| Records |
| Author |
Traversa, D.; Giangaspero, A.; Galli, P.; Paoletti, B.; Otranto, D.; Gasser, R.B. |
| Title |
Specific identification of Habronema microstoma and Habronema muscae (Spirurida, Habronematidae) by PCR using markers in ribosomal DNA |
Type |
Journal Article |
| Year |
2004 |
Publication |
Molecular and Cellular Probes |
Abbreviated Journal |
Mol Cell Probes |
| Volume |
18 |
Issue |
4 |
Pages |
215-221 |
| Keywords |
Animals; Base Sequence; DNA, Ribosomal/blood/*genetics; Feces/parasitology; Genetic Markers; Horses/*parasitology; Molecular Sequence Data; Muscidae/*genetics; Polymerase Chain Reaction; Spirurida Infections/genetics; Spiruroidea/*genetics; Stomach/*parasitology |
| Abstract |
Gastric or cutaneous habronemosis caused by Habronema microstoma Creplin, 1849 and Habronema muscae Carter, 1865 is a parasitic disease of equids transmitted by muscid flies. There is a paucity of information on the epidemiology of this disease, which is mainly due to limitations with diagnosis in the live animal and with the identification of the parasites in the intermediate hosts. To overcome such limitations, a molecular approach, based on the use of genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal DNA, was established for the two species of Habronema. Characterisation of the ITS-2 revealed sequence lengths and G+C contents of 296 bp and 29.5% for H. microstoma, and of 334 bp and 35.9% for H. muscae, respectively. Exploiting the sequence difference (approximately 40%) between the two species of nematode, primers were designed and tested by the polymerase chain reaction (PCR) for their specificity using a panel of control DNA samples from common equid endoparasites, and from host tissues, faeces or muscid flies. Effective amplification from each of the two species of Habronema was achieved from as little as 10 pg of genomic DNA. Hence, this molecular approach allows the specific identification and differentiation of the DNA from H. microstoma and H. muscae, and could thus provide a molecular tool for the specific detection of Habronema DNA (irrespective of developmental stage) from faeces, skin and muscid fly samples. The establishment of this tool has important implications for the specific diagnosis of clinical cases of gastric and cutaneous habronemosis in equids, and for studying the ecology and epidemiology of the two species of Habronema. |
| Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy |
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Place of Publication |
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| Language |
English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0890-8508 |
ISBN |
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Expedition |
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Conference |
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| Notes |
PMID:15271381 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2634 |
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| Author |
Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B. |
| Title |
Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces |
Type |
Journal Article |
| Year |
2004 |
Publication |
Parasitology |
Abbreviated Journal |
Parasitology |
| Volume |
129 |
Issue |
Pt 6 |
Pages |
733-739 |
| Keywords |
Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics |
| Abstract |
Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae. |
| Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it |
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Place of Publication |
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| Language |
English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
0031-1820 |
ISBN |
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Medium |
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| Area |
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Expedition |
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Conference |
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| Notes |
PMID:15648696 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2631 |
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| Author |
Turner, J.W.J.; Liu, I.K.; Kirkpatrick, J.F. |
| Title |
Remotely delivered immunocontraception in free-roaming feral burros (Equus asinus) |
Type |
Journal Article |
| Year |
1996 |
Publication |
Journal of reproduction and fertility |
Abbreviated Journal |
J Reprod Fertil |
| Volume |
107 |
Issue |
1 |
Pages |
31-35 |
| Keywords |
Animals; *Animals, Wild; Contraception, Immunologic/methods/*veterinary; *Equidae; Feces/chemistry; Female; Pregnancy; Pregnancy Tests; Swine; Zona Pellucida/immunology |
| Abstract |
Regulation of local overpopulations of free-roaming feral equids is in demand worldwide for ecological balance and habitat preservation. Contraceptive vaccines have proven effective in feral horses, which breed seasonally, but no data are available for equids such as the burro, which is reproductively active all year round. In the present study, 27 individually identified female feral burros (Equus asinus) roaming free in Virgin Islands National Park (St John, US Virgin Islands; Lesser Antilles) were remotely treated with pig zonae pellucidae (PZP) vaccine. Between January and May, 16 burros were darted with a 1 ml emulsion of PZP plus Freund's adjuvant. Ten to twelve months later each treated burro was given a single booster injection of PZP plus adjuvant to maintain contraception through a second year. Eleven adult untreated jennies served as controls. Beginning one year after initial vaccination, these burros were monitored for pregnancy and foal production. Collection of data to determine treatment effect was not begun until 12 months after initial treatment to ensure that pregnancies existing before vaccination were not included. Pregnancy was assessed using previously validated methods for steroid metabolite measurement in fresh faecal samples. None of the PZP-treated burros produced foals between 0 and 12 months after the last inoculation. One PZP-treated burro tested positive for pregnancy at 10 months after the final inoculation. During this same period, six of 11 untreated burros tested pregnancy-positive, and four were observed with foals. There was no difference in pregnancy rates among treated, control and randomly sampled jennies between 12 and 24 months after the last inoculation. The results demonstrate that, in free-roaming feral burros that are reproductively active all year round: (1) burros can be accessed for remotely delivered PZP vaccination; (2) PZP contraception is effective; (3) PZP contraception is reversible; and (4) pregnancy can be reliably detected by faecal steroid analysis. |
| Address |
Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo 43699, USA |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0022-4251 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:8699431 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
144 |
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| Author |
Vetvik, H.; Grewal, H.M.S.; Haugen, I.L.; Åhrén, C.; Haneberg, B. |
| Title |
Mucosal antibodies can be measured in air-dried samples of saliva and feces |
Type |
Journal Article |
| Year |
1998 |
Publication |
Journal of Immunological Methods |
Abbreviated Journal |
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| Volume |
215 |
Issue |
1–2 |
Pages |
163-172 |
| Keywords |
Saliva; Feces; IgA; IgG; Air-drying |
| Abstract |
IgA antibodies reflecting airways or intestinal mucosal immune responses can be found in saliva and feces, respectively, and IgG antibodies reflecting serum antibodies can be found in saliva. In this study, antibodies were detected in samples of saliva and feces which had been air-dried at room temperature (+20°C) or +37°C, and stored at these temperatures for up to 6 months. In saliva the antibody levels increased, while the antibodies in feces decreased upon storage. The individual IgA antibody concentrations which were adjusted by using the ratios of specific IgA/total IgA were relatively stable in both saliva and feces, and correlated with corresponding antibody levels in samples which had been stored at -20°C. The results indicate that air-dried saliva and feces can be used for semiquantitative measurements of mucosal antibodies, even after prolonged storage at high temperatures and lack of refrigeration. |
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Abbreviated Series Title |
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Edition |
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| ISSN |
0022-1759 |
ISBN |
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Medium |
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Conference |
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| Notes |
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Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
5996 |
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| Author |
Wilhelm, W.E.; Anderson, J.H. |
| Title |
Vahlkampfia lobospinosa (Craig. 1912) Craig. 1913: rediscovery of a coprozoic ameba |
Type |
Journal Article |
| Year |
1971 |
Publication |
The Journal of Parasitology |
Abbreviated Journal |
J Parasitol |
| Volume |
57 |
Issue |
6 |
Pages |
1378-1379 |
| Keywords |
Animals; Cattle; Ecology; Feces/microbiology; Horse Diseases/epidemiology; Horses; Protozoan Infections/epidemiology; *Protozoan Infections, Animal; Sarcodina/*classification/growth & development; Swine; Swine Diseases/epidemiology; Tennessee |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0022-3395 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:5157177 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2724 |
| Permanent link to this record |
