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Author Andersen, N.H.; Norgaard, A.; Jensen, T.J.; Ulstrup, J. url  openurl
  Title Sequential unfolding of the two-domain protein Pseudomonas stutzeri cytochrome c4 Type Journal Article
  Year 2002 Publication Journal of Inorganic Biochemistry Abbreviated Journal  
  Volume 88 Issue 3-4 Pages 316-327  
  Keywords P. stutzeri cytochrome c4; Sequential unfolding; Di-haem protein; Unfolding  
  Abstract P. stutzeri cytochrome c4 is a di-haem protein, composed of two globular domains each with His-Met coordinated haem, and a hydrogen bond network between the domains. The domain foldings are highly symmetric but with specific differences including structural differences of ligand coordination, and different spin states of the oxidised haem groups. We have studied unfolding of oxidised P. stutzeri cyt c4 induced thermally and by chemical denaturants. Horse heart cyt c was a reference molecule. Isothermal unfolding induced by guanidinium chloride and acid was followed by Soret, α/β, and 701-nm band absorption, and by far-UV circular dichroism spectroscopy. Multifarious patterns emerge, but the two domains clearly unfold sequentially. One phase, assigned to unfolding of the N-terminal domain, proceeds at guanidinium concentrations up to [approximate]1.0 M. This is followed by two overlapping phases at higher concentrations. The intermediate state maintains Fe-Met coordination, assigned to the C-terminal domain. Interdomain interaction is reflected in decreasing values of the cooperativity parameters. Differential scanning calorimetry shows a single peak, but two peaks appear when guanidinium chloride up to 0.4 M is present. This reflects different chemical action in chemical and thermal unfolding. Acid-induced unfolding kinetics was addressed by pH jumps using diode array stopped-flow techniques. Three kinetic phases in the 701 nm Fe-Met marker band, and four phases in the Soret and α/β bands, spanning 4-1000 ms could be distinguished on pH jumps from 7.5 to the range 2.5-3.5. In this range of time and pH cyt c appears to unfold in no more than two phases. Spectral properties of the kinetic intermediates could be identified. Sequential domain unfolding, formation of high-spin states, and an intermediate state with Fe-Met coordination to a single haem group are features of the unfolding kinetics.  
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  Call Number refbase @ user @ Serial 3973  
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Author Voss, B.; Mohr, E.; Krzywanek, H. openurl 
  Title Effects of aqua-treadmill exercise on selected blood parameters and on heart-rate variability of horses Type Journal Article
  Year 2002 Publication Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine Abbreviated Journal J Vet Med A Physiol Pathol Clin Med  
  Volume 49 Issue 3 Pages 137-143  
  Keywords Animals; Electrocardiography/veterinary; Exercise Test/veterinary; Female; Heart Rate/*physiology; Hemoglobins/metabolism; Horses/*physiology; Lactic Acid/blood; Male; Physical Conditioning, Animal/*physiology; Water  
  Abstract The objectives of the present study were to investigate the effects of Aquatraining of horses (aqua-treadmill exercise; treadmill manufactured by Equitech – L.u.S. Equipment, Warendorf, Germany) on selected blood parameters [lactic acid concentration (mmol/l), haemoglobin content (g/l)] and on heart-rate variability (HRV) [heart rate (beats per min; b.p.m.), standard deviation of all NN-intervals (SDNN; ms), normalized power of the low and high frequency band (LFnorm, Hfnorm; au), % recurrence, % determinism and ratio(corr)]. Seven horses performed six exercise tests with different work loads (walking (x = 1.56 +/- 0.08 m/s) and trotting (x = 2.9 +/- 0.13 m/s): dry, water above the carpus and water above the elbow). The standardized test-protocol was: 5 min warm-up at walk while the water was pumped in, followed by the 20-min exercise period at walk or trot, followed by a 5-min walk while pumping out the water. Blood samples were taken prior to each test at rest in the stable, as well as exactly 5 min after the end of the 20-min exercise period. Electrocardiograms were recorded during rest and the 20-min exercise period. Compared to rest, neither the chosen velocities, the two water levels, nor the dry tests led to a significant increase of the lactic acid concentration in any horse. The haemoglobin content showed a significant increase as a result of exercise. Significant differences could be found between the heart rates at rest and the six exercise tests and between the mean of the levels 'walking' and the mean of the levels 'trotting'. An exercise-induced change of HRV was characterized by a decreasing SDNN, a significantly higher LFnorm (sympathetic influence) combined with a significantly lower HF(norm) power (parasympathetic activity) and a rising degree of order (significantly higher % determinism and nearly unchanged % recurrence) and stability (significantly rising ratio(corr)) of the recurrence plot. In conclusion, the used training-protocol for aqua-treadmill exercises only represents a medium-sized aerobic work load for horses, but the different levels of burden were indicated especially by changes in HRV.  
  Address Institute for Veterinary Physiology of the Free University Berlin, Germany  
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  ISSN 0931-184X ISBN Medium  
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  Notes PMID:12019954 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4049  
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Author Previc, F.H. doi  openurl
  Title Thyroid hormone production in chimpanzees and humans: implications for the origins of human intelligence Type Journal Article
  Year 2002 Publication American Journal of Physical Anthropology Abbreviated Journal Am J Phys Anthropol  
  Volume 118 Issue 4 Pages 402-3; discussion 404-5  
  Keywords Animals; Humans; *Intelligence; Pan troglodytes/*metabolism; Species Specificity; Thyroid Hormones/*biosynthesis  
  Abstract  
  Address Northrop Grumman Information Technology, San Antonio, Texas 78228, USA. fred.previc@brooks.af.mil  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9483 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:12124921 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4108  
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Author Griffin, B. openurl 
  Title The use of fecal markers to facilitate sample collection in group-housed cats Type Journal Article
  Year 2002 Publication Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science Abbreviated Journal Contemp Top Lab Anim Sci  
  Volume 41 Issue 2 Pages 51-56  
  Keywords Animals; Behavior, Animal; Biological Markers/*analysis; Cats/*physiology/psychology; Diet/veterinary; Feces/*chemistry; Food Coloring Agents/analysis; Housing, Animal; Individuality; Plastics/analysis; Specimen Handling/methods/*veterinary  
  Abstract The provision of proper social housing is a priority when designing an experiment using domestic cats as laboratory animals. When animals are group-housed, studies requiring analysis of stool samples from individual subjects pose difficulty in sample collection and identification. In this study, commercially available concentrated food colorings (known as bakers pastes) were used as fecal markers in group-housed cats. Cats readily consumed 0.5 ml of bakers paste food coloring once daily in canned cat food. Colorings served as fecal markers by imparting a distinct color to each cat s feces, allowing identification in the litter box. In addition, colored glitter (1/8 teaspoon in canned food) was fed to cats and found to be a reliable fecal marker. Long-term feeding of colorings and glitter was found to be safe and effective at yielding readily identifiable stools.  
  Address Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Alabama 36841, USA  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1060-0558 ISBN Medium  
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  Notes PMID:11958604 Approved no  
  Call Number Equine Behaviour @ team @ Serial 4165  
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Author Casey, R. doi  openurl
  Title Clinical Problems Associated with the Intensive Management of Performance Horses Type Book Chapter
  Year 2002 Publication The Welfare of Horses Abbreviated Journal  
  Volume Issue Pages 19-44  
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  Abstract The physical as well as the behavioural requirements of the horse changed little through the process of domestication. This means that horses kept within an intensively housed environment and used for performance, physically and behaviourally are susceptible to specific clinical conditions, injuries and diseases. In this chapter, physiological and clinical problems such as those causing pain related behaviours and head shaking are discussed. The most commonly associated problems with horses kept in intensive housing conditions or used in specific competitive disciplines are highlighted. Despite the increasing amount of information about injury and disease in the horse, there is little research relating such problems to the situations performance horses have to cope with. This is particularly the case with pain, whose recognition of pain amongst professionals is still variable and often subjective and not widely recognised as a cause of behavioural change.  
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  Call Number Equine Behaviour @ team @ Serial 4376  
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Author Davidson, N.; Harris, P. doi  openurl
  Title Nutrition and Welfare Type Book Chapter
  Year 2002 Publication The Welfare of Horses Abbreviated Journal  
  Volume Issue Pages 45-76  
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  Abstract The horse is a social species living in herds and spending the majority of its time roaming and foraging in a diverse and seasonally-varying environment. As a non-ruminant herbivore it is well suited to a high fibre, low starch diet. Domestication has resulted in a number of benefits to the horse, reflected in its continued prevalence and apparently increased life expectancy, but it has not been without its price. Especially in developed countries, horses kept for leisure purposes (which includes all competition and racing horses) are often confined, possibly away from conspecifics, within a stable for a large proportion of the day. Due to increased energy requirements many horses now receive one to two large meals a day, consisting of feedstuffs with a low water content and often a radically different nutritional profile from the diet that they would be able or would choose to select in the wild. These modern practices have benefits but also potential disadvantages to the horse both nutritionally and behaviourally which may have an impact on welfare. This chapter highlights areas where dietary imbalances or inappropriate feeding practices may potentially have an adverse effect on welfare and gives suggestions on how these may be ameliorated.  
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  Call Number Equine Behaviour @ team @ Serial 4377  
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Author Mills, D.; Clarke, A. doi  openurl
  Title Housing, Management and Welfare Type Book Chapter
  Year 2002 Publication The Welfare of Horses Abbreviated Journal  
  Volume Issue Pages 77-97  
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  Abstract Horses tend to be housed in loose boxes, stalls, barns and shelters for ease of management, however these systems present several possible threats to equine health and welfare. These systems are reviewed together with the concerns they raise. A common system for the evaluation of the welfare of contained animals focuses on the provision of five freedoms. These are freedom from hunger, thirst and malnutrition, from discomfort, from pain, injury and disease, from fear and distress and to express most normal patterns of behaviour. This approach is used to assess the ways in which horse welfare may be compromised by certain housing practices and management regimes. Recommendations as to how these problems can be resolved and to promote good practice are provided.  
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  Call Number Equine Behaviour @ team @ Serial 4378  
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Author Cooper, J.; McGreevy, P. doi  openurl
  Title Stereotypic Behaviour in the Stabled Horse: Causes, Effects and Prevention without Compromising Horse Welfare Type Book Chapter
  Year 2002 Publication The Welfare of Horses Abbreviated Journal  
  Volume Issue Pages 99-124  
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  Abstract Apparently functionless, repetitive behaviour in horses, such as weaving or crib-biting has been difficult to explain for behavioural scientists, horse owners and veterinarians alike. Traditionally activities such as these have been classed amongst the broad descriptor of undesirable stable vices and treatment has centred on prevention of the behaviours per se rather than addressing their underlying causes. In contrast, welfare scientists have described such activities as apparently abnormal stereotypics, claiming they are indicative of poor welfare, citing negative emotions such as boredom, frustration or aversion in the stable environment and even suggesting prevention of the activities alone can lead to increased distress. Our understanding of equine stereotypics has advanced significantly in recent years with epidemiological, developmental and experimental studies identifying those factors closely associated with the performance of stereotypics in stabled horses. These have allowed the development of new treatments based on removing the causal factors, improving the horses“ social and nutritional environment, re-training of horses and their owners and redirection of the activities to less harmful forms. Repetitive activities conventionally seen as undesirable responses to the stable environment, their causal basis and the effectiveness of different approaches to treatment are discussed, both in terms of reducing the behaviour and improving the horse”s quality of life.  
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  Call Number Equine Behaviour @ team @ Serial 4379  
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Author Waran, N.; McGreevy, P.; Casey, R. doi  openurl
  Title Training Methods and Horse Welfare Type Book Chapter
  Year 2002 Publication The Welfare of Horses Abbreviated Journal  
  Volume Issue Pages 151-180  
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  Abstract Many aspects of horse care and handling are based upon convenience and traditional practices. Many of these methods of management and practice do not take into account the natural behaviour of horses. This is despite the belief that although domestic horses are probably more docile, stronger, faster growing and faster moving than their ancestors, they are unlikely to have lost any natural behaviours. The performance or sport horse is expected to perform a wide variety of movements and tasks, some of which are unnatural or exaggerated and most of which must be learned. The term “training” is commonly used to describe the processes whereby the human handler introduces the horse to new situations and associations. Performance horses are often required to tolerate stimuli that are innately aversive or threatening, such as having a person on their backs. They are also trained to respond to a stimulus with often unnatural or over-emphasised behaviour, such as some of the dressage movements. Effective and humane training requires an understanding of the processes underlying behaviour. These include knowledge of behaviour under natural conditions, learning processes, the influence of early experience and motivational forces. Horses differ from the other main companion animal species, namely cats and dogs, in that they are a prey species. They most commonly flee from dangerous and painful situations. Horses readily learn to avoid potentially threatening situations and if their attempts to avoid associated stimuli are prevented, they will often exhibit problem behaviours. In this chapter the history of horse training, the application of learning theory and a knowledge of equine behaviour to training, and innovative training methods are all considered.  
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  Call Number Equine Behaviour @ team @ Serial 4380  
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Author Evans, D. doi  openurl
  Title Welfare of the Racehorse During Exercise Training and Racing Type Book Chapter
  Year 2002 Publication The Welfare of Horses Abbreviated Journal  
  Volume Issue Pages 181-201  
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  Abstract The welfare of horses in training for racing and competition can be compromised by errors of management of many processes. Lameness is usually identified, as the major problem facing horse trainers and high lameness rates in racehorses is a major welfare concern. Recent epidemiological studies have shed light on important environmental risk factors for lameness and catastrophic incidents during training and racing. Another important threat to the welfare of the athletic horse is failure of appropriate preparation of the horse for competition, resulting in earlier fatigue during a race. Fatigue during racing causes sub-optimal performance, increases the likelihood of injury and, in prolonged exercise contributes to exhaustion and even death. Failure to allow appropriate recovery periods after episodes of training and competition also contributes to a state of chronic fatigue. Trainers recognise that affected horses (or “stale” horses) often have mood disturbances and are reluctant to exercise. Continued excessive training and inadequate recovery (termed, over-training) can result in weight loss and poor performance that is not reversed by short-term recovery periods. In events involving prolonged exercise, the performance and welfare of the horse are compromised by inappropriate fluid balance before and during exercise. Failure to properly prepare and maintain fluid balance of endurance horses results in a severe threat to welfare. Pronounced dehydration and hyperthermia can result in exhaustion and death.  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 4381  
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