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Author |
Czerlinski, G.H.; Erickson, J.O.; Theorell, H. |
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Title |
Chemical relaxation studies on the horse liver alcohol dehydrogenase system |
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Journal Article |
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Year |
1979 |
Publication |
Physiological Chemistry and Physics |
Abbreviated Journal |
Physiol Chem Phys |
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Volume |
11 |
Issue |
6 |
Pages |
537-569 |
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Keywords |
Alcohol Oxidoreductases/*metabolism; Animals; Buffers; Electron Transport; Ethanol/metabolism; Horses; Hydrogen-Ion Concentration; Liver/*enzymology; Mathematics; NAD/metabolism; Oscillometry; Osmolar Concentration; Temperature; Time Factors |
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Abstract |
Chemical relaxation studies on the system horse liver alcohol dehydrogenase, nicotinamide adenine dinucleotide, and ethanol were conducted observing fluorescence changes between 400 and 500 nm. Temperature-jump experiments were performed at pH 6.5, 7.0, 8.0, and 9.0; concentration-jump experiments at pH 9.0. The reciprocal of the slowest relaxation time was found to be linearly dependent upon the enzyme concentration for relatively low enzyme concentrations, as predicted earlier. Use of the wide pH-range necessitated expression of the four apparent dissociation constants of the catalytic reaction cycle in terms of pH-independent constants. The system was described in terms of only one (or two) catalysis-linked protons not associated with the electron transfer. Protonic steps in a buffered system are in rapid equilibrium, too fast to be measured with the equipment available. Assuming only two of the four bimolecular reaction steps in the four-step cycle are fast compared to the remaining two, six cases may be considered with six expressions for the reciprocal of the slowest relaxation time. Comparison with the experimental data revealed that the bimolecular reaction steps governing the slowest relaxation time change with pH. Above the effective time resolution of the temperature-lump instrument with fluorescence detection (0.1 msec) only one other relaxation time was detectable and only at pH 9. This relaxation time, found to be independent of the concentration of all reactants within experimental error (r = 10 +/- 5 msec), is most likely due to an interconversion among ternary complexes. |
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0031-9325 |
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PMID:44918 |
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Equine Behaviour @ team @ |
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3813 |
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Author |
Wilson, M.T.; Silvestrini, M.C.; Morpurgo, L.; Brunori, M. |
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Title |
Electron transfer kinetics between Rhus vernicifera stellacyanin and cytochrome c (horse heart cytochrome c and Pseudomonas cytochrome c551) |
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Journal Article |
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Year |
1979 |
Publication |
Journal of Inorganic Biochemistry |
Abbreviated Journal |
J Inorg Biochem |
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Volume |
11 |
Issue |
2 |
Pages |
95-100 |
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Keywords |
Animals; Copper; Cytochrome c Group/*metabolism; Electron Transport; Kinetics; Metalloproteins/*metabolism; Plant Proteins/*metabolism; *Plants, Toxic; Pseudomonas aeruginosa/*metabolism; Toxicodendron/*metabolism |
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The electron transfer reactions between Rhus vernicifera stellacyanin and either horse heart cytochrome c or Pseudomonas aeruginosa cytochrome c551 were investigated by rapid reaction techniques. The time course of electron transfer is monophasic under all conditions, and thus consistent with a simple formulation of the reaction. Both stopped-flow and temperature-jump experiments yield equilibrium constants in reasonable agreement with values calculated from the redox potentials. The differences in reaction rate between the two cytochromes and stellacyanin are discussed in terms of the Marcus theory. |
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0162-0134 |
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PMID:228006 |
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Call Number |
refbase @ user @ |
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3879 |
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Author |
Kordal, R.J.; Parsons, S.M. |
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Title |
Liver alcohol dehydrogenase subunit equivalence studied by rapid sampling of alcohol product formed from sequentially bound [4α-3H]NADH |
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Journal Article |
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Year |
1979 |
Publication |
Archives of Biochemistry and Biophysics |
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194 |
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2 |
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439-448 |
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Horse liver alcohol dehydrogenase has been claimed to exhibit presteady-state “half-of-the-sites” reactivity with aromatic substrates under some circumstances. To clarify the role of half-of-the-sites reactivity in liver alcohol dehydrogenase the direct sampling of the alcohol product formed immediately after initiation of the reaction was studied using a rapid sampling device and [4α-3H]NADH. Liver alcohol dehydrogenase which contained a very low mole-ratio of [4α-3H]NADH bound to one subunit of the dimer was rapidly mixed with excess 4-(2'-imidazolylazo)benzaldehyde substrate and nonradioactive NADH to initiate the reaction, which was allowed to proceed for a short time before it was quenched. If strong HClO4 quench was used isolation of total free and bound azoalcohol product was possible. If NaOH quench was used isolation only of the azoalcohol product released by the enzyme was possible since most enzyme-bound azoalcohol was reversed back to azoaldehyde by the base. The pH-jump reversal reaction also was characterized spectroscopically by stopped flow technique. Nearly fullsites reactivity was observed for reaction in either direction. Furthermore (4α-3H]NADH bound firstly to one subunit in the dimer reacted essentially identically to NADH bound secondly to the other subunit. Thus, half-of-the-sites reactivity was not observed in these experiments nor did they give any indication of liver alcohol dehydrogenase active site nonequivalence induced by coenzyme binding or reaction. |
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refbase @ user @ |
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3983 |
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Author |
Graham, M.; Letz, R. |
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Title |
Within-species variation in the development of ultrasonic signaling of preweanling rats |
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Journal Article |
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Year |
1979 |
Publication |
Developmental Psychobiology |
Abbreviated Journal |
Dev Psychobiol |
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12 |
Issue |
2 |
Pages |
129-136 |
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Keywords |
Animals; *Animals, Newborn; Animals, Suckling; Body Weight; Cold/adverse effects; Environment; Genotype; Individuality; Object Attachment; Rats; Stress/*physiopathology; Ultrasonics; *Vocalization, Animal |
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The development of litter and individual differences in the rate of ultrasonic signaling of neonatal rats was studied. Systematic variations among litters and individuals emerged, without differential treatment. These differences were not correlated with variations in general development as indexed by body weight. Two experiments using a cross-fostering design showed that litter differences developed independently of variations in postnatal environment. These results indicate that the variations among litters in ultrasound rate have a prenatal, possibly genetic, etiology and may represent reliable indicants of response to environmental stress. |
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0012-1630 |
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PMID:456751 |
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Call Number |
Equine Behaviour @ team @ |
Serial |
4172 |
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Author |
Houpt, K.A. |
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Title |
Intelligence of the horse |
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Journal Article |
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Year |
1979 |
Publication |
Equine Pract. |
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1 |
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20-26 |
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Cited By (since 1996): 5; Export Date: 24 October 2008 |
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Admin @ knut @ |
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4597 |
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Author |
Roberts, S. |
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Title |
Order and Dispute: An Introduction to Legal Anthropology |
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Miscellaneous |
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1979 |
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Equine Behaviour @ team @ |
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4931 |
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Author |
Harrington, F.H.; Mech, L.D. |
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Title |
Wolf howling and its role in territory maintenance |
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1979 |
Publication |
Behaviour |
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68 |
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Equine Behaviour @ team @ Harrington1979 |
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6455 |
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Author |
ANGLE M, et al |
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Title |
Androgenes in feral stallions |
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Conference Volume |
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1979 |
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Symposium on the Ecology and Behavior of wild and feral Equids |
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31-38 |
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Laramie |
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from Prof. Hans Klingels Equine Reference List |
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refbase @ user @ |
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641 |
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Author |
Duncan, P.; Vigne, N. |
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Title |
The effect of group size in horses on the rate of attacks by blood-sucking flies |
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1979 |
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Animal Behaviour. |
Abbreviated Journal |
Anim. Behav. |
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27 |
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Part 2 |
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623-625 |
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763 |
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Author |
Gates, S. |
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Title |
A Study of home ranges of free ranging Exmoore ponies |
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1979 |
Publication |
Mammal Revision |
Abbreviated Journal |
Mamm. Rev. |
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9 |
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:3-18 |
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765 |
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