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Author Kordal, R.J.; Parsons, S.M.
Title Liver alcohol dehydrogenase subunit equivalence studied by rapid sampling of alcohol product formed from sequentially bound [4α-3H]NADH Type Journal Article
Year 1979 Publication Archives of Biochemistry and Biophysics Abbreviated Journal
Volume 194 Issue 2 Pages 439-448
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Abstract Horse liver alcohol dehydrogenase has been claimed to exhibit presteady-state “half-of-the-sites” reactivity with aromatic substrates under some circumstances. To clarify the role of half-of-the-sites reactivity in liver alcohol dehydrogenase the direct sampling of the alcohol product formed immediately after initiation of the reaction was studied using a rapid sampling device and [4α-3H]NADH. Liver alcohol dehydrogenase which contained a very low mole-ratio of [4α-3H]NADH bound to one subunit of the dimer was rapidly mixed with excess 4-(2'-imidazolylazo)benzaldehyde substrate and nonradioactive NADH to initiate the reaction, which was allowed to proceed for a short time before it was quenched. If strong HClO4 quench was used isolation of total free and bound azoalcohol product was possible. If NaOH quench was used isolation only of the azoalcohol product released by the enzyme was possible since most enzyme-bound azoalcohol was reversed back to azoaldehyde by the base. The pH-jump reversal reaction also was characterized spectroscopically by stopped flow technique. Nearly fullsites reactivity was observed for reaction in either direction. Furthermore (4α-3H]NADH bound firstly to one subunit in the dimer reacted essentially identically to NADH bound secondly to the other subunit. Thus, half-of-the-sites reactivity was not observed in these experiments nor did they give any indication of liver alcohol dehydrogenase active site nonequivalence induced by coenzyme binding or reaction.
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Call Number refbase @ user @ Serial (down) 3983
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Author Wilson, M.T.; Silvestrini, M.C.; Morpurgo, L.; Brunori, M.
Title Electron transfer kinetics between Rhus vernicifera stellacyanin and cytochrome c (horse heart cytochrome c and Pseudomonas cytochrome c551) Type Journal Article
Year 1979 Publication Journal of Inorganic Biochemistry Abbreviated Journal J Inorg Biochem
Volume 11 Issue 2 Pages 95-100
Keywords Animals; Copper; Cytochrome c Group/*metabolism; Electron Transport; Kinetics; Metalloproteins/*metabolism; Plant Proteins/*metabolism; *Plants, Toxic; Pseudomonas aeruginosa/*metabolism; Toxicodendron/*metabolism
Abstract The electron transfer reactions between Rhus vernicifera stellacyanin and either horse heart cytochrome c or Pseudomonas aeruginosa cytochrome c551 were investigated by rapid reaction techniques. The time course of electron transfer is monophasic under all conditions, and thus consistent with a simple formulation of the reaction. Both stopped-flow and temperature-jump experiments yield equilibrium constants in reasonable agreement with values calculated from the redox potentials. The differences in reaction rate between the two cytochromes and stellacyanin are discussed in terms of the Marcus theory.
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Language English Summary Language Original Title
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ISSN 0162-0134 ISBN Medium
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Notes PMID:228006 Approved no
Call Number refbase @ user @ Serial (down) 3879
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Author Czerlinski, G.H.; Erickson, J.O.; Theorell, H.
Title Chemical relaxation studies on the horse liver alcohol dehydrogenase system Type Journal Article
Year 1979 Publication Physiological Chemistry and Physics Abbreviated Journal Physiol Chem Phys
Volume 11 Issue 6 Pages 537-569
Keywords Alcohol Oxidoreductases/*metabolism; Animals; Buffers; Electron Transport; Ethanol/metabolism; Horses; Hydrogen-Ion Concentration; Liver/*enzymology; Mathematics; NAD/metabolism; Oscillometry; Osmolar Concentration; Temperature; Time Factors
Abstract Chemical relaxation studies on the system horse liver alcohol dehydrogenase, nicotinamide adenine dinucleotide, and ethanol were conducted observing fluorescence changes between 400 and 500 nm. Temperature-jump experiments were performed at pH 6.5, 7.0, 8.0, and 9.0; concentration-jump experiments at pH 9.0. The reciprocal of the slowest relaxation time was found to be linearly dependent upon the enzyme concentration for relatively low enzyme concentrations, as predicted earlier. Use of the wide pH-range necessitated expression of the four apparent dissociation constants of the catalytic reaction cycle in terms of pH-independent constants. The system was described in terms of only one (or two) catalysis-linked protons not associated with the electron transfer. Protonic steps in a buffered system are in rapid equilibrium, too fast to be measured with the equipment available. Assuming only two of the four bimolecular reaction steps in the four-step cycle are fast compared to the remaining two, six cases may be considered with six expressions for the reciprocal of the slowest relaxation time. Comparison with the experimental data revealed that the bimolecular reaction steps governing the slowest relaxation time change with pH. Above the effective time resolution of the temperature-lump instrument with fluorescence detection (0.1 msec) only one other relaxation time was detectable and only at pH 9. This relaxation time, found to be independent of the concentration of all reactants within experimental error (r = 10 +/- 5 msec), is most likely due to an interconversion among ternary complexes.
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ISSN 0031-9325 ISBN Medium
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Notes PMID:44918 Approved no
Call Number Equine Behaviour @ team @ Serial (down) 3813
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Author Fiske, J.C.
Title Behavior and learning in horses Applications in management and training Type Journal Article
Year 1979 Publication Southwestern Veterinarian Abbreviated Journal Southwestern Veterinarian
Volume 32 Issue Pages 37-44
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Notes Approved no
Call Number Equine Behaviour @ team @ Serial (down) 3568
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Author [No authors listed]
Title International Conference on Environmental Cadmium: an overview Type Conference Article
Year 1979 Publication Environmental Health Perspectives Abbreviated Journal Environ Health Perspect
Volume 28 Issue Pages 297-30
Keywords Animals; Cadmium/*toxicity; Cadmium Poisoning/metabolism; Congresses; Ecology; Environmental Exposure; Environmental Pollutants/*toxicity; Female; Forecasting; Haplorhini; Horses; Humans; Rats; Waste Disposal, Fluid
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ISSN 0091-6765 ISBN Medium
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Notes PMID:39745 Approved no
Call Number Equine Behaviour @ team @ Serial (down) 2694
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Author Ribeiro, H.S.; Larangeira, N.L.; Paiva, F.
Title [Prevalence of Dictyocaulus arnfieldi (Cobbald, 1884) Railiet & Henry 1907, in Pantaneira breed horses of the region of Pocone, MT] Type Journal Article
Year 1979 Publication Arquivos do Instituto Biologico Abbreviated Journal Arq Inst Biol (Sao Paulo)
Volume 46 Issue 3-4 Pages 107-110
Keywords Animals; Brazil; Dictyocaulus/*isolation & purification; Ecology; Female; Horses/*parasitology; Male; Metastrongyloidea/*isolation & purification; Seasons
Abstract The authors sacrificed fifty-five horses originated from the “Pantanal”, lowlands in the State of Mato Grosso in two different periods, droughty period and flooded and they described for the first time the Dictyocaulus arnfieldi in Mato Grosso. Relationship between droughty and flooded periods proved not to occur.
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Language Portuguese Summary Language Original Title Prevalencia de Dictyocaulus arnfieldi (Cobbald, 1884) Railiet & Henry 1907, em cavalos de raca pantaneira da regiao de Pocone, MT
Series Editor Series Title Abbreviated Series Title
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ISSN 0020-3653 ISBN Medium
Area Expedition Conference
Notes PMID:554599 Approved no
Call Number Equine Behaviour @ team @ Serial (down) 2693
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Author Miller, R.
Title Band organisation and stability in Red Desert feral horses Type Book Chapter
Year 1979 Publication Proceedings of a Conference on the Ecology and Behavior of Feral Equids Abbreviated Journal
Volume Issue Pages 113-123
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Publisher University of Wyoming. Place of Publication Laramie Editor R.H. Denniston
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Notes from Professor Hans Klingels Equine Reference List Approved no
Call Number Equine Behaviour @ team @ Serial (down) 2361
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Author Sinclair, A.R.E.
Title Serengeti: Dynamics of an Ecosystem Type Book Whole
Year 1979 Publication Abbreviated Journal
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Call Number Equine Behaviour @ team @ Serial (down) 2346
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Author Keiper, R.R.
Title The behaviour, ecology and social organization of the feral ponies of Assateague Island Type Journal Article
Year 1979 Publication Proc. I. Conf. Sci. Res. Nat. Parks Abbreviated Journal Proc. I. Conf. Sci. Res. Nat. Parks,
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Publisher University of Wyoming Place of Publication Laramie Editor
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Notes Approved no
Call Number Equine Behaviour @ team @ Serial (down) 2309
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Author Powell, R.A.
Title The dog: Its domestication and behavior : By . New York: Garland STPM Press (1978). 296 pp. $24.50 Type Journal Article
Year 1979 Publication Animal Behaviour. Abbreviated Journal Anim. Behav.
Volume 27 Issue Part 1 Pages 318-1211
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Notes Approved no
Call Number Serial (down) 2146
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