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Author (up) Murphy, M.A.; Waits, L.P.; Kendall, K.C.; Wasser, S.K.; Higbee, J.A.; Bogden, R. url  doi
openurl 
  Title An evaluation of long-term preservation methods for brown bear (Ursus arctos) faecal DNA samples Type Journal Article
  Year 2002 Publication Conservation Genetics Abbreviated Journal Conservat. Genet.  
  Volume 3 Issue 4 Pages 435-440  
  Keywords  
  Abstract Relatively few large-scale faecal DNA studieshave been initiated due to difficulties inamplifying low quality and quantity DNAtemplate. To improve brown bear faecal DNA PCRamplification success rates and to determinepost collection sample longevity, fivepreservation methods were evaluated: 90%ethanol, DETs buffer, silica-dried, oven-driedstored at room temperature, and oven-driedstored at -20 °C. Preservationeffectiveness was evaluated for 50 faecalsamples by PCR amplification of a mitochondrialDNA (mtDNA) locus (~146 bp) and a nuclear DNA(nDNA) locus (~200 bp) at time points of oneweek, one month, three months and six months. Preservation method and storage timesignificantly impacted mtDNA and nDNAamplification success rates. For mtDNA, allpreservation methods had >= 75% success atone week, but storage time had a significantimpact on the effectiveness of the silicapreservation method. Ethanol preserved sampleshad the highest success rates for both mtDNA(86.5%) and nDNA (84%). Nuclear DNAamplification success rates ranged from 26-88%, and storage time had a significant impacton all methods but ethanol. Preservationmethod and storage time should be importantconsiderations for researchers planningprojects utilizing faecal DNA. We recommendpreservation of faecal samples in 90% ethanolwhen feasible, although when collecting inremote field conditions or for both DNA andhormone assays a dry collection method may beadvantageous.  
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  ISSN 1572-9737 ISBN Medium  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Murphy2002 Serial 6574  
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Author (up) Taberlet, P.; Waits, L.P.; Luikart, G. url  doi
openurl 
  Title Noninvasive genetic sampling: look before you leap Type Journal Article
  Year 1999 Publication Trends in Ecology & Evolution Abbreviated Journal Trends Ecol. Evol  
  Volume 14 Issue 8 Pages 323-327  
  Keywords Hairs; Feces; Feathers; Allelic dropout; Individual identification; Conservation genetics; Behavioural ecology; Pilot study; Microsatellites; Probability of identity  
  Abstract Noninvasive sampling allows genetic studies of free-ranging animals without the need to capture or even observe them, and thus allows questions to be addressed that cannot be answered using conventional methods. Initially, this sampling strategy promised to exploit fully the existing DNA-based technology for studies in ethology, conservation biology and population genetics. However, recent work now indicates the need for a more cautious approach, which includes quantifying the genotyping error rate. Despite this, many of the difficulties of noninvasive sampling will probably be overcome with improved methodology.  
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  Series Volume Series Issue Edition  
  ISSN 0169-5347 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6573  
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