| Records |
| Author |
Alexander, F.; Horner, M.W.; Moss, M.S. |
| Title |
The salivary secretion and clearance in the horse of chloral hydrate and its metabolites |
Type |
Journal Article |
| Year |
1967 |
Publication |
Biochemical pharmacology |
Abbreviated Journal |
Biochem Pharmacol |
| Volume |
16 |
Issue |
7 |
Pages  |
1305-1311 |
| Keywords |
Animals; Chloral Hydrate/blood/*metabolism/urine; Chromatography, Gas; Ethanol/blood/urine; Horses; Male; Parotid Gland/metabolism; Saliva/*analysis; Trichloroacetic Acid/blood/urine |
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English |
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Edition |
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| ISSN |
0006-2952 |
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| Notes |
PMID:6053598 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
118 |
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| Author |
Gröschl, M.; Wagner, R.; Rauh, M.; Dörr, H.G. |
| Title |
Stability of salivary steroids: the influences of storage, food and dental care |
Type |
Journal Article |
| Year |
2001 |
Publication |
Steroids |
Abbreviated Journal |
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| Volume |
66 |
Issue |
10 |
Pages |
737-741 |
| Keywords |
Cortisol; 17OH-Progesterone; Progesterone; Saliva; Stability |
| Abstract |
We studied influences of dental care, food and storage on the reproducibility of salivary steroid levels. Cortisol (F), 17OH-progesterone (17OHP) and Progesterone (P) were measured using adapted commercial radioimmunoassays. Saliva samples of healthy adults (n = 15; m:8; f:7) were collected directly before and after dental care, and directly before and after breakfast with various foodstuffs. A second experiment investigated stability of steroids under different storage conditions. Four series of identical saliva portions (I: Native saliva; II: Centrifuged saliva; III: Saliva with trifluor acetate (TFA); IV: Saliva with 0.5% NaN3) were stored at room temperature and at 4°C for up to three weeks. To demonstrate influences of repeated thawing and re-freezing of saliva on steroid values, saliva samples (n = 15) were divided into identical portions. These portions were frozen and re-thawed up to 5 times before measurement. Neither dental care nor intake of bread or milk effected the reproducibility of F, 170HP, and P. Steroid levels decreased significantly in the course of three weeks under different storage conditions (P < 0.001). This decrease was clinically relevant from the second week onward, with exception of NaN3 treated samples. After repeated freezing and re-thawing 17OHP and P decreased slightly (about 5%). Only F decreased significantly after the third thawing (P < 0.001). The results show the usefulness of standardized handling of saliva samples for improving reproducibility and reliability of salivary steroid measurements. |
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0039-128x |
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no |
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Equine Behaviour @ team @ |
Serial |
5561 |
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| Author |
Alexander, F. |
| Title |
A study of parotid salivation in the horse |
Type |
Journal Article |
| Year |
1966 |
Publication |
The Journal of physiology |
Abbreviated Journal |
J Physiol |
| Volume |
184 |
Issue |
3 |
Pages |
646-656 |
| Keywords |
Animals; Atropine/*pharmacology; Bicarbonates/metabolism; Calcium/metabolism; Chlorides/metabolism; Horses; Mastication/*physiology; Parotid Gland/*physiology; Pilocarpine/*pharmacology; Potassium/metabolism; Salivation/*drug effects; Sodium/metabolism; Tetracaine/*pharmacology |
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English |
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Edition |
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| ISSN |
0022-3751 |
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| Notes |
PMID:5963737 |
Approved |
no |
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refbase @ user @ |
Serial |
119 |
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| Author |
Peeters, M.; Sulon, J.; Beckers, J.-F.; Ledoux, D.; Vandenheede, M. |
| Title |
Comparison between blood serum and salivary cortisol concentrations in horses using an adrenocorticotropic hormone challenge |
Type |
Journal Article |
| Year |
2011 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
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| Volume |
43 |
Issue |
4 |
Pages |
487-493 |
| Keywords |
horse; cortisol; ACTH challenge; saliva; stress |
| Abstract |
Reasons for performing study: In horses, serum cortisol concentration is considered to provide an indirect measurement of stress. However, it includes both free and bound fractions. The sampling method is also invasive and often stressful. This is not the case for salivary cortisol, which is collected using a more welfare-friendly method and represents a part of the free cortisol fraction, which is the biologically active form. Objectives: To compare salivary and serum cortisol assays in horses, in a wide range of concentrations, using an adrenocorticotropic hormone (ACTH) stimulation test, in order to validate salivary cortisol for stress assessment in horse. Methods: In 5 horses, blood samples were drawn using an i.v. catheter. Saliva samples were taken using swabs. Cortisol was assayed by radioimmunoassay. All data were treated with a regression method, which pools and analyses data from multiple subjects for linear analysis. Results: Mean ± s.d. cortisol concentrations measured at rest were 188.81 ± 51.46 nmol/l in serum and 1.19 ± 0.54 nmol/l in saliva. They started increasing immediately after ACTH injection and peaks were reached after 96 ± 16.7 min in serum (356.98 ± 55.29 nmol/l) and after 124 ± 8.9 min in saliva (21.79 ± 7.74 nmol/l, P<0.05). Discharge percentages were also different (225% in serum and 2150% in saliva, P<0.05). Correlation between serum and salivary cortisol concentrations showed an adjusted r2= 0.80 (P<0.001). The strong link between serum and salivary cortisol concentrations was also estimated by a regression analysis. Conclusions: The reliability of both RIAs and regression found between serum and salivary cortisol concentrations permits the validation of saliva-sampling as a noninvasive technique for cortisol level assessment in horses. |
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Blackwell Publishing Ltd |
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Edition |
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| ISSN |
2042-3306 |
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Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
5428 |
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| Author |
Skandakumar, S.; Stodulski, G.; Hau, J. |
| Title |
Salivary IgA: a Possible Stress Marker In Dogs |
Type |
Abstract |
| Year |
1995 |
Publication |
Animal Welfare |
Abbreviated Journal |
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| Volume |
4 |
Issue |
4 |
Pages |
339-350 |
| Keywords |
Animal Welfare; Behaviour; Cortisol; Dog; Salivary Iga (S-Iga); Stress; Well-Being |
| Abstract |
Stress in humans has been reported to be associated with a decrease in the salivary immunoglobulin A (s-IgA) levels enabling the possible use of s-IgA to assess stress. Prolonged stress, if reliably assessed in a non-invasive manner, may be used to assess animal welfare. This study analysed groups of dogs undergoing physical and temperamental training and s-IgA levels were measured by rocket immunoelectrophoresis in prospective samples. Behavioural assessment was carried out and cortisol levels in saliva were measured by ELISA. A significant negative correlation (P < 0.007) between the logarithmic cortisol concentrations and s-IgA levels in saliva was recorded. The behavioural assessment of the dogs agreed well with the biochemical markers. It is concluded that IgA levels in saliva may be a useful marker of dog well-being and that stress results in decreased s-IgA levels. |
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no |
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Equine Behaviour @ team @ |
Serial |
5964 |
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| Author |
Shanahan, S. |
| Title |
Trailer loading stress in horses: behavioral and physiological effects of nonaversive training (TTEAM) |
Type |
Journal Article |
| Year |
2003 |
Publication |
Journal of Applied Animal Welfare Science : JAAWS |
Abbreviated Journal |
J Appl Anim Welf Sci |
| Volume |
6 |
Issue |
4 |
Pages |
263-274 |
| Keywords |
Animals; *Conditioning, Operant; *Escape Reaction; Female; Heart Rate; Horses/*psychology; Hydrocortisone/metabolism; Male; Saliva/metabolism; Stress/metabolism/prevention & control/*veterinary; *Transportation |
| Abstract |
Resistance in the horse to trailer loading is a common source of stress and injury to horses and their handlers. The objective of this study was to determine whether nonaversive training based on the Tellington-Touch Equine Awareness Method (TTEAM; Tellington-Jones &Bruns, 1988) would decrease loading time and reduce stress during loading for horses with a history of reluctance to load. Ten horses described by their owners as “problem loaders” were subjected to pretraining and posttraining assessments of loading. Each assessment involved two 7-min loading attempts during which heart rate and saliva cortisol were measured. The training consisted of six 30-min sessions over a 2-week period during which the horse and owner participated in basic leading exercises with obstacles simulating aspects of trailering. Assessment showed heart rate and saliva cortisol increased significantly during loading as compared to baseline (p <.001 and p <.05, respectively). Reassessment after training showed a decrease in loading time (p <.02), reduced heart rate during loading (p <.002), and reduced saliva cortisol as compared to pretraining assessments. Seven “good loaders” also were subject to loading assessment for physiological comparison. Increases in heart rate during loading were significantly higher in the good loaders (p <.001). Nonaversive training simulating aspects of loading may effectively reduce loading time and stress during loading for horses with a history of resistance to trailer loading. |
| Address |
shanahandvm@yahoo.ca |
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English |
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Series Issue |
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Edition |
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| ISSN |
1088-8705 |
ISBN |
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| Notes |
PMID:14965781 |
Approved |
no |
| Call Number |
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Serial |
1903 |
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| Author |
van der Kolk, J.H.; Nachreiner, R.F.; Schott, H.C.; Refsal, K.R.; Zanella, A.J. |
| Title |
Salivary and plasma concentration of cortisol in normal horses and horses with Cushing's disease |
Type |
Journal Article |
| Year |
2001 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
33 |
Issue |
2 |
Pages |
211-213 |
| Keywords |
Adrenal Cortex Function Tests/standards/veterinary; Animals; Cushing Syndrome/diagnosis/metabolism/*veterinary; Female; Horse Diseases/blood/*diagnosis/metabolism; Horses/blood/*metabolism; Hydrocortisone/blood/*metabolism; Male; Predictive Value of Tests; Reference Values; Saliva/*metabolism |
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Department of Large Animal Medicine and Nutrition, Veterinary Faculty, Utrecht University, The Netherlands |
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English |
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Series Issue |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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Conference |
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| Notes |
PMID:11266074 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4281 |
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| Author |
Alexander, F.; Nicholson, J.D. |
| Title |
The blood and saliva clearances of phenobarbitone and pentobarbitone in the horse |
Type |
Journal Article |
| Year |
1968 |
Publication |
Biochemical pharmacology |
Abbreviated Journal |
Biochem Pharmacol |
| Volume |
17 |
Issue |
2 |
Pages |
203-210 |
| Keywords |
Animals; Female; *Horses; Injections, Intravenous; Male; Metabolic Clearance Rate; Pentobarbital/blood/*metabolism; Phenobarbital/blood/*metabolism; Protein Binding; *Saliva; Time Factors |
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English |
Summary Language |
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0006-2952 |
ISBN |
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| Notes |
PMID:5647047 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
117 |
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| Author |
Dreschel, N.A.; Granger, D.A. |
| Title |
Methods of collection for salivary cortisol measurement in dogs |
Type |
Journal Article |
| Year |
2009 |
Publication |
Hormones and Behavior |
Abbreviated Journal |
Horm. Behav. |
| Volume |
55 |
Issue |
1 |
Pages |
163-168 |
| Keywords |
Dog; Canine; Salivary cortisol; Methods; Measurement; Stress |
| Abstract |
Salivary cortisol has been increasingly used as a measure of stress response in studies of welfare, reaction to stress and human–animal interactions in dogs and other species. While it can be a very useful measure, there are a number of saliva collection issues made evident through studies in the human and animal fields which have not been investigated in the canine species. Collection materials and the volume of saliva that is collected; the use of salivary stimulants; and the effect of food contamination can all dramatically impact cortisol measurement, leading to spurious results. In order to further examine the limitations of the collection method and the effects of collection material and salivary stimulant on salivary cortisol levels, a series of clinical, in vitro and in vivo studies were performed. It was found that there is a large amount of inter- and intra-individual variation in salivary cortisol measurement. Beef flavoring of collection materials leads to unpredictable variability in salivary cortisol concentration. Using salivary stimulants such as citric acid also has the potential to affect cortisol concentration measurement in saliva. Hydrocellulose appears to be a useful collection material for salivary cortisol determination. Recommendations for collection materials and use of salivary stimulants are presented. |
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| ISSN |
0018-506x |
ISBN |
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Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
5560 |
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| Author |
Vetvik, H.; Grewal, H.M.S.; Haugen, I.L.; Åhrén, C.; Haneberg, B. |
| Title |
Mucosal antibodies can be measured in air-dried samples of saliva and feces |
Type |
Journal Article |
| Year |
1998 |
Publication |
Journal of Immunological Methods |
Abbreviated Journal |
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| Volume |
215 |
Issue |
1–2 |
Pages |
163-172 |
| Keywords |
Saliva; Feces; IgA; IgG; Air-drying |
| Abstract |
IgA antibodies reflecting airways or intestinal mucosal immune responses can be found in saliva and feces, respectively, and IgG antibodies reflecting serum antibodies can be found in saliva. In this study, antibodies were detected in samples of saliva and feces which had been air-dried at room temperature (+20°C) or +37°C, and stored at these temperatures for up to 6 months. In saliva the antibody levels increased, while the antibodies in feces decreased upon storage. The individual IgA antibody concentrations which were adjusted by using the ratios of specific IgA/total IgA were relatively stable in both saliva and feces, and correlated with corresponding antibody levels in samples which had been stored at -20°C. The results indicate that air-dried saliva and feces can be used for semiquantitative measurements of mucosal antibodies, even after prolonged storage at high temperatures and lack of refrigeration. |
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Edition |
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0022-1759 |
ISBN |
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no |
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Equine Behaviour @ team @ |
Serial |
5996 |
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