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Author (up) Casella, S.; Fazio, F.; Giannetto, C.; Giudice, E.; Piccione, G.
Title Influence of transportation on serum concentrations of acute phase proteins in horse Type Journal Article
Year 2012 Publication Research in Veterinary Science Abbreviated Journal
Volume 93 Issue 2 Pages 914-917
Keywords Acute phase proteins; Horse; Transportation stress; Welfare; White Blood Cells
Abstract The modifications of Haptoglobin (Hp), Serum Amyloid A (SAA), Fibrinogen (Fbg) and White Blood Cells (WBCs) were evaluated in 15 Saddle Italian horses. Ten horses were transported covering a distance of about 320 km within 4 h with an average speed of 80 km/h (experimental group) and five horses were not subject to transportation (control group). Blood was collected via jugular venipuncture before the transportation (T0), immediately after the transportation (T1), 12 (T12), 24 (T24) and 48 (T48) hours after the transportation in experimental group and at the same time point in control group. For each parameter statistical analysis of different groups and sampling time was performed using a two-way analysis of covariance, with the data before the transportation (T0) as the covariate, by the GLM procedure of SAS. For all parameters the interaction (Group × Time) was tested and it was resulted no significant. The application of statistical analysis showed significant differences between the control group and horses subjected to transportation (P < 0.01), and the influence of sampling time (P < 0.05) on Hp, SAA and WBCs. These modifications appeared to be innovative showing that equine Hp, generally considered as moderate acute phase protein, increases more rapidly than the SAA after transportation-induced stress.
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ISSN 0034-5288 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5843
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Author (up) Cassiat, G.; Pourcelot, P.; Tavernier, L.; Geiger, D.; Denoix, J.M.; Degueurce, D.
Title Influence of individual competition level on back kinematics of horses jumping a vertical fence Type Journal Article
Year 2004 Publication Equine Veterinary Journal Abbreviated Journal Equine Vet J
Volume 36 Issue 8 Pages 748-753
Keywords Animals; Back/*physiology; Biomechanics; Female; Forelimb/*physiology; Gait/*physiology; Hindlimb/*physiology; Horses/*physiology; Image Processing, Computer-Assisted; Imaging, Three-Dimensional/veterinary; Locomotion/physiology; Male; Video Recording
Abstract REASONS FOR PERFORMING STUDY: The costs and investments required for the purchase and training of showjumpers justify the need to find selection means for jumping horses. Use of objective kinematic criteria correlated to jumping ability could be helpful for this assessment. OBJECTIVES: To compare back kinematics between 2 groups of horses of different competition levels (Group 1, competing at high level; Group 2 competing at low level) while free jumping over a 1 m vertical fence. METHODS: Three-dimensional recordings were performed using 2 panning cameras. Kinematic parameters of the withers and tuber sacrale (vertical displacement, vertical and horizontal velocities), backline inclination and flexion-extension motion of the 3 main dorsal segments (thoracic, thoracolumbar and lumbosacral) were analysed. RESULTS: Group 2 horses had a lower displacement of their withers and tuber sacrale from the end of the last approach stride until the first departure stride (P<0.05). As a result, they increased the flexion of their thoracolumbar and lumbosacral junctions during the hindlimb swing phase before take-off (P<0.05). However, withers and tuber sacrale velocities were slightly modified. Group 1 horses pitched their backline less forward during the forelimb stance phase before take-off and straightened it more after landing (P<0.05), probably indicating a more efficient strutting action of their forelimbs. CONCLUSIONS AND POTENTIAL RELEVANCE: Because significant differences in back motion were found between good and poor jumpers when jumping a 1 m high fence, criteria based on certain back kinematics can be developed that may help in the selection of talented showjumpers.
Address UMR INRA-ENVA de Biomecanique et Pathologie Locomotrice du Cheval, Ecole Nationale Veterinaire d'Alfort, Avenue du General de Gaulle, 94704 Maisons Alfort, France
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Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0425-1644 ISBN Medium
Area Expedition Conference
Notes PMID:15656509 Approved no
Call Number Equine Behaviour @ team @ Serial 4042
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Author (up) Cayado, P.; Munoz-Escassi, B.; Dominguez, C.; Manley, W.; Olabarri, B.; Sanchez de la Muela, M.; Castejon, F.; Maranon, G.; Vara, E.
Title Hormone response to training and competition in athletic horses Type Journal Article
Year 2006 Publication Equine Veterinary Journal. Supplement Abbreviated Journal Equine Vet J Suppl
Volume Issue 36 Pages 274-278
Keywords Adrenocorticotropic Hormone/*blood; Animals; Case-Control Studies; *Horses/blood/physiology/psychology; Hydrocortisone/*blood; Physical Conditioning, Animal/*physiology; Sports; *Stress, Psychological
Abstract REASONS FOR PERFORMING STUDY: It is recognised that the amount of psychological stress that an animal encounters determines the degree of response of the hypothalamic-pituitary-adrenal (HPA) axis. In human athletes, the added emotive stress of competition is an important element in the adrenal response. The aim of this study was to examine the effect of show-jumping as well as dressage on stress levels by comparing horses' stress response at a horse show compared to their familiar home. METHODS: Fifty-one horses involved in competition were used. EDTA blood samples were collected before exercise, upon arrived to the schooling area (control), and k over a jump or dressage course. After sampling, plasma was separated and stored at -80 degrees C until determinations of cortisol and ACTH were performed. Fourteen healthy horses not involved in competition were used as control group. RESULTS: Competition induced a significant increase in cortisol and ACTH responses in both, jumping and dressage horses and this effect was more apparent in dressage horses. When horses were most experienced, cortisol and ACTH responses were much lower. CONCLUSION: This study shows that competition elicits a classic physiological stress response in horses and that different training programmes induce different responses. It suggests that horses involved in competition can provide a good model to study the exercise-induced stress response.
Address Hospital SL., Madrid, Spain
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Notes PMID:17402431 Approved no
Call Number Equine Behaviour @ team @ Serial 3705
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Author (up) Cerasoli, F.; Podaliri Vulpiani, M.; Saluti, G.; Conte, A.; Ricci, M.; Savini, G.; D'Alterio, N.
Title Assessment of Welfare in Groups of Horses with Different Management, Environments and Activities by Measuring Cortisol in Horsehair, Using Liquid Chromatography Coupled to Hybrid Orbitrap High-Resolution Mass Spectrometry Type
Year 2022 Publication Animals Abbreviated Journal Animals
Volume 12 Issue 14 Pages
Keywords cortisol; animal welfare; horse; Lc-Hrms/Ms
Abstract Horses have always been animals used for companionship, work, transportation, and performance purposes over the history of humanity; there are different ways of managing horses, but studies on how horse welfare is influenced by different activities and managements are scanty. Understanding how the management, the environment, and the different uses of horses can affect the level of stress and well-being is important not only for people associated with horses. Three groups of horses with different management, environments, and activities were selected: (1) stabled horses ridden frequently, (2) horses that perform public order service under the Italian state police, and (3) free-ranging horses. Cortisol analysis was carried out on horsehair samples using liquid chromatography coupled to hybrid orbitrap high-resolution mass spectrometry (LC-HRMS/MS), a laboratory technique used for the first time to quantify horsehair cortisol. The selection of horses to be included in the three groups was carried out by including only subjects with positive welfare assessment in accordance with the horse welfare assessment protocol (AWIN). These analyses demonstrated that the cortisol levels detected in the horsehair of free-ranging animals were significantly higher compared to those detected in stabled and working horses. These results may have been a consequence of complex environmental, managerial, and behavioral factors, which should be worth further investigation
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Series Editor Series Title Abbreviated Series Title Animals
Series Volume 12 Series Issue 14 Edition
ISSN 2076-2615 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 6674
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Author (up) Chaya, L.; Cowan, E.; McGuire, B.
Title A note on the relationship between time spent in turnout and behaviour during turnout in horses (Equus caballus) Type Journal Article
Year 2006 Publication Applied Animal Behaviour Science Abbreviated Journal Appl. Anim. Behav. Sci.
Volume 98 Issue 1-2 Pages 155-160
Keywords Horse; Behaviour; Turnout; Welfare
Abstract We examined if time spent in turnout influenced behaviour during turnout for horses maintained in stalls and given either 2 h/week (n = 7) or 12 h/week (n = 7) of turnout. Horses turned out for 2 h/week were more likely than those turned out for 12 h/week to trot, canter, and buck. Frequency of trotting and cantering was also higher and frequency of grazing lower in horses turned out for 2 h/week. These results have welfare implications and support previous studies showing that horses react to confinement with increased activity when not confined.
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ISSN 0168-1591 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 4815
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Author (up) Chiba, K.; Ikai, A.; Kawamura-Konishi, Y.; Kihara, H.
Title Kinetic study on myoglobin refolding monitored by five optical probe stopped-flow methods Type Journal Article
Year 1994 Publication Proteins Abbreviated Journal Proteins
Volume 19 Issue 2 Pages 110-119
Keywords Animals; Chromatography, Gel; Circular Dichroism; Horses; Kinetics; Metmyoglobin/analogs & derivatives/chemistry; Myoglobin/*chemistry; *Protein Folding; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Urea
Abstract The refolding kinetics of horse cyanometmyoglobin induced by concentration jump of urea was investigated by five optical probe stopped-flow methods: absorption at 422 nm, tryptophyl fluorescence at around 340 nm, circular dichroism (CD) at 222 nm, CD at 260 nm, and CD at 422 nm. In the refolding process, we detected three phases with rate constants of > 1 x 10(2) s-1, (4.5-9.3) s-1, and (2-5) x 10(-3) s-1. In the fastest phase, a substantial amount of secondary structure (approximately 40%) is formed within the dead time of the CD stopped-flow apparatus (10.7 ms). The kinetic intermediate populated in the fastest phase is shown to capture a hemindicyanide, suggesting that a “heme pocket precursor” recognized by hemindicyanide must be constructed within the dead time. In the middle phase, most of secondary and tertiary structures, especially around the captured hemindicyanide, have been constructed. In the slowest phase, we detected a minor structural rearrangement accompanying the ligand-exchange reaction in the fifth coordination of ferric iron. We present a possible model for the refolding process of myoglobin in the presence of the heme group.
Address Laboratory of Biodynamics, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Kanagawa, Japan
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ISSN 0887-3585 ISBN Medium
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Notes PMID:8090705 Approved no
Call Number Equine Behaviour @ team @ Serial 3799
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Author (up) Chilton, N.B.
Title The use of nuclear ribosomal DNA markers for the identification of bursate nematodes (order Strongylida) and for the diagnosis of infections Type Journal Article
Year 2004 Publication Animal Health Research Reviews / Conference of Research Workers in Animal Diseases Abbreviated Journal Anim Health Res Rev
Volume 5 Issue 2 Pages 173-187
Keywords Animals; Birds; Cats; DNA Primers; DNA, Helminth/*analysis; DNA, Ribosomal/*analysis; Dogs; Horses; Molecular Diagnostic Techniques/veterinary; Ruminants; Strongylida/*genetics; Strongylida Infections/diagnosis/*veterinary
Abstract Many bursate nematodes are of major importance to animal health. Animals are often parasitized by multiple species that differ in their prevalence, relative abundance and/or pathogenicity. Implementation of effective management strategies for these parasites requires reliable methods for their detection in hosts, identification to the species level and measurement of intensity of infection. One major problem is the difficulty of accurately identifying and distinguishing many species of bursate nematode because of the remarkable morphological similarity of their eggs and larvae. The inability to identify, with confidence, individual nematodes (irrespective of their life-cycle stage) to the species level by morphological methods has often led to a search for species-specific genetic markers. Studies over the past 15 years have shown that sequences of the internal transcribed spacers of ribosomal DNA provide useful genetic markers, providing the basis for the development of PCR-based diagnostic tools. Such molecular methods represent powerful tools for studying the systematics, epidemiology and ecology of bursate nematodes and, importantly, for the specific diagnosis of infections in animals and humans, thus contributing to improved control and prevention strategies for these parasites.
Address Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada. neil.chilton@usask.ca
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ISSN 1466-2523 ISBN Medium
Area Expedition Conference
Notes PMID:15984323 Approved no
Call Number Equine Behaviour @ team @ Serial 2628
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Author (up) Chmel, L.; Hasilikova, A.; Hrasko, J.; Vlacilikova, A.
Title The influence of some ecological factors on keratinophilic fungi in the soil Type Journal Article
Year 1972 Publication Sabouraudia Abbreviated Journal Sabouraudia
Volume 10 Issue 1 Pages 26-34
Keywords Animals; Arthrodermataceae/growth & development/isolation & purification/metabolism; Carbohydrates; Czechoslovakia; Ecology; Fungi/growth & development/*isolation & purification/metabolism; Hair; Horses; Humic Substances; Humidity; Keratins/metabolism; Microsporum/isolation & purification; Mitosporic Fungi/isolation & purification; Phosphates; Seasons; Soil; *Soil Microbiology; Species Specificity; Temperature; Trichophyton/isolation & purification
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ISSN 0036-2174 ISBN Medium
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Notes PMID:5063162 Approved no
Call Number Equine Behaviour @ team @ Serial 2719
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Author (up) Cho, K.C.; Chan, K.K.
Title Kinetics of cold-induced denaturation of metmyoglobin Type Journal Article
Year 1984 Publication Biochimica et Biophysica Acta (BBA) – Protein Structure and Molecular Enzymology Abbreviated Journal
Volume 786 Issue 1-2 Pages 103-108
Keywords Metmyoglobin denaturation; Temperature jump; Denaturation kinetics; Conformational transformation; (Horse heart)
Abstract Using a slow temperature-jump spectrophotometer, we have studied the kinetics of cold-induced denaturation of metmyoglobin between 0[degree sign]C and 20[degree sign]C at acidic pH. The time-scale of the transition is slow and is of the order of minutes. The results are consistent with the transition's involving a total of three states, native (N), transient intermediate (I) and denatured (D), which are converted from one to the other in that order.
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Notes Approved no
Call Number refbase @ user @ Serial 3978
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Author (up) Christensen, H.A.; Herrer, A.
Title Attractiveness of sentinel animals to vectors of leishmaniasis in Panama Type Journal Article
Year 1973 Publication The American Journal of Tropical Medicine and Hygiene Abbreviated Journal Am J Trop Med Hyg
Volume 22 Issue 5 Pages 578-584
Keywords Animals; Cricetinae; Dogs; Ecology; Horses; *Insect Vectors; Leishmania/isolation & purification; Leishmaniasis, Mucocutaneous/*etiology; Male; Panama; *Phlebotomus; Rats
Abstract
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Language English Summary Language Original Title
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ISSN 0002-9637 ISBN Medium
Area Expedition Conference
Notes PMID:4729738 Approved no
Call Number Equine Behaviour @ team @ Serial 2712
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