| Records |
| Author |
Polley, L. |
| Title |
Strongylid parasites of horses: experimental ecology of the free-living stages on the Canadian prairie |
Type |
Journal Article |
| Year |
1986 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
| Volume |
47 |
Issue |
8 |
Pages |
1686-1693 |
| Keywords |
Animals; Canada; Ecology; Feces; Female; Horse Diseases/*epidemiology/parasitology; Horses; Larva; Ovum/cytology; Seasons; Strongyloides/isolation & purification; Strongyloidiasis/epidemiology/*veterinary |
| Abstract |
Each month for a 1-year period (October through September), equine fecal masses containing eggs of strongylid nematodes were placed outdoors on small grass plots in Saskatchewan, Canada. Thereafter, feces and grass from the plots were sampled after intervals of 1 week or longer, and the strongylid eggs and larvae recovered were counted. These observations were made over a 2-year period. Development of eggs to infective larvae occurred in all experiments, except those established in October, December, and January. Infective larvae from experiments set up in April through September survived that winter. During the summer, there was a gradual build up of infective larvae in the fecal masses, which reached a peak in August and September and then decreased into the winter. These results are discussed in the context of the control of strongylid parasites of horses on the Canadian prairie and in other areas of the world with a similar climate and similar horse management practices. |
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Place of Publication |
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Editor |
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| Language |
English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0002-9645 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:3752676 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2682 |
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| Author |
Schwarzenberger, F.; Mostl, E.; Bamberg, E.; Pammer, J.; Schmehlik, O. |
| Title |
Concentrations of progestagens and oestrogens in the faeces of pregnant Lipizzan, trotter and thoroughbred mares |
Type |
Journal Article |
| Year |
1991 |
Publication |
Journal of reproduction and fertility. Supplement |
Abbreviated Journal |
J Reprod Fertil Suppl |
| Volume |
44 |
Issue |
|
Pages |
489-499 |
| Keywords |
Animals; Estrogens/*analysis; Feces/*chemistry; Female; Gestational Age; Horses/*metabolism; Immunoenzyme Techniques; Labor, Obstetric; Pregnancy; Pregnancy, Animal/*metabolism; Pregnenes/analysis; Progestins/*analysis |
| Abstract |
Faecal samples were collected at weekly intervals from pregnant Lipizzan mares during Weeks 7-16 following mating and from Lipizzan, Trotter and Thoroughbred mares during the last 3 months of gestation. After parturition, samples were taken daily from the Thoroughbred mares for another 6 days. Non-pregnant mares served as controls. The concentrations of unconjugated oestrogens (Eg), 20 alpha-OH-progestagens (20 alpha-G) and 20 beta-OH-progestagens (20 beta-G) were measured by enzyme immunoassay. In the faeces of Lipizzan mares, immunoreactive progestagens were significantly (P less than 0.01) elevated above the levels in non-pregnant mares by Week 11, and Eg by Week 13 of pregnancy onwards. During the last 3 months of gestation, concentrations of Eg were significantly higher in Trotter mares than in Lipizzan and Thoroughbred mares. Concentrations of 20 alpha-G and 20 beta-G increased to maximal values in the last month of gestation. There was no significant difference among the 3 breeds with respect to 20 alpha-G but, during the 10 weeks before parturition, concentrations of 20 beta-G in the Lipizzan mares were significantly lower (P less than 0.05) than those in the Thoroughbred mares. They were also significantly lower than those of the Trotter mares during the last 4 weeks of gestation. After parturition, the concentrations of Eg and progestagens had declined to baseline values by Days 3 and 4 respectively. From these results we conclude that high concentrations of progestagens with 20 alpha- and 20 beta-hydroxyl groups are present in the faeces of pregnant mares, especially during the last month of gestation. |
| Address |
Institut fur Biochemie, Veterinary Medical University, Vienna, Austria |
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Thesis |
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Place of Publication |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0449-3087 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:1795293 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
322 |
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| Author |
Stahlbaum, C.C.; Houpt, K.A. |
| Title |
The role of the Flehmen response in the behavioral repertoire of the stallion |
Type |
Journal Article |
| Year |
1989 |
Publication |
Physiology & behavior |
Abbreviated Journal |
Physiol. Behav. |
| Volume |
45 |
Issue |
6 |
Pages |
1207-1214 |
| Keywords |
Animals; Behavior, Animal/*physiology; Circadian Rhythm; Discrimination (Psychology)/physiology; Estrus; Feces; Female; Horses/*physiology; Male; Sexual Behavior, Animal/*physiology; Smell/*physiology; Taste/physiology; Urine |
| Abstract |
The role of the Flehmen response in equine behavior was investigated under field and laboratory conditions. In Experiment 1, a field study made of five stallions on pasture with between three and eighteen mares each during the season indicated the following: 1) The Flehmen response was most frequently preceded by nasal, rather than oral, investigation of substances; 2) The stallions' rate of Flehmen varied with the estrous cycles of the mares; 3) The rate of Flehmen response did not show a variation with time of day; and 4) The Flehmen response was most frequently followed by marking behaviors rather than courtship behaviors. The results suggest that the Flehmen response is not an immediate component of sexual behavior, e.g., courtship of the stallion but may be involved in the overall monitoring of the mare's estrous cycle. Therefore the Flehmen response may contribute to the chemosensory priming of the stallion for reproduction. In Experiment 2 stallions were presented with urine or feces of mares in various stages of the reproductive cycle as well as with their own or other males' urine or feces. The occurrence of sniffing and Flehmen was used to determine the discriminatory ability of the stallions. Stallions can differentiate the sex of a horse on the basis of its feces alone, but cannot differentiate on the basis of urine. This ability may explain the function of fecal marking behavior of stallions. |
| Address |
New York State College of Veterinary Medicine, Cornell University, Ithaca 14853 |
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Place of Publication |
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Editor |
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| Language |
English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0031-9384 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:2813545 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
44 |
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| Author |
Taberlet, P.; Waits, L.P.; Luikart, G. |
| Title |
Noninvasive genetic sampling: look before you leap |
Type |
Journal Article |
| Year |
1999 |
Publication |
Trends in Ecology & Evolution |
Abbreviated Journal |
Trends Ecol. Evol |
| Volume |
14 |
Issue |
8 |
Pages |
323-327 |
| Keywords |
Hairs; Feces; Feathers; Allelic dropout; Individual identification; Conservation genetics; Behavioural ecology; Pilot study; Microsatellites; Probability of identity |
| Abstract |
Noninvasive sampling allows genetic studies of free-ranging animals without the need to capture or even observe them, and thus allows questions to be addressed that cannot be answered using conventional methods. Initially, this sampling strategy promised to exploit fully the existing DNA-based technology for studies in ethology, conservation biology and population genetics. However, recent work now indicates the need for a more cautious approach, which includes quantifying the genotyping error rate. Despite this, many of the difficulties of noninvasive sampling will probably be overcome with improved methodology. |
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Thesis |
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Place of Publication |
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Editor |
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Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0169-5347 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
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Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
6573 |
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| Author |
Takai, S.; Fujimori, T.; Katsuzaki, K.; Tsubaki, S. |
| Title |
Ecology of Rhodococcus equi in horses and their environment on horse-breeding farms |
Type |
Journal Article |
| Year |
1987 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
| Volume |
14 |
Issue |
3 |
Pages |
233-239 |
| Keywords |
Actinomycetales Infections/*veterinary; Animals; Animals, Newborn/*microbiology; *Environmental Microbiology; Feces/microbiology; Female; Horse Diseases/*microbiology; Horses/*microbiology; Rhodococcus/*isolation & purification |
| Abstract |
Quantitative culture of R. equi in the feces of dams and foals, in the air of the stalls and in the soil of the paddocks was carried out on three horse-breeding farms during the foaling season. The isolation rates of R. equi from the feces of dams from the 3 farms suddenly increased to approximately 80% at the end of March, when the snow in the paddocks finished melting, and remained at that level during April and May. The mean number of R. equi and the isolation rate of R. equi from the feces of dams on the farms were investigated for 5 weeks before and 5 weeks after delivery. During the 10 weeks, there were no differences in the isolation rate or in the mean number of R. equi from the feces of dams. R. equi was first isolated from the feces of the foals born in February and the middle of March at 3-4 weeks of age, on the other hand, it was first isolated from the feces of foals born in the end of March and April at 1-2 weeks of age. The number of R. equi in the soil collected from the paddocks used by dams during the winter was approximately 10(2)-10(4) g-1 of soil during the experiment. R. equi was isolated from the air in the stalls at the end of March and the number of R. equi in the air increased particularly on dry and windy days.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Address |
Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Aomori, Japan |
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Place of Publication |
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Editor |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0378-1135 |
ISBN |
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Medium |
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| Area |
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Expedition |
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Conference |
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| Notes |
PMID:3672865 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2679 |
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| Author |
Takai, S.; Narita, K.; Ando, K.; Tsubaki, S. |
| Title |
Ecology of Rhodococcus (Corynebacterium) equi in soil on a horse-breeding farm |
Type |
Journal Article |
| Year |
1986 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
| Volume |
12 |
Issue |
2 |
Pages |
169-177 |
| Keywords |
Actinomycetales/classification/*growth & development; Animals; Corynebacterium/classification/*growth & development; Feces/microbiology; Female; Horses; Serotyping; *Soil Microbiology |
| Abstract |
The ecology of Rhodococcus (Corynebacterium) equi in soil was studied on a horse-breeding farm. R. equi was cultured from soil at a depth of 0, 10, and 20 cm on the six sites of the farm at monthly intervals for 10 months from March to December of 1983. The highest numbers of R. equi were found in the surface soil. The mean number of bacteria in soil samples at every depth increased remarkably from 0 or 10(2) to 10(4) colony-forming units (CFU) g-1 of soil in the middle of April, and later decreased gradually. R. equi inoculated into six soil exudate broths prepared from surface soils at separate sites yielded suspensions with different optical densities, indicating differences in growth. The distribution of serotypes in the soil was similar to that in the horses on the farm. These findings indicated that R. equi could multiply in the soil and flourish in the cycle existing between horses and their soil environment. |
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Place of Publication |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0378-1135 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:3750818 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2683 |
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| Author |
Thiel, D.; Jenni-Eiermann, S.; Palme, R. |
| Title |
Measuring corticosterone metabolites in droppings of capercaillies (Tetrao urogallus) |
Type |
Journal Article |
| Year |
2005 |
Publication |
Annals of the New York Academy of Sciences |
Abbreviated Journal |
Ann N Y Acad Sci |
| Volume |
1046 |
Issue |
|
Pages |
96-108 |
| Keywords |
Adrenocorticotropic Hormone/administration & dosage/analysis/metabolism; Animals; Circadian Rhythm; Corticosterone/administration & dosage/*analysis/*metabolism; Feces/*chemistry; Female; Freezing; Galliformes/*metabolism; Male; Reproducibility of Results; Sex Factors; Temperature; Time Factors; Tritium/diagnostic use |
| Abstract |
The capercaillie (Tetrao urogallus), the largest grouse species in the world, is decreasing in numbers in major parts of its distribution range. Disturbances by human outdoor activities are discussed as a possible reason for this population decline. An indicator for disturbances is the increase of the glucocorticoid corticosterone, a stress hormone, which helps to cope with life-threatening situations. However, repeated disturbances might result in a long-term increase of the basal corticosterone concentration, which can result in detrimental effects like reduced fitness and survival of an animal. To measure corticosterone metabolites (CMs) noninvasively in the droppings of free-living capercaillies, first an enzyme immunoassay (EIA) in captive birds had to be selected and validated. Therefore, the excretion pattern of intravenously injected radiolabeled corticosterone was determined and 3H metabolites were characterized. High-performance liquid chromatography (HPLC) separations of the samples containing peak concentrations revealed that corticosterone was extensively metabolized. The HPLC fractions were tested in several EIAs for glucocorticoid metabolites. The physiological relevance of this method was proved after pharmacological stimulation of the adrenocortical activity. Only the recently established cortisone assay, measuring CMs with a 3,11-dione structure, detected an expressed increase of concentrations following ACTH stimulation. To set up a sampling protocol suited for the field, we examined the influence of various storage conditions and time of day on concentrations of CMs. |
| Address |
Swiss Ornithological Institute, 6204 Sempach, Switzerland. dominik.thiel@vogelwarte.ch |
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Place of Publication |
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Editor |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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| Series Volume |
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Series Issue |
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Edition |
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| ISSN |
0077-8923 |
ISBN |
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Medium |
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| Area |
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Expedition |
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Conference |
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| Notes |
PMID:16055846 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4079 |
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| Author |
Touma, C.; Palme, R. |
| Title |
Measuring fecal glucocorticoid metabolites in mammals and birds: the importance of validation |
Type |
Journal Article |
| Year |
2005 |
Publication |
Annals of the New York Academy of Sciences |
Abbreviated Journal |
Ann N Y Acad Sci |
| Volume |
1046 |
Issue |
|
Pages |
54-74 |
| Keywords |
Animals; Birds/*metabolism; Circadian Rhythm; Feces/*chemistry; Glucocorticoids/*analysis; Mammals/*metabolism; Reproducibility of Results; Seasons; Sex Factors |
| Abstract |
In recent years, the noninvasive monitoring of steroid hormone metabolites in feces of mammals and droppings of birds has become an increasingly popular technique. It offers several advantages and has been applied to a variety of species under various settings. However, using this technique to reliably assess an animal's adrenocortical activity is not that simple and straightforward to apply. Because clear differences regarding the metabolism and excretion of glucocorticoid metabolites (GCMs) exist, a careful validation for each species and sex investigated is obligatory. In this review, general analytical issues regarding sample storage, extraction procedures, and immunoassays are briefly discussed, but the main focus lies on experiments and recommendations addressing the validation of fecal GCM measurements in mammals and birds. The crucial importance of scrutinizing the physiological and biological validity of fecal GCM analyses in a given species is stressed. In particular, the relevance of the technique to detect biologically meaningful alterations in adrenocortical activity must be shown. Furthermore, significant effects of the animals' sex, the time of day, season, and different life history stages are discussed, bringing about the necessity to seriously consider possible sex differences as well as diurnal and seasonal variations. Thus, comprehensive information on the animals' biology and stress physiology should be carefully taken into account. Together with an extensive physiological and biological validation, this will ensure that the measurement of fecal GCMs can be used as a powerful tool to assess adrenocortical activity in diverse investigations on laboratory, companion, farm, zoo, and wild animals. |
| Address |
Max Planck Institute of Psychiatry, Department of Behavioral Neuroendocrinology, Kraepelinstrasse 2-10, D-80804 Munich, Germany. touma@mpipsykl.mpg.de |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0077-8923 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:16055843 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4073 |
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| Author |
Touma, C.; Palme, R.; Sachser, N. |
| Title |
Analyzing corticosterone metabolites in fecal samples of mice: a noninvasive technique to monitor stress hormones |
Type |
Journal Article |
| Year |
2004 |
Publication |
Hormones and Behavior |
Abbreviated Journal |
Horm Behav |
| Volume |
45 |
Issue |
1 |
Pages |
10-22 |
| Keywords |
Adrenal Cortex/drug effects; Adrenal Cortex Function Tests; Adrenocorticotropic Hormone/pharmacology; Analysis of Variance; Animals; Circadian Rhythm; Corticosterone/*analysis/metabolism; Dexamethasone/pharmacology; Feces/*chemistry; Female; Immunoenzyme Techniques/*methods; Male; Mice; Mice, Inbred C57BL; Models, Animal; Reproducibility of Results; Stress, Psychological/*metabolism |
| Abstract |
In small animals like mice, the monitoring of endocrine functions over time is constrained seriously by the adverse effects of blood sampling. Therefore, noninvasive techniques to monitor, for example, stress hormones in these animals are highly demanded in laboratory as well as in field research. The aim of our study was to evaluate the biological relevance of a recently developed technique to monitor stress hormone metabolites in fecal samples of laboratory mice. In total, six experiments were performed using six male and six female mice each. Two adrenocorticotropic hormone (ACTH) challenge tests, two dexamethasone (Dex) suppression tests and two control experiments [investigating effects of the injection procedure itself and the diurnal variation (DV) of glucocorticoids (GCs), respectively] were conducted. The experiments clearly demonstrated that pharmacological stimulation and suppression of adrenocortical activity was reflected accurately by means of corticosterone metabolite (CM) measurements in the feces of males and females. Furthermore, the technique proved sensitive enough to detect dosage-dependent effects of the ACTH/Dex treatment and facilitated to reveal profound effects of the injection procedure itself. Even the naturally occurring DV of GCs could be monitored reliably. Thus, our results confirm that measurement of fecal CM with the recently established 5alpha-pregnane-3beta,11beta,21-triol-20-one enzyme immunoassay is a very powerful tool to monitor adrenocortical activity in laboratory mice. Since mice represent the vast majority of all rodents used for research worldwide and the number of transgenic and knockout mice utilized as animal models is still increasing, this noninvasive technique can open new perspectives in biomedical and behavioral science. |
| Address |
Department of Behavioural Biology, University of Muenster, D-48149 Muenster, Germany. touma@uni-muenster.de |
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Thesis |
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Place of Publication |
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Editor |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0018-506X |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:14733887 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4084 |
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| Author |
Touma, C.; Sachser, N.; Mostl, E.; Palme, R. |
| Title |
Effects of sex and time of day on metabolism and excretion of corticosterone in urine and feces of mice |
Type |
Journal Article |
| Year |
2003 |
Publication |
General and Comparative Endocrinology |
Abbreviated Journal |
Gen Comp Endocrinol |
| Volume |
130 |
Issue |
3 |
Pages |
267-278 |
| Keywords |
Animals; Chromatography, High Pressure Liquid; Circadian Rhythm/*physiology; Corticosterone/*metabolism/urine; Feces/*chemistry; Female; Immunoenzyme Techniques; Kinetics; Male; Mice; Mice, Inbred C57BL; Reference Values; Sex Factors; Stress/metabolism; Time Factors; Tritium |
| Abstract |
Non-invasive techniques to monitor stress hormones in small animals like mice offer several advantages and are highly demanded in laboratory as well as in field research. Since knowledge about the species-specific metabolism and excretion of glucocorticoids is essential to develop such a technique, we conducted radiometabolism studies in mice (Mus musculus f. domesticus, strain C57BL/6J). Each mouse was injected intraperitoneally with 740 kBq of 3H-labelled corticosterone and all voided urine and fecal samples were collected for five days. In a first experiment 16 animals (eight of each sex) received the injection at 9 a.m., while eight mice (four of each sex) were injected at 9 p.m. in a second experiment. In both experiments radioactive metabolites were recovered predominantly in the feces, although males excreted significantly higher proportions via the feces (about 73%) than females (about 53%). Peak radioactivity in the urine was detected within about 2h after injection, while in the feces peak concentrations were observed later (depending on the time of injection: about 10h postinjection in experiment 1 and about 4h postinjection in experiment 2, thus proving an effect of the time of day). The number and relative abundance of fecal [3H]corticosterone metabolites was determined by high performance liquid chromatography (HPLC). The HPLC separations revealed that corticosterone was extensively metabolized mainly to more polar substances. Regarding the types of metabolites formed, significant differences were found between males and females, but not between the experiments. Additionally, the immunoreactivity of these metabolites was assessed by screening the HPLC fractions with four enzyme immunoassays (EIA). However, only a newly established EIA for 5alpha-pregnane-3beta,11beta,21-triol-20-one (measuring corticosterone metabolites with a 5alpha-3beta,11beta-diol structure) detected several peaks of radioactive metabolites with high intensity in both sexes, while the other EIAs showed only minor immunoreactivity. Thus, our study for the first time provides substantial information about metabolism and excretion of corticosterone in urine and feces of mice and is the first demonstrating a significant impact of the animals' sex and the time of day. Based on these data it should be possible to monitor adrenocortical activity non-invasively in this species by measuring fecal corticosterone metabolites with the newly developed EIA. Since mice are extensively used in research world-wide, this could open new perspectives in various fields from ecology to behavioral endocrinology. |
| Address |
Department of Behavioral Biology, Institute of Neuro and Behavioral Biology, University of Muenster, Badestrasse 9, D-48149 Muenster, Germany. touma@uni-muenster.de |
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Place of Publication |
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Editor |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0016-6480 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:12606269 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4086 |
| Permanent link to this record |
