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Author (up) Hau, J.; Andersson, E.; Carlsson, H.-E.
Title Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces Type Journal Article
Year 2001 Publication Laboratory Animals Abbreviated Journal
Volume 35 Issue 4 Pages 301-306
Abstract Non-invasive measures of immunological markers are an attractive means of stress assessment in laboratory animals. Salivary IgA has been used successfully as a stress marker in the human, and several reports indicate the potential of secretory IgA as a non-invasive measure of stress in animals. The present paper describes the development of an ELISA using commercially available components for the quantification of rat IgA and validation of this assay for the quantification of rat secretory IgA in saliva and faeces. The concentration of IgA in rat saliva varied significantly between duplicate samples obtained from individual rats, and the viscosity and small total volume of rat saliva gave unsatisfactory results for IgA. Faecal IgA was present in high concentrations, and duplicate samples varied by only 2-3%. However, faecal IgA seemed less stable than IgA in other biological compartments, and this finding must be taken into consideration when using quantitative measurements of IgA as a marker of mucous humoral immune status.
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
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Area Expedition Conference
Notes 10.1258/0023677011911822 Approved no
Call Number Equine Behaviour @ team @ Serial 5851
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