| Records |
| Author |
Murphy, M.A.; Waits, L.P.; Kendall, K.C.; Wasser, S.K.; Higbee, J.A.; Bogden, R. |
| Title |
An evaluation of long-term preservation methods for brown bear (Ursus arctos) faecal DNA samples |
Type |
Journal Article |
| Year |
2002 |
Publication |
Conservation Genetics |
Abbreviated Journal |
Conservat. Genet. |
| Volume |
3 |
Issue |
4 |
Pages |
435-440 |
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| Abstract |
Relatively few large-scale faecal DNA studieshave been initiated due to difficulties inamplifying low quality and quantity DNAtemplate. To improve brown bear faecal DNA PCRamplification success rates and to determinepost collection sample longevity, fivepreservation methods were evaluated: 90%ethanol, DETs buffer, silica-dried, oven-driedstored at room temperature, and oven-driedstored at -20 °C. Preservationeffectiveness was evaluated for 50 faecalsamples by PCR amplification of a mitochondrialDNA (mtDNA) locus (~146 bp) and a nuclear DNA(nDNA) locus (~200 bp) at time points of oneweek, one month, three months and six months. Preservation method and storage timesignificantly impacted mtDNA and nDNAamplification success rates. For mtDNA, allpreservation methods had >= 75% success atone week, but storage time had a significantimpact on the effectiveness of the silicapreservation method. Ethanol preserved sampleshad the highest success rates for both mtDNA(86.5%) and nDNA (84%). Nuclear DNAamplification success rates ranged from 26-88%, and storage time had a significant impacton all methods but ethanol. Preservationmethod and storage time should be importantconsiderations for researchers planningprojects utilizing faecal DNA. We recommendpreservation of faecal samples in 90% ethanolwhen feasible, although when collecting inremote field conditions or for both DNA andhormone assays a dry collection method may beadvantageous. |
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1572-9737 |
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Equine Behaviour @ team @ Murphy2002 |
Serial |
6574 |
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| Author |
Plumer, L.; Talvi, T.; Männil, P.; Saarma, U. |
| Title |
Assessing the roles of wolves and dogs in livestock predation with suggestions for mitigating human-wildlife conflict and conservation of wolves |
Type |
Journal Article |
| Year |
2018 |
Publication |
Conservation Genetics |
Abbreviated Journal |
Conservat. Genet. |
| Volume |
19 |
Issue |
3 |
Pages |
665-672 |
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| Abstract |
Predation on livestock is a cause of serious and long-lasting conflict between farmers and wildlife, promoting negative public attitudes and endangering conservation of large carnivores. However, while large carnivores, especially the grey wolf (Canis lupus), are often blamed for killing sheep and other farm animals, free-ranging dogs may also act as predators. To develop appropriate measures for livestock protection, reliable methods for identifying predator species are critical. Identification of predators from visual examination of livestock wounds can be ambiguous and genetic analysis is strongly preferable for accurate species determination. To estimate the proportion of wolves and dogs implicated in sheep predation, we developed a sensitive genetic assay to distinguish between wolves and domestic dogs. A total of 183 predator saliva samples collected from killed sheep in Estonia were analysed. The assay identified the predator species in 143 cases (78%). Sheep were most often killed by wolves (81%); however, predation by dogs was substantial (15%). We compared the molecular results with field observations conducted by local environmental officials and recorded some disagreement, with the latter underestimating the role of dogs. As predator saliva samples collected from prey are often of poor quality, we suggest using mitochondrial DNA as a primary tool to maximise the number of successfully analysed samples. We also suggest adopting forensic DNA analysis more widely in livestock predation assessments as a legislative measure since misidentification that is biased against wolves can be counterproductive for conservation by enhancing conflict with society and leading to increased culling and poaching. |
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1572-9737 |
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Equine Behaviour @ team @ Plumer2018 |
Serial |
6509 |
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Kristjansson, T.; Bjornsdottir, S.; Sigurdsson, A.; Andersson, L.S.; Lindgren, G.; Helyar, S.J.; Klonowski, A.M.; Arnason, T. |
| Title |
The effect of the ‘Gait keeper’ mutation in the DMRT3 gene on gaiting ability in Icelandic horses |
Type |
Journal Article |
| Year |
2014 |
Publication |
Journal of Animal Breeding and Genetics |
Abbreviated Journal |
J. Anim. Breed. Genet. |
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Issue |
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Pages |
n/a-n/a |
| Keywords |
Gaiting ability; genotype effect; genotype probability |
| Abstract |
A nonsense mutation in DMRT3 (‘Gait keeper’ mutation) has a predominant effect on gaiting ability in horses, being permissive for the ability to perform lateral gaits and having a favourable effect on speed capacity in trot. The DMRT3 mutant allele (A) has been found in high frequency in gaited breeds and breeds bred for harness racing, while other horse breeds were homozygous for the wild-type allele (C). The aim of this study was to evaluate further the effect of the DMRT3 nonsense mutation on the gait quality and speed capacity in the multigaited Icelandic horse and demonstrate how the frequencies of the A- and C- alleles have changed in the Icelandic horse population in recent decades. It was confirmed that homozygosity for the DMRT3 nonsense mutation relates to the ability to pace. It further had a favourable effect on scores in breeding field tests for the lateral gait tölt, demonstrated by better beat quality, speed capacity and suppleness. Horses with the CA genotype had on the other hand significantly higher scores for walk, trot, canter and gallop, and they performed better beat and suspension in trot and gallop. These results indicate that the AA genotype reinforces the coordination of ipsilateral legs, with the subsequent negative effect on the synchronized movement of diagonal legs compared with the CA genotype. The frequency of the A-allele has increased in recent decades with a corresponding decrease in the frequency of the C-allele. The estimated frequency of the A-allele in the Icelandic horse population in 2012 was 0.94. Selective breeding for lateral gaits in the Icelandic horse population has apparently altered the frequency of DMRT3 genotypes with a predicted loss of the C-allele in relatively few years. The results have practical implications for breeding and training of Icelandic horses and other gaited horse breeds. |
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1439-0388 |
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no |
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Equine Behaviour @ team @ |
Serial |
5831 |
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Aberle, K.S.; Hamann, H.; Drögemüller, C.; Distl, O. |
| Title |
Genetic diversity in German draught horse breeds compared with a group of primitive, riding and wild horses by means of microsatellite DNA markers |
Type |
Journal Article |
| Year |
2004 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim. Gen. |
| Volume |
35 |
Issue |
4 |
Pages |
270-277 |
| Keywords |
diversity; endangered breeds; genetic variation; horse; microsatellite |
| Abstract |
Summary We compared the genetic diversity and distance among six German draught horse breeds to wild (Przewalski's Horse), primitive (Icelandic Horse, Sorraia Horse, Exmoor Pony) or riding horse breeds (Hanoverian Warmblood, Arabian) by means of genotypic information from 30 microsatellite loci. The draught horse breeds included the South German Coldblood, Rhenish German Draught Horse, Mecklenburg Coldblood, Saxon Thuringa Coldblood, Black Forest Horse and Schleswig Draught Horse. Despite large differences in population sizes, the average observed heterozygosity (Ho) differed little among the heavy horse breeds (0.64�0.71), but was considerably lower than in the Hanoverian Warmblood or Icelandic Horse population. The mean number of alleles (NA) decreased more markedly with declining population sizes of German draught horse breeds (5.2�6.3) but did not reach the values of Hanoverian Warmblood (NA = 6.7). The coefficient of differentiation among the heavy horse breeds showed 11.6% of the diversity between the heavy horse breeds, as opposed to 21.2% between the other horse populations. The differentiation test revealed highly significant genetic differences among all draught horse breeds except the Mecklenburg and Saxon Thuringa Coldbloods. The Schleswig Draught Horse was the most distinct draught horse breed. In conclusion, the study demonstrated a clear distinction among the German draught horse breeds and even among breeds with a very short history of divergence like Rhenish German Draught Horse and its East German subpopulations Mecklenburg and Saxon Thuringa Coldblood. |
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Blackwell Science Ltd |
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Edition |
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| ISSN |
1365-2052 |
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no |
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Equine Behaviour @ team @ |
Serial |
5184 |
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Hedrick, P.W.; Parker, K.M.; Miller, E.L.; Miller, P.S. |
| Title |
Major Histocompatibility Complex Variation in the Endangered Przewalski's Horse |
Type |
Journal Article |
| Year |
1999 |
Publication |
Genetics |
Abbreviated Journal |
Genetics |
| Volume |
152 |
Issue |
4 |
Pages |
1701-1710 |
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| Abstract |
The major histocompatibility complex (MHC) is a fundamental part of the vertebrate immune system, and the high variability in many MHC genes is thought to play an essential role in recognition of parasites. The Przewalski's horse is extinct in the wild and all the living individuals descend from 13 founders, most of whom were captured around the turn of the century. One of the primary genetic concerns in endangered species is whether they have ample adaptive variation to respond to novel selective factors. In examining 14 Przewalski's horses that are broadly representative of the living animals, we found six different class II DRB major histocompatibility sequences. The sequences showed extensive nonsynonymous variation, concentrated in the putative antigen-binding sites, and little synonymous variation. Individuals had from two to four sequences as determined by single-stranded conformation polymorphism (SSCP) analysis. On the basis of the SSCP data, phylogenetic analysis of the nucleotide sequences, and segregation in a family group, we conclude that four of these sequences are from one gene (although one sequence codes for a nonfunctional allele because it contains a stop codon) and two other sequences are from another gene. The position of the stop codon is at the same amino-acid position as in a closely related sequence from the domestic horse. Because other organisms have extensive variation at homologous loci, the Przewalski's horse may have quite low variation in this important adaptive region. N1 - |
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no |
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Equine Behaviour @ team @ |
Serial |
5043 |
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| Author |
Oakenfull, E.A.; Ryder, O.A. |
| Title |
Mitochondrial control region and 12S rRNA variation in Przewalski's horse (Equus przewalskii) |
Type |
Journal Article |
| Year |
1998 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
| Volume |
29 |
Issue |
6 |
Pages |
456-459 |
| Keywords |
Animals; DNA, Mitochondrial/*genetics; Female; *Genetic Variation; Horses/*genetics; Male; Pedigree; RNA, Ribosomal/*genetics |
| Abstract |
Variation in the control region and the 12S rRNA gene of all surviving mitochondrial lineages of Przewalski's horse was investigated. Variation is low despite the present day population being descended from 13 individuals probably representing animals from three different regions of its range. Phylogenetic comparison of these sequences, with sequences for the domestic horse, does not resolve the ancestral status of either horse. |
| Address |
Center for Reproduction of Endangered Species, Zoological Society of San Diego, CA 92112, USA |
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English |
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0268-9146 |
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| Notes |
PMID:9883508 |
Approved |
no |
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Equine Behaviour @ team @ |
Serial |
5040 |
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Oakenfull, E.A.; Lim, H.; Ryder, O. |
| Title |
A survey of equid mitochondrial DNA: Implications for the evolution, genetic diversity and conservation of Equus |
Type |
Journal Article |
| Year |
2000 |
Publication |
Conservation Genetics |
Abbreviated Journal |
Conservat Genet |
| Volume |
1 |
Issue |
4 |
Pages |
341-355 |
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| Abstract |
The evolution, taxonomy and conservation of the genus Equuswere investigated by examining the mitochondrial DNA sequences of thecontrol region and 12S rRNA gene. The phylogenetic analysis of thesesequences provides further evidence that the deepest node in thephylogeny of the extant species is a divergence between twolineages; one leading to the ancestor of modern horses (E.ferus, domestic and przewalskii) and the other to thezebra and ass ancestor, with the later speciation events of the zebrasand asses occurring either as one or more rapid radiations, or withextensive secondary contact after speciation. Examination of the geneticdiversity within species suggested that two of the E. hemionussubspecies (E. h. onager and E. h. kulan) onlyrecently diverged, and perhaps, are insufficiently different to beclassified as separate subspecies. The genetic divergence betweendomestic and wild forms of E. ferus (horse) and E.africanus (African ass) was no greater than expected within anequid species. In E. burchelli (plains zebra) there was anindication of mtDNA divergence between populations increasing withdistance. The implications of these results for equid conservation arediscussed and recommendations are made for conservation action. |
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no |
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Equine Behaviour @ team @ |
Serial |
5039 |
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| Author |
Wallner, B.; Brem, G.; Muller, M.; Achmann, R. |
| Title |
Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalskii and Equus caballus |
Type |
Journal Article |
| Year |
2003 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
| Volume |
34 |
Issue |
6 |
Pages |
453-456 |
| Keywords |
Animals; Base Sequence; DNA, Mitochondrial/genetics; Genetic Variation/*genetics; Horses/classification/*genetics; Male; Molecular Sequence Data; Phylogeny; Probability; Species Specificity; Y Chromosome/*genetics |
| Abstract |
The phylogenetic relationship between Equus przewalskii and E. caballus is often a matter of debate. Although these taxa have different chromosome numbers, they do not form monophyletic clades in a phylogenetic tree based on mtDNA sequences. Here we report sequence variation from five newly identified Y chromosome regions of the horse. Two fixed nucleotide differences on the Y chromosome clearly display Przewalski's horse and domestic horse as sister taxa. At both positions the Przewalski's horse haplotype shows the ancestral state, in common with the members of the zebra/ass lineage. We discuss the factors that may have led to the differences in mtDNA and Y-chromosomal observations. |
| Address |
Institut fur Tierzucht und Genetik, Veterinarmedizinische Universitat Wien, Veterinarplatz, Wien, Austria. wallner@i122server.vu-wien.ac.at |
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English |
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0268-9146 |
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| Notes |
PMID:14687077 |
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no |
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Equine Behaviour @ team @ |
Serial |
5038 |
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| Author |
Houpt, K. A.; Kusonose, R. |
| Title |
Genetic of behaviour |
Type |
Book Chapter |
| Year |
2000 |
Publication |
Genetics of the Horse |
Abbreviated Journal |
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Issue |
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Pages |
281-306 |
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Cab Intl |
Place of Publication |
Wallingford Oxfordshire |
Editor |
Bowling,A. T. ; Ruvinsky, A. |
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978-0851994291 |
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no |
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Equine Behaviour @ team @ |
Serial |
5021 |
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| Author |
Houpt, K.; Kusunose, R. |
| Title |
Genetics of behaviour. |
Type |
Book Chapter |
| Year |
2000 |
Publication |
The Genetics of the Horse |
Abbreviated Journal |
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Issue |
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Pages |
281-306 |
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CABI Publishing |
Place of Publication |
New York |
Editor |
Bowling, A.T., Ruvinsky, A. |
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9780851994291 |
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Equine Behaviour @ team @ |
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4821 |
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