task in which a piece of food was hidden in one of the two containers. In Experiment 1, Phase 1, an experimenter pointed at the baited container; the second container was empty. In Phase 2, after showing the contents of both containers to the dogs, the experimenter pointed at the empty container. In Phase 3, the procedure was exactly as in Phase 1. We compared the dogs’ responses to the experimenter’s pointing gestures in Phases 1 and 3. Most dogs followed pointing in Phase 1, but many fewer did so in Phase 3. In Experiment 2, dogs followed a new experimenter’s pointing in Phase 3 following replication of procedures of Phases 1 and 2 in Experiment 1. This ruled out the possibility that dogs simply lost motivation to participate in the task in later phases. These results suggest that not only dogs are highly skilled at understanding human pointing gestures, but also they make inferences about the reliability of a human who presents cues and consequently modify their behavior flexibly depending on the inference.
Address
Corporate Author Thesis
Publisher Springer Berlin Heidelberg Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1435-9448 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5888
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Author Kaiser, S.; Hennessy, M.B.; Sachser, N.
Title Domestication affects the structure, development and stability of biobehavioural profiles Type Journal Article
Year 2015 Publication Frontiers in Zoology Abbreviated Journal
Volume 12 Issue 1 Pages 1-11
Keywords
Abstract (up) Domestication is an evolutionary process during which the biobehavioural profile (comprising e.g. social and emotional behaviour, cognitive abilities, as well as hormonal stress responses) is substantially reshaped. Using a comparative approach, and focusing mainly on the domestic and wild guinea pig, an established model system for the study of domestication, we review (a) how wild and domestic animals of the same species differ in behaviour, emotion, cognition, and hormonal stress responses, (b) during which phases of life differences in biobehavioural profiles emerge and (c) whether or not animal personalities exist in both the wild and domestic form. Concerning (a), typical changes with domestication include increased courtship, sociopositive and maternal behaviours as well as decreased aggression and attentive behaviour. In addition, domestic animals display more anxiety-like and less risk-taking and exploratory behaviour than the wild form and they show distinctly lower endocrine stress responsiveness. There are no indications, however, that domestic animals have diminished cognitive abilities relative to the wild form. The different biobehavioural profiles of the wild and domestic animals can be regarded as adaptations to the different environmental conditions under which they live, i.e., the natural habitat and artificial man-made housing conditions, respectively. Concerning (b), the comparison of infantile, adolescent and adult wild and domestic guinea pigs shows that the typical biobehavioural profile of the domestic form is already present during early phases of life, that is, during early adolescence and weaning. Thus, differences between the domestic and the wild form can be attributed to genetic alterations resulting from artificial selection, and likely to environmental influences during the pre- and perinatal phase. Interestingly, the frequency of play behaviour does not differ between the domestic and wild form early in life, but is significantly higher in domesticated guinea pigs at later ages. Concerning (c), there is some evidence that personalities occur in both wild and domestic animals. However, there may be differences in which behavioural domains – social and sexual behaviour, emotionality, stress-responsiveness – are consistent over time. These differences are probably due to changing selection pressures during domestication.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1742-9994 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Kaiser2015 Serial 5975
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Author Ahmadinejad , S.M.; Pishkar, J.; Anisi, T.; Babayee, B.
Title Verbal expressions of the horses to the pain Type Conference Article
Year 2015 Publication Proceedings of the 3. International Equine Science Meeting Abbreviated Journal Proc. 3. Int. Equine. Sci. Mtg
Volume Issue Pages
Keywords
Abstract (up) For communicating with the environment (other horses, foals, owner, etc.), horses have to use different methods. In contrast with the human, for whom talking is the most important way of communication, the horses can’t talk. In the years 1990s and before, the imagination was such that the infants do not express verbally to the pain either (like horses).

To communicate, horses use their body language. Vocalization (if not the body language), seems to be the most important way of communication in horses, though it seems they use the same tone when exposing to different events.

In this study which was performed in collaboration with the electronic institute of Sharif technical university (the top most technical university of the country), totally 25 horses were used. The horses were exposed to different events (hunger, pain, loneliness, mating, parturition and separation of the 1-2 weeks old foals from their dams). The verbal expressions of the horses were studied using spectrogram.

The results of this study showed that there were significant differences between the spectrograph of the voices of the horses, exposed to hunger, and the separation of the foals from dams. This was the same (no significant differences) when horses were exposed to loneliness, separation and hunger. There were no significant differences between the verbal expressions of the horses while exposed to mating, parturition.

One of the most important reasons why the horses do not have verbal expressions when exposing to the pain, might be the absence of the part of the brain, responsible for the pain interpretation. Morse research has to be performed to prove this.
Address
Corporate Author Ahmadinejad , S.M. Thesis
Publisher Xenophon Publishing Place of Publication Wald Editor Krueger. K.
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-3-95625-000-2 Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5890
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Author Jacquot, M.; Grosjean, A., Emrot, C.; Van-Erck-Westergem, E.; Schwartz, C.; Tomberg, C.
Title Effects of a walk phase at the warm up onset on physiological and behavioural parameters of ridden horses (Equus caballus) Type Conference Article
Year 2015 Publication Proceedings of the 3. International Equine Science Meeting Abbreviated Journal Proc. 3. Int. Equine. Sci. Mtg
Volume Issue Pages
Keywords
Abstract (up) For human athletes, any physical performance classically begins with a warm-up including a phase of cardio-respiratory activation as well as a phase of neuro-muscular mobilization. For the equine athletes similar routines are widely followed despite limited scientific studies addressing its effectiveness. Although physiological bases are of a similar nature in humans and horses, the latter are showing a different “telos” related to their status of prey. Indeed, their survival depends on their ability to detect any predator or potential danger in their environment and to estimate the need to run away. Therefore warm-up techniques should be adapted to this specificity and we added a walk phase preceding the usual warm-up during which the horse was allowed to visually assess its environment without constraint imposed by its rider, reins being long. We assumed that this walk phase would allow a more relaxed mental state and then a decrease in cognitive resources involved in environmental monitoring. Consequently this would release cognitive resources then available for the communication between the horse and the rider.

The autonomous nervous system (ANS) regulates the heart rate via the sympathetic and the parasympathetic nervous systems. The sympathovagal balance and the heart rate variability are considered as good indicators of an acute stress state as well as emotional states. These measures are frequently used to estimate well-being in animals. During the walk phase, we observed a significant decrease of the heart rate (HR), a significant increase of its variability and a significant modification of the sympathovagal balance in favour of a higher contribution of the parasympathetic control. We also measured the non-linear correlation dimension which reflects the degree of freedom of a system. To the best of our knowledge, this method was used until now only in humans to whom it was shown that its decrease is related to stressful events and is associated with a bad prognosis for survival. During the walk phase in horses, the dimension of correlation of cardiac activity was increased.

From a behavioural point of view, we observed a significant decrease of side movements related to head orientation as well as significant changes in ears position, the latter being preferentially directed forwards at the beginning of the walk phase and laterally at the end. As previous research suggests that ears position and head orientation can be involved in attentional mechanisms in horses, our results suggest a decrease of the attention focused on the environment. Furthermore, a significant lowering of the neck was observed as well as a significant decrease of behaviours related to stress.

To conclude our results suggest that a walk phase preceding the usual warm-up of horses would contribute to improve their mental state in favour of more relaxation and of a decrease of the attentional resources invested in environmental monitoring.
Address
Corporate Author Jacquot, M. Thesis
Publisher Xenophon Publishing Place of Publication Wald Editor ; Krueger, K.
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 978-3-95625-000-2 ISBN Medium
Area Expedition Conference
Notes Id - Approved no
Call Number Equine Behaviour @ team @ Serial 5897
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Author Bouskila, A.,; Lourie,E.; de Vries, H.; Hermans, Z.M .; van Dierendonck, M.
Title Sex, but not relatedness nor age, affect the social network of horses in a semi-natural reserve Type Conference Article
Year 2015 Publication Proceedings of the 3. International Equine Science Meeting Abbreviated Journal Proc. 3. Int. Equine. Sci. Mtg
Volume Issue Pages
Keywords
Abstract (up) For some time now, social network analysis provides tools to describe concisely the social structure of animals. Nevertheless, the factors that shape the social network and determine the frequency of different types of affiliations are often unclear. For example, the effects of relatedness on social networks have been only studied on relatively few species. Both among social or sub-social species the effects ranged from no effect to a substantial effect. Here we test the hypothesis that relatedness has an effect on the social network of horses (Equus caballus) that live freely in semi-natural conditions, and specifically, that individuals with high values of relatedness to their neighbors in the network will have fewer links Wolf et al, 2011). This hypothesis is based on the notion that related individuals have common interests and are likely to assist each other, and may need less links to other, unrelated individuals. In addition, individuals of similar age and sex are likely to have common needs, and thus are more likely to be associated. We thus tested a second hypothesis: homophily to individuals with similar age and sex will have a role in determining the associations within the social network. The field study was conducted on 27 horses in the Blauwe Kamer reserve in the Netherlands (1.1 sq km). We videotaped horses and their groups and used the information from 22 horses, after excluding the five foals from the analysis. Relatedness was calculated from the pedigree, which was based on parentage, determined by DNA analysis. The social network was constructed based on spatial proximity data. We assessed the influence of relatedness, age-homophily and sex-homophily on the network structure with Multiple Regression Quadratic Assignment Procedure (MRQAP) (Krackhardt 1988), with the R package sna. The results show that there was no significant effect of relatedness on the network, nor an effect of age-homophily. Nevertheless, we found a significant effect of sex-homophily, the tendency of individuals to associate with individuals of the same sex. We argue that the lack of a relatedness effect is not likely to have been caused due to the inability to detect who is kin. The structured social system in horses includes strong associations between often unrelated individuals, alongside with young individuals leaving their natal harem as part of the inherent inbreeding avoidance (Linklater & Cameron 2009; Boyd et al. in press). The significant effect of sex-homophily could stem from the protection females get from associations with other females, e.g., in the reduction of harassment from males. Previous studies on feral horses showed that mares that were better connected with other females in their harem benefited from higher survival rates to their foals (Cameron et al. 2009). The associations among bachelor males could also contribute to the strength of the sex-homophily effect.

In order to generalize from our results, one needs to examine additional populations of horses, because the conditions in the Blauwe Kamer reserve may not be representative, mainly due to the limited opportunity for dispersal in a restricted area.

Keywords: Long-term affiliation; spatial proximity, kin detection

Boyd, L., Scorolli, A. L., Nowzari, H., & Bouskila, A. (2016). Chapter 2: Social organization. In J. I. Ransom, & P. Kaczensky (Eds.), Wild equids. Baltimore, Maryland (in press): The Johns Hopkins University Press.

Cameron, E. Z., Setsaas, T. H., & Linklater, W. L. (2009). Social bonds between unrelated females increase reproductive success in feral horses. Proceedings of the National Academy of Sciences, 106(33), 13850-13853

Krackhardt, D. (1988). Predicting with networks: Nonparametric multiple regression analysis of dyadic data. Social Networks, 10(4), 359-381.

Linklater, W. L., & Cameron, E. Z. (2009). Social dispersal but with philopatry reveals incest avoidance in a polygynous ungulate. Animal Behaviour, 77(5), 1085-1093.

Wolf, J. B., Traulsen, A., & James, R. (2011). Exploring the link between genetic relatedness r and social contact structure k in animal social networks. The American Naturalist, 177(1), 135-142.
Address
Corporate Author Bouskila, A. Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5881
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Author Roubová, V.; Konecná, M.; Smilauer, P.; Wallner, B.
Title Whom to Groom and for What? Patterns of Grooming in Female Barbary Macaques (Macaca sylvanus) Type Journal Article
Year 2015 Publication Plos One Abbreviated Journal Plos One
Volume 10 Issue 2 Pages e0117298
Keywords
Abstract (up) Grooming is one of the most conspicuous social interactions among nonhuman primates. The selection of grooming partners can provide important clues about factors relevant for the distribution of grooming within a social group. We analyzed grooming behavior among 17 semi-free ranging female Barbary macaques (Macaca sylvanus). We tested whether grooming is related to kinship, rank and friendship. Furthermore, we tested whether grooming is reciprocated or exchanged for rank related benefits (i.e. lower aggression and increased tolerance whilst feeding). We found that in general grooming was reciprocally exchanged, directed up the hierarchy and at the same time affected by friendship and kinship. Grooming was more frequent among individuals with higher friendship values as well as amongst related individuals. We also divided our data set on the basis of rank difference and tested if different power asymmetries between individuals affected the tendency to exchange grooming for rank related benefits and grooming reciprocation. In support of our initial hypothesis our results show that the reciprocation of grooming was a significant predictor of grooming interactions between individuals of similar rank, but not between those individuals more distantly separated in the social hierarchy. However, we did not find any evidence for grooming being exchanged for rank related benefits in either data set. Our results, together with previously published studies, illustrate the behavioral flexibility of macaques. It is clear that multiple studies of the same species are necessary to gather the data required for the solid comparative studies needed to shed light on patterns of grooming behavior in primates.
Address
Corporate Author Thesis
Publisher Public Library of Science Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 6415
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Author Lovrovich, P.; Sighieri, C.; Baragli, P.
Title Following human-given cues or not? Horses (Equus caballus) get smarter and change strategy in a delayed three choice task Type Journal Article
Year 2015 Publication Applied Animal Behaviour Science Abbreviated Journal Appl. Anim. Behav. Sci.
Volume 166 Issue Pages 80-88
Keywords
Abstract (up) Highlights

�Horses remember the location of food hidden by the experimenter after a delay.

�They understand the communicative meaning of a human positioned close to the target.

�The same horses are capable of changing their decision-making strategy.

�They are able to shift from accuracy inferred from human given cues to speed.

�Horses can use human cues or not depending on time, cost, experience and reward.

Abstract

To date, horses have seemed capable of using human local enhancement cues only when the experimenter remains close to the reward, since they fail to understand the communicative meaning of the human as momentary local enhancement cue (when the human is not present at the moment of the animal's choice). This study was designed to analyse the ability of horses to understand, remember and use human-given cues in a delayed (10 s) three-choice task. Twelve horses (experimental group) had to find a piece of carrot hidden under one of three overturned buckets after seeing the experimenter hide it. The results were then compared with those of a control group (twelve horses) that had to find the carrot using only the sense of smell or random attempts. At the beginning, the experimental horses made more correct choices at the first attempt, although they took more time to find the carrot. Later the same horses were less accurate but found the carrot in less time. This suggests that the value of the proximal momentary local enhancement cues became less critical. It seemed, in fact, that the experimental and control group had aligned their behaviour as the trials proceeded. Despite this similarity, in the second half of the trials, the experimental group tended to first approach the bucket where they had found the carrot in the immediately preceding trial. Our findings indicate that horses are capable of remembering the location of food hidden by the experimenter after a delay, by using the human positioned close to the target as valuable information. The same horses are also capable of changing their decision-making strategy by shifting from the accuracy inferred from human given cues to speed. Therefore, horses are able to decide whether or not to use human given-cues, depending on a speed-accuracy trade-off.
Address
Corporate Author Thesis
Publisher Elsevier Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes doi: 10.1016/j.applanim.2015.02.017 Approved no
Call Number Equine Behaviour @ team @ Serial 5849
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Author König von Borstel, U.; Kienapfel, K.; McLean, A.; Wilkins, C.; Evans, D.; McGreevy, P.
Title Hyperflexing the horse‘s neck: a cost-benefit and meta-analysis Type Conference Article
Year 2015 Publication Proceedings of the 3. International Equine Science Meeting Abbreviated Journal Proc. 3. Int. Equine. Sci. Mtg
Volume Issue Pages
Keywords horse, head-and-neck posture, hyperflexion, welfare, gymnastics
Abstract (up) In ethical discussions, a cost-benefit analysis requires that welfare costs associated with an activity can be reliably estimated and balanced against the potential benefits of the activity to both humans and animals. The current study applies a meta-analysis to the peer-reviewed evidence for costs and benefits of hyperflexion of the neck in horses; a practice that has attracted enormous public and scientific scrutiny over the past 15 years. A literature review identified 55 studies dealing with horses’ head and neck postures. Fourty-two of these studies examined the impact of various postures on equine welfare, for example, by assessing behavior, physiological stress parameters, health or rider-horse interaction. Thirty-five studies examined the impact of various postures on gymnastics (e.g. kinematics, shifts in weight distribution, muscle activity, airway functioning or overall workload). For the meta-analysis a dataset containing information from each of the individual studies was created. Data included information such as type, degree, duration and circumstances of hyperflexion applied in that particular study as well as information on the horses (e.g., sport discipline, level of training, breed) and on the study design (e.g., size of study and experimental or epidemiological research design). The results of the study regarding the impact of hyperflexion on a) welfare and b) gymnastics were coded as positive (1), insignificant or contradictory (0) or negative (-1). The significant majority of studies (88%) concluded that a hyperflexed head and neck posture negatively impacts welfare. Just one study suggested welfare advantages of training in a hyperflexed head and neck posture. An analysis using a generalized linear mixed model to assess the influence of the above factors collated in the dataset revealed that none of these factors significantly influenced the probability of a study to detect negative welfare implications. Thus hyperflexing the neck appears to impair horses’ welfare regardless of, for example, the duration or the way of achieving hyperflexion. A concurrent assessment of the evidence for gymnastic benefits showed that approximately one quarter of studies conclude that there may be benefits, while another quarter of the studies conclude that hyperflexion has detrimental effects on gymnastics. Thus, on the costs-side there is a clear reduction in equine welfare and some undesirable gymnastic effects, as well as likely a compromised profile of the equestrian sports in public. Benefits, on the other hand, include some desirable gymnastic effects, and potentially increased control of the horse for the rider. On balance, it appears that the costs associated with hyperflexion exceed the potential benefits of the activity to both humans and horses.
Address
Corporate Author König von Borstel, U. Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5872
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Author Wolter, R.; Krueger, K.
Title The analysis of social bonds in feral horses Type Conference Article
Year 2015 Publication Proceedings of the 3. International Equine Science Meeting Abbreviated Journal Proc. 3. Int. Equine. Sci. Mtg
Volume Issue Pages
Keywords social bonds, grooming, nearest neighborhood, rank
Abstract (up) In many social mammals, individuals preferentially affiliate with a small subset of available partners instead of distributing their social behaviors equally among all group members. The resulting social bonds have been investigated in several mammalian taxa, especially in primate societies, but also in other taxa such as birds, dolphins, rodents and ungulates. In feral horses, a great number of studies on social bonds can be found, but with a huge variety between methods for the analysis. There seems to be a lack of a clear and common definition of social bonds in horses and of comparable analyses. For example, there are irregularities between the studies regarding the research designs, the selection of recording methods and the interpretation of the measurements. Mutual grooming is used most often for the analysis of social relationships in many species. As mutual grooming is rare in horses, especially measurements of spatial proximity are commonly used for the analysis of social bonds in addition to other behavioral patterns. However, the combination of mutual grooming and nearest neighborhood analyses for the analysis of social bond is debatable, as in contrast to mutual grooming, which must occur deliberately by both grooming partners, the spatial distribution can be influenced by one partner alone, which may even force the other horse to keep a certain distance or to stay in close proximity.

In this study, we investigated the comparability of mutual grooming and nearest neighborhood data for social bond analyses in feral horses. Therefore, we observed five groups of semi-wild living Przewalski’s horses and six groups of feral horses.

We analysed the horses’ social ranks by applying an Average Dominance Index, we recorded the distances between the animals and observed the number of mutual grooming events as well as friendly approaches.

Our results show that there was only a weak correlation between the frequency of staying in nearest neighborhood and mutual grooming in all observed horse groups. In contrast to this, the correlation between the number of friendly approaches and mutual grooming events was higher in most groups.

Hierarchies did not affect social bonds, as mutual grooming was similarly induced by higher and lower ranking animals and the social rank did not affect the choice of the grooming partner. Similarly, likelihoods of staying in the neighborhood of particular animals were not affected by the animals’ social rank.

The grooming frequencies differed between the different horse groups and between the individual horses living in the particular groups. They seem to be effected by individual predisposition.

Altogether we suggest that the ratio of mutual grooming seems to be a better indicator for social bonds in feral horses than the frequency of staying in the nearest neighborhood. Mutual grooming occurs deliberately and is bidirectional, whereas nearest neighborhoods could be enforced and unidirectional. For the calculation of social bonds in horses, we consider it to be more reliable to combine the frequency of mutual grooming with the frequency of friendly approaches.
Address
Corporate Author Wolter, R. Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-3-95625-000-2 Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5882
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Author Wyss, C.
Title Does housing in a „social box“ change faecal cortisol metabolites concentration in stallions? Type Conference Article
Year 2015 Publication Proceedings of the 3. International Equine Science Meeting Abbreviated Journal Proc. 3. Int. Equine. Sci. Mtg
Volume Issue Pages
Keywords housing system, stallions, social interaction, stress, faecal cortisol metabolites
Abstract (up) In order to improve the housing conditions of stallions in individual boxes by offering a possibility to have more social contact, the Swiss national stud farm tested a new box system for horses, allowing increased physical contact with the neighbouring stallion. The aim of this part of the study was to investigate whether this type of housing system (named “social box”) potentially induces a change in stress reactions in stallions compared to conventional boxes. Therefore faecal cortisol metabolite (FCM) concentration was measured as a non-invasive parameter to assess endocrine responses related to this new environment.

Four groups each consisting of eight adult Freiberger breeding stallions were included in the test design. Every stallion spent three weeks in a conventional box and in a social box respectively (cross-over design). The conventional box consisted of a separation wall with a lower opaque part and an upper part with vertical barriers (5 cm between barriers), allowing visual and olfactory contact but strongly limiting tactile contact. The separating wall of the social box consisted of two lateral sections, one part being opaque to the ceiling and the second part consisting of vertical barriers (30 cm between barriers), allowing the horse to have physical contact with its neighbour or to avoid it.

In horses, FCM concentration reflects an average level of circulating cortisol over a period of approximatively 24h. Faecal samples were collected the day following integration in social / conventional boxes, reflecting the potential stress induced by increased social interactions during the integration. In order to asses potential chronical stress, faeces samples were also collected in week one, two and three after the integration into the social / conventional box (in total: 4 samples per horse and housing system). The samples were immediately stored at -20°C until they were analysed. The samples were not analysed in the laboratory until the end of the experiment, therefore the duration of conservation in the freezer varied from 40 to 429 days.

A considerable percentage of data from groups 1 and 2 was below the detection limit (<0.8 ng/g) (Tab. 1). Thus the statistical analysis was conducted with the FCM concentration from groups 3 and 4 (n horses = 16) which contained no values below the detection limit.

Tab. 1: Details about FCM values and storage time for the 4 groups of stallions

Group Storage duration [d] Proportion of data below the detection limit (<0.8 ng/g) Mean [ng/g] Median [ng/g]

Group 1 384-429 55.6 % 2.2 0

Group 2 315-360 25.5 % 5.8 6.3

Group 3 41-79 0.0 % 8.7 8.0

Group 4 40-85 0.0 % 5.8 5.4

Despite the impressive social interactions observed between the stallions directly after being introduced into the social boxes, we did not find any differences in FCM concentration between the stallions being introduced into the conventional box and the social box on the day of integration (social box: n samples = 16, mean±SD: 6.9±4.7 ng/g; conventional box: n samples = 16, mean±SD: 9.0±11.2 ng/g; Wilcoxon signed rank test V = 70, p = 0.94).

Overall the samples taken during integration and in week one, two and three did not show evidence of changes in FCM concentration in either housing system over a longer period of time (social box: n samples = 64, mean±SD: 7.9±6.2 ng/g; conventional box: n samples = 64, mean±SD: 6.6±3.4 ng/g; Linear mixed model (LMM), p = 0.56).

Our results suggest that the possibility of having physical contact with a conspecific does not induce changes in FCM concentration in breeding stallions. The considerable percentage of values below the detection limit in groups 1 and 2 seemed to correlate with the increasing duration of storage before analysis. During the IESM Network Meeting 2015, we would like to discuss possible methodological issues and the possibilities to correctly integrate these low values in the statistical analysis.
Address
Corporate Author Wyss, C. Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5869
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