| Records |
Author  |
Rodier, F. |
| Title |
[Spectral properties of porcine plasminogen: study of the acidic transition (author's transl)] |
Type |
Journal Article |
| Year |
1976 |
Publication |
European journal of biochemistry / FEBS |
Abbreviated Journal |
Eur J Biochem |
| Volume |
63 |
Issue |
2 |
Pages |
553-562 |
| Keywords |
Animals; Binding Sites; Guanidines; Hydrogen-Ion Concentration; *Plasminogen; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Swine; Temperature |
| Abstract |
The acidic transition of porcine plasminogen, prepared by affinity chromatography, was studied by non-destructive methods. These methods are based on the analysis of the behaviour of the tryptophyls under various conditions. The perturbation of the absorption and emission spectra by pH or temperature and the dynamic quenching of the intrinsic fluorescence are used to obtain information on structural changes which affect the environment of these residues. It is shown that by decreasing pH the fluorescence emission spectra are shifted toward the long wavelengths, with a broadening of the fluorescence band. The same effect can be obtained at constant pH by heating the protein solution. In order to analyze these phenomena, it is assumed that the fluorescence intensities at 355 nm and 328 nm reflect the proportion of the tryptophans which are exposed to the solvent, and buried, respectively. The plot of the ratio of the fluorescence intensities at these wavelengths versus pH or temperature leads to a titration curve showing an unmasking of tryptophans. The proportion of exposed tryptophans is measured by the dynamic fluorescence quenching technique and the data analyzed according to Lehrer. The plot of the fraction of exposed tryptophyls versus pH also shows the unmasking of these chromophores. Thermal perturbation of a solution of plaminogen at neutral pH induces a difference absorption spectrum whose amplitudes at the maxima are proportional to the number of exposed aromatic residues. The comparison with a solution of fully denatured plasminogen in 6 M guanidium chloride, where all the tryptophyls are exposed, shows that the percentage of exposure is equal to 59%. This number is significantly higher than the percentage found by the fluorescence quenching technique (20%), indicating that some tryptophyls are located in crevices, exposed to the solvent but not to the iodide. At acidic pH the absorption difference spectra induced by thermal perturbation are not classical, since they show an inversion and a new band between 300 nm and 305 nm. This band is mentioned in the literature as a minor band of tryptophan which appears when this chromophore is located in an asymmetric environment. On plotting the maximum amplitude of these spectra obtained at acidic pH versus temperature, we obtain a curve indicating that two types of antagonistic interactions are involved in the perturbation of the chromophores spectra. The spectrophotometric titration of plasminogen gives classical absorption difference spectra. By plotting the maximum amplitude at 292 nm versus pH, we obtain a titration curve with an apparent pK of 2.9 units. This pK is acidic which respect to the pK value of a normal carboxyl. This low value can be due to a positively charged group in the neighbourhood of a carboxyl, which interacts with one or more chromophores. When the carboxyl becomes protonated, this positively charged group is free and available to perturb the environment of some chromophores... |
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| Language |
French |
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Original Title |
Proprietes spectrales du plasminogene porcin. Etude de la transition acide |
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0014-2956 |
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| Notes |
PMID:4326 |
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| Call Number |
Admin @ knut @ |
Serial |
22 |
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| Author |
Schaller, G.B: |
| Title |
The Serengeti Lion: A Study of Predator-Prey Relations (Wildlife Behavior and Ecology series) |
Type |
Book Whole |
| Year |
1976 |
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University Of Chicago Press |
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Chicago |
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ISBN |
978-0226736402 |
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no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
5159 |
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| Author |
SCHOEN AMS et al, |
| Title |
Behavior of young shetland and welsh ponies |
Type |
Journal Article |
| Year |
1976 |
Publication |
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Abbreviated Journal |
Biol Behav |
| Volume |
1 |
Issue |
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Pages |
192-216 |
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| Notes |
from Professor Hans Klingels Equine Reference List |
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no |
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Serial |
1577 |
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| Author |
Schoen Ams, |
| Title |
Behaviour of young shetland welsh ponies |
Type |
Journal Article |
| Year |
1976 |
Publication |
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Abbreviated Journal |
Biol Behav |
| Volume |
1 |
Issue |
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Pages |
192-216 |
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| Notes |
from Professor Hans Klingels Equine Reference List |
Approved |
no |
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Serial |
1576 |
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| Author |
Smith, J.M.; Parker, G.A. |
| Title |
The logic of asymmetric contests |
Type |
Journal Article |
| Year |
1976 |
Publication |
Animal Behaviour. |
Abbreviated Journal |
Anim. Behav. |
| Volume |
24 |
Issue |
1 |
Pages |
159-175 |
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| Abstract |
A theoretical analysis is made of the evolution of behavioural strategies in contest situations. It is assumed that behaviour will evolve so as to maximize individual fitness. If so, a population will evolve an [`]evolutionarily stable strategy', or ESS, which can be defined as a strategy such that, if all members of a population adopt it, no [`]mutant' strategy can do better. A number of simple models of contest situations are analysed from this point of view. It is concluded that in [`]symmetric' contests the ESS is likely to be a [`]mixed' strategy; that is, either the population will be genetically polymorphic or individuals will be behaviourally variable. Most real contests are probably asymmetric, either in pay-off to the contestants, or in size or weapons, or in some [`]uncorrelated' fashion; i.e. in a fashion which does not substantially bias either the pay-offs or the likely outcome of an escalated contest. An example of an uncorrelated asymmetry is that between the [`]discoverer' of a resource and a [`]late-comer'. It is shown that the ESS in asymmetric contests will usually be to permit the asymmetric cue to settle the contest without escalation. Escalated contests will, however, occur if information to the contestants about the asymmetry is imperfect. |
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0003-3472 |
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no |
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Equine Behaviour @ team @ |
Serial |
5103 |
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| Author |
Smuts Gl, |
| Title |
Population characteristics of Burchell's Zebra in the Krüger National Park |
Type |
Journal Article |
| Year |
1976 |
Publication |
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Abbreviated Journal |
S Afr J Wildl Res |
| Volume |
6 |
Issue |
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Pages |
99-112 |
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| Notes |
from Professor Hans Klingels Equine Reference List |
Approved |
no |
| Call Number |
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Serial |
1614 |
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| Author |
Smuts Gl, |
| Title |
Reproduction in the zebra mare from Krüger National Park |
Type |
Journal Article |
| Year |
1976 |
Publication |
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Abbreviated Journal |
Koedoe |
| Volume |
19 |
Issue |
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Pages |
89-132 |
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| Notes |
from Professor Hans Klingels Equine Reference List |
Approved |
no |
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Serial |
1615 |
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| Author |
Smuts Gl, |
| Title |
Reproduction in the zebra stallion from the Krüger National Park |
Type |
Journal Article |
| Year |
1976 |
Publication |
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Abbreviated Journal |
Zool Afr |
| Volume |
11 |
Issue |
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Pages |
207-220 |
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| Notes |
from Professor Hans Klingels Equine Reference List |
Approved |
no |
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Serial |
1616 |
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| Author |
Strayer, F.F. |
| Title |
Learning and imitation as a function of social status in macaque monkeys (Macaca nemestrina) |
Type |
Journal Article |
| Year |
1976 |
Publication |
Animal Behaviour. |
Abbreviated Journal |
Anim. Behav. |
| Volume |
24 |
Issue |
4 |
Pages |
835-848 |
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| Abstract |
Learning and imitation were examined in animals selected from two groups of sixteen pigtail monkeys. There were significant differences in performance on a cued-alternation task as a function of both social status within the stable group, and prior exposure to a social model. High status animals responded more frequently, but were less successful in acquiring appropriate response delay. Exposure to the model improved response latencies and acquisition of response delay for all subjects. However, model exposure did not improve alternation performance. Results are discussed in terms of prior social experience of the subjects, general learning strategies, and differential sensitivity to multiple reinforcement contingencies. Findings are related to ethological concepts of imitation, and field reports on primate social learning. |
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Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
573 |
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| Author |
Wüst G, |
| Title |
Geburt und perinatales Verhalten beim Steppenzebra |
Type |
Journal Article |
| Year |
1976 |
Publication |
Zoologische Garten |
Abbreviated Journal |
Zool. Garten. |
| Volume |
N.F. Jena 46 |
Issue |
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Pages |
305-352 |
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| Notes |
from Professor Hans Klingels Equine Reference List |
Approved |
no |
| Call Number |
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Serial |
1725 |
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