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Author Rodier, F.
Title [Spectral properties of porcine plasminogen: study of the acidic transition (author's transl)] Type Journal Article
Year 1976 Publication European journal of biochemistry / FEBS Abbreviated Journal Eur J Biochem
Volume 63 Issue 2 Pages 553-562
Keywords Animals; Binding Sites; Guanidines; Hydrogen-Ion Concentration; *Plasminogen; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Swine; Temperature
Abstract The acidic transition of porcine plasminogen, prepared by affinity chromatography, was studied by non-destructive methods. These methods are based on the analysis of the behaviour of the tryptophyls under various conditions. The perturbation of the absorption and emission spectra by pH or temperature and the dynamic quenching of the intrinsic fluorescence are used to obtain information on structural changes which affect the environment of these residues. It is shown that by decreasing pH the fluorescence emission spectra are shifted toward the long wavelengths, with a broadening of the fluorescence band. The same effect can be obtained at constant pH by heating the protein solution. In order to analyze these phenomena, it is assumed that the fluorescence intensities at 355 nm and 328 nm reflect the proportion of the tryptophans which are exposed to the solvent, and buried, respectively. The plot of the ratio of the fluorescence intensities at these wavelengths versus pH or temperature leads to a titration curve showing an unmasking of tryptophans. The proportion of exposed tryptophans is measured by the dynamic fluorescence quenching technique and the data analyzed according to Lehrer. The plot of the fraction of exposed tryptophyls versus pH also shows the unmasking of these chromophores. Thermal perturbation of a solution of plaminogen at neutral pH induces a difference absorption spectrum whose amplitudes at the maxima are proportional to the number of exposed aromatic residues. The comparison with a solution of fully denatured plasminogen in 6 M guanidium chloride, where all the tryptophyls are exposed, shows that the percentage of exposure is equal to 59%. This number is significantly higher than the percentage found by the fluorescence quenching technique (20%), indicating that some tryptophyls are located in crevices, exposed to the solvent but not to the iodide. At acidic pH the absorption difference spectra induced by thermal perturbation are not classical, since they show an inversion and a new band between 300 nm and 305 nm. This band is mentioned in the literature as a minor band of tryptophan which appears when this chromophore is located in an asymmetric environment. On plotting the maximum amplitude of these spectra obtained at acidic pH versus temperature, we obtain a curve indicating that two types of antagonistic interactions are involved in the perturbation of the chromophores spectra. The spectrophotometric titration of plasminogen gives classical absorption difference spectra. By plotting the maximum amplitude at 292 nm versus pH, we obtain a titration curve with an apparent pK of 2.9 units. This pK is acidic which respect to the pK value of a normal carboxyl. This low value can be due to a positively charged group in the neighbourhood of a carboxyl, which interacts with one or more chromophores. When the carboxyl becomes protonated, this positively charged group is free and available to perturb the environment of some chromophores...
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Corporate Author Thesis
Publisher Place of Publication Editor
Language French Summary Language Original Title Proprietes spectrales du plasminogene porcin. Etude de la transition acide
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0014-2956 ISBN Medium
Area Expedition Conference
Notes PMID:4326 Approved no
Call Number Admin @ knut @ Serial (up) 22
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Author Brown, R.F.; Houpt, K.A.; Schryver, H.F.
Title Stimulation of food intake in horses by diazepam and promazine Type Journal Article
Year 1976 Publication Pharmacology, biochemistry, and behavior Abbreviated Journal Pharmacol Biochem Behav
Volume 5 Issue 4 Pages 495-497
Keywords Age Factors; Animals; Diazepam/*pharmacology; Diet; Feeding Behavior/*drug effects; Female; Horses/*physiology; Male; Promazine/*pharmacology; Stimulation, Chemical
Abstract In two adult horses doses of 0.02-0.03 mg/kg diazepam, intravenously, increased 1 hr intake 54-75% above control levels. Intake was stimulated when the diet was a high grain, calorically dense one and also when the diet was a high fiber, calorically dilute one. Two young rapidly growing weanling horses showed an even more pronounced stimulation of intake. Following diazepam 1 hr intake was increased 105-240% above control lelvels. Promazine at a dose of 0.5 mg/kg also stimulated intake in adult horses, but not as markedly as did diazepam. A transquilizer and a neuroleptic appear to have a stimulatory eff upon short-term intake in horses.
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Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0091-3057 ISBN Medium
Area Expedition Conference
Notes PMID:1005496 Approved no
Call Number refbase @ user @ Serial (up) 60
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Author Houpt, K.A.
Title Animal behavior as a subject for veterinary students Type Journal Article
Year 1976 Publication The Cornell veterinarian Abbreviated Journal Cornell Vet
Volume 66 Issue 1 Pages 73-81
Keywords Aggression; Animals; *Behavior, Animal; Cats; Chickens; Dogs; Education, Veterinary; Goats; Horses; Humans; Maternal Behavior; Mice; New York; Sexual Behavior, Animal; Sheep; Sleep; Social Behavior; Social Dominance; Swine
Abstract Knowledge of animal behavior is an important asset for the veterinarian; therefore a course in veterinary animal behavior is offered at the New York State College of Veterinary Medicine as an elective. The course emphasizes the behavior of those species of most interest to the practicing veterinarian: cats, dogs, horses, cows, pigs and sheep. Dominance heirarchies, animal communication, aggressive behavior, sexual behavior and maternal behavior are discussed. Play, learning, diurnal cycles of activity and sleep, and controls of ingestive behavior are also considered. Exotic and zoo animal behaviors are also presented by experts in these fields. The critical periods of canine development are related to the optimum management of puppies. The behavior of feral dogs and horses is described. The role of the veterinarian in preventing cruelty to animals and recognition of pain in animals is emphasized. Whenever possible behavior is observed in the laboratory or on film.
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Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0010-8901 ISBN Medium
Area Expedition Conference
Notes PMID:767053 Approved no
Call Number refbase @ user @ Serial (up) 61
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Author Kirkpatrick, J.F.; Vail, R.; Devous, S.; Schwend, S.; Baker, C.B.; Wiesner, L.
Title Diurnal variation of plasma testosterone in wild stallions Type Journal Article
Year 1976 Publication Biology of reproduction Abbreviated Journal Biol Reprod
Volume 15 Issue 1 Pages 98-101
Keywords Animals; *Circadian Rhythm; Horses/*blood; Male; Montana; Sexual Behavior, Animal; Species Specificity; Testosterone/*blood
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Language English Summary Language Original Title
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Series Volume Series Issue Edition
ISSN 0006-3363 ISBN Medium
Area Expedition Conference
Notes PMID:986195 Approved no
Call Number refbase @ user @ Serial (up) 149
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Author Zentall, S.S.; Zentall, T.R.
Title Activity and task performance of hyperactive children as a function of environmental stimulation Type Journal Article
Year 1976 Publication Journal of consulting and clinical psychology Abbreviated Journal J Consult Clin Psychol
Volume 44 Issue 5 Pages 693-697
Keywords Achievement; Acoustic Stimulation; *Arousal; Auditory Perception; Child; Humans; Hyperkinesis/*etiology; Photic Stimulation; Visual Perception
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Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0022-006X ISBN Medium
Area Expedition Conference
Notes PMID:965541 Approved no
Call Number refbase @ user @ Serial (up) 272
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Author Strayer, F.F.
Title Learning and imitation as a function of social status in macaque monkeys (Macaca nemestrina) Type Journal Article
Year 1976 Publication Animal Behaviour. Abbreviated Journal Anim. Behav.
Volume 24 Issue 4 Pages 835-848
Keywords
Abstract Learning and imitation were examined in animals selected from two groups of sixteen pigtail monkeys. There were significant differences in performance on a cued-alternation task as a function of both social status within the stable group, and prior exposure to a social model. High status animals responded more frequently, but were less successful in acquiring appropriate response delay. Exposure to the model improved response latencies and acquisition of response delay for all subjects. However, model exposure did not improve alternation performance. Results are discussed in terms of prior social experience of the subjects, general learning strategies, and differential sensitivity to multiple reinforcement contingencies. Findings are related to ethological concepts of imitation, and field reports on primate social learning.
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Notes Approved no
Call Number refbase @ user @ Serial (up) 573
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Author Clutton-Brock, T.H.; Greenwood, P.J.; Powell, R.P.
Title Ranks and relationships in Highland ponies and Highland Cows Type Journal Article
Year 1976 Publication Zeitschrift für Tierpsychologie Abbreviated Journal Z. Tierpsychol.
Volume 41 Issue 2 Pages 202-216
Keywords Agonistic Behavior; Animals; *Cattle; Female; Grooming; *Horses; Male; *Social Dominance; Spatial Behavior
Abstract Recent studies of primates have questioned the importance of dominance hierarchies in groups living under natural conditions. In a herd of Highland ponies and one of Highland cattle grazing under free-range conditions on the Isle of Rhum (Inner Hebrides) well defined hierarchies were present. The provision of food produced a marked increase in the frequency of agonistic interactions but had no effect on the rank systems of the two herds. While rank was clearly important in affecting the distribution of agonistic interactions, it was poorly related to behaviour in non-agonistic situations.
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ISSN 0044-3573 ISBN Medium
Area Expedition Conference
Notes PMID:961125 Approved no
Call Number refbase @ user @ Serial (up) 661
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Author Eisenmann V,
Title Le protostylide: valeur systématique et signification phylétique chez les espèces actuelles et fossiles du genre Equus Type Journal Article
Year 1976 Publication Abbreviated Journal Z Säugetierk
Volume 41 Issue Pages 349-365
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Notes from Professor Hans Klingels Equine Reference List Approved no
Call Number Serial (up) 1048
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Author Hagen H,
Title Verhalten beim Steppenzebra Type Journal Article
Year 1976 Publication Abbreviated Journal Die Welt der Tiere
Volume Issue Pages 170-172
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Notes from Professor Hans Klingels Equine Reference List Approved no
Call Number Serial (up) 1157
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Author Hansen Rm,
Title Foods of free-roaming horses in southern New Mexico Type Journal Article
Year 1976 Publication Abbreviated Journal J Range Mgmt
Volume 29 Issue Pages 347
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Notes from Professor Hans Klingels Equine Reference List Approved no
Call Number Serial (up) 1159
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