| Records |
| Author |
Gerber, V.; Robinson, N.E.; Luethi, S.; Marti, E.; Wampfler, B.; Straub, R. |
| Title |
Airway inflammation and mucus in two age groups of asymptomatic well-performing sport horses |
Type |
Journal Article |
| Year |
2003 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
35 |
Issue |
5 |
Pages |
491-495 |
| Keywords |
Age Factors; Animals; Bronchoalveolar Lavage Fluid/*cytology; Female; Flow Cytometry/veterinary; Horse Diseases/*physiopathology; Horses; Housing, Animal; Inflammation/veterinary; Leukocyte Count/veterinary; Male; Mucus/*secretion; Physical Conditioning, Animal; Respiratory Function Tests/veterinary; Respiratory Tract Diseases/physiopathology/*veterinary; Sports |
| Abstract |
REASONS FOR PERFORMING STUDY: Horses housed in conventional stable environments are exposed to high dust loads. Long-term exposure to inhaled dust may lead to subclinical airway disease. HYPOTHESES: Airway inflammation and mucus accumulation are 1) common in asymptomatic well-performing sport horses, 2) increased in older compared to younger individuals and 3) correlated. METHODS: Mucus quantity and quality (endoscopic scoring) and BALF differential cytology were assessed in 13 younger horses (mean age 5 years) and 13 older horses (mean age 15 years), which had no historical or clinical evidence of lower airway disease, were performing well and were housed permanently in a conventional stable environment. RESULTS: 1) Airway inflammation and/or mucus accumulation were very common. All sport horses showed evidence of subclinical inflammatory airway disease (IAD). 2) Older horses, having been exposed for 10 more years to a conventional stable environment, did not show increased subclinical airway inflammation or mucus accumulation. The only differences between the age groups were increased BALF total and lymphocyte cell counts in the younger horses. 3) Mucus quantity and quality scores were not significantly correlated with each other or with BALF neutrophil percentages and absolute numbers. CONCLUSIONS: Although clinically healthy and performing well, all of the examined horses housed in a conventional stable environment showed evidence of IAD. The degree of IAD was not increased in older horses that had not developed clinical signs. POTENTIAL RELEVANCE: The effect of subclinical degrees of IAD on performance in dressage and show jumping should be investigated further to better understand the clinical significance of IAD in sport horses. |
| Address |
Pulmonary Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, USA |
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| Language |
English |
Summary Language |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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Expedition |
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Conference |
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| Notes |
PMID:12875328 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3721 |
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| Author |
Dyson, S.; Murray, R. |
| Title |
Pain associated with the sacroiliac joint region: a clinical study of 74 horses |
Type |
Journal Article |
| Year |
2003 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
35 |
Issue |
3 |
Pages |
240-245 |
| Keywords |
Age Factors; Analgesia/veterinary; Anesthetics, Local/pharmacology; Animals; Body Height; Body Weight; Breeding; Female; Forelimb; Gait; Hindlimb; Horse Diseases/*diagnosis/radionuclide imaging; Horses; Lameness, Animal/*physiopathology; Lumbar Vertebrae/physiopathology; Male; Pain/diagnosis/drug therapy/radionuclide imaging/*veterinary; Sacroiliac Joint/*physiopathology; Sacrum/physiopathology |
| Abstract |
REASONS FOR PERFORMING STUDY: There has been no large study of horses with suspected sacroiliac (SI) joint region pain in which the clinical diagnosis has been supported by either abnormal radiopharmaceutical activity in the SI joint region or by periarticular infiltration of local anaesthetic solution. OBJECTIVES: To describe the clinical features of horses with SI joint region pain, to document the age, breed, sex, discipline, size and conformation of affected horses and to compare these with the author's (SD) normal case population and to document the results of infiltration of local anaesthetic solution around the SI joint region. METHODS: Horses were selected for inclusion in the study based upon the exclusion of other causes of lameness or poor performance, together with clinical signs suggestive of SI joint pain and abnormal radiopharmaceutical activity in the SI joint region and/or a positive response to periarticular infiltration of local anaesthetic solution. RESULTS: Sacroiliac joint region disease was identified in 74 horses between November 1997 and March 2002. Dressage and showjumping horses appeared to be at particular risk (P < 0.001). Affected horses were generally slightly older than the normal clinic population (P < 0.0001), taller at the withers (P < 0.0001) and of greater bodyweight (P < 0.01). There was a significant effect of breed (P < 0.001), with a substantially higher proportion of Warmblood horses (51%) in the SI pain group compared to the normal clinic population (29%). There was no correlation between conformation and the presence of SI joint region pain. The tubera sacrale appeared grossly symmetrical in most (95%) horses. Poor development of the epaxial muscles in the thoracolumbar region and asymmetry of the hindquarter musculature were common. Twenty-six horses (35%) showed restricted flexibility of the thoracolumbar region and 10 (16%) had an exaggerated response to pressure applied over the tubera sacrale. Fourteen horses (19%) were reluctant to stand on one hindlimb for prolonged periods. The majority of horses (75%) had a straight hindlimb flight and only 18% moved closely behind or plaited. In all horses restricted hindlimb impulsion was the predominant feature; invariably this was most obvious when the horse was ridden. Stiffness, unwillingness to work on the bit and poor quality canter were common. Sacroiliac joint region pain was seen alone (47%), or in conjunction with thoracolumbar pain (16%), hindlimb lameness (20%), forelimb lameness (7%) or a combination of problems (10%). Seventy-three horses (99%) had abnormalities of the SI joint region identified using nuclear scintigraphy. Infiltration of local anaesthetic solution around the SI joint region produced profound improvement in gait in all 34 horses in which it was performed. CONCLUSIONS AND POTENTIAL RELEVANCE: Careful clinical examination combined with scintigraphic evaluation of the SI joint region and local analgesia can enable a more definitive diagnosis of SI joint region pain than has previously been possible. |
| Address |
Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK |
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English |
Summary Language |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:12755425 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3723 |
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| Author |
Herholz, C.P.; Gerber, V.; Tschudi, P.; Straub, R.; Imhof, A.; Busato, A. |
| Title |
Use of volumetric capnography to identify pulmonary dysfunction in horses with and without clinically apparent recurrent airway obstruction |
Type |
Journal Article |
| Year |
2003 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
| Volume |
64 |
Issue |
3 |
Pages |
338-345 |
| Keywords |
Aging; Airway Obstruction/*complications/diagnosis/*veterinary; Animals; Capnography/*methods/*veterinary; Female; Horse Diseases/*diagnosis; Horses; Lung Diseases/complications/*diagnosis/*veterinary; Male; Predictive Value of Tests |
| Abstract |
OBJECTIVE: To investigate whether volumetric capnography indices could be used to differentiate between horses without recurrent airway obstruction (RAO) and horses with RAO that were in clinical remission or that had clinically apparent RAO. ANIMALS: 70 adult Swiss Warmblood horses (20 used for pleasure riding and 50 used for dressage or show jumping). PROCEDURE: Horses were allocated to 4 groups on the basis of history, clinical signs, results of endoscopy, and cytologic findings (group 1, 21 healthy horses; group 2, 22 horses with RAO that were in remission; group 3, 16 horses with mild RAO; group 4, 11 horses with exacerbated RAO). Expiratory volume and CO2 curves were recorded by use of a computerized ultrasonic spirometer. Volumetric capnograms were plotted, and derived indices were calculated. RESULTS: Dead-space volume (VD) was calculated by use of the Bohr equation (VD(Bohr)) and for physiologic VD (VD(phys)). Ratios for VD(Bohr) to expiratory tidal volume (VT) and VD(phys) to V(T) as well as an index of effective CO2 elimination were significantly different among groups of horses. Age and use of the horses also significantly affected volumetric capnography indices. CONCLUSIONS AND CLINICAL RELEVANCE: Ratios of VD(Bohr) to VT and VD(phys) to VT as well as an index of effective CO2 elimination were sufficiently sensitive measures to distinguish between healthy horses and horses with RAO in remission. To optimize the ability of volumetric capnography indices to differentiate among horses in heterogeneous populations, it is important to account for effects of age and specific use of the horses. |
| Address |
Department of Equine Internal Medicine, Faculty of Veterinary Medicine, University of Berne, Langgasstr 124, CH-3012 Berne, Switzerland |
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English |
Summary Language |
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Series Issue |
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Edition |
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| ISSN |
0002-9645 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:12661875 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3724 |
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| Author |
Herholz, C.; Straub, R.; Braendlin, C.; Imhof, A.; Luthi, S.; Busato, A. |
| Title |
Measurement of tidal breathing flow-volume loop indices in horses used for different sporting purposes with and without recurrent airway obstruction |
Type |
Journal Article |
| Year |
2003 |
Publication |
The Veterinary Record |
Abbreviated Journal |
Vet. Rec. |
| Volume |
152 |
Issue |
10 |
Pages |
288-292 |
| Keywords |
Airway Obstruction/*physiopathology; Animals; Horses/*physiology; Physical Conditioning, Animal/*physiology; *Respiration; Respiratory Function Tests/*veterinary; Sports; Tidal Volume/physiology |
| Abstract |
Tidal breathing flow-volume loop (TBFVL) indices in healthy horses and in horses suffering from mild and moderate to severe recurrent airway obstruction (RAO) were measured. In addition, the ability of these indices to differentiate healthy controls from horses with mild RAO by separating the horses according to their usage was investigated. The horses were assigned to three sporting categories: 1 Leisure horses; 2 Horses competing at events with a duration of more than five minutes (eventing, coaching and endurance); and 3 Horses competing at events with a duration of less than five minutes (dressage and show jumping). Flow-volume curves were recorded with an ultrasonic spirometer and TBFVL indices were calculated with specially dedicated software. The sporting usage of the horses had a significant effect on the differentiating ability of TBFVL indices in diagnosing different degrees of RAO. The index TIF50 differentiated control horses from horses with minor RAO in all sporting categories. |
| Address |
Department of Equine Internal Medicine, Faculty of Veterinary Medicine, University of Berne, Langgasstrasse 124, CH-3012 Berne, Switzerland |
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English |
Summary Language |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0042-4900 |
ISBN |
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Conference |
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| Notes |
PMID:12650471 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3725 |
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| Author |
Miksovska, J.; Larsen, R.W. |
| Title |
Photothermal studies of pH induced unfolding of apomyoglobin |
Type |
Journal Article |
| Year |
2003 |
Publication |
Journal of Protein Chemistry |
Abbreviated Journal |
J Protein Chem |
| Volume |
22 |
Issue |
4 |
Pages |
387-394 |
| Keywords |
Acoustics; Animals; Apoproteins/*chemistry/metabolism; Circular Dichroism; Horses; Myocardium/chemistry; Myoglobin/*chemistry/metabolism; Photolysis; Protein Conformation/radiation effects; Protein Denaturation/radiation effects; *Protein Folding; Temperature; Thermodynamics |
| Abstract |
Conformational dynamic and enthalpy changes associated with pH induced unfolding of apomyoglobin were studied using photoacoustic calorimetry and photothermal beam deflection methods. The transition between the native state and the I intermediate was induced by a nanosecond pH jump from o-nitrobenzaldehyde photolysis. Deconvolution of photoacoustic waves indicates two kinetic processes. The fast phase (T < 50 ns) is characterized by a volume expansion of 8.8 ml mol(-1). This process is followed by a volume contraction of about -22 ml mol(-1) (tau approximately 500 ns). Photothermal beam deflection measurements do not reveal any volume changes on the time scale between approximately 100 micros and 5 ms. We associate the volume contraction with structural changes occurring during the transition between the native state and the I intermediate. The lack of any processes on the ms time scale may indicate the absence of structural events involving larger conformational changes of apomyoglobin after the pH jump. |
| Address |
Department of Chemistry, University of South Florida, Tampa, Florida 33620, USA |
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English |
Summary Language |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0277-8033 |
ISBN |
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Medium |
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Conference |
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| Notes |
PMID:13678303 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3780 |
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| Author |
Hoang, L.; Maity, H.; Krishna, M.M.G.; Lin, Y.; Englander, S.W. |
| Title |
Folding units govern the cytochrome c alkaline transition |
Type |
Journal Article |
| Year |
2003 |
Publication |
Journal of Molecular Biology |
Abbreviated Journal |
J Mol Biol |
| Volume |
331 |
Issue |
1 |
Pages |
37-43 |
| Keywords |
Animals; Cytochrome c Group/*chemistry; Horses; Hydrogen/chemistry; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; *Protein Folding; Protein Structure, Tertiary; Spectrum Analysis; Titrimetry |
| Abstract |
The alkaline transition of cytochrome c is a model for protein structural switching in which the normal heme ligand is replaced by another group. Stopped flow data following a jump to high pH detect two slow kinetic phases, suggesting two rate-limiting structure changes. Results described here indicate that these events are controlled by the same structural unfolding reactions that account for the first two steps in the reversible unfolding pathway of cytochrome c. These and other results show that the cooperative folding-unfolding behavior of protein foldons can account for a variety of functional activities in addition to determining folding pathways. |
| Address |
Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA. lhoang@mail.upenn.edu |
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English |
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0022-2836 |
ISBN |
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| Notes |
PMID:12875834 |
Approved |
no |
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Equine Behaviour @ team @ |
Serial |
3781 |
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| Author |
Pinchbeck, G.L.; Clegg, P.D.; Proudman, C.J.; Morgan, K.L.; French, N.P. |
| Title |
Case-control study to investigate risk factors for horse falls in hurdle racing in England and Wales |
Type |
Journal Article |
| Year |
2003 |
Publication |
The Veterinary Record |
Abbreviated Journal |
Vet. Rec. |
| Volume |
152 |
Issue |
19 |
Pages |
583-587 |
| Keywords |
Accidental Falls/*statistics & numerical data; Animals; Athletic Injuries/epidemiology/etiology/*veterinary; Case-Control Studies; England/epidemiology; Horses/*injuries; Risk Factors; Running/injuries; Wales/epidemiology |
| Abstract |
Between March 1, 2000 and August 31, 2001, a case-control study was conducted on 12 racecourses in England and Wales to identify and quantify the risk factors associated with horse falls in hurdle races. The cases and controls were defined so that variables relating to the horse, the jockey, the race and racecourse, and the jump could be considered. The cases were defined as a jumping effort at a hurdle flight that resulted in a fall, and the controls were defined as a successful jump over a hurdle at any of the 12 racecourses within 14 days before or after the case fall. Conditional logistic regression was used to examine the univariable and multivariable relationships between the predictor variables and the risk of falling. The risk of falling was significantly associated with the position of the jump in the race, and with the distance and speed of the race. A horse's previous racing experience and history were also significantly associated with the risk of falling and horses participating in their first hurdle race were at almost five times greater risk of falling than horses that had hurdled before. |
| Address |
Epidemiology Group, Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, Neston CH64 7TE |
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English |
Summary Language |
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Abbreviated Series Title |
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0042-4900 |
ISBN |
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| Notes |
PMID:12762486 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3782 |
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| Author |
Gulotta, M.; Rogatsky, E.; Callender, R.H.; Dyer, R.B. |
| Title |
Primary folding dynamics of sperm whale apomyoglobin: core formation |
Type |
Journal Article |
| Year |
2003 |
Publication |
Biophysical Journal |
Abbreviated Journal |
Biophys J |
| Volume |
84 |
Issue |
3 |
Pages |
1909-1918 |
| Keywords |
Animals; Apoproteins/*chemistry; Crystallography/*methods; Horses; Myocardium/chemistry; Myoglobin/*chemistry; Protein Conformation; *Protein Folding; Species Specificity; Structure-Activity Relationship; Temperature; Whales |
| Abstract |
The structure, thermodynamics, and kinetics of heat-induced unfolding of sperm whale apomyoglobin core formation have been studied. The most rudimentary core is formed at pH(*) 3.0 and up to 60 mM NaCl. Steady state for ultraviolet circular dichroism and fluorescence melting studies indicate that the core in this acid-destabilized state consists of a heterogeneous composition of structures of approximately 26 residues, two-thirds of the number involved for horse heart apomyoglobin under these conditions. Fluorescence temperature-jump relaxation studies show that there is only one process involved in Trp burial. This occurs in 20 micro s for a 7 degrees jump to 52 degrees C, which is close to the limits placed by diffusion on folding reactions. However, infrared temperature jump studies monitoring native helix burial are biexponential with times of 5 micro s and 56 micro s for a similar temperature jump. Both fluorescence and infrared fast phases are energetically favorable but the slow infrared absorbance phase is highly temperature-dependent, indicating a substantial enthalpic barrier for this process. The kinetics are best understood by a multiple-pathway kinetics model. The rapid phases likely represent direct burial of one or both of the Trp residues and parts of the G- and H-helices. We attribute the slow phase to burial and subsequent rearrangement of a misformed core or to a collapse having a high energy barrier wherein both Trps are solvent-exposed. |
| Address |
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA. gulotta@aecom.yu.edu |
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English |
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0006-3495 |
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| Notes |
PMID:12609893 |
Approved |
no |
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Equine Behaviour @ team @ |
Serial |
3783 |
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| Author |
Wallin, L.; Strandberg, E.; Philipsson, J. |
| Title |
Genetic correlations between field test results of Swedish Warmblood Riding Horses as 4-year-olds and lifetime performance results in dressage and show jumping |
Type |
Journal Article |
| Year |
2003 |
Publication |
Livestock Production Science |
Abbreviated Journal |
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| Volume |
82 |
Issue |
1 |
Pages |
61-71 |
| Keywords |
Horse; Performance test; Competition results; Animal model; Heritability; Genetic correlation |
| Abstract |
The main objective of this study was to estimate genetic correlations between traits of young sport horses (4 years old) evaluated in the Swedish Riding Horse Quality Test (RHQT) and later competition results in dressage and show jumping. The data comprised 3708 Warmblood horses born between 1968 and 1982 that had participated in the RHQT as 4-year-olds and 25[punctuation space]605 horses born between 1953 and 1995 with competition records. According to the criteria between 1206 and 1879 horses were common to this two files and were available for the estimations of the genetic correlations. Competition performance traits were cumulative points and cumulative placings received during a horse's lifetime, and a log10 transformation was used to achieve a more normal distribution of the data. Genetic correlations between gait traits scored in the RHQT and competition results in dressage were favourable, in the range 0.63-0.75, and between jumping traits scored in the RHQT and results in show jumping 0.83-0.93. Estimated heritabilities for gait and jumping traits scored in the RHQT were in the range 0.09-0.27 and 0.10-0.18, respectively. Estimated heritabilities for the cumulative points and cumulative placings in dressage and show jumping were 0.17/0.16 and 0.23/0.27, respectively. Thus, the results from the RHQT have proved to be useful for early genetic evaluation and selection of both mares and stallions for sport performance traits. |
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refbase @ user @ |
Serial |
3956 |
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| Author |
Golland, L.C.; Evans, D.L.; McGowan, C.M.; Hodgson, D.R.; Rose, R.J. |
| Title |
The effects of overtraining on blood volumes in standardbred racehorses |
Type |
Journal Article |
| Year |
2003 |
Publication |
Veterinary Journal (London, England : 1997) |
Abbreviated Journal |
Vet J |
| Volume |
165 |
Issue |
3 |
Pages |
228-233 |
| Keywords |
Animals; *Blood Volume; Erythrocytes/*physiology; Hematocrit/veterinary; Horse Diseases/etiology/*physiopathology; Horses; Male; *Physical Conditioning, Animal; Physical Endurance |
| Abstract |
Red blood cell hypervolaemia has been used for diagnosis of overtraining in racehorses, and has been suggested as a mechanism of this cause of loss of racing performance. The effects of overload training (OLT) on the plasma, blood and red cell volumes were investigated in a prospective study in 12 Standardbred horses. Measurements of blood volumes were made after eight and 32 weeks of an exercise training study. Horses were randomly allocated to OLT and control groups (n=6) after 16 weeks of training. Training duration and intensity were increased more rapidly for the OLT group from week 16, until overtraining was diagnosed in week 32.There were no significant effects of OLT on plasma, blood or total red cell volumes between weeks eight and 32. These volumes significantly decreased with time. Maximal haematocrit after exercise was lower (P<0.05) in the OT group in week 32 (0.57+/-0.003% L/L) than in week eight (0.59+/-0.004 L/L). It was concluded that red cell hypervolaemia was not a mechanism for the decrease in capacity for exercise that occurs with overtraining. |
| Address |
Faculty of Veterinary Science, University of Sydney, Sydney, NSW 2006, Australia |
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English |
Summary Language |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
1090-0233 |
ISBN |
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| Notes |
PMID:12672368 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4045 |
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