Records |
Author |
Kordal, R.J.; Parsons, S.M. |
Title |
Liver alcohol dehydrogenase subunit equivalence studied by rapid sampling of alcohol product formed from sequentially bound [4α-3H]NADH |
Type |
Journal Article |
Year |
1979 |
Publication |
Archives of Biochemistry and Biophysics |
Abbreviated Journal |
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Volume |
194 |
Issue |
2 |
Pages |
439-448 |
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Abstract |
Horse liver alcohol dehydrogenase has been claimed to exhibit presteady-state “half-of-the-sites” reactivity with aromatic substrates under some circumstances. To clarify the role of half-of-the-sites reactivity in liver alcohol dehydrogenase the direct sampling of the alcohol product formed immediately after initiation of the reaction was studied using a rapid sampling device and [4α-3H]NADH. Liver alcohol dehydrogenase which contained a very low mole-ratio of [4α-3H]NADH bound to one subunit of the dimer was rapidly mixed with excess 4-(2'-imidazolylazo)benzaldehyde substrate and nonradioactive NADH to initiate the reaction, which was allowed to proceed for a short time before it was quenched. If strong HClO4 quench was used isolation of total free and bound azoalcohol product was possible. If NaOH quench was used isolation only of the azoalcohol product released by the enzyme was possible since most enzyme-bound azoalcohol was reversed back to azoaldehyde by the base. The pH-jump reversal reaction also was characterized spectroscopically by stopped flow technique. Nearly fullsites reactivity was observed for reaction in either direction. Furthermore (4α-3H]NADH bound firstly to one subunit in the dimer reacted essentially identically to NADH bound secondly to the other subunit. Thus, half-of-the-sites reactivity was not observed in these experiments nor did they give any indication of liver alcohol dehydrogenase active site nonequivalence induced by coenzyme binding or reaction. |
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refbase @ user @ |
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3983 |
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Author |
Wilson, M.T.; Silvestrini, M.C.; Morpurgo, L.; Brunori, M. |
Title |
Electron transfer kinetics between Rhus vernicifera stellacyanin and cytochrome c (horse heart cytochrome c and Pseudomonas cytochrome c551) |
Type |
Journal Article |
Year |
1979 |
Publication |
Journal of Inorganic Biochemistry |
Abbreviated Journal |
J Inorg Biochem |
Volume |
11 |
Issue |
2 |
Pages |
95-100 |
Keywords |
Animals; Copper; Cytochrome c Group/*metabolism; Electron Transport; Kinetics; Metalloproteins/*metabolism; Plant Proteins/*metabolism; *Plants, Toxic; Pseudomonas aeruginosa/*metabolism; Toxicodendron/*metabolism |
Abstract |
The electron transfer reactions between Rhus vernicifera stellacyanin and either horse heart cytochrome c or Pseudomonas aeruginosa cytochrome c551 were investigated by rapid reaction techniques. The time course of electron transfer is monophasic under all conditions, and thus consistent with a simple formulation of the reaction. Both stopped-flow and temperature-jump experiments yield equilibrium constants in reasonable agreement with values calculated from the redox potentials. The differences in reaction rate between the two cytochromes and stellacyanin are discussed in terms of the Marcus theory. |
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0162-0134 |
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PMID:228006 |
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refbase @ user @ |
Serial |
3879 |
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Author |
Czerlinski, G.H.; Erickson, J.O.; Theorell, H. |
Title |
Chemical relaxation studies on the horse liver alcohol dehydrogenase system |
Type |
Journal Article |
Year |
1979 |
Publication |
Physiological Chemistry and Physics |
Abbreviated Journal |
Physiol Chem Phys |
Volume |
11 |
Issue |
6 |
Pages |
537-569 |
Keywords |
Alcohol Oxidoreductases/*metabolism; Animals; Buffers; Electron Transport; Ethanol/metabolism; Horses; Hydrogen-Ion Concentration; Liver/*enzymology; Mathematics; NAD/metabolism; Oscillometry; Osmolar Concentration; Temperature; Time Factors |
Abstract |
Chemical relaxation studies on the system horse liver alcohol dehydrogenase, nicotinamide adenine dinucleotide, and ethanol were conducted observing fluorescence changes between 400 and 500 nm. Temperature-jump experiments were performed at pH 6.5, 7.0, 8.0, and 9.0; concentration-jump experiments at pH 9.0. The reciprocal of the slowest relaxation time was found to be linearly dependent upon the enzyme concentration for relatively low enzyme concentrations, as predicted earlier. Use of the wide pH-range necessitated expression of the four apparent dissociation constants of the catalytic reaction cycle in terms of pH-independent constants. The system was described in terms of only one (or two) catalysis-linked protons not associated with the electron transfer. Protonic steps in a buffered system are in rapid equilibrium, too fast to be measured with the equipment available. Assuming only two of the four bimolecular reaction steps in the four-step cycle are fast compared to the remaining two, six cases may be considered with six expressions for the reciprocal of the slowest relaxation time. Comparison with the experimental data revealed that the bimolecular reaction steps governing the slowest relaxation time change with pH. Above the effective time resolution of the temperature-lump instrument with fluorescence detection (0.1 msec) only one other relaxation time was detectable and only at pH 9. This relaxation time, found to be independent of the concentration of all reactants within experimental error (r = 10 +/- 5 msec), is most likely due to an interconversion among ternary complexes. |
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0031-9325 |
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PMID:44918 |
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Equine Behaviour @ team @ |
Serial |
3813 |
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Author |
Fiske, J.C. |
Title |
Behavior and learning in horses Applications in management and training |
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Journal Article |
Year |
1979 |
Publication |
Southwestern Veterinarian |
Abbreviated Journal |
Southwestern Veterinarian |
Volume |
32 |
Issue |
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Pages |
37-44 |
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Equine Behaviour @ team @ |
Serial |
3568 |
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Author |
[No authors listed] |
Title |
International Conference on Environmental Cadmium: an overview |
Type |
Conference Article |
Year |
1979 |
Publication |
Environmental Health Perspectives |
Abbreviated Journal |
Environ Health Perspect |
Volume |
28 |
Issue |
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Pages |
297-30 |
Keywords |
Animals; Cadmium/*toxicity; Cadmium Poisoning/metabolism; Congresses; Ecology; Environmental Exposure; Environmental Pollutants/*toxicity; Female; Forecasting; Haplorhini; Horses; Humans; Rats; Waste Disposal, Fluid |
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0091-6765 |
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PMID:39745 |
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no |
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Equine Behaviour @ team @ |
Serial |
2694 |
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Author |
Ribeiro, H.S.; Larangeira, N.L.; Paiva, F. |
Title |
[Prevalence of Dictyocaulus arnfieldi (Cobbald, 1884) Railiet & Henry 1907, in Pantaneira breed horses of the region of Pocone, MT] |
Type |
Journal Article |
Year |
1979 |
Publication |
Arquivos do Instituto Biologico |
Abbreviated Journal |
Arq Inst Biol (Sao Paulo) |
Volume |
46 |
Issue |
3-4 |
Pages |
107-110 |
Keywords |
Animals; Brazil; Dictyocaulus/*isolation & purification; Ecology; Female; Horses/*parasitology; Male; Metastrongyloidea/*isolation & purification; Seasons |
Abstract |
The authors sacrificed fifty-five horses originated from the “Pantanal”, lowlands in the State of Mato Grosso in two different periods, droughty period and flooded and they described for the first time the Dictyocaulus arnfieldi in Mato Grosso. Relationship between droughty and flooded periods proved not to occur. |
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Portuguese |
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Prevalencia de Dictyocaulus arnfieldi (Cobbald, 1884) Railiet & Henry 1907, em cavalos de raca pantaneira da regiao de Pocone, MT |
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0020-3653 |
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PMID:554599 |
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Equine Behaviour @ team @ |
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2693 |
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Author |
Miller, R. |
Title |
Band organisation and stability in Red Desert feral horses |
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Book Chapter |
Year |
1979 |
Publication |
Proceedings of a Conference on the Ecology and Behavior of Feral Equids |
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Pages |
113-123 |
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University of Wyoming. |
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Laramie |
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R.H. Denniston |
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from Professor Hans Klingels Equine Reference List |
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Equine Behaviour @ team @ |
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2361 |
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Author |
Sinclair, A.R.E. |
Title |
Serengeti: Dynamics of an Ecosystem |
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Book Whole |
Year |
1979 |
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Equine Behaviour @ team @ |
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2346 |
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Author |
Keiper, R.R. |
Title |
The behaviour, ecology and social organization of the feral ponies of Assateague Island |
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Journal Article |
Year |
1979 |
Publication |
Proc. I. Conf. Sci. Res. Nat. Parks |
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Proc. I. Conf. Sci. Res. Nat. Parks, |
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University of Wyoming |
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Laramie |
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Equine Behaviour @ team @ |
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2309 |
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Author |
Powell, R.A. |
Title |
The dog: Its domestication and behavior : By . New York: Garland STPM Press (1978). 296 pp. $24.50 |
Type |
Journal Article |
Year |
1979 |
Publication |
Animal Behaviour. |
Abbreviated Journal |
Anim. Behav. |
Volume |
27 |
Issue |
Part 1 |
Pages |
318-1211 |
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2146 |
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