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Author |
McLaren I.P.L. |
Title |
Animal Learning and Cognition: A neural network approach |
Type |
Journal Article |
Year |
1998 |
Publication |
Trends in Cognitive Sciences |
Abbreviated Journal |
Trends. Cognit. Sci. |
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2 |
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236-236 |
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refbase @ user @ |
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3464 |
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Author |
Vallortigara G. |
Title |
Minds of Their Own |
Type |
Journal Article |
Year |
1998 |
Publication |
Trends in Cognitive Sciences |
Abbreviated Journal |
Trends. Cognit. Sci. |
Volume |
2 |
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118-118 |
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no |
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refbase @ user @ |
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3466 |
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Author |
Mesulam, M.-M. |
Title |
Review article. From sensation to cognition |
Type |
Journal Article |
Year |
1998 |
Publication |
Brain |
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121 |
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1013-1052 |
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no |
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3467 |
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Author |
Mizuguchi, M.; Arai, M.; Ke, Y.; Nitta, K.; Kuwajima, K. |
Title |
Equilibrium and kinetics of the folding of equine lysozyme studied by circular dichroism spectroscopy |
Type |
Journal Article |
Year |
1998 |
Publication |
Journal of Molecular Biology |
Abbreviated Journal |
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Volume |
283 |
Issue |
1 |
Pages |
265-277 |
Keywords |
equine lysozyme; protein folding; molten globule; stopped-flow; folding intermediate |
Abstract |
The equilibrium unfolding and the kinetics of unfolding and refolding of equine lysozyme, a Ca2+-binding protein, were studied by means of circular dichroism spectra in the far and near-ultraviolet regions. The transition curves of the guanidine hydrochloride-induced unfolding measured at 230 nm and 292.5 nm, and for the apo and holo forms of the protein have shown that the unfolding is well represented by a three-state mechanism in which the molten globule state is populated as a stable intermediate. The molten globule state of this protein is more stable and more native-like than that of α-lactalbumin, a homologous protein of equine lysozyme. The kinetic unfolding and refolding of the protein were induced by concentration jumps of the denaturant and measured by stopped-flow circular dichroism. The observed unfolding and refolding curves both agreed well with a single-exponential function. However, in the kinetic refolding reactions below 3 M guanidine hydrochloride, a burst-phase change in the circular dichroism was present, and the burst-phase intermediate in the kinetic refolding is shown to be identical with the molten globule state observed in the equilibrium unfolding. Under a strongly native condition, virtually all the molecules of equine lysozyme transform the structure from the unfolded state into the molten globule, and the subsequent refolding takes place from the molten globule state. The transition state of folding, which may exist between the molten globule and the native states, was characterized by investigating the guanidine hydrochloride concentration-dependence of the rate constants of refolding and unfolding. More than 80% of the hydrophobic surface of the protein is buried in the transition state, so that it is much closer to the native state than to the molten globule in which only 36% of the surface is buried in the interior of the molecule. It is concluded that all the present results are best explained by a sequential model of protein folding, in which the molten globule state is an obligatory folding intermediate on the pathway of folding. |
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3990 |
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Author |
Zohary, D.; Tchernov, E.; Horwitz, L.K. |
Title |
The role of unconscious selection in the domestication of sheep and goats |
Type |
Journal Article |
Year |
1998 |
Publication |
J Zool |
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Volume |
245 |
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Equine Behaviour @ team @ Zohary1998 |
Serial |
6240 |
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Author |
Whiten, A. |
Title |
Imitation of the sequential structure of actions by chimpanzees |
Type |
Journal Article |
Year |
1998 |
Publication |
J Comp Psychol |
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11 |
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Equine Behaviour @ team @ Whiten1998 |
Serial |
6291 |
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Author |
Breitenmoser, U. |
Title |
Large predators in the Alps: the fall and rise of man's competitors |
Type |
Journal Article |
Year |
1998 |
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Biol Conserv |
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83 |
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Equine Behaviour @ team @ Breitenmoser1998 |
Serial |
6450 |
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Author |
Gese, E.M.; Ruff, R.L. |
Title |
Howling by coyotes (Canis latrans): variation among social classes, seasons, and pack sizes |
Type |
Journal Article |
Year |
1998 |
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Can J Zool |
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76 |
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Equine Behaviour @ team @ Gese1998 |
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6462 |
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Author |
Peters, G.; Tembrock, G. |
Title |
Subharmonics, biphonation, and deterministic chaos in mammal vocalizations |
Type |
Journal Article |
Year |
1998 |
Publication |
Bioacoustics |
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9 |
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Equine Behaviour @ team @ Peters1998 |
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6483 |
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Title |
Animal Acoustic Communication: Sound Analysis and Research Methods |
Type |
Book Whole |
Year |
1998 |
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Springer |
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Berlin |
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Equine Behaviour @ team @ ref56 |
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6497 |
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