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| Author | Brinkmann, L.; Gerken, M.; Riek, A. | ||||
| Title | Energetic adaptations of Shetland pony mares | Type | Conference Article | ||
| Year | 2015 | Publication | Proceedings of the 3. International Equine Science Meeting | Abbreviated Journal  |
Proc. 3. Int. Equine. Sci. Mtg |
| Volume | Issue | Pages | |||
| Keywords | Body temperature, Energy expenditure, Food restriction, Hypometabolism, Locomotor activity, Shetland pony | ||||
| Abstract | Recent results suggest that wild Northern herbivores exhibit signs of a hypometabolism during times of low ambient temperature and food shortage in order to reduce their energetic needs. However, there are speculations that domestic animals lost the ability to reduce energy expenditure. To examine energetic and behavioural responses 10 Shetland pony mares were exposed to different environmental conditions (summer and winter). During winter ponies were allocated into two groups receiving two different food quantities (60% and 100% of maintenance energy requirement). We measured the field metabolic rate, water turn over, body temperature, locomotor activity, lying time, resting heart rate, body mass and body condition score. In summer, the field metabolic rate of all ponies (FMR; 63.4±15.0 MJ/day) was considerably higher compared with food restricted and control animals in winter (24.6±7.8 and 15.0±1.1 MJ/day, respectively). Furthermore, during summer, locomotor activity, resting heart rate and total water turnover were significantly elevated (P<0.001) compared with winter. Animals receiving a reduced amount of food (N=5) reduced their FMR by 26% compared with control animals (N=5) to compensate for the decreased energy supply. Furthermore, resting heart rate, body mass and body condition score were lower(29.2±2.7 beats/min, 140±22 kg and 3.0±1.0 points, respectively) than in control animals (36.8±41 beats/min, 165±31 kg, 4.4±0.7 points; P<0.05). While no difference could be found in the observed behaviour, nocturnal hypothermia was elevated in restrictively fed animals. Our results indicate that ponies adapt to different climatic conditions by changing their metabolic rate, behaviour and some physiological parameters. When exposed to energy shortage, ponies, like wild herbivores, exhibited hypometabolism and nocturnal hypothermia. Keywords: Body temperature, Energy expenditure, Food restriction, Hypometabolism, Locomotor activity, Shetland pony |
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| Address | |||||
| Corporate Author | Brinkmann, L. | Thesis | |||
| Publisher | Place of Publication | Editor | |||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | ISBN | Medium | |||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 5893 | ||
| Permanent link to this record | |||||
| Author | Ballew, R.M.; Sabelko, J.; Gruebele, M. | ||||
| Title | Direct observation of fast protein folding: the initial collapse of apomyoglobin | Type | Journal Article | ||
| Year | 1996 | Publication | Proceedings of the National Academy of Sciences of the United States of America | Abbreviated Journal |
Proc. Natl. Acad. Sci. U.S.A. |
| Volume | 93 | Issue | 12 | Pages | 5759-5764 |
| Keywords | Animals; Apoproteins/*chemistry; Circular Dichroism; Horses; Kinetics; Muscle, Skeletal/chemistry; Myoglobin/*chemistry; *Protein Folding; Spectrometry, Fluorescence; Spectrophotometry, Infrared; Temperature | ||||
| Abstract | The rapid refolding dynamics of apomyoglobin are followed by a new temperature-jump fluorescence technique on a 15-ns to 0.5-ms time scale in vitro. The apparatus measures the protein-folding history in a single sweep in standard aqueous buffers. The earliest steps during folding to a compact state are observed and are complete in under 20 micros. Experiments on mutants and consideration of steady-state CD and fluorescence spectra indicate that the observed microsecond phase monitors assembly of an A x (H x G) helix subunit. Measurements at different viscosities indicate diffusive behavior even at low viscosities, in agreement with motions of a solvent-exposed protein during the initial collapse. | ||||
| Address | School of Chemical Sciences and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana, 61801, USA | ||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | English | Summary Language | Original Title | ||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 0027-8424 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | PMID:8650166 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 3798 | ||
| Permanent link to this record | |||||
| Author | Permyakov, S.E.; Khokhlova, T.I.; Nazipova, A.A.; Zhadan, A.P.; Morozova-Roche, L.A.; Permyakov, E.A. | ||||
| Title | Calcium-binding and temperature induced transitions in equine lysozyme: new insights from the pCa-temperature “phase diagrams” | Type | Journal Article | ||
| Year | 2006 | Publication | Proteins | Abbreviated Journal |
Proteins |
| Volume | 65 | Issue | 4 | Pages | 984-998 |
| Keywords | Animals; Apoproteins/chemistry/metabolism; Binding Sites; Calcium/chemistry/*metabolism; Cattle; Edetic Acid/metabolism; Horses/metabolism; Hydrogen-Ion Concentration; Lactalbumin/chemistry/metabolism; Muramidase/*chemistry/*metabolism; Protein Denaturation; Spectrometry, Fluorescence; *Temperature; Thermodynamics; Tryptophan/chemistry/metabolism | ||||
| Abstract | The most universal approach to the studies of metal binding properties of single-site metal binding proteins, i.e., construction of a “phase diagram” in coordinates of free metal ion concentration-temperature, has been applied to equine lysozyme (EQL). EQL has one relatively strong calcium binding site and shows two thermal transitions, but only one of them is Ca(2+)-dependent. It has been found that the Ca(2+)-dependent behavior of the low temperature thermal transition (I) of EQL can be adequately described based upon the simplest four-states scheme of metal- and temperature-induced structural changes in a protein. All thermodynamic parameters of this scheme were determined experimentally and used for construction of the EQL phase diagram in the pCa-temperature space. Comparison of the phase diagram with that for alpha-lactalbumin (alpha-LA), a close homologue of lysozyme, allows visualization of the differences in thermodynamic behavior of the two proteins. The thermal stability of apo-EQL (transition I) closely resembles that for apo-alpha-LA (mid-temperature 25 degrees C), while the thermal stabilities of their Ca(2+)-bound forms are almost indistinguishable. The native state of EQL has three orders of magnitude lower affinity for Ca(2+) in comparison with alpha-LA while its thermally unfolded state (after the I transition) has about one order lower (K = 15M(-1)) affinity for calcium. Circular dichroism studies of the apo-lysozyme state after the first thermal transition show that it shares common features with the molten globule state of alpha-LA. | ||||
| Address | Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow region 142290, Russia | ||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | English | Summary Language | Original Title | ||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 1097-0134 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | PMID:17022083 | Approved | no | ||
| Call Number | Serial | 1858 | |||
| Permanent link to this record | |||||
| Author | Chmel, L.; Hasilikova, A.; Hrasko, J.; Vlacilikova, A. | ||||
| Title | The influence of some ecological factors on keratinophilic fungi in the soil | Type | Journal Article | ||
| Year | 1972 | Publication | Sabouraudia | Abbreviated Journal |
Sabouraudia |
| Volume | 10 | Issue | 1 | Pages | 26-34 |
| Keywords | Animals; Arthrodermataceae/growth & development/isolation & purification/metabolism; Carbohydrates; Czechoslovakia; Ecology; Fungi/growth & development/*isolation & purification/metabolism; Hair; Horses; Humic Substances; Humidity; Keratins/metabolism; Microsporum/isolation & purification; Mitosporic Fungi/isolation & purification; Phosphates; Seasons; Soil; *Soil Microbiology; Species Specificity; Temperature; Trichophyton/isolation & purification | ||||
| Abstract | |||||
| Address | |||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | English | Summary Language | Original Title | ||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 0036-2174 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | PMID:5063162 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 2719 | ||
| Permanent link to this record | |||||
| Author | Hughes, K.L.; Sulaiman, I. | ||||
| Title | The ecology of Rhodococcus equi and physicochemical influences on growth | Type | Journal Article | ||
| Year | 1987 | Publication | Veterinary Microbiology | Abbreviated Journal |
Vet Microbiol |
| Volume | 14 | Issue | 3 | Pages | 241-250 |
| Keywords | Animals; Feces/microbiology; Horses; Hydrogen-Ion Concentration; Rhodococcus/*growth & development; *Soil Microbiology; Temperature | ||||
| Abstract | Growth of Rhodococcus equi was studied in vitro. Optimal growth occurred under aerobic conditions between pH 7.0 and 8.5, at 30 degrees C. R. equi survived better in a neutral soil (pH 7.3) than it did in two acid soils (pH less than 5.5). It grew substantially better in soils enriched with faeces than in soils alone. Simple organic acids in horse dung, especially acetate and propionate, appear to be important in supporting growth of R. equi in the environment. The ecology of R. equi can be best explained by an environmental cycle allowing its proliferation in dung, influenced by management, grazing behaviour and prevailing climatic conditions. Preventive measures should be aimed at reducing or avoiding focal areas of faecal contamination in the environment. | ||||
| Address | School of Veterinary Science, University of Melbourne, Parkville, Vic., Australia | ||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | English | Summary Language | Original Title | ||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 0378-1135 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | PMID:3672866 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 2678 | ||
| Permanent link to this record | |||||
| Author | Natalini, C.C.; Robinson, E.P. | ||||
| Title | Effects of epidural opioid analgesics on heart rate, arterial blood pressure, respiratory rate, body temperature, and behavior in horses | Type | Journal Article | ||
| Year | 2003 | Publication | Veterinary Therapeutics : Research in Applied Veterinary Medicine | Abbreviated Journal |
Vet Ther |
| Volume | 4 | Issue | 4 | Pages | 364-375 |
| Keywords | 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer/administration & dosage/pharmacology; Alfentanil/administration & dosage/pharmacology; Analgesics, Opioid/administration & dosage/*pharmacology; Anesthesia, Epidural/*veterinary; Animals; Behavior, Animal/drug effects; Blood Pressure/drug effects; Body Temperature/drug effects; Butorphanol/administration & dosage/pharmacology; Cross-Over Studies; Female; Heart Rate/drug effects; Horses/*physiology; Injections, Epidural/veterinary; Male; Morphine/administration & dosage/pharmacology; Respiration/drug effects; Tramadol/administration & dosage/pharmacology | ||||
| Abstract | Heart rate, arterial blood pressures, respiratory rate, body temperature, and central nervous system excitement were compared before and after epidural administration of morphine (0.1 mg/kg), butorphanol (0.08 mg/kg), alfentanil (0.02 mg/kg), tramadol (1.0 mg/kg), the k-opioid agonist U50488H (0.08 mg/kg), or sterile water using an incomplete Latin square crossover design in five conscious adult horses. Treatments were administered into the first intercoccygeal epidural space. Significant (P <.05) reductions in respiratory rate were detected after epidural administration of morphine, alfentanil, U50488H, and sterile water. Additionally, significant (P <.05) head ptosis was observed within the first hour after administration of morphine, U50488H, and tramadol, but neither of these changes appeared to be of clinical significance. No treatment-related changes in motor activity or behavior were observed. | ||||
| Address | Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA | ||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | English | Summary Language | Original Title | ||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 1528-3593 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | PMID:15136978 | Approved | no | ||
| Call Number | Serial | 1902 | |||
| Permanent link to this record | |||||