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Author Touma, C.; Sachser, N.; Mostl, E.; Palme, R.
Title Effects of sex and time of day on metabolism and excretion of corticosterone in urine and feces of mice Type Journal Article
Year 2003 Publication General and Comparative Endocrinology Abbreviated Journal Gen Comp Endocrinol
Volume 130 Issue 3 Pages 267-278
Keywords Animals; Chromatography, High Pressure Liquid; Circadian Rhythm/*physiology; Corticosterone/*metabolism/urine; Feces/*chemistry; Female; Immunoenzyme Techniques; Kinetics; Male; Mice; Mice, Inbred C57BL; Reference Values; Sex Factors; Stress/metabolism; Time Factors; Tritium
Abstract Non-invasive techniques to monitor stress hormones in small animals like mice offer several advantages and are highly demanded in laboratory as well as in field research. Since knowledge about the species-specific metabolism and excretion of glucocorticoids is essential to develop such a technique, we conducted radiometabolism studies in mice (Mus musculus f. domesticus, strain C57BL/6J). Each mouse was injected intraperitoneally with 740 kBq of 3H-labelled corticosterone and all voided urine and fecal samples were collected for five days. In a first experiment 16 animals (eight of each sex) received the injection at 9 a.m., while eight mice (four of each sex) were injected at 9 p.m. in a second experiment. In both experiments radioactive metabolites were recovered predominantly in the feces, although males excreted significantly higher proportions via the feces (about 73%) than females (about 53%). Peak radioactivity in the urine was detected within about 2h after injection, while in the feces peak concentrations were observed later (depending on the time of injection: about 10h postinjection in experiment 1 and about 4h postinjection in experiment 2, thus proving an effect of the time of day). The number and relative abundance of fecal [3H]corticosterone metabolites was determined by high performance liquid chromatography (HPLC). The HPLC separations revealed that corticosterone was extensively metabolized mainly to more polar substances. Regarding the types of metabolites formed, significant differences were found between males and females, but not between the experiments. Additionally, the immunoreactivity of these metabolites was assessed by screening the HPLC fractions with four enzyme immunoassays (EIA). However, only a newly established EIA for 5alpha-pregnane-3beta,11beta,21-triol-20-one (measuring corticosterone metabolites with a 5alpha-3beta,11beta-diol structure) detected several peaks of radioactive metabolites with high intensity in both sexes, while the other EIAs showed only minor immunoreactivity. Thus, our study for the first time provides substantial information about metabolism and excretion of corticosterone in urine and feces of mice and is the first demonstrating a significant impact of the animals' sex and the time of day. Based on these data it should be possible to monitor adrenocortical activity non-invasively in this species by measuring fecal corticosterone metabolites with the newly developed EIA. Since mice are extensively used in research world-wide, this could open new perspectives in various fields from ecology to behavioral endocrinology.
Address Department of Behavioral Biology, Institute of Neuro and Behavioral Biology, University of Muenster, Badestrasse 9, D-48149 Muenster, Germany. touma@uni-muenster.de
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title (up) Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0016-6480 ISBN Medium
Area Expedition Conference
Notes PMID:12606269 Approved no
Call Number Equine Behaviour @ team @ Serial 4086
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Author Sighieri, C.; Tedeschi, D.; De Andreis, C.; Petri, L.; Baragli, P.
Title Behaviour patterns of horses can be used to establish a dominantsubordinate relationship between man and horse Type Journal Article
Year 2003 Publication Animal Welfare Abbreviated Journal
Volume 12 Issue Pages 705-708
Keywords ANIMAL WELFARE; BEHAVIOUR PATTERNS; DOMINANCE; UNHANDLED HORSE
Abstract This paper describes how man can enter the social hierarchy of the horse by mimicking the behaviour and stance it uses to establish dominance. A herd is organised according to a dominance hierarchy established by means of ritualised conflict. Dominance relationships are formed through these confrontations: one horse gains the dominant role and others identify themselves as subordinates. This study was conducted using five females of the Haflinger breed, totally unaccustomed to human contact, from a free-range breeding farm. The study methods were based on the three elements fundamental to the equilibrium of the herd: flight, herd instinct and hierarchy. The trainer-horse relationship was established in three phases: retreat, approach and association. At the end of the training sessions, all of the horses were able to respond correctly to the trainer. These observations suggest that it is possible to manage unhandled horses without coercion by mimicking their behaviour patterns.
Address
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Publisher Place of Publication Editor
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Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4089
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Author de Waal, F. B.; Tyack, P., (eds)
Title Animal Social Complexity: Intelligence, Culture, and Individualized Societies Type Book Whole
Year 2003 Publication Abbreviated Journal
Volume Issue Pages
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Address
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Publisher Harvard University Press Place of Publication Cambridge, Massachusetts Editor de Waal, F. B.; Tyack, P.,
Language Summary Language Original Title
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ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4096
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Author Waring, G.H.
Title Horse behavior Type Book Whole
Year 2003 Publication Abbreviated Journal
Volume Issue Pages
Keywords
Abstract Review

The coverage in the book is incredibly broad, thorough and detailed. The drawings throughout are marvelous and do a wonderful job of complementing the text. The extensive bibliography should be especially useful to biologists. “Cheryl Asa, Director of Research, St. Louis Zoo”

For those highly interested in the subject, it is unquestionably worth upgrading rrom the first edition. Written by an internationally know and respected ethologist, Horse Behavior is a sound scientific review of equine behavior.

“Nancy Kate Diehl, ”Journal of American Veterinary Medical Association,“ Vol. 223, No. 12, December 15, 2003

One of the people out there studying horses is Dr. Waring, a professor at Southern Illinois University. He uses big words such as polyphasic, but defines them, ”The daily sleep cycle of horses is polyphasic, that is, with more than one period of sleep occurring per 24-hour period.“ He gives the academically correct citations of studies, yet describes the results in language the rest of us can understand. I dare any horseperson of any experience level to read it and not learn something.

”Katherine Walcott, Eventing USA, Issue Two, 2003“

Horse Behavior, 2nd edition, is a very well researched book that addresses a through review of normal horse behavior. Dr. Waring brings together references from a wide variety of disciplines, often from very difficult to get sources, and integrates them with his own research into a comprehensive picture of the horse. Each chapter is well orgainzed in its contents, resulting in a book that will be an excellent reference. This is a ”must have“ for any serious student of horse behavior and for those who just enjoy the animal. ”Bonnie V. Beaver, BS, DVM, MS, DACVB, Texas A&M University"

Book Description

The second edition of this important reference provides important updates, especially in the areas of activity patterns, social behavior, reproduction, animal husbandry, and management. This easy-to-read text integrates findings from hundreds of international researchers and includes an updated appendix listing behavioral symptoms and possible causes. Over 100 illustrations and photos provide excellent visual cues for those who work with horses every day.
Address
Corporate Author Thesis
Publisher Noyes Publishing Place of Publication Norwich, N.Y. Editor
Language Summary Language Original Title
Series Editor Series Title (up) Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 0815514840 9780815514848 Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ 48773749 Serial 4275
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Author Broucek, J.; Ksac, P.; Uhrincat, M.
Title The effect of sire line on learning and locomotor behaviour of heifers Type Journal Article
Year 2003 Publication Czech Journal of Animal Science Abbreviated Journal Czech J. Anim. Sci
Volume 48 Issue Pages 387-394
Keywords heifers; sire; maze; open-field test; repeatability; learning; locomotor behaviour relationship
Abstract ABSTRACT: e aim of this study was to test the effect of sire line on maze learning ability and locomotor behaviour

in open-field tests of heifers, consistency over the time of grid crossing and relationship between the time of traversing the maze and grid crossings in open-field tests, respectively. We analysed the results of ethological tests for 54 Holstein heifers that descended from 7 sires. Maze behaviour was observed at the age of 15 weeks, an open-field test was applied at two age periods, 16 weeks and 18 months. We found out highly significant differences in the time of traversing the maze between heifers of different sire origin (P < 0.01). &#57426;e number of grid crossings over the five minutes of the open-field test did not differ between the daughters of the age of 16 weeks and 18 months. Repeatability between the number of grid crossings at the age of 16 weeks and 18 months was proved by significant correlation (r = 0.2713*). On the contrary, significant relationships between the times of traversing the maze and locomotor behaviour in the open-field test (r =-0.3739*) were found only when the sequence of observations followed

after a week pause (age of 15 and 16 weeks).
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Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4322
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Author Russell, L.A.
Title Decoding Equine Emotions Type Journal Article
Year 2003 Publication Society and Animals Abbreviated Journal
Volume 11 Issue 3 Pages 265-266
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Address
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Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4383
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Author Fazio, F.; Assenza, A.; Piccione, G.; Caola, G.
Title Periodic Monitoring of Some Physiological Parameters during Training in the Athletic Horse Type Journal Article
Year 2003 Publication Veterinary Research Communications Abbreviated Journal
Volume 27 Issue Pages 595-598
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Address
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Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4384
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Author Wilson, R.T.
Title Biodiversity of Domestic Livestock in the Republic of Yemen Type Journal Article
Year 2003 Publication Tropical Animal Health and Production Abbreviated Journal
Volume 35 Issue 1 Pages 27-46
Keywords Abstract This paper describes the domestic livestock of the Republic of Yemen and aspires to complement earlier sources listing or partially describing `breeds'. It attempts to cover all species and provide indications of production parameters through a literature review and via field observations made by the author in 1999. Information is provided on livestock numbers and the economic importance of animal production. Most animals are kept in sedentary mixed crop-livestock production systems; transhumant systems have the next greatest number of stock; with nomadic systems being of least and declining importance. Yemen's livestock appear to comprise at least 11 breeds of sheep, 5 breeds of goat, 2 breeds of cattle, 4 breeds of camel, 2 breeds of donkey and 1 breed of horse. There are no data on breeds of poultry but domestic fowl (where clearly considerable diversity exists) and pigeons are kept. There is little formal information on the history and relationships of most breeds. Some appear to be of ancient local origin, whereas others show affinities with those of neighbouring and other countries. None of the identified types is considered endangered, so conservation would be premature. A more formal and detailed genetic characterization, to add to the largely morphological and traditional classification, may, however, reveal such a need.
Abstract Abstract This paper describes the domestic livestock of the Republic of Yemen and aspires to complement earlier sources listing or partially describing `breeds'. It attempts to cover all species and provide indications of production parameters through a literature review and via field observations made by the author in 1999. Information is provided on livestock numbers and the economic importance of animal production. Most animals are kept in sedentary mixed crop-livestock production systems; transhumant systems have the next greatest number of stock; with nomadic systems being of least and declining importance. Yemen's livestock appear to comprise at least 11 breeds of sheep, 5 breeds of goat, 2 breeds of cattle, 4 breeds of camel, 2 breeds of donkey and 1 breed of horse. There are no data on breeds of poultry but domestic fowl (where clearly considerable diversity exists) and pigeons are kept. There is little formal information on the history and relationships of most breeds. Some appear to be of ancient local origin, whereas others show affinities with those of neighbouring and other countries. None of the identified types is considered endangered, so conservation would be premature. A more formal and detailed genetic characterization, to add to the largely morphological and traditional classification, may, however, reveal such a need.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title (up) Abbreviated Series Title
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ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4389
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Author Fazio, E.; Ferlazzo, A.
Title Evaluation of Stress During Transport Type Journal Article
Year 2003 Publication Veterinary Research Communications Abbreviated Journal
Volume 27 Issue Pages 519-524
Keywords hormones – physiological variables – stress – transport procedures
Abstract Domestic animals are transported for a variety of reasons including breeding, biomedical purposes, slaughter and, in the case of sporting horses, for competitions, pleasure activities or ceremonial proceedings. Studies to determine the amount of stress on farm animals during transport often have highly variable results and are difficult to interpret. The reaction of animals to stressors depends on the duration and intensity of the stressors, the animal's previous experience, its physiological status and the immediate environmental restraints. Behavioural, haematological, haematochemical, physiological and neuro-hormonal (ß-endorphin, ACTH, cortisol, iodothyronines) variables are discussed on the basis of handling, loading and transport procedures of animals.
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Publisher Place of Publication Editor
Language Summary Language Original Title
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Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4391
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Author Hedman, J.
Title Heart rate response towards fear-eliciting stimuli in horses Type Manuscript
Year 2003 Publication Sveriges lantbruksuniversitetSveriges lantbruksuniversitet Veterinärprogrammet Abbreviated Journal
Volume 2004 Issue 40 Pages
Keywords horse, fear, heart-rate, novel stimuli
Abstract Finding the right horse for each rider is a difficult task as it is just as important that the temperament of the horse fits the rider as it is that the horse is of the right size. Even though it is a commonly experienced problem, no objective method of easily measuring the horse“s temperament has yet been developed. If it is possible to test horses and get an objective measure of how reactive (emotional) they are, it could be a big help in finding the right horse for each rider. It would be desirable to have a way of testing the horse”s reaction in an unfamiliar (and potentially frightening) situation. In practice this test should be just as easy as it is getting a judgement of its conformation and gaits.

The aim of the present study was to measure individual variation in HR response to different novel objects in horses of the same age, breed and reared in the same environment. We wanted to see whether certain horses (i.e. more emotional horses) react more to novel stimuli, in general, than other horses (i.e. less emotional), irrespective of the type of stimulus. We also wanted to see if different novel stimuli elicited different responses within individuals. The hypothesis was that individuals will react in a similar way to various stimuli.

Twenty four Danish warmblood horses were included in this study. All horses were 2 year-old stallions, reared under similar environmental conditions. They had received a minimum of handling prior to the experimental period. Three different stimuli were used. They were chosen because they were novel to the horses and would elicit measurable fear-reactions in all horses, but not so much that the horses did not approach the feed within the duration of the test. The visual stimulus consisted of a 1meter high orange traffic cone with reflex stripes, placed 1 m in front of the tub, the olfactory stimulus was eucalyptus oil and the auditory stimulus was a radio tuned to white noise. The control was an empty arena.

The result was that only the HR response to the auditory and visual stimuli differed significantly from the control days. The olfactory stimulus did not seem to alarm the horses the way the other stimuli did. We found a tendency towards a correlation in reaction between the olfactory and auditory stimuli and between the auditory and visual stimuli within individuals. These results indicate that horses do not generalize completely in their reaction between different stimuli.
Address
Corporate Author Thesis Bachelor's thesis
Publisher Place of Publication Dept. of Animal Environment and Health, SLU. Examensarbete / Sveriges lantbruksuniversitet, Veterinä Editor
Language Summary Language Original Title
Series Editor Series Title (up) Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1650-7045 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4652
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